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Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

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Journal of Oceanology and Limnology 2022年第3期40卷 1148-1159页

作者： yunke zhu Xinran WANG Bin SUN Xianghai TANG Yunxiang MAO Key Laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao 266003China MOE Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya 572022China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The phosphatidylinositol(PI)signaling system,a central regulator of eukaryotic metabolism,is widely found in eukaryotes for regulating a variety of cell *** of the genes in the PI signaling system were found conserved in Pyropia *** this experiment,wortmannin was used as an inhibitor to inhibit the activity of phosphatidylinositol-3 kinase(PI3K),an important regulator of the PI signaling *** wortmannin treatment,the mitotic division of *** was signifi cantly inhibited in a dose-dependent manner,and the mitotic division percentage was reduced by 68.1%and 91.9%in the 5-and 10-μmol/L groups,*** thalli were treated with wortmannin,the levels of reactive oxygen species(ROS)were signifi cantly ***,the expression level of PI3K was inhibited and the expression levels of downstream genes regulated by PI3K was signifi cantly *** the PI3K-AGC signaling pathway,the expression levels of Serine/threonine protein kinase(AGC)and cyclindependent kinases A(CDKA)were downregulated,while WEE1 kinase gene(WEE1)was *** nicotinamide adenine dinucleotide phosphate(NADPH)oxidase genes were downregulated after wortmannin *** results indicate that the PI signaling system plays an important role in the regulation of cell activity in *** was speculated that the growth and development of *** might be regulated by *** PI3K,which promoted the expression of the AGC gene and further regulates the expression of downstream WEE1 and CDKA genes to advance mitotic division,and also promoted the expression level of NADPH oxidase that regulates ROS homeostasis.

关键词： Pyropia yezoensis phosphatidylinositol signaling system reactive oxygen species mitotic division

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Comparison in structure and predicted function of epiphytic bacteria on Neopyropia yezoensis and Neopyropia katadae

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Journal of Oceanology and Limnology 2023年第6期41卷 2232-2248页

作者： Xinran WANG Junhao WANG yunke zhu Xinyu zhu Hong QIN Ka BIAN Xianghai TANG Key Laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao 266003China

The economic alga Neopyropia,which holds significant value in China for its edibility and economic potential,harbors diverse epiphytic bacteria on its *** epiphytic bacteria on Neopyropia yezoensis and Neopyropia katadae,two nori species of Neopyropia living at the same tidal level but with distinct physiological states and living habits,were investigated to understand the differences between them and the effects of epiphytic bacteria on their differential *** of 16S rRNA gene sequences and real-time quantitative PCR(qPCR)of thalli and seawater samples(n=24)revealed notable differences in microbial diversity and community structure between the two *** dominated the bacterial communities in association with ***,whereas Proteobacteria were predominant in *** Investigation of Communities by Reconstruction of Unobserved States(PICRUSt2)analysis revealed enriched genes that related to the ABC transport system,iron complex outer membrane receptor proteins,and proliferation in ***,whereas *** exhibited enrichment of genes that related to energy supply as well as cell wall and cell membrane *** results of qPCR indicate a higher abundance of epiphytic bacteria on surface of *** than those on surface of *** findings indicate that differences in the living environments of *** and *** may result in distinct structural composition and abundance of epiphytic bacteria on their surfaces,thereby conferring specific biological functionalities to each microbial community,and the epiphytic bacteria may shape the living habits of hosts to some *** study provided a basis for understanding the close association between surface microorganisms and algal bodies,and the physiological and ecological characteristics of nori species.

关键词： Neopyropia yezoensis Neopyropia katadae epiphytic bacteria 16 S rRNA quantitative PCR(qPCR)

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Lateral quantum confinement regulates charge carrier transfer and biexciton interaction in CdSe/CdSeS core/crown nanoplatelets

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Nano Research 2023年第7期16卷 10420-10428页

作者： Yige Yao Xiaotian Bao yunke zhu Xinyu Sui An Hu Peng Bai Shufeng Wang Hong Yang Xinfeng Liu Yunan Gao State Key Laboratory for Artificial Microstructure and Mesoscopic Physics School of PhysicsPeking UniversityBeijing 100871China CAS Key Laboratory of Standardization and Measurement for Nanotechnology National Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Frontiers Science Center for Nano-optoelectronics Beijing 100871China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China Peking University Yangtze Delta Institute of Optoelectronics Nantong 226010China Dalian National Laboratory for Clean Energy Dalian 116023China

Charge carrier dynamics essentially determines the performance of various optoelectronic applications of colloidal semiconductor *** them,two-dimensional nanoplatelets provide new adjustment freedom for their unique core/crown ***,we demonstrate that by fine-tuning the core size and the lateral quantum confinement,the charge carrier transfer rate from the crown to the core can be varied by one order of magnitude in CdSe/CdSeS core/alloy-crown *** addition,the transfer can be affected by a carrier blocking mechanism,i.e.,the filled carriers hinder further possible ***,we found that the biexciton interaction is oppositely affected by quantum confinement and electron delocalization,resulting in a non-monotonic variation of the biexciton binding energy with the emission *** work provides new observations and insights into the charge carrier transfer dynamics and exciton interactions in colloidal nanoplatelets and will promote their further applications in lasing,display,sensing,etc.

关键词： nanoplatelets core/crown charge carrier transfer ultrafast spectroscopy biexciton

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Targeted transfer of self-assembled CdSe nanoplatelet film onto WS_(2) flakes to construct hybrid heterostructures

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Journal of Semiconductors 2021年第8期42卷 77-81页

作者： Zeguo Song Yunkun Wang yunke zhu Peng Bai An Hu Yunan Gao State Key Laboratory for Artificial Microstructure and Mesoscopic Physics School of PhysicsPeking UniversityBeijing 100871China Frontiers Science Center for Nano-optoelectronics Beijing 100871China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Colloidal CdSe nanoplatelets are thin semiconductor materials with atomic flatness surfaces and one-dimensional strong quantum confinement,and hence they own very narrow and anisotropic ***,we present a polydimethylsiloxane(PDMS)assisted transferring method that can pick up single layer CdSe nanoplatelet films self-assembled on a liquid surface and then precisely transfer to a *** layer-by-layer picking up and transferring,multiple layers of CdSe films can be built up to form CdSe stacks with each single layer having dominant in-plane transition dipole distribution,which both material and energic structures are analogous to traditional multiple quantum wells grown by molecular-beam ***,with the great flexibility of colloidal nanoplatelets and this transferring method,CdSe nanoplatelets films can be combined with other materials to form hybrid *** transferred a single-layer CdSe film onto WS_(2) flakes,and precisely studied the fast energy transfer rate with controlled CdSe nanoplatelet orientation and by using a streak camera with a ps time resolution.

关键词： CdSe nanoplatelets self-assembly transfer energy transfer hybrid heterostructures WS_(2)

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Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns in Pyropia yezoensis (Bangiales,Rhodophyta)

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Journal of Oceanology and Limnology 2021年第4期39卷 1447-1457页

作者： Lu WANG Junhao WANG yunke zhu Zhengcai CUI Fanna KONG Xianghai TANG Yunxiang MAO Key Laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao 266003China Key Laboratory of Utilization and Conservation of Tropical Marine Bioresource(Ministry of Education) College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya 572022China

The genus Pyropia contains several important cultivated *** research in nori species has mainly focused on the cell nucleus,with few studies on organelles(chloroplast and mitochondria).Due to the high copy numbers of organelles in cells,which influence the development and traits of algae,it is necessary to study their genetic *** this study,the marine red alga Pyropia yezoensis,an important economic macroalga,was selected as the study *** investigate organelle(chloroplast and mitochondria)inheritance in ***,the wild type RZ(maternal strain)was crossed with the red mutant HT(paternal strain)and 30 color-sectors from 11 F1 gametophytic blades were *** complete chloroplast and mitochondrial genomes of the red mutant(HT)were assembled for the first *** reliable and stable single nucleotide polymorphism(SNP)loci filtrated by bioinformatics analysis was used as a molecular marker for chloroplast and mitochondrial DNA,respectively,in subsequent *** amplification and sequence analysis showed that the haplotypes of color-sectors detected were consistent with those of the maternal parent,confirming that both chloroplast and mitochondrial genomes were inherited maternally in *** inheritance pattern of organelles in *** can be used to guide the hybridization and breeding of ***,the organelle SNP markers developed in this study can be applied in subsequent genetic research.

关键词： Pyropia yezoensis organelle single nucleotide polymorphism(SNP)markers chloroplast mitochondrial organelle inheritance maternal inheritance

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考虑电站调峰性能差异的短期精细化调度方法

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人民珠江 2024年第1期45卷 139-145页

作者：黄馗程瑞翔胡甲秋李向阳朱昀柯武新宇广西电网有限责任公司电力调度控制中心广西南宁530023 大连理工大学水电与水信息研究所辽宁大连116024 水利部珠江水利委员会珠江水利综合技术中心广东广州510611

随着电网调管水电规模的扩大以及调度精细化的需求,如何考虑不同电站调节性能差异进行精细化调峰,使得火电平稳运行,减少参与深度调峰,成为电网短期水电调度亟需解决的关键难题。针对电网短期精细化调度问题,提出了在水电站群调峰优化... 详细信息

随着电网调管水电规模的扩大以及调度精细化的需求,如何考虑不同电站调节性能差异进行精细化调峰,使得火电平稳运行,减少参与深度调峰,成为电网短期水电调度亟需解决的关键难题。针对电网短期精细化调度问题,提出了在水电站群调峰优化调度模型中考虑各水电站调峰性能差异的差异化调峰优化模型,更好地利用水电的调节能力,使得短期调度计划编制更加准确、合理。依照电网所辖水电站的调节性能不同划分弱调峰电站、调峰电站以及平衡电站进行差异化约束处理,并采用关联搜索方法对差异化调峰优化模型进行求解。选取广西37座水电站进行实例验证,结果表明:对比传统调峰优化模型,差异化调峰优化模型考虑了不同水电站调节性能的差异,进一步降低系统剩余负荷的峰谷差,平抑剩余系统负荷波动并调平系统负荷微小和频繁波动,是一种满足实际工程需求的精细化调度方法。

关键词：精细化调度差异化调峰短期调度梯级水电站群

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单向式胸腔镜基底段切除术352例的单中心回顾性研究

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中国胸心血管外科临床杂志 2022年第10期29卷 1284-1289页

作者：刘成武蒲强梅建东朱云柯马林郭成林刘伦旭四川大学华西医院胸外科成都610041

目的分享单向式胸腔镜解剖性基底段切除术的临床应用经验。方法回顾性分析2015年4月—2021年4月于四川大学华西医院行胸腔镜基底段切除术352例患者的临床资料,其中男96例、女256例,中位年龄50(26~81)岁。全组患者均通过胸腔镜手术,经... 详细信息

目的分享单向式胸腔镜解剖性基底段切除术的临床应用经验。方法回顾性分析2015年4月—2021年4月于四川大学华西医院行胸腔镜基底段切除术352例患者的临床资料,其中男96例、女256例,中位年龄50(26~81)岁。全组患者均通过胸腔镜手术,经叶间裂或肺下韧带入路,采用单向式思路结合“干-支法”完成各基底段解剖性切除。结果全组患者均顺利完成手术,其中单孔手术49例、两孔手术3例、三孔手术300例,无增加切口或中转开胸者,无中转为肺叶切除者。中位手术时间118(45~340)min,中位术中出血量20(5~500)mL,中位术后引流时间2(1~22)d,中位术后住院时间4(2~24)d。术后并发症包括肺部感染6例、术后漏气时间>5 d或胸腔引流时间>7 d 18例、脑梗死1例、其它2例,所有患者均经治疗后好转出院。全组无住院期间死亡。结论胸腔镜下采用单向式方法完成解剖性基底段切除术思路清晰,安全可行。

关键词：电视胸腔镜手术肺段切除肺癌单向式

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一种超紧凑封装的光/电转换阵列模块

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光通信技术 2023年第6期47卷 61-65页

作者：朱云柯陈少勇吕晓萌徐霄一周涛中国电子科技集团公司第二十九研究所成都610029 微波光子技术四川省重点实验室成都610029

针对传统微波光子产品通道数多、体积和功耗大、集成度低的问题,设计了一种基于微波光子混合集成封装技术的超紧凑封装光/电转换阵列模块,通过将光子器件、微波芯片、可调衰减芯片、电路系统封装入一个壳体内,可以在超紧凑空间内实现光... 详细信息

针对传统微波光子产品通道数多、体积和功耗大、集成度低的问题,设计了一种基于微波光子混合集成封装技术的超紧凑封装光/电转换阵列模块,通过将光子器件、微波芯片、可调衰减芯片、电路系统封装入一个壳体内,可以在超紧凑空间内实现光/电转换、信号放大、衰减可调和均衡等多项功能。测试结果表明:该模块在2~18 GHz频段光电响应度较高,大于0.8 A/W;6个通道的S21的平均值为-3.5 dB,每个通道的平坦度在±1.5 dB以内,通道隔离度大于55 dB,且驻波反射小于1.7。

关键词：微波光子混合集成封装光/电转换数控衰减光耦合

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肺鳞癌和肺腺癌胸内淋巴结转移相关因素分析

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中国肿瘤临床 2009年第20期36卷 1156-1159页

作者：马军亮刘伦旭蒲强朱云柯四川大学华西医院胸心外科成都市610041

目的:分析肺鳞癌和腺癌胸内淋巴转移的相关危险因素,为临床的分析判断提供依据。方法:对2006年6月至2008年6月本院行手术治疗140例肺鳞癌和腺癌患者的临床资料作回顾性分析。通过单因素x^2检验及多因素非条件Logistic回归统计分析,分析... 详细信息

目的:分析肺鳞癌和腺癌胸内淋巴转移的相关危险因素,为临床的分析判断提供依据。方法:对2006年6月至2008年6月本院行手术治疗140例肺鳞癌和腺癌患者的临床资料作回顾性分析。通过单因素x^2检验及多因素非条件Logistic回归统计分析,分析患者的性别、年龄、术前病程、肿瘤位置、肿瘤大小、病理类型、细胞分化程度、有无局部侵犯和T分期等相关因素与胸内淋巴结转移的相关关系。结果:在单因素分析中,胸内淋巴结转移与性别,肿瘤的位置、大小、细胞分化程度、局部侵犯、T分期有统计学意义(P女性(36.1%);中央型肺癌(65.3%)>周围型肺癌(45.1%);直径>3cm肿瘤(62.8%)直径≤3cm的肿瘤(35.2%);中分化以下的(59.8%)>中分化及以上的(37.5%);有外侵肿瘤(77.3%)大于没有外侵的肿瘤(47.5%);pT_4>pT_3>pT_2>pT_1(82.4%>66.6%>60%>20%)。而在多因素分析中,胸内淋巴结转移与T分期、肿瘤大小、局部外侵具有统计学意义(P<0.05)。结论:T分期、肿瘤大小、局部外侵是肺鳞癌及腺癌胸内淋巴结转移的危险因素。

关键词：肿瘤淋巴结转移 Logistic回归

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达芬奇机器人辅助与胸腹腔镜辅助微创食管癌切除术临床效果的系统评价与Meta分析

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中国胸心血管外科临床杂志 2022年第9期29卷 1181-1188页

作者：李刚张嘉妮沈旭阿来古哈徐智杰宋铁牛朱云柯林一丹四川大学华西医院胸外科成都610041 成都市公共卫生临床医疗中心成都610011 四川大学华西护理学院成都610011 四川大学华西临床医学院成都610011

目的系统评价达芬奇机器人辅助微创食管癌切除术(robot-assisted minimally invasive esophagectomy,RAMIE)和腔镜辅助微创食管癌切除术(video-assisted minimally invasive esophagectomy,VAMIE)的临床效果差异。方法计算机检索PubMed,EMbase、Medline、the Cochrane Library、CNKI数据库关于RAMIE和VAMIE临床效果对比的文献,检索时限为建库至2019年12月31日。由2名检索员独立筛选文献、提取资料。采用纽卡斯尔-渥太华量表评估文献质量,采用RevMan 5.3进行Meta分析。结果共14篇文献1160例患者纳入最终研究,其中12篇文献为高质量文献。RAMIE并不明显延长总手术时间(P=0.20);其中在McKeown手术中的胸部手术时间方面,RAMIE与VAMIE差异无统计学意义[MD=3.35,95%CI(–3.93,10.62),P=0.37];RAMIE与VAMIE术中出血量差异无统计学意义[MD=–9.48,95%CI(–27.91,8.95),P=0.31];RAMIE清扫淋巴结总数多于VAMIE[MD=2.24,95%CI(1.09,3.39),P=0.0001];RAMIE可清扫更多的左侧喉返神经旁淋巴结[MD=0.89,95%CI(0.13,1.65),P=0.02],降低术后声音嘶哑的发生率[RR=0.70,95%CI(0.53,0.92),P=0.009]。结论RAMIE与VAMIE在手术时间及术中出血量等方面差异无统计学意义。但RAMIE可清扫更多的淋巴结,尤其是左侧喉返神经旁淋巴结,减少清扫左侧喉返神经旁淋巴结时左侧喉返神经损伤的发生,从而降低术后声音嘶哑的发生率。

关键词：达芬奇机器人辅助系统胸腔镜手术微创食管癌切除术食管癌系统评价/Meta分析

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