检索结果-南通市图书馆

Photoinduced Carbene for Effective Photodynamic Therapy Against Hypoxic Cancer Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CCS Chemistry 2023年第9期5卷 2078-2087页

作者： Zhanghua Sun yuncong chen Shankun Yao Hao Yuan Dongfan Song Zijian Guo Weijiang He State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringChemistry and Biomedicine Innovation Center(ChemBIC)Nanjing UniversityNanjing 210023Jiangsu Nanchuang(Jiangsu)Institute of Chemistry and Health Nanjing 210000Jiangsu

Photodynamic therapy(PDT)has attracted much attention because of its advantages over chemotherapy,such as broad spectrum,high selectivity,and low drug resistance.However,most photosensitizers(PSs)used in PDT are O_(2)-dependent and show limited therapeutic efficacy toward hypoxic solid tumors.Therefore,developing PSs that produce reactive oxygen species(ROS)in an O_(2)-independent manner is highly demanded.Herein,we constructed a novel O_(2)-independent PS(TPA-N)based onα-diazo-aryl acetate,which could generate reactive carbene under visible light irradiation.Photoinduced carbene could react with biosubstrates and cause significant carbene stress and ROS,effectively killing tumor cells even under hypoxic conditions.TPA-N showed much less O_(2)dependence compared with traditional PDT reagent protoporphyrin IX.A mechanistic study suggested that TPAN could induce mitochondrial membrane potential collapse and upregulate apoptosis-related proteins upon light irradiation.This work provided a reliable strategy for developing O_(2)-independent PSs against hypoxic tumor cells through photoinduced carbene.

关键词： carbene apoptosis hypoxic photodynamic therapy tumor cells

Type I Photoreaction and Photoinduced Ferroptosis by a Ru(II)Complex to Overcome Tumor Hypoxia in Photodynamic Therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CCS Chemistry 2023年第7期5卷 1583-1591页

作者： Fen Qi Hao Yuan yuncong chen Xin-Xin Peng Yanping Wu Weijiang He Zijian Guo State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringChemistry and Biomedicine Innovation Center(ChemBIC)Nanjing UniversityNanjing 210023Jiangsu Nanchuang(Jiangsu)Institute of Chemistry and Health Nanjing 210000Jiangsu

Photodynamic therapy(PDT)usually shows limited efficacy in solid tumors since traditional PDT is O_(2)^(-)dependent while solid tumors are inherently hypoxic.In addition,hypoxic tumor cells possess antiapoptotic pathways that resist PDT-induced apoptosis.Therefore,developing photosensitizers(PSs)that show low O_(2)^(-)dependency and can induce nonapoptotic cell death pathways is critically needed.Herein,a series of Ru(II)polypyridine complex-based PSs,RuNMe,RuH,and RuCN,were synthesized,and their applications against hypoxic tumor cells through PDT were investigated.All three complexes showthe ability to generate the superoxide anion radical(·O_(2)^(-)),which is the type I photoreaction and less O_(2)^(-)dependent.RuNMe shows the best PDT performance against MCF-7 cells and three-dimensional multicellular spheroids,due to its higher cellular uptake and more reactive oxygen species generation.More importantly,RuNMe-incubated MCF-7 cells show photoinduced ferroptosis as evidenced by glutathione peroxidase 4 downregulation and lipid peroxide accumulation.This work not only develops a novel ferroptosis-inducing Ru(II)complex with the type I PDT process but also offers an effective strategy to solve tumor hypoxia in PDT.

关键词： ferroptosis photodynamic therapy O2-independent type I photoreaction superoxide anion radical(·O_(2)^(-))

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

回鹘文《慈悲道场忏法》音译词语音系统研究

敦煌学辑刊 2024年第2期2卷 124-134页

作者：陈云聪张铁山中央民族大学中国少数民族语言文学学院北京100081

回鹘文《慈悲道场忏法》译自汉文本,是现存回鹘文文献中篇幅较大、内容丰富的佛教文献之一.以Jens Wilkens的研究成果为底本,可以梳理出其中的音译汉语词,进而研究回鹘汉对音,为进一步认识汉语语音的音值和音变提供例证.回鹘译音反映的... 详细信息

回鹘文《慈悲道场忏法》译自汉文本,是现存回鹘文文献中篇幅较大、内容丰富的佛教文献之一.以Jens Wilkens的研究成果为底本,可以梳理出其中的音译汉语词,进而研究回鹘汉对音,为进一步认识汉语语音的音值和音变提供例证.回鹘译音反映的日母鼻音成分的失落、阳声韵尾的弱化等,是唐宋西北方音的延续和发展;见组对音分为两类、日母对y、韵母单元音化等则显示了回鹘语语音系统对汉语的影响.

关键词：回鹘文《慈悲道场忏法》回鹘汉对音西北方音

Polarity-triggered anti-Kasha system for high-contrast cell imaging and classification

在线全文

学校读者我要写书评

暂无评论

聚集体(英文)

聚集体(英文) 2023年第2期4卷 216-226页

作者： Junyi Gong Peifa Wei Junkai Liu yuncong chen Zheng Zhao Weijun Zhao Huilin Xie Chao Ma Jacky W.Y.Lam Kam Sing Wong Ying Li Ben Zhong Tang Shenzhen Institute of Aggregate Science and Technology School of Science and EngineeringThe Chinese University of Hong KongShenzhenChina Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced StudyState Key Laboratory of Molecular NanoscienceDivision of Life Science and Department of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyClear Water BayKowloonChina Institutes of Physical Science and Information Technology Anhui UniversityHefeiChina Department of Physics HKUSTKowloonChina Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology The NMPA and State Key Laboratory of Respiratory DiseaseInnovation Research Center for AIE Pharmaceutical BiologySchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhouChina Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Material Science and EngineeringShenzhen UniversityShenzhenChina Center for Aggregation-Induced Emission SCUT-HKUST Joint Research InstituteState Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhouChina AIE Institute Guangzhou Development DistrictHuangpuChina

Kasha’s rule,which states that all exciton emissions occur from the lowest excited state and are independent of excitation energy,makes high-energy excitons difficult to use and severely hinders the widespread applications of organic photoluminescent materials in the real world.For decades,scientists have tried to break this rule to unleash the power of high-energy excitons,but only minimal progress has been achieved,with no rational guiding principles provided,and few applications developed.So far,breaking Kasha’s rule has remained a purely academic concept.In this paper,we introduce a design principle for a purely organic anti-Kasha system and synthesise a series of compounds based on the design rule.As predicted,these compounds all display evident S_(2) emissions in dilute solutions.In addition,we introduce a highly accurate(over 90%)convolutional neural network as an assistant for the classification of cells using anti-Kasha luminogens,thereby providing a new application direction for anti-Kasha systems.

关键词： anti-Kasha cell classification excited-state dynamics fluorescence

BODIPY-derived ratiometric fluorescent sensors: pH-regulated aggregation-induced emission and imaging application in cellular acidification triggered by crystalline silica exposure

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2018年第11期61卷 1413-1422页

作者： Yang Bai Danyang Liu Zhong Han yuncong chen Zhongyan chen Yang Jiao Weijiang He Zijian Guo State Key Laboratory of Coordination Chemistry Coordination Chemistry Institute School of Chemistry and Chemical EngineeringNanjing University

Modification of classic fluorophore to possess the emission transitions between aggregation-induced emission(AIE)and intrinsic emission offers reliable approach to the design of ratiometric fluorescent sensors.In this study,a proton acceptor benzimidazole was integrated with BODIPY to form three compounds,BBI-1/2/3,which demonstrated the AIE(~595 nm,I_(agg))in neutral aqueous medium and intrinsic BODIPYemission(~510 nm,I_(int))in acidic medium.All the three showed the ratiometric pH sensing behavior in a dual excitation/dual emission mode,yet BBI-3 displayed still the dual emission ratiometric pH sensing ability.The pH-dependent emission ratio I_(int)/I_(agg)of the three were fully reversible,and no interference was observed from normal abundant chemical species in live cells.Their different p K_a(BBI-1,p K_a4.4;BBI-2,p K_a2.7;BBI-3,p K_a3.6)suggested that the substituents on benzimidazole moiety were essential to govern their functioning pH range.The ratiometric imaging of BBI-1 in A549 cells provided an effective intracellular pH(pH_i)calibration formula corresponding to emission ratio of I_(int)/I_(agg).Ratiometric pH_iimaging in A549 cells upon small particle exposure confirmed the particle-induced cellular acidification with this formula.Both particle size and the chemical nature of the particle contribute to the observed acidification effect.The synchronization of lysosome disruption to cellular acidification in A549 cells upon crystalline silica exposure was directly observed for the first time with BBI-1,showing the potential application of BBI-1 in the study of silicosis and other related diseases.This study demonstrated that endowing fluorophore with AIE/intrinsic emission transition could be a promising strategy for ratiometric sensor design.

关键词：传感器设计细胞内荧光灯排放 pH 发酸水晶成像

A dual-modal probe for NIR fluorogenic and ratiometric photoacoustic imaging of Cys/Hcy in vivo

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2020年第5期63卷 699-706页

作者： Hongbao Fang yuncong chen Yanjun Wang Shanshan Geng Shankun Yao Dongfan Song Weijiang He Zijian Guo State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Chemistry and Biomedicine Innovation Center Nanjing UniversityNanjing 210023China

Biothiols, such as cysteine(Cys) and homocysteine(Hcy), play vital roles in biological homeostasis and are closely related to various pathological and physiological processes in the living systems. Therefore, the in vivo detection of biothiols is of great importance for early diagnosis of diseases and assessment of disease progression. In this work, we developed a near-infrared(NIR) fluorescence and photoacoustic dual-modal molecular probe(NIR-S) that can be specifically activated by Cys or Hcy. The aryl-thioether substituted cyanine probe can undergo nucleophilic substitution and Smiles rearrangement reaction, resulting in specific turn-on NIR fluorescence and ratiometric photoacoustic responses for Hcy/Cys. Thus, NIR-S not only realizes the specific NIR fluorescence and photoacoustic dual mode imaging to detect Hcy/Cys in solution, but also can be applied to living cells and mice to detect Hcy/Cys. This work provided a practical tool to detect Hcy/Cys levels in vivo, which would be beneficial for the early diagnosis and progress of diseases.

关键词： Cys/Hcy NIR fluorescence imaging ratiometric PA imaging dual-modality in vivo

A ratiometric fluorescent sensor for tracking Cu(Ⅰ) fluctuation in endoplasmic reticulum

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2019年第4期62卷 465-474页

作者： Jungu Guo Hao Yuan yuncong chen Zhongyan chen Menglong Zhao Liang Zou Yi Liu Zhipeng Liu Qiang Zhao Zijian Guo Weijiang He State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing University Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)Nanjing University of Posts & Telecommunications Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University (NanjingTech)

A two-photon ratiometric fluorescent sensor for Cu^+ in endoplasmic reticulum(ER), CNSB, was developed via coumarin/ASBD integration based on FRET mechanism. In solution, CNSB shows reversible, highly-specific ratiometric response to Cu^+ .Moreover, CNSB exhibits suitable K_d value, suggesting the possibility of detecting Cu^+ in the living cells. The probe can enter the MCF-7 cells easily and specifically locates in the ER. The highly specific ratiometric response of CNSB toward Cu^+ in MCF-7 cells provides the sensor the capacity to visualize both exogenous and endogenous Cu^+ in the ER via fluorescence imaging.Next, CNSB was utilized to detect the fluctuation and distribution of Cu^+ under ER stress in MCF-7 cells, which confirmed directly the relationship between Cu^+ enhancement and ER stress. Meanwhile, the two-photon ability of coumarin facilitated the sensor to visualize Cu^+ fluctuation via two-photon fluorescence imaging. In addition, the spatial distribution of Cu^+ in the heart slice of the 14-day-old rat was demonstrated using CNSB. This study demonstrates the promising potential of CNSB in clarifying the Cu^+ -dependent signaling in the ER stress-related diseases.

关键词： Cu(Ⅰ) ratiometric two-photon endoplasmic reticulum-targeting endoplasmic reticulum stress heart tissue slice

Recent endeavors on molecular imaging for mapping metals in biology

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2020年第5期6卷 159-178页

作者： Jing Gao yuncong chen Zijian Guo Weijiang He State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Nanjing University Chemistry and Biomedicine Innovation Center Nanjing University

Transition metals such as zinc, copper and iron play vital roles in maintaining physiological functions and homeostasis of living systems. Molecular imaging including two-photon imaging(TPI), bioluminescence imaging(BLI) and photoacoustic imaging(PAI), could act as non-invasive toolkits for capturing dynamic events in living cells, tissues and whole animals. Herein, we review the recent progress in the development of molecular probes for essential transition metals and their biological applications.We emphasize the contributions of metallostasis to health and disease, and discuss the future research directions about how to harness the great potential of metal sensors.

关键词： Molecular imaging Zinc Copper Iron

新型冠状病毒感染疫情常态化防控背景下社区医务人员心理健康状况及影响因素分析

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国全科医学 2024年第7期27卷 834-842页

作者：杨华杰王皓翔陈运聪梁旭东王家骥黄翔广东开放大学(广东理工职业学院)健康产业学院广东省中山市528400 中山大学公共卫生学院广东省广州市510080 广东省中山市三乡镇社区卫生服务中心公共卫生业务管理科 528463 广东省中山市板芙镇社区卫生服务中心 528459 广东省广州市广东省基层卫生协会510000 澳门科技大学医学院中国澳门特别行政区999078

背景自新型冠状病毒感染(简称新冠感染)疫情发生以来,身处防控一线的社区医务人员面临着直接接触病毒的风险,存在工作强度大、工作时间长、心理压力大等问题,因此探究社区医务人员的心理健康状况及其内在联系对精准实施心理干预具有重... 详细信息

背景自新型冠状病毒感染(简称新冠感染)疫情发生以来,身处防控一线的社区医务人员面临着直接接触病毒的风险,存在工作强度大、工作时间长、心理压力大等问题,因此探究社区医务人员的心理健康状况及其内在联系对精准实施心理干预具有重要意义。目的了解新冠感染疫情常态化防控期间社区医务人员心理健康状况并探讨其影响因素。方法于2021年12月,采用多阶段随机抽样法,选取296名中山市社区医务人员作为调查对象,采用一般资料调查表、自行编制的社区医务人员新型冠状病毒感染疫情防控工作压力调查问卷、广泛性焦虑量表(GAD-7)和患者健康问卷抑郁症状群量表(PHQ-9)对其进行网络问卷调查,采用多因素Logistic回归分析探讨社区医务人员新冠感染疫情防控工作感知压力水平及焦虑、抑郁情绪状态存在情况的影响因素。结果中山市社区医务人员新冠感染疫情防控工作高感知压力的检出率为10.1%(30/296);中度及以上焦虑和抑郁的检出率分别为7.1%(21/296)和9.5%(28/296);性别、抑郁情绪状态存在情况和新冠感染疫情防控工作感知压力水平是社区医务人员焦虑情绪状态存在情况的影响因素(P<0.05);疫情期间从事日常诊疗工作情况、疫情期间从事“三人小组”上门排查工作情况、焦虑情绪状态存在情况和新冠感染疫情防控工作感知压力水平是社区医务人员抑郁情绪状态存在情况的影响因素(P<0.05);焦虑、抑郁情绪状态存在情况是社区医务人员新冠感染疫情防控工作感知压力水平的影响因素(P<0.05)。结论社区医务人员新冠感染疫情防控工作压力与焦虑和抑郁负性情绪之间彼此关联、相互影响、相互作用,且疫情防控工作任务与人力资源的相对不足等因素加剧了社区医务人员负性情绪的叠加效应,卫生健康行政部门应结合疫情常态化防控阶段社区卫生服务机构的发展特点及暴露出的突出问题,着力构建新形势下社区卫生服务机构的长效投入和发展机制,加强对医务人员的心理干预指导。

关键词：新型冠状病毒感染社区卫生工作者社区医务人员心理健康影响因素分析