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Click chemistry in the design of AIEgens for biosensing and bioimaging

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聚集体(英文) 2023年第4期4卷 71-92页

作者： Marie-Claire Giel yuning hong Department of Biochemistry and Chemistry La Trobe Institute for Molecular ScienceLa Trobe UniversityMelbourneVictoriaAustralia

The development of rapid,selective,and sensitivefluorescent sensors is essential for visualizing and quantifying biological molecules and processes in vitro,ex vitro,and in vivo,which is important for not only fundamental biological studies but the accurate diagnosis of *** emergingfield of activity-based sensing(ABS),a sensing method that utilizes molecular reactivity for analyte detection possesses many advantages including high specificity,sensitivity and *** aggrega-tion caused quenching phenomenon which occurs in most conventionalfluorophores results in reduced labeling efficiency of the target analytes and low photobleaching resistance,therefore limiting the applications of the ABS *** contrast,aggre-gation induced emission(AIE)active luminogens(AIEgens)provide exceptional molecular frameworks for *** the many reaction classes utilized in the AIEgen ABS approach,click chemistry has become increasing *** this review,the sensing concepts of the ABS approach with AIEgens and the principles of click chemistry are discussed,followed by a systematic summary of the application of specific click chemistry reactions in AIEgen ABS protocols for the detection of an array of target ***,the utility of click chemistry in the construction of AIEgens for bioimaging will also be showcased throughout the review.

关键词： activity-based sensing aggregation induced emission bioconjugation chemistry click chemistry

Production and emission of phosphine gas from wetland ecosystems

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Journal of Environmental Sciences 2010年第9期22卷 1309-1311页

作者： Chao Han Xueyuan Gu Jinju Geng yuning hong Rui Zhang Xiaorong Wang Shixiang Gao State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China School of Chemistry and Environmental Science Nanjing Normal University Nanjing 210097 China

Phosphine is a part of an atmospheric link of phosphorus cycle on earth, which could be an important pathway for phosphorus transport in environment. Wetland ecosystems are important locations for global biogeochemical phosphorus cycle. In this study, production and emission fluxes of free phosphine from four wetlands types in southern China were observed in different seasons. The results showed that the concentration of phosphine liberated from wetlands was at pg/m^3·ng/m^3 level. The emission concentrations of different wetlands followed the sequence： paddy field （51.83 ± 3.06） ng/m^3 〉/marsh （46.54 ± 20.55） ng/m^3 〉 lake （37.05 ± 22.74） ng/m^3 〉〉 coastal wetland （1.71 ± 0.73） ng/m^3, the positive phosphine emission flux occurred in rice paddy field （6.67 ± 5.18） ng/（m^***） and marsh （6.23 ± 26.9） ng/（m^***）, while a negative phosphine flux of （-13.11 ± 35.04） ng/（m^***） was observed on the water-air interface of Lake Taihu, suggesting that paddy field and marsh may be important sources for phosphine gas in atmosphere, while lake may be a sink of atmospheric phosphine gas during the sampling period. Atmospheric phosphine levels and emission flux from Yancheng marsh and rice paddy field varied in different seasons and vegetational zones. Both diffusion resistance in aqueous phase and temperature were dominating factors for the production and transportation of phosphine to atmosphere.

关键词： phosphine flux wetland ecosystems

Effects of La^(3+),Ce^(3+) on nitrogen removal in sequencing batch reactor

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Frontiers of Environmental Science & Engineering 2009年第3期3卷 369-374页

作者： Qing XIA Rui LIANG Yuxiang MAO yuning hong Lili DING hongqiang REN Mingyu ZHAO State Key Laboratory of Pollution Control and Resource Reuse School of EnvironmentNanjing UniversityNanjing 210093China China National Environmental Monitoring Center Beijing 100012China Jiangsu Jiangshan Pharmaceutical Co. Ltd.Jingjiang 214500China Grandway Technology International Co. Ltd.Beijing 100102China

Batch experiments were conducted to study the short-term biological effects of rare earth ions(La^(3+),Ce^(3+))and their mixture on the nitrogen removal in a sequencing batch reactor(SBR).The data showed that higher NH4+–N removal rate,total inorganic nitrogen removal efficiency,and denitrification efficiency were achieved at lower concentrations of rare earth elements(REEs)(<1 mg/L).In the first hour of the aeration stage of SBR,the presence of REEs increased the total inorganic nitrogen removal efficiency and NH4+–N removal effi-ciency by 15.7%and 10%–15%,*** the concentrations of REEs were higher than 1 mg/L,the total inorganic nitrogen removal efficiency decreased,and nitrate was found to accumulate in the *** the concentrations of REEs was up to 50.0 mg/L,the total inorganic nitrogen removal efficiency was less than 30%of the control efficiency with a high level of *** concentrations of REEs were found to accelerate the nitrogen conversion and removal in SBR.

关键词： rare earth La^(3+) Ce^(3+) inorganic nitrogen nitrogen removal sequencing batch reactor(SBR)

Red blood cell membrane-camouflaged nanoparticles loaded with AIEgen and Poly(I: C) for enhanced tumoral photodynamic-immunotherapy

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National Science Review 2021年第6期8卷 46-59页

作者： Jun Dai Meng Wu Quan Wang Siyang Ding Xiaoqi Dong Liru Xue Qingqing Zhu Jian Zhou Fan Xia Shixuan Wang yuning hong Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Engineering Research Center of Nano-Geomaterials of Ministry of Education Faculty of Materials Science and ChemistryChina University of Geosciences Department of Chemistry and Physics La Trobe Institute for Molecular ScienceLa Trobe University College of Material Chemistry and Chemical EngineeringHangzhou Normal University

Red blood cell(RBC)-mimicking nanoparticles(NPs) offer a promising platform for drug delivery because of their prolonged circulation time, reduced immunogenicity and specific targeting ability. Herein, we report the design and preparation of RBC membrane-bound NPs(M@AP), for tumoral photodynamic-immunotherapy. The M@AP is formed by self-assembly of the positively charged aggregation-induced emission luminogen(AIEgen)(named P2-PPh3) and the negatively charged polyinosinic : polycytidylic acid(Poly(I : C)), followed by RBC membrane encapsulation. P2-PPh3 is an AIE-active conjugated polyelectrolyte with additional photosensitizing ability for photodynamic therapy(PDT), while Poly(I : C) serves as an immune-stimulant to stimulate both tumor and immune cells to activate immunity, and thus reduces tumor cell viability. When applied in tumor-bearing mice, the M@AP NPs are enriched in both the tumor region as a result of an enhanced permeability and retention(EPR)effect, and the spleen because of the homing effect of the RBC-mimicking shell. Upon light irradiation,P2-PPh3 promotes strong ROS generation in tumor cells, inducing the release of tumor antigens(TA). The anti-tumor immunity is further enhanced by the presence of Poly(I : C) in M@AP. Thus, this strategy combines the PDT properties of the AIE-active polyelectrolyte and immunotherapy properties of Poly(I : C) to achieve synergistic activation of the immune system for anti-tumor activity, providing a novel strategy for tumor treatment.

关键词： biomimetic drug delivery system aggregation-induced emission Poly(I : C) immunotherapy photodynamic therapy

Molecular probe for measuring proteostasis decline in cells

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Molecular probe for measuring proteostasis decline in cells

Measuring Unfolded Protein Load and Proteostasis in Cells based on AIEgens

第十届全国化学生物学学术会议

作者： yuning hong Department of Chemistry and Physics La Trobe Institute for Molecular Science La Trobe University

Maintaining proteostasis is an essential housekeeping function for cell survival. It involves chaperones and degradative pathways to ensure proteins fold correctly and to remove those that are misfolded, damaged or aggregated. When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report a thiol-reactive aggregation-induced emission dye,TPE-MI, to measure cellular unfolded protein load[1]. We find that TPE-MI fluorescence is enhanced upon reaction with cellular proteomes under conditions promoting accumulation of unfolded proteins. TPE-MI reactivity can be used to track which proteins expose more cysteine residues under stress through proteomic analysis. We show that TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin treatment of the malaria parasites Plasmodium falciparum. TPE-MI therefore holds promise as a tool to probe proteostasis mechanisms in disease.

关键词： Proteostasis protein folding neurodegenerative disease fluorescence

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Measuring Unfolded Protein Load and Proteostasis in Cells ba...

The effect of tailing lipidation on the bioactivity of antimicrobial peptides and their aggregation tendency Special Issue:Emerging Investigators

中国化学会第十三届全国分析化学年会

作者： yuning hong （Y. hong） Department of Chemistry and Physics La Trobe University

＜正＞When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report that the dye, tetraphenylethene maleimide （TPE-MI） can be used to measure cellular unfolded protein load. TPE-MI fluorescence is activated upon labelling free cysteine thiols, normally buried in the core of globular proteins that are exposed upon unfolding. Crucially TPE-MI does not become fluorescent when conjugated to soluble glutathione. We find that TPE-MI fluorescence is enhanced upon reaction with cellular proteomes under conditions promoting accumulation of unfolded proteins.

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聚集体(英文) 2023年第4期4卷 195-209页

作者： Bruce Lin Andrew Hung William Singleton Kevion K.Darmawan Rachael Moses Bicheng Yao hongkang Wu Anders Barlow Marc-Antoine Sani Alastair J.Sloan Mohammed Akhter Hossain John D.Wade yuning hong Neil M.O’Brien-Simpson Wenyi Li The Bio21 Institute of Molecular Science and Biotechnology University of MelbourneMelbourneVictoriaAustralia ACTV Research group Melbourne Dental SchoolCentre for Oral Health ResearchUniversity of MelbourneMelbourneVictoriaAustralia School of Science RMIT UniversityMelbourneVictoriaAustralia Melbourne Dental School Division of Basic and Clinical Oral SciencesUniversity of MelbourneMelbourneVictoriaAustralia Department of Biochemistry and Chemistry La Trobe Institute for Molecular ScienceLa Trobe UniversityMelbourneVictoriaAustralia Florey Institute of Neuroscience and Mental Health University of MelbourneMelbourneVictoriaAustralia School of Chemistry University of MelbourneMelbourneVictoriaAustralia Materials Characterisation and Fabrication Platform University of MelbourneMelbourneVictoriaAustralia

Antimicrobial peptides(AMPs)are potentially powerful alternatives to conven-tional antibiotics in combating multidrug resistance,given their broad spectrum of *** mainly interact with cell membranes through surface electrostatic potentials and the formation of secondary structures,resulting in permeability and destruction of target microorganism *** earlier work showed that two leading AMPs,MSI-78(4–20)and pardaxin(1–22),had potent antimicrobial activ-ity against a range of *** is known that the attachment of moderate-length lipid carbon chains to cationic peptides can further improve the functionality of these peptides through enhanced interactions with the membrane lipid bilayer,inducing membrane curvature,destabilization,and potential ***,in this work,we aimed to investigate the antimicrobial activity,oligomerization propensity,and lipid-membrane binding interactions of a range of N-terminal lipidated analogs of MSI-78(4–20)and pardaxin(1–22).Molecular modeling results suggest that aggregation of the N-lipidated AMPs may impart greater structural stability to the peptides in solu-tion and a greater depth of lipid bilayer insertion for the N-lipidated AMPs over the parental *** experimental and computationalfindings provide insights into how N-terminal lipidation of AMPs may alter their conformations,with subsequent effects on their functional properties in regard to their self-aggregation behavior,membrane interactions,and antimicrobial activity.

关键词： aggregation antimicrobial peptide lipidation membrane active peptide

酚醛树脂/Ti3C2TxMXene导电复合材料制备与性能

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工程塑料应用 2020年第1期48卷 13-17页

作者：洪雨宁张翼吴波中电科芜湖钻石飞机制造有限公司安徽芜湖241000 南京工业大学南京210009

通过氢氟酸溶液刻蚀Ti3AlC2 MAX粉末制得Ti3C2TxMXene纳米片。然后,采用溶液共混的方法制备了酚醛树脂/Ti3C2TxMXene导电复合材料。通过X射线衍射、扫描电子显微镜等手段对其结构、微观形貌及性能进行表征。结果表明:Ti3C2TxMXene纳米... 详细信息

通过氢氟酸溶液刻蚀Ti3AlC2 MAX粉末制得Ti3C2TxMXene纳米片。然后,采用溶液共混的方法制备了酚醛树脂/Ti3C2TxMXene导电复合材料。通过X射线衍射、扫描电子显微镜等手段对其结构、微观形貌及性能进行表征。结果表明:Ti3C2TxMXene纳米片均匀分散在酚醛树脂里面,形成良好的导电通路。探讨Ti3C2TxMXene纳米片的用量对复合材料的导电性能和力学性能的影响。结果表明:酚醛树脂/Ti3C2TxMXene导电复合材料的电导率,冲击强度和拉伸强度随Ti3C2TxMXene纳米片含量的增加而逐渐增加;当Ti3C2TxMXene纳米片的含量为1.2%时,酚醛树脂/Ti3C2TxMXene导电复合材料的综合性能最优,此时酚醛树脂/Ti3C2TxMXene导电复合材料的电导率为4.36×104 S/m,冲击强度和弯曲强度分别为23.9 kJ/m2和65.9 MPa。

关键词： Ti3C2Tx MXene 酚醛树脂电导率复合材料

镧、铈对厌氧颗粒污泥产甲烷的Hormesis效应及其动力学研究

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环境科学学报 2007年第8期27卷 1233-1237页

作者：夏青任洪强丁丽丽洪宇宁吴吉春南京大学环境学院污染控制与资源化研究国家重点实验室南京210093 南京大学地球科学系南京210093

间歇培养条件下研究了单一稀土元素镧（La^3＋）和铈（Ce^3＋）在不同浓度水平下对厌氧颗粒污泥活性及其动力学行为的影响.结果表明,La^3＋和Ce^3＋对厌氧颗粒污泥比产甲烷活性有促进作用的浓度范围均为0.01～0.1 mg·L^-1,促进作用浓... 详细信息

间歇培养条件下研究了单一稀土元素镧（La^3＋）和铈（Ce^3＋）在不同浓度水平下对厌氧颗粒污泥活性及其动力学行为的影响.结果表明,La^3＋和Ce^3＋对厌氧颗粒污泥比产甲烷活性有促进作用的浓度范围均为0.01～0.1 mg·L^-1,促进作用浓度峰值均为0.05mg·L^-1,最大促进百分比分别为10.35%和20.79%;La^3＋和Ce^3＋对SMA（比甲烷活性）的半抑制浓度分别为5mg·L^-1和1000mg·L^-1,La^3＋较易对污泥产生抑制作用.米-门方程可以用来描述厌氧消化过程中甲烷发酵阶段的动力学行为,稀土元素La^3＋和Ce^3＋投加至反应系统后,提高了甲烷发酵过程的动力学常数Vmax和半饱和常数Ks.

关键词：镧铈稀土元素厌氧颗粒污泥产甲烷活性动力学