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第十二届全国植物基因组学大会

作者： yunhe jiang,liang bao,caiguo xu,xianghua li,qifa zhang~* national key laboratory of crop genetic improvement and national center of plant gene research,huazhong agricultural university,wuhan 430070,china

Organ size is an important component for yield and also an excellent model to address the basic biological question like cell *** growth is determined by the coordination of cell division and expansion. Although several plant hormones are known to play critical roles in shaping organ size by regulating cell cycle, it is not clear whether brassinosteroids(BRs) are also involved in regulating cell *** we identified a rice T-DNA insertion mutant for organ size,referred to as xiao displaying dwarfism and erect leaves,

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OsELF3 is Involved in Flowering Time Control Under Long-day Condition in Rice

OsELF3 is Involved in Flowering Time Control Under Long-day ...

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第十二届全国植物基因组学大会

作者： Ying Yang,Guoxing Chen,xianghua li,qifa zhang,Changyin Wu~* national key laboratory of crop genetic improvement and national center of plant gene research,wuhan 430070,china

Heading date is a critical trait which determining cropping seasons and regional adaptability in *** during the last decade have identified some conserved genes involved in photoperiod pathway between Arabidopsis and ***,some heading date genes,such as Ehd1,Ghd7 and RID1/Ehd2/ OsID1 are regarded as particular components belonging to *** this study,a late-flowering mutant was identified from rice Tos17-tagging ***17 has been inserted in the second intron of Oryza sativa ELF3(OsELF3), which shares 75%amino acid identity with the Arabidopsis ELF3 *** oself3 plants exhibit de-

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Overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice

Overexpressed glutamine synthetase gene modifies nitrogen me...

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第十一届全国植物基因组学大会

作者： Hong Mei,Caiying Zhou,Jinghua Xiao,xianghua li,qifa zhang Xingming lian~* national key laboratory of crop genetic improvement and national center of plant gene research(wuhan), huazhong agricultural university,wuhan 430070,china

Nitrogen is a crucial macronutrient that is essential and rate-limiting for the growth and development of *** synthetase(GS;EC 6.***.1.2) is a key enzyme in nitrogen metabolism;it catalyzes the critical incorporation of...

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OsELF3 is involved in flowering time control under long-day condition in rice

OsELF3 is involved in flowering time control under long-day ...

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第十一届全国植物基因组学大会

作者： Ying Yang,Guoxing Chen,xianghua li,qifa zhang,Changyin Wu~* national key laboratory of crop genetic improvement and national center of plant gene research(wuhan), huazhong agricultural university,wuhan 430070,P.R.china

Heading date is a critical trait which determining cropping seasons and regional adaptability in rice. Efforts during the last decade have identified some conserved genes involved in photoperiod pathway between Arabid...

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Overexpressing SNAC1, a transcription factor predominantly induced in guard cells, enhances drought resistance and salt tolerance in rice

Overexpressing SNAC1, a transcription factor predominantly i...

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湖北省遗传学会、江西省遗传学会2006年学术年会暨学术讨论会

作者： Honghong Hu, Mingqiu Dai, Jialing Yao, Benze Xiao, xianghua li, qifa zhang and lizhong Xiong national key laboratory of crop genetic improvement, national center of plant gene research (wuhan), huazhong agricultural university, wuhan, 430070, china

Drought and salinity are major abiotic stresses to crop production. Here a stress responsive gene SNACl encoding a NAC transcription factor is characterized for its role in stress resistance in rice. In leaves, this gene is induced specifically in guard cells by dehydration stress. The SNACl protein contains a nuclear localization signal located in the N-terminus that can sufficiently mediate nuclear targeting of the protein, a DNA binding domain in the middle that can bind to the NAC recognized sequence, and a C-terminus transactivation domain with 241-271 amino acids indispensable for the activation activity. Transgenic rice overexpressing the SNACl gene showed significantly enhanced drought resistance (22%-34% higher seed setting than control) in the field under severe drought stress conditions at the reproductive stage, while showing no phenotypic changes or yield penalty. The transgenic rice lost water more slowly by closing more stomatal pores and maintained turgor pressure at significantly lower level of relative water content than the wild type. The transgenic rice also shows significantly improved drought and salt tolerance (80% higher survival rate than control) at the vegetative stages. DNA chip analysis revealed that a large number of stress related genes including the rice homologue of early responsive to drought 1 (ERD1) were up-regulated in the SNAC1 -overexpressing rice plants. Our data suggest that SNACl holds promising utility in improving drought and salinity tolerance in rice.

关键词： Overexpressing SNAC1 a transcription factor predominantly induced in guard cells enhances drought resistance and salt tolerance in rice

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A global analysis of QTLs for expression variations in rice shoot at early seedling stage

A global analysis of QTLs for expression variations in rice ...

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第十一届全国植物基因组学大会

作者： Jia Wang~#,Huihui Yu~#,Weibo Xie,Yongzhong Xing,Sibin Yu,caiguo xu,xianghua li,Jinghua Xiao,qifa zhang~* national key laboratory of crop genetic improvement,national center of plant gene research,huazhong agricultural university,wuhan 430070,P.R.china

Analyses of QTLs for expression levels(eQTLs) of the genes reveal genetic relationship between expression variation and the regulator,thus unlocking the information for identifying the regulatory ***-nucleotide expres...

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T-DNA Integration into Rice Genome Depends on Sequences of Insertion Sites

T-DNA Integration into Rice Genome Depends on Sequences of I...

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第十二届全国植物基因组学大会

作者： Tuan Long~*,Ying Yang,Chengjun zhang,Xiaobo Zhao,Weibo Xie,Guoxing Chen,xianghua li,qifa zhang,Changyin Wu national key laboratory of crop genetic improvement and national center of plant gene research(wuhan),huazhong agricultural university,wuhan 430070,china

T-DNA insertional mutant library is a powerful platform for the research of plant functional genomics. Via isolating flanking sequence tag(FST) of the T-DNA insertion,the insertion site in the host genome can be easily tagged to bridge the genotype and the ***,the insertion site also implicates the mechanism of T-DNA integration,which is important to construct saturated mutant *** reveal the

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In depth exploration of flanking sequence tags in a rice T-DNA insertional library

In depth exploration of flanking sequence tags in a rice T-D...

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第十一届全国植物基因组学大会

作者： Tuan Long,Ying Yang,Chengjun zhang,Xiaobo Zhao,Guoxing Chen,xianghua li,qifa zhang,Changyin Wu~* national key laboratory of crop genetic improvement and national center of plant gene research(wuhan), huazhong agricultural university,wuhan 430070,P.R.china

An essential tool for the functional analysis of the completely sequenced rice genome is the generation of loss-of-function mutations for all of the *** the last decade,we have generated more than 120 thousand indepen...

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Comparative analysis of gene expression at early seedling stage between a rice hybrid and its parents using a cDNA microarray of 9198 uni-sequences

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Science china(life Sciences) 2006年第6期49卷 519-529页

作者： HUANG Yi li lihua CHEN Ying li xianghua xu caiguo WANG Shiping zhang qifa national key laboratory of crop genetic improvement National Center of Plant Gene Research WuhanHuazhong Agricultural UniversityWuhan 430070China

Using a cDNA microarray consisting of 9198 expressed sequence tags,we surveyed the gene expression profiles in shoots and roots of a rice hybrid,liangyoupei 9 and its parents Peiai 64s and 93-11 at 72 h after germination.A total of 8587 sequences had detectable signals in both shoots and roots of the three genotypes.A total of 1571 sequences exhibited significant(Plipid metabolisms and stress response than expected,while less frequently in categories of amino acid metabolism,transcription and translation regulation,and signal transduction.A total of 214 sequences exhibited significant(Pgenes with known functions,assigned in the categories of basic metabolism,genetic information processing,cell growth and death,signal transduction,transportation and stress *** results may provide useful information for exploring the relationship between gene expression polymorphism and phenotypic variation,and for characterizing the molecular mechanism of seedling development and heterosis in rice.

关键词： Oryza sativa shoots roots gene expression microarray heterosis

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Transcriptomic analysis of rice responses to low phosphorus stress

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Chinese Science Bulletin 2010年第3期55卷 251-258页

作者： li liHua QIU xuHua li xianghua WANG ShiPing zhang qifa liAN XingMing national key laboratory of crop genetic improvement. national center of plant gene research (wuhan) Huazhong Agricultural UniversityWuhan 430070 China

Phosphorus is one of the essential macronutrients required for plant growth and development. The plants increase the absorption of phosphorus through an expansion of the root system in the soil when they are in low-phosphorus conditions. At the same time, the changes in biochemical metabolic pathways and the increase of secretion of phosphatase and organic acids activate the insoluble phosphate fixed in the soil. The expression profile in response to low phosphorus was investigated for rice at 6, 24 and 72 h after low-phosphorus stress compared with normal phosphorus conditions as a control with DNA chip. A total of 1207 differen- tially expressed genes were found in our study; 795 and 450 genes exhibited alterations in their RNA expression in response to inorganic phosphate (Pi) starvation in at least one of the three time points in roots and shoots. Thirty-eight genes overlapped in shoots and roots. The functional classification of these genes indicated their involvement in various metabolic pathways, ion transport, signal transduction, transcriptional regulation, and other processes related to growth and development. A large number of transposable elements changed the expression in rice after low-phosphorus stress. The results may provide useful information about molecular processes associated with Pi deficiency and facilitate the identification of key molecular determinants for improving Pi use by crop species.

关键词：低磷胁迫转录调控胁迫反应水稻差异表达基因植物生长发育代谢途径 DNA芯片

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