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Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

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Journal of Integrative Plant Biology 2010年第10期52卷 891-903页

作者： Yuchul Jung Jungan Park yunjung choi Jin-Gweon Yang Donggiun Kim Beom-Gi Kim Kyunghee Roh Dong-Hee Lee Chung-Kyoon Auh Sukchan Lee Department of Genetic Engineering Sungkyunkwan University Institute of Life Science and Technology Sungkyunkwan University National Academy of Agricultural Science Rural Development Administration Department of Life Science Ewha Womans University Department of Biological Sciences Mokpo National University

Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCI. The expression of At-pyrroline-5-carboxylate synthetase was not affected by NaCI stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCI. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCI. Mannitol induced L-proline accumulation more rapidly than NaCI or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.

关键词： Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

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Adsorption of phosphate on magnetite-enriched particles (MEP) separated from the mill scale

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Frontiers of Environmental Science & Engineering 2019年第5期13卷 81-92页

作者： Muhammad Kashif Shahid yunjung Kim Young-Gyun choi Department of Environmental Engineering Chungnam National UniversityDaejeon 34134Republic of Korea Mechanical Process Research Group Engineering CenterPOSCO E&CIncheon 220099Republic of Korea

Phosphate is a major pollutant in water, causing serious environmental and health consequences. In present study, the phosphate adsorption on novel magnetite-enriched particles (MEP) was comprehensively investigated. A new method and device were introduced for the separation of MEP from the mill scale at low magnetic intensity. Particles were characterized with different techniques such as XRD, XRF, SEM and EDS. The XRD and XRF analysis of MEP identified the dominant existence of crystalline magnetite. Furthermore, the morphological analysis of MEP confirmed the agglomerate porous morphology of magnetite. Oxygen and iron, the main constituents of magnetite were acknowledged during the elemental analysis using EDS. The phosphate adsorption on MEP is well explained using various isotherm and kinetic models, exhibiting the monolayer adsorption of phosphate on the surface of MEP. The maximum adsorption capacity was determined 6.41 mg/g. Based on particle size (45–75 and 75–150 μm) and empty bed contact time (1 and 2 h), four columns were operated for 54 days. MEP were appeared successful to remove all phosphate concentration from the column influent having 2 mg/L concentration. The operated column reactors were successfully regenerated with alkaline solution. The results indicated potential for practical application of the MEP for phosphate removal.

关键词： Adsorption Magnetite Mill-scale Phosphate Wastewater treatment

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