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Mining of candidate genes for grape berry cracking using a genome-wide association study

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Journal of Integrative Agriculture 2022年第8期21卷 2291-2304页

作者： ZHANG Chuan WU Jiu-yun CUI Li-wen FANG Jing-gui College of Horticulture Nanjing Agricultural UniversityNanjing 210095P.R.China Turpan Research Institute of Agricultural Sciences Xinjiang Academy of Agricultural SciencesTurpan 838000P.R.China College of Agro-grassland Sciences Nanjing Agricultural UniversityNanjing 210095P.R.China

Fruit cracking is a common phenomenon during the growth and development of horticultural crops that seriously affects fruit yield and quality.However,there are few studies on the mining of candidate genes related to berry cracking.To better understand the genetic basis of grape berry cracking,we conducted a genome-wide association study(GWAS)of grape varieties.Based on the mixed linear model(MLM),we detected five single nucleotide polymorphism(SNP)loci associated with berry-cracking index and two SNP loci associated with berry-cracking type in two years.These loci were mainly distributed on four chromosomes,namely 1,2,3,and 18,and were associated with ten unique candidate berry-cracking genes.The gene expression patterns indicated that the candidate genes in the susceptible berrycracking variety were more abundant than in the resistant berry-cracking variety.Grape berry-cracking is a complex trait controlled by multiple genes,mainly including genes involved in polygalacturonase,copper transporter,and receptor-like proteins.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking,so the present study helps to further elucidate the genetic mechanism of berry cracking.

关键词： berry-cracking index berry-cracking type GWAS MLM SNP

减磷施肥配施生物炭对南方酸性稻作土壤磷素形态的影响

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中国土壤与肥料 2024年第1期 19-27页

作者：苏贵锐荣飞龙张薇张满云吴爱平陈法霖洞庭湖区农村生态系统健康湖南省重点实验室湖南农业大学资源环境学院湖南长沙410128 湖南农业大学农学院湖南长沙410128

针对南方酸性稻作土壤专性吸附和固定磷素能力强的问题,采用微区试验,以正常施磷(75 kg/hm^(2))不施生物炭为对照,探讨不同磷肥水平(P2O575、60、45、30 kg/hm^(2))和生物炭配施(40、60、80 t/hm^(2))对土壤不同形态磷素含量的影响。结... 详细信息

针对南方酸性稻作土壤专性吸附和固定磷素能力强的问题,采用微区试验,以正常施磷(75 kg/hm^(2))不施生物炭为对照,探讨不同磷肥水平(P2O575、60、45、30 kg/hm^(2))和生物炭配施(40、60、80 t/hm^(2))对土壤不同形态磷素含量的影响。结果表明:(1)减磷施肥配施生物炭后全磷、有效磷、树脂磷、碳酸氢钠无机磷及稀盐酸磷的含量显著增加,残留态磷的含量显著降低。(2)磷肥水平≥45 kg/hm^(2)时,配施生物炭处理的全磷和有效磷含量较对照分别显著提高17.1%~46.7%和32.5%~76.3%。(3)磷肥水平≥45 kg/hm^(2)时,配施生物炭处理的树脂磷和碳酸氢钠无机磷的含量较对照分别显著增加50.8%~160.3%和36.1%~118.3%。(4)减磷施肥导致氢氧化钠有机磷含量较对照下降38.4%~39.8%;处理间氢氧化钠无机磷的含量无显著差异。(5)配施生物炭后稀盐酸磷含量较对照显著提高57.9%~352.1%;减磷施肥处理的浓盐酸有机磷含量显著低于正常施磷的处理组合;施磷量为30 kg/hm^(2)时,浓盐酸无机磷的含量显著低于其他处理组合;配施生物炭显著降低残留态磷的含量。(6)土壤全磷、有效磷的含量与树脂磷、碳酸氢钠无机磷、稀盐酸磷的含量呈显著正相关。综上所述,添加生物炭有利于提高南方酸性稻作土壤的磷素含量,促进有机磷向无机磷的转化,提高土壤磷素有效性,在此基础上磷肥施用可低至45 kg/hm^(2)。

关键词：生物炭减磷施肥水稻土磷分级

Genomic Epidemiology of SARS-CoV-2 in Pakistan

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 727-740页

作者： Shuhui Song Cuiping Li Lu Kang Dongmei Tian Nazish Badar Wentai Ma Shilei Zhao Xuan Jiang Chun Wang Yongqiao Sun Wenjie Li Meng Lei Shuangli Li Qiuhui Qi Aamer Ikram Muhammad Salman Massab Umair Huma Shireen Fatima Batool Bing Zhang Hua Chen yun-gui yang Amir Ali Abbasi Mingkun Li Yongbiao Xue Yiming Bao China National Center for Bioinformation Beijing 100101China National Genomics Data Center Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Department of Virology and Immunology National Institute of HealthIslamabad 45500Pakistan National Center for Bioinformatics Programme of Comparative and Evolutionary GenomicsFaculty of Biological SciencesQuaid-i-Azam UniversityIslamabad 45320Pakistan Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovation Academy of Seed DesignChinese Academy of SciencesBeijing 100101China

COVID-19 has swept globally and Pakistan is no exception.To investigate the initial introductions and transmissions of the SARS-CoV-2 in Pakistan,we performed the largest genomic epidemiology study of COVID-19 in Pakistan and generated 150 complete SARS-CoV-2 genome sequences from samples collected from March 16 to June 1,2020.We identified a total of 347 mutated positions,31 of which were over-represented in Pakistan.Meanwhile,we found over 1000 intra-host single-nucleotide variants(iSNVs).Several of them occurred concurrently,indicating possible interactions among them or coevolution.Some of the high-frequency iSNVs in Pakistan were not observed in the global population,suggesting strong purifying selections.The genomic epidemiology revealed five distinctive spreading clusters.The largest cluster consisted of 74 viruses which were derived from different geographic locations of Pakistan and formed a deep hierarchical structure,indicating an extensive and persistent nation-wide transmission of the virus that was probably attributed to a signature mutation(G8371T in ORF1ab)of this cluster.Furthermore,28 putative international introductions were identified,several of which are consistent with the epidemiological investigations.In all,this study has inferred the possible pathways of introductions and transmissions of SARS-CoV-2 in Pakistan,which could aid ongoing and future viral surveillance and COVID-19 control.

关键词： SARS-CoV-2 Virus Molecular evolution Haplotype network Pakistan

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沉锂母液回收锂工艺研究进展

稀有金属与硬质合金 2024年第2期52卷 11-17,44页

作者：李世春曲直刘大锐高桂梅张云峰洪雨张洁中国神华能源股份有限公司哈尔乌素露天煤矿国家能源集团煤炭伴生资源综合利用研究中心内蒙古鄂尔多斯010399 北京航天动力研究所北京100076 准格尔旗职业高级中学内蒙古鄂尔多斯010399

在采用碳酸盐沉淀法生产碳酸锂过程中,约有20%的锂会损失在沉锂母液中。从沉锂母液中回收锂,既可以解决碳酸锂生产工艺废水的排放问题,又可以提升锂的总回收率,对国家战略能源储备和企业生产具有十分重要的意义。各种沉锂母液回收锂工艺... 详细信息

在采用碳酸盐沉淀法生产碳酸锂过程中,约有20%的锂会损失在沉锂母液中。从沉锂母液中回收锂,既可以解决碳酸锂生产工艺废水的排放问题,又可以提升锂的总回收率,对国家战略能源储备和企业生产具有十分重要的意义。各种沉锂母液回收锂工艺中,析钠法工艺简单易操作,但基本只能生产工业二级碳酸锂产品,产品价值不高;沉淀法大大提升了母液中锂的回收率,但磷酸盐和氟化盐的使用会引入大量磷酸根离子和氟离子,对环境具有很大的不利影响;吸附法工艺简单、锂选择性好、回收率高,但存在吸附剂实际吸附容量小和溶损严重的问题;萃取法具有锂选择性强、提取率高的优点,但传统萃取体系均采用无机酸反萃,反萃酸度高,导致设备腐蚀严重,作业环境差。开发低成本、高效率、绿色环保的回收技术,是未来沉锂母液回收锂的必然发展方向。

关键词：沉锂母液锂回收碳酸锂析钠法沉淀法吸附法萃取法

军队特勤人员高同型半胱氨酸血症检出率及影响因素分析

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中国疗养医学 2024年第6期33卷 45-48页

作者：赵涛秦桂琼杨俊伟张芸陈玉洁中国人民解放军联勤保障部队桂林康复疗养中心广西桂林541002

目的探讨特勤人员高同型半胱氨酸血症(HHcy)检出率及影响因素,为开展针对性的健康干预提供科学依据。方法以2023年9月至2024年1月在某疗养中心参加特勤体检鉴定的618例特勤疗养员为研究对象,内容包括人口学特征、生活方式、体格检查... 详细信息

目的探讨特勤人员高同型半胱氨酸血症(HHcy)检出率及影响因素,为开展针对性的健康干预提供科学依据。方法以2023年9月至2024年1月在某疗养中心参加特勤体检鉴定的618例特勤疗养员为研究对象,内容包括人口学特征、生活方式、体格检查和实验室检测等资料。结果调查人群同型半胱氨酸(Hcy)平均水平12.10(10.00~14.60)μmol/L,HHcy检出率为23.79%,平均年龄(34.21±7.46)岁,均为男性。多因素Logistic回归分析结果显示,吸烟(OR=1.833,95%CI 1.152,2.915)、勤务类别(OR=1.697,95%CI 1.240,2.323)、低密度脂蛋白胆固醇(LDL-C)(OR=5.764,95%CI 3.229,10.292)、尿酸(UA)(OR=1.693,95%CI 1.116,2.570)、胆红素(BIL)(OR=2.015,95%CI 1.330,3.052)、肌酐(CR)(OR=3.164,95%CI 1.624,6.162)是HHcy的影响因素。结论吸烟、勤务类别、LDL-C、UA、BIL和CR是HHcy的影响因素,应对存在高危因素的人群采取相应措施,尽早进行健康干预,以降低心脑血管等慢性疾病发生的风险。

关键词：特勤人员高同型半胱氨酸血症影响因素健康干预

不同提取方法对大果木姜子挥发油成分及其抑菌活性的影响

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粮食与油脂 2024年第7期37卷 30-35,55页

作者：钱贵芸郭江涛张永萍刘杰徐剑韩伟贵州中医药大学药学院贵州贵阳550025 国家苗药工程技术研究中心贵州贵阳550025 贵州中药炮制与制剂工程技术研究中心贵州贵阳550025

比较超声提取(UE)、微波提取(ME)、索氏提取(XZ)、水蒸气蒸馏(SD)提取的大果木姜子挥发油的化学成分,并考察其对大肠杆菌(Eco)、金黄色葡萄球菌(Sta)、枯草芽孢杆菌(Bs)、铜绿假单胞菌(Pak)的抑菌能力。结果表明:SD、UE、ME、XZ提取的... 详细信息

比较超声提取(UE)、微波提取(ME)、索氏提取(XZ)、水蒸气蒸馏(SD)提取的大果木姜子挥发油的化学成分,并考察其对大肠杆菌(Eco)、金黄色葡萄球菌(Sta)、枯草芽孢杆菌(Bs)、铜绿假单胞菌(Pak)的抑菌能力。结果表明:SD、UE、ME、XZ提取的大果木姜子挥发油分别鉴定出69、65、72、60种成分,相对含量分别占其总挥发油的91.382%、88.323%、85.709%、89.864%。4种挥发油对Eco的最小抑菌浓度(MIC)为SD

关键词：大果木姜子挥发油超声提取微波提取索氏提取水蒸气蒸馏抑菌活性

碳化硅陶瓷基复合材料表面环境障涂层结合强度

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材料工程 2024年第2期52卷 198-206页

作者：苏超群邓龙辉刘若愚蒋佳宁云海涛李归苗小锋陈文博易出山刘俐董淑娟曹学强中国航发南方工业有限公司湖南株洲412002 武汉理工大学硅酸盐建筑材料国家重点实验室武汉430070

SiC陶瓷基复合材料(SiC-based ceramic matrix composites,SiC-CMC)是发展高推重比航空发动机理想的高温结构材料。为了防止发动机服役环境下燃气(富含H2O和O_(2))对SiC-CMC的腐蚀,需要在其表面制备抗水氧腐蚀、抗燃气冲刷和抗热冲击性... 详细信息

SiC陶瓷基复合材料(SiC-based ceramic matrix composites,SiC-CMC)是发展高推重比航空发动机理想的高温结构材料。为了防止发动机服役环境下燃气(富含H2O和O_(2))对SiC-CMC的腐蚀,需要在其表面制备抗水氧腐蚀、抗燃气冲刷和抗热冲击性能优异的环境障涂层(environmental barrier coatings,EBCs)。在评价EBCs性能的诸多因素中,其与SiC-CMC基体之间的结合强度是一个重要技术指标,但结合强度的极限值一直未被探究清楚。本工作研究影响结合强度的主要因素,包含SiC-CMC基体状态、单晶Si的拉伸强度极限,以及Si黏结层的制备工艺等,获得了制备最高结合强度的有效途径。在EBCs与SiC-CMC组成的体系中,基体内部SiC纤维布之间的界面是结合强度最薄弱的部位,其次是EBCs的Si层。整个体系的结合强度极限值是15 MPa,它是单晶Si在[400]晶向的拉伸强度极限。采用大气等离子喷涂或者超音速火焰喷涂的Si黏结层结合强度相似,均低于同样工艺制备的莫来石或Yb2Si2O7涂层。

关键词：碳化硅复合材料环境障涂层结合强度

Large-scale real-world radio signal recognition with deep learning

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Chinese Journal of Aeronautics 2022年第9期35卷 35-48页

作者： Ya TU yun LIN Haoran ZHA Ju ZHANG Yu WANG Guan gui Shiwen MAO College of Information and Communication Engineering Harbin Engineering UniversityHarbin 150001China College of Electronic Science and Engineering National University of Defense TechnologyChangsha 410073China College of Telecommunications and Information Engineering Nanjing University of Posts and TelecommunicationsNanjing 210003China Department of Electrical and Computer Engineering Auburn UniversityAuburn 36849USA

In the past ten years, many high-quality datasets have been released to support the rapid development of deep learning in the fields of computer vision, voice, and natural language processing. Nowadays, deep learning has become a key research component of the Sixth-Generation wireless systems(6G) with numerous regulatory and defense applications. In order to facilitate the application of deep learning in radio signal recognition, in this work, a large-scale real-world radio signal dataset is created based on a special aeronautical monitoring system-Automatic Dependent Surveillance-Broadcast(ADS-B). This paper makes two main contributions. First, an automatic data collection and labeling system is designed to capture over-the-air ADS-B signals in the open and real-world scenario without human participation. Through data cleaning and sorting, a high-quality dataset of ADS-B signals is created for radio signal recognition. Second, we conduct an in-depth study on the performance of deep learning models using the new dataset, as well as comparison with a recognition benchmark using machine learning and deep learning methods.Finally, we conclude this paper with a discussion of open problems in this area.

关键词： Signal recognition Radio signal dataset Automatic Dependent Surveillance-Broadcast(ADS-B) Deep learning Recognition benchmark

Multimodal therapy strategies based on hydrogels for the repair of spinal cord injury

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Military Medical Research 2022年第5期9卷 603-617页

作者： Yan Wang Hong-Qian Lv Xuan Chao Wen-Xin Xu yun Liu gui-Xia Ling Peng Zhang Shenyang Pharmaceutical University 103 Wenhua RoadShenyang 110016China Center for Nanotechnology in Drug Delivery Eshelman School of PharmacyUniversity of North Carolina at Chapel HillChapel HillNC 27599USA

Spinal cord injury(SCI)is a serious traumatic disease of the central nervous system,which can give rise to the loss of motor and sensory function.Due to its complex pathological mechanism,the treatment of this disease still faces a huge challenge.Hydrogels with good biocompatibility and biodegradability can well imitate the extracellular matrix in the microenvironment of spinal cord.Hydrogels have been regarded as promising SCI repair material in recent years and continuous studies have confirmed that hydrogel-based therapy can effectively eliminate inflammation and promote spinal cord repair and regeneration to improve SCI.In this review,hydrogel-based multimodal therapeutic strategies to repair SCI are provided,and a combination of hydrogel scaffolds and other therapeutic modalities are discussed,with particular emphasis on the repair mechanism of SCI.

关键词： Spinal cord injury Injectable hydrogels Hydrogel scaffolds Multimodal therapy