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Cloning and Characterization of Genes Coding for Fructan Biosynthesis Enzymes (FBEs) in Triticeae Plants

Agricultural Sciences in China 2010年第3期9卷 313-324页

作者： GAO Xiang SHE Mao-yun yin Gui-xiang YU Yang qiao Wei-hua DU Li-pu YE Xing-guo National Key Facility of Crop Gene Resources and Genetic Improvement Key Laboratory of Crop Genetics and Breeding Ministry of Agriculture/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

Fructan is not only a carbon source for storage but also plays an important role as anti-stress agents in many plant species. Complex fructans having both β-（2,1）- and β-（2,6）-linked fructosyl units accumulate in Triticeae plants commonly. Three enzymes （sucrose： sucrose 1-fructosyltransferase, 1-SST, EC： 2.***.1.99; sucrose： fructan 6-fructosyltransferase, 6- SFT, EC： 2.***.1.10; and fructan： fructan 1-fructosyltransferase, 1-FFT, EC： 2.***.1.100） were involved in fructan biosynthesis in Triticeae plant species. We successfully isolated these genes from tetraploid wheat （Triticum turgidum, genotype： AABB）, common wheat （Triticum aestivum L., genotype： AABBDD） and three wild relatives of common wheat, Triticum urartu Thum. （the origin of the AA genome）, Aegilops speltoides （Tausch） Gren. （the putative source of the SS genome） and Aegilops tauschii Coss. （the source of the DD genome）. Sequence analysis revealed that all the FBEs （fructan biosynthetic enzymes） had three highly conserved functional motifs except 1-SST （EU981912） from tetraploid wheat species only with conserved DPNG. Low pI （isoelectric point） and potential N-glycosylation sites were predicted, which were crucial for protein compartmentation and post-translational process. Analysis on subcelluar localization signals showed that only 6-SFT had vacuolar-directed signal. Sequences alignment result showed that 1-SST and 1-FFT were more conservative and had closer relationship each other, while 6-SFT was more active during the evolution processing. According to the syntenic relationship between wheat and rice genome, FBEs were predicated to be located on the homeologous group 6 and group 2 chromosomes. Expression profile confirmed that expression of all the three FBEs were drought-stress induced. This study can assist to establish a useful theoretical platform for cold- or drought-tolerant improvement of wheat by modulating FBEs expression.

关键词： fructans fructan biosynthesis enzymes gene structure wheat evolution

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Seasonal prediction skills of FIO-ESM for North Pacific sea surface temperature and precipitation

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Acta Oceanologica Sinica 2019年第1期38卷 5-12页

作者： Yiding Zhao Xunqiang yin Yajuan Song Fangli qiao College of Oceanic and Atmospheric Sciences Ocean University of China First Institute of Oceanography Ministry of Natural Resources Laboratory for Regional Oceanography and Numerical Modeling Pilot National Laboratory for Marine Science and Technology (Qingdao) Key Laboratory of Marine Science and Numerical Modeling Ministry of Natural Resources

The seasonal prediction of sea surface temperature(SST) and precipitation in the North Pacific based on the hindcast results of The First Institute of Oceanography Earth System Model(FIO-ESM) is assessed in this *** Ensemble Adjusted Kalman Filter assimilation scheme is used to generate initial conditions, which are shown to be reliable by comparison with the observations. Based on this comparison, we analyze the FIO-ESM 6-month hindcast results starting from each month of 1993–2013. The model exhibits high SST prediction skills over most of the North Pacific for two seasons in advance. Furthermore, it remains skillful at long lead times for midlatitudes. The reliable prediction of SST can transfer fairly well to precipitation prediction via air-sea *** average skill of the North Pacific variability(NPV) index from 1 to 6 months lead is as high as 0.72(0.55) when El Ni?o-Southern Oscillation and NPV are in phase(out of phase) at initial conditions. The prediction skill of the NPV index of FIO-ESM is improved by 11.6%(23.6%) over the Climate Forecast System, Version 2. For seasonal dependence, the skill of FIO-ESM is higher than the skill of persistence prediction in the later period of prediction.

关键词： seasonal prediction North Pacific sea surface temperature precipitation FIO-ESM climate model

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Digital-intensive RFIC design techniques for transmitters in ISSCC 2023

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Journal of Semiconductors 2023年第4期44卷 8-9页

作者： yun yin Hongtao Xu State Key Laboratory of Integrated Chips and Systems Department of MicroelectronicsFudan UniversityShanghai 201203China

Sub-6 GHz multi-standard wireless communication and millimeter-wave(mm-wave)wireless transmission provide users with multi-functionality and unprecedented wireless *** CMOS process scaling down,system-on-chip(SoC)implementation of wireless systems along with RFIC functionality is highly desirable for low cost and small *** digital transceiver architecture aligns with Moore’s law to provide compact die area,better interface to digital backend and higher efficiency due to the faster switching nature of core devices[1−28].

关键词： RFIC ISSCC millimeter

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Effect of Cr/Mn segregation on pearlite–martensite banded structure of high carbon bearing steel

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International Journal of Minerals,Metallurgy and Materials 2021年第4期28卷 665-675页

作者： yun-long Wang yin-li Chen Wei Yu Institute of Engineering Technology University of Science and Technology BeijingBeijing 100083China Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China

The effect of Cr/Mn segregation on the abnormal banded structure of high carbon bearing steel was studied by reheating and hot *** the use of an optical microscope, scanning electron microscope, transmission electron microscope, and electron probe microanalyzer, the segregation characteristics of alloying elements in cast billet and their relationship with hot-rolled plate banded structure were *** formation causes of an abnormal banded structure and the elimination methods were *** indicate the serious positive segregation of C, Cr, and Mn alloy elements in the *** distribution of Cr/Mn elements could not be achieved after 10 h of heat preservation at 1200℃, and the spacing of the element aggregation area increased, but the segregation index of alloy elements *** alloying element segregation characteristics are present in the banded structure of the hot-rolled *** distinct white band is composed of martensitic *** formation of this abnormal pearlite–martensite banded structure is due to the interaction between the undercooled austenite transformation behavior of hot-rolled metal and the segregation of its alloying *** the air cooling after rolling, controlling the segregation index of alloy elements can reduce or eliminate the abnormal banded structure.

关键词： high carbon bearing steel elements segregation homogenization banded structure

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Thickness-dependent wrinkling of PDMS films for programmable mechanochromic responses

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Nano Research 2020年第7期13卷 1882-1888页

作者： Zhiwei Li yun Liu Melinda Marin Yadong yin Department of Chemistry University of CaliforniaRiversideCA 92521USA

We report a remarkable thickness-dependent wrinkling behavior of oxygen plasma-treated polydimethylsiloxane(PDMS)flms,in which an energy barrier separates the wrinkling mechanics into two *** thick films,the film wrinkles with a constant periodicity which can be precisely predicted by the classic nonlinear finite *** the film thickness below 1 mm leads to nonuniform wrinkles with an increasing periodicity which gives rise to random scattering and transparency changes under mechanical *** tuning the flm thickness,we were able to control both the quality and size of the periodic wrinkles and further design mechanochromic devices featuring briliant structural colors and programmable colorimetric *** work sheds light on the fundamental understanding of the wrinkling mechanics of bilayer systems and their intriguing mechanochromic applications.

关键词： wrinkle polydimethylsiloxane(PDMS) structural colors mechanochromic thickness-dependent

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A comparison of two global ocean-ice coupled models with different horizontal resolutions

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Acta Oceanologica Sinica 2013年第8期32卷 1-11页

作者： SHU Qi qiao Fangli SONG Zhenya yin Xunqiang College of Physical and Environmental Oceanography Ocean University of China First Institute of Oceanography State Oceanic Administration Key Laboratory of Marine Science and Numerical Modeling State Oceanic Administration

A global eddy-permitting ocean-ice coupled model with a horizontal resolution of 0.25° by 0.25° is estab- lished on the basis of Modular Ocean Model version 4 （MOM4） and Sea Ice Simulator （SIS）. Simulation results are compared with those of an intermediate resolution ocean-ice coupled model with a horizontal resolution of about 1° by 1°. The results show that the simulated ocean temperature, ocean current and sea ice concentration from the eddy-permitting model are better than those from the intermediate resolu- tion model. However, both the two models have the common problem of ocean general circulation models （OGCMs） that the majority of the simulated summer sea surface temperature （SST） is too warm while the majority of the simulated subsurface summer temperature is too cold. Further numerical experiments show that this problem can be alleviated by incorporating the non-breaking surface wave-induced vertical mixing into the vertical mixing scheme for both eddy-permitting and intermediate resolution models.

关键词： eddy-permitting model intermediate resolution model non-breaking surface wave-induced vertical mixing

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Torsion Instability of Anisotropic Cylindrical Tissues with Growth

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Acta Mechanica Solida Sinica 2019年第5期32卷 621-632页

作者： Sang Ye Si-Fan yin Bo Li Xi-qiao Feng Institute of Biomechanics and Medical Engineering AMLDepartment of Engineering MechanicsTsinghua UniversityBeijing 100084China

Growth shapes soft tissues not only through mass addition or volume expansion but also through deformation instabilities and consequent morphological *** this paper,we probe the torsion instability of an anisotropically growing tube with fiber reinforcement,which mimics many tubular organs in animals or *** derive the Stroh formulation for the incremental boundary value problem and numerically solve it using the surface impedance method.A linear st ability analysis is conducted to investigate the critical condition for the onset of *** thresholds of helical wrinkling are calculated in terms of growth ratio and external *** effect of fibers on the critical state under axial stretching is *** is found that the tangential growth tends to enhance the critical torsion angle but has a weak influence on the critical longitudinal mode of wrinkling,which,however,can be remarkably affected by the axial *** study can help understand the formation of helical morphologies in biological mat erials and provide cues for engineering desired st ruc tu res or devices.

关键词： Biological material Tube Growth Instability Stroh formulation

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Implications of T cell-mediated tumor killing genes for molecular heterogeneity and clinical stratification in lung adenocarcinoma

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Genes & Diseases 2024年第4期11卷 39-41页

作者： Tong qiao Beining yin Jun Liang Li Wei Department of Thoracic Surgery Henan Provincial People's HospitalZhengzhouHenan 450000China Reproductive Medicine Center The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Department of Breast Surgery Henan Provincial People's HospitalZhengzhouHenan 450000China

Due to the profound heterogeneity exhibited amongst patients with lung adenocarcinoma(LUAD),considerable variances in clinical efficacy emerge.1 For the aims of precision medicine,a clinical instrument to delineate distinct disease phenotypes and anticipate susceptibility to intervention is *** cytotoxicity mediated through T lymphocytes constitutes a pivotal mechanism of anti-tumoral immunity and the foundation for cancer immunotherapy.?Numerous genes regulating the sensitivity of tumor cells to killing mediated by T lymphocytes(GSTKKs)are conducive to satisfying the aforesaid medical necessity.

关键词： clinical mediated adenocarcinoma

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Redefined generalized fuzzy ideals of near-rings

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Applied Mathematics(A Journal of Chinese Universities) 2010年第3期25卷 341-348页

作者： ZHAN Jian-ming yin yun-qiang Department of Mathematics Hubei Institute for Nationalities Enshi 445000 China College of Mathematics and Information Sciences East China Institute of Technology Fuzhou Jiangxi 344000 China

With a new idea, we redefine generalized fuzzy subnear-rings （ideals） of a near- ring and investigate some of its related properties. Some new characterizations are given. In particular, we introduce the concepts of strong prime （or semiprime）（∈, ∈∨ q）-fuzzy ideals of near-rings, and discuss the relationship between strong prime （or semiprime）（∈, ∈∨ q）-fuzzy ideals and prime （or semiprime）（∈, ∈∨ q）-fuzzy ideals of near-rings.

关键词： Near-ring subnear-ring (ideal) (∈^- ∈^-∨ q^-)-fuzzy subnear-ring (ideal) prime (semiprime) (∈ ∈∨ q)-fuzzy subnear-ring (ideal).

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Equilibrium of Organic Matter in Heavy Fraction for Three Long-term Experimental Field Soils in China

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Pedosphere 2006年第2期16卷 177-184页

作者： yin yun-Feng CAI Zu-Cong State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China）.

Considerable evidence that the soil organic matter （OM） level in agricultural soils will gradually over time reach an equilibrium state under certain bioclimatic conditions and for certain cropping systems has been accumulating. Although models or long-term experiments have been used, this research used physical fractionation procedure to attain an soil OM equilibrium value. To obtain soil OM equilibrium values in the heavy fraction, typical soils from three long-term field experiments at Fengqiu and yingtan State Key Agro-Ecological Experimental Stations in China were studied using a simple density fractionation procedure and employing the Langmuir equation. Results for the fluvo-aquic soil with organic fertilizer treatments indicated that the soil OM equilibrium value in the heavy fraction was twofold more than that in the inorganic treatments; however, for the paddy soil developed on red soil the OM equilibrium value in the heavy fraction for both treatments was almost identical. It suggested that for fiuvo-aquic soils the increased potential of OM for the heavy fraction in the long run was larger for the organic than the inorganic fertilizer applications, whereas for paddy soils developed on red soils under the same conditions the present OM content in the heavy fraction was at or close to this equilibrium level for all treatments, and increased potential was very limited.

关键词： equilibrium value heavy fraction Langmuir equation soil organic matter

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