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The neuroprotective effect of mesenchymal stem cells is mediated through inhibition of apoptosis in hypoxic ischemic injury

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World Journal of Pediatrics 2020年第2期16卷 193-200页

作者： Fang Li Kun Zhang Hua Liu Tan Yang Dong-Jie Xiao yun-shan wang Laboratory of Cell Therapy and Translational Medicine Jinan Central Hospital Affiliated to Shandong University105 Jiefang RoadJinan 250013China shandong Research Center of Transplantation and Tissue Jinan 250013China Clinical Laboratory The People's Hospital of BozhouBozhou 236800China

Background Neonatal hypoxia ischemia causes severe brain *** cell therapy is a promising method for treating neuronal *** translation of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)for the recovery of neurons after hypoxic ischemic encephalopathy(HIE)may represent an effective *** Primary neurons were exposed to oxygen-glucose deprivation(OGD)and subsequently cocultured with *** was examined by Annexin *** related to apoptosis were detected using RT-PCR and westernblot *** an in vivo model,HIE was induced in postnatal day 7 mice,and UC-MSCs were transplanted via the intraventricular ***-MSC migration was investigated by immunofluorescence,and lesion volumes were measured by TTC *** in injured brain cells was detected by the TUNEL ***-PCR and ELISA were used to detect the expression of inflammatory factors in cells and animal *** Flow cytometry analysis revealed that apoptosis in injured neurons was inhibited by *** RT-PCR and western blot results indicated that coculture inhibited the expression of proapoptotic genes and upregulated expression of antiapoptotic *** the animal model,transplanted UC-MSCs migrated toward the cerebral lesion site and decreased the lesion extent in *** staining showed that the MSC group exhibited significantly reduced numbers of TUNEL-positive ***-PCR and ELISA showed that UC-MSCs inhibited the upregulation of TNF-αand IL-1βin response to hypoxic ischemic *** These results indicate that UC-MSCs exert neuroprotective effects against hypoxic ischemic injury by inhibiting apoptosis,and the mechanism appears to be through alleviating the inflammatory response.

关键词： Apoptosis Hypoxic ischemic encephalopathy Mesenchymal stem cell Transplantation

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Male reproductive function before and after the adjustment of the COVID-19 prevention policy:a multicenter study in China

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Asian Journal of Andrology 2024年第3期26卷 308-314页

作者： Jian-Zheng Li Han Wu Li wang yun-shan wang Feng Kong Long-Qiao Cao Ming Liang Xiong wang Ming-Zhen Yuan shandong Provincial Hospital Affiliated to shandong First Medical University Jinan 250000China Institute of Women Children and Reproductive HealthShandong UniversityJinan 250012China Engineering Laboratory of Urinary Organ and Functional Reconstruction of shandong Province Jinan 250000China The First People’sHospital of Jining Jining 272000China Department of Reproductive Medicine The Second Affiliated Hospital of Shandong University of Traditional Chinese MedicineJinan 250000China Reproductive Medicine Center Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityYantai 264000China

At the end of 2022,the adjustment of the coronavirus disease 2019(COVID-19)pandemic control policy in China resulted in alarge-scale increase in public *** compare the fertility parameters of male patients before and after the adjustments ofthe COVID-19 pandemic control policy in China,we collected data on patients’medical histories and laboratory examinations ontheir first visits between June 2022 and March 2023 in five different *** were divided into five groups according tothe timeline of the policy *** data we collected from male patients included semen quality and serum reproductivehormone levels,and intergroup comparisons were made using the Mann–Whitney U and Chi-square *** total,16784 casesunderwent regular semen analysis,11180 had sperm morphology assessments,and 7200 had reproductive hormone *** showed declining trends in semen volume,sperm motility,and the progressive sperm motility rate after the policy *** comparison revealed an initial decrease and gradual recovery in progressive motility *** morphology analysisshowed increased neck and tail abnormalities after the policy *** significant change in hormone levels was *** the adjustment of the COVID-19 prevention policy in China,a decline in sperm motility and morphology was *** trend may gradually recover over 2 *** the policy adjustment,reproductive hormone levels were relatively stablethroughout,except for an increase in luteinizing hormone(LH).These changes in semen parameters suggest that the policyadjustment had a short-to medium-term impact on male reproductive function.

关键词： COVID-19 multicenter study reproduction SARS-CoV-2 semen analysis

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Upper Large Deviations for Mixing Random Sequence

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Communications in Mathematical Research 2010年第3期26卷 219-229页

作者： ZHANG YONG YANG XIAO-yun DONG ZHI-shan wang De-hui College of Mathematics Jilin University Changchun 130012

In this article, we prove upper large deviations for the empirical measure generated by stationary mixing random sequence under some suitable assumptions and upper large deviations for the mixing random sequence.

关键词： mixing sequence empirical measure large deviation

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DEC1 nuclear expression:A marker of differentiation grade in hepatocellular carcinoma

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World Journal of Gastroenterology 2011年第15期17卷 2037-2043页

作者： Xiao-Hong Shi Yan Zheng Qing Sun Jing Cui Qing-Hua Liu Fei Qü yun-shan wang Department of Laboratory Medicine Shandong Provincial Qianfoshan Hospital Shandong University Jinan 250014 Shandong Province China shandong Province Key Lab of Tumor Target Molecule Jinan Central Hospital Affiliated to Shandong University Jinan 250013 Shandong Province China Central Laboratory Jinan Central Hospital Affiliated to Shandong University Jinan 250013 Shandong Province China Department of Pathology Shandong Provincial Qianfoshan Hospital Shandong University Jinan 250014 Shandong Province China Department of Respiratory Provincial Hospital Affiliated to Shandong University Jinan 250021 Shandong Province China Department of Pathology Jinan Central Hospital Affiliated to Shandong University Jinan 250013 Shandong Province China Medical Research and Laboratory Diagnostic Center Jinan Central Hospital Affiliated to Shandong University Jinan 250013 Shandong Province China

AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis. METHODS: The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues. RESULTS: DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated (83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024). CONCLUSION: DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation.

关键词： Differentiated embryo chondrocyte 1 Hepatocellular carcinoma Differentiation Immunohistochemistry

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α5-nAChR/HIF-1α/VEGF axis involved in nicotine-induced lung cancer cell proliferation

α5-nAChR/HIF-1α/VEGF axis involved in nicotine-induced lun...

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第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Xiao-Li Ma Ying Jia shan-shan Zu Yan-Fei Jia yun-shan wang Central lab Jinan Central Hospital Affiliated to Shandong University

Objective:Lung cancer is a common form of lung malignant tumor,the mortality rate is the highest among the cancer ***-wide association studies(GWAS) showed that mutation of nicotinic acetylcholine receptor genomic cluster CHRNA5/A3/B4 locates in chromosomal region 15 q has closed relation with smoking-related lung *** CHRNA5 gene encoding a5-nAChR is particularly relevant to lung *** are five polymers composed of alpha and beta subunits homology or heteromeric,which universally expressed in central and peripheral nervous ***,more function researches of nAChRs are in the normal tissues than in the *** the function of nAChRs subunits in the lung cancer cells could be clear,the therapy on the basis of the nAChRs may provide new research ideas and therapeutic targets for the treatment of lung *** study aimed to explore the influence of small interfering RNA(siRNA) down-regulatingα5-nAChR expression on nicotine-induced HIF-1α and VEGF expression of human lung cancer in vitro and cell proliferation and apoptosis in the lung ***:α5-nAChR、HIF-1α and VEGF mRNA expressions were detected by Real Time-PCR and protein expression were detected by western blot or by ELISA after various concentration nicotine treatment on A549 *** fragment of CHRNA5 encoding a5-nAChR was constructed and transfected into human lung cancer FA549 cell line with lipofectamine *** inhibition effect was detected by Real Time-PCR and Western *** CHRNA5-siRNA transfection,expressions of α5-nAChR,HIF-1α and VEGF were detected by Real Time-PCR and Western blot respectively and the expression of VEGF was detected by ***-induced A549 cell proliferation was detected by MTT ***:(1) Real Time-PCR showed that expressions of a5-nAChR,HIF-1α and VEGF were significantly increased at1μmol/L nicotine treatment compared with other concentration groups(P<0.05).Western blot showed that expression of a5-nAChR and HIF-1α

关键词： Cholinergic receptor nicotinic alpha5(CHRNA5) α5-nAChR HIF-1α VEGF Nicotine Lung cancer

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Magnetic Properties of Heteropoly Complexes α-Na_(m)H_(n)[GaW_(9)M_(3)(H_(2)O)_(3)O_(37)]·16H_(2)O(M=Co(Ⅱ),Mn(Ⅱ),Cr(Ⅲ))

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Chemical Research in Chinese Universities 1997年第4期13卷 78-81页

作者： wang Li ** , LIU Zong rui ** , ZHOU yun shan and wang En bo *** (Department of Chemistry, Northeast Normal University, Changchun, 130024) (State Key Laboratory of Coordination Chemistry, Nanjing, 210093)

ＭａｇｎｅｔｉｃＰｒｏｐｅｒｔｉｅｓｏｆＨｅｔｅｒｏｐｏｌｙＣｏｍｐｌｅｘｅｓα┐ＮａｍＨｎ［ＧａＷ９Ｍ３（Ｈ２Ｏ）３Ｏ３７］·１６Ｈ２Ｏ（Ｍ＝Ｃｏ（Ⅱ），Ｍｎ（Ⅱ），Ｃｒ（Ⅲ））＊ＷＡＮＧＬｉ＊＊，ＬＩＵＺｏｎｇ－... 详细信息

ＭａｇｎｅｔｉｃＰｒｏｐｅｒｔｉｅｓｏｆＨｅｔｅｒｏｐｏｌｙＣｏｍｐｌｅｘｅｓα┐ＮａｍＨｎ［ＧａＷ９Ｍ３（Ｈ２Ｏ）３Ｏ３７］·１６Ｈ２Ｏ（Ｍ＝Ｃｏ（Ⅱ），Ｍｎ（Ⅱ），Ｃｒ（Ⅲ））＊ＷＡＮＧＬｉ＊＊，ＬＩＵＺｏｎｇ－ｒｕｉ＊＊，ＺＨＯＵＹｕｎ－ｓｈａ...

关键词： Tungstogallate heteropoly complex Magnetic susceptibility Antiferromagnetic property

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Study of Sonic hedgehog signaling pathway related molecules in gastric carcinoma

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World Journal of Gastroenterology 2006年第25期12卷 3965-3969页

作者： Xiao-Li Ma Hai-Ji Sun yun-shan wang Shu-Hong Huang Jing-Wu Xie Hong-Wei Zhang Central Laboratory Jinan Central Hospital Jinan 250013 Shandong Province China College of Life Science Shandong Normal University Jinan 250014 Shandong Province China College of Life Science Shandong University Jinan 250010 Shandong Province China

AIM： To study the expression of Sonic hedgehog pathway-related molecules, Sonic hedgehog （Shh） and Glil in gastric carcinoma. METHODS： Expression of Shh in 56 gastric specimens including non-cancerous gastric tissues, gastric adenocarcinoma, gastric squamous cell carcinoma was detected by RT-PCR, in situ hybridization and immunohistochemistry. Expression of Glil was observed by in situ hybridization. RESULTS： The positive rate of Shh and Glil expression was 0.0%, 0.0% in non-cancerous gastric tissues while it was 66.7%, 57.8% respectively in gastric adenocarcinoma, and 100%, 100% respectively in gastric squamous cell carcinoma. There was a significant difference between the non-cancerous gastric tissues and gastric carcinoma （P 〈 0.05）. Elevated expression of Shh and Glil in gastric tubular adenocarcinoma was associated with poorly differentiated tumors while the expression was absent in gastric mucinous adenocarcinoma. CONCLUSION： The elevated expression of Shh and Glil in gastric adenocarcinoma and gastric squamous cell carcinoma shows the involvement of activated Shh signaling in the cellular proliferation of gastric carcinogenesis. It suggests Shh signaling gene may be a new and good target gene for gastric tumor diagnosis and therapy.

关键词： Sonic hedgehog pathway Sonic hedgehog Glil Gastric carcinoma Gastric adenocarcinoma Gastric squamous cell carcinoma

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Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells

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World Journal of Gastroenterology 2005年第38期11卷 6053-6055页

作者： yun-shan wang Xiao-Li Ma Tong-Gang Qi Xiang-Dong Liu Yue-Sheng Meng Guang-Ju Guan Central Laboratory Jinan Central Hospital Clinical Medical College of Shandong University Jinan 250013 Shandong Province China Laboratory of Clinical Molecular Biology The Second Hospital of ShandongUniversity Jinan 250015 Shandong Province China shandong Provincial Hospital Jinan 250020Shandong Province China

AIM: To study the function of α-fetoprotein (AFP) in SMMC-7721 hepatoma ***: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescenceactivated cell sorter (FACS).RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce ***: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.

关键词： siRNAs AFP SMMC-7721 cell Proliferation Apoptosis

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P2Y1 receptor in Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 440-453页

作者： shan Luo Yifei wang Tatsuhiro Hisatsune Department of Integrated Biosciences The University of TokyoKashiwaJapan

Alzheimer’s disease is the most frequent form of dementia characterized by the deposition of amyloid-beta plaques and neurofibrillary tangles consisting of hyperphosphorylated *** amyloid-beta plaques has been a primary direction for developing Alzheimer’s disease treatments in the last ***,existing drugs targeting amyloid-beta plaques have not fully yielded the expected results in the clinic,necessitating the exploration of alternative therapeutic *** evidence unravels that astrocyte morphology and function alter in the brain of Alzheimer’s disease patients,with dysregulated astrocytic purinergic receptors,particularly the P2Y1 receptor,all of which constitute the pathophysiology of Alzheimer’s *** receptors are not only crucial for maintaining normal astrocyte function but are also highly implicated in neuroinflammation in Alzheimer’s *** review delves into recent insights into the association between P2Y1 receptor and Alzheimer’s disease to underscore the potential neuroprotective role of P2Y1 receptor in Alzheimer’s disease by mitigating neuroinflammation,thus offering promising avenues for developing drugs for Alzheimer’s disease and potentially contributing to the development of more effective treatments.

关键词： astrocytes neuroinflammation P2Y1 receptor purinergic receptor

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长期大强度运动对心室miR-155和miR-146a表达的影响

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体育科学 2017年第7期37卷 37-43,59页

作者：饶志坚常芸潘珊珊王世强上海体育学院运动科学学院上海200438 国家体育总局体育科学研究所北京100061 湖南工业大学体育学院湖南株洲412000

目的:研究长期大强度运动对心室miR-155和miR-146a表达的影响,探讨miR-155和miR-146a在调节运动性心肌纤维化和炎症中的作用。方法:48只8周龄雄性SD大鼠,随机分为对照组(Sed)和大强度运动组(IE),每组又分为8周、12周和16周,共6组,每组8... 详细信息

目的:研究长期大强度运动对心室miR-155和miR-146a表达的影响,探讨miR-155和miR-146a在调节运动性心肌纤维化和炎症中的作用。方法:48只8周龄雄性SD大鼠,随机分为对照组(Sed)和大强度运动组(IE),每组又分为8周、12周和16周,共6组,每组8只。对照组自由活动,大强度组以速度28 m/min,坡度10°的条件每天运动1 h,每周运动5天。最后一次运动后24 h后麻醉处死,迅速分离心脏,取左、右心室。采用RT-PCR法检测大鼠左、右心室miR-155和miR-146a及它们相应下游靶基因SOCS1和IRAK1 m RNA的表达水平。结果:长期大强度运动后,大鼠右心室miR-155表达水平显著升高,而左心室miR-155表达水平变化不大;16周大强度运动后,大鼠右心室miR-146a表达水平显著升高,而长期大强度运动对左心室miR-146a表达水平影响不大。长期大强度运动后,左、右心室SOCS1 m RNA的表达水平都没有发生变化;长期大强度运动后,右心室IRAK1 m RNA表达水平显著升高,而左心室IRAK1m RNA的表达水平变化不大。结论:长期大强度运动通过上调右心室miR-155的表达水平促进炎症反应是导致运动性右心室心肌纤维化的可能机制。

关键词：大强度运动心肌纤维化炎症 miR-155 miR-146a

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