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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

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The Crop Journal 2024年第2期12卷 569-582页

作者： Suting wu Htin Kyaw Zhijun Tong Yirong Yang Zhiwei Wang Liying Zhang Lihua Deng Zhiguo Zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

Effects of confining pressure on acoustic emission and failure characteristics of sandstone

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International Journal of Mining Science and Technology 2021年第5期31卷 963-974页

作者： Zhen Huang Qixiong Gu Yufan wu yun wu Shijie Li Kui Zhao Rui Zhang School of Resources and Environment Engineering Jiangxi University of Science and TechnologyGanzhou 341000China School of Earth Sciences and Engineering Nanjing UniversityNanjing 210023China School of Earth Sciences and Engineering Sun Yat-Sen UniversityGuangzhou 510275China Kunming Survey Design and Research Institute Co.Ltd.of CREECKunming 650200China

In this study,uniaxial and triaxial compression acoustic emission(AE)tests were implemented to investigate the AE effect and failure characteristics of sandstone under different confining pressures(σ3).The evolution of AE parameters in the rock failure process and fracture fractal dimension characteristics after failure were *** results revealed that the activity of the AE signal is strongly related toσ*** evolution of the Ib value can be divided into the I-fluctuation,II-stability,and III-decrease *** the first stage,the Ib value of the AE was relatively high,and the AE energy was ***,the Ib value tended to be stable;however,the fluctuation amplitude decreased,and the AE energy rapidly *** the stage of decrease,the AE energy sharply increased before the load approached the peak value,and the Ib value significantly decreased and dropped to the lowest point before the peak ***σ3 increased,the rock’s failure mode changed from tensile failure to shear failure and became more *** the confining pressure increased,the shape dimension decreased,and the order degree of rock failure *** confining pressure exerted a certain control effect on the rock failure.

关键词： Sandstone Acoustic emission Ib value Failure mode Fractal dimension

长链非编码RNAMALAT1调控星形胶质细胞增殖和凋亡及对MAPK/ERK1,2信号通路的影响

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军事医学 2024年第5期48卷 347-354页

作者：胡辉王雪吴雨涵董华丰张玲韦爱君谢方赵云孙兆炜钱令嘉军事科学院军事医学研究院北京100850

目的探讨肺腺癌转移相关转录本1(MALAT1)的表达对星形胶质细胞(C8-D1A)增殖和凋亡,以及丝裂原活化蛋白激酶(MAPK)/细胞外信号调节激酶(ERK1,2)通路的影响。方法通过慢病毒载体和质粒载体在C8-D1A细胞中分别敲低及过表达MALAT1基因,用实... 详细信息

目的探讨肺腺癌转移相关转录本1(MALAT1)的表达对星形胶质细胞(C8-D1A)增殖和凋亡,以及丝裂原活化蛋白激酶(MAPK)/细胞外信号调节激酶(ERK1,2)通路的影响。方法通过慢病毒载体和质粒载体在C8-D1A细胞中分别敲低及过表达MALAT1基因,用实时荧光定量PCR(qPCR)法检测敲低及过表达效率、细胞增殖相关标志物Ki67、微染色体维持蛋白2(MCM2)、细胞核增殖抗原(PCNA)、凋亡相关蛋白含半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)、B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)的表达变化,CCK-8、流式细胞术检测敲低及过表达MALAT1后对C8-D1A细胞增殖和凋亡的影响。免疫荧光染色法检测Caspase-3和Ki67蛋白表达,Western印迹法检测细胞凋亡相关蛋白Caspase-3、Bax、Bcl-2及ERK1/2、p-ERK1/2、p38MAPK、p-p38MAPK蛋白表达变化情况。结果与对照组相比,过表达MALAT1抑制细胞增殖并诱导细胞凋亡,其G0/G1期、G2/M期比例升高,S期比例降低,Caspase-3蛋白表达升高,p-ERK1/2蛋白表达降低,Ki67、PCNA mRNA表达量降低;而敲低MALAT1后细胞增殖显著增加并抑制细胞凋亡,其S期比例增高、G2/M期比例降低,Caspase-3、Bax蛋白表达降低,p-ERK1/2、p-p38MAPK蛋白表达升高,Ki67、MCM2、PCNA mRNA表达量增加(P<0.05)。结论MALAT1基因可能通过调控MAPK/ERK1,2信号通路进而促进星形胶质细胞凋亡并抑制其增殖。

关键词：肺腺癌转移相关转录本1 星形胶质细胞丝裂原活化蛋白激酶细胞外信号调节激酶细胞凋亡细胞增殖

Camrelizumab,apatinib and hepatic artery infusion chemotherapy combined with microwave ablation for advanced hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3481-3495页

作者： Meng-Xuan Zuo Chao An Yu-Zhe Cao Jia-Yu Pan Lu-Ping Xie Xin-Jing Yang Wang Li Pei-Hong wu Department of Minimally Invasive Interventional Therapy Sun Yat-sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina Department of Interventional Ultrasound Chinese General PLA HospitalBeijing 100853China Department of Medical Imaging and Interventional Radiology Sun Yatsen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina

BACKGROUND Hepatic arterial infusion chemotherapy and camrelizumab plus apatinib(TRIPLET protocol)is promising for advanced hepatocellular carcinoma(Ad-HCC).However,the usefulness of microwave ablation(MWA)after TRIPLET is still *** To compare the efficacy and safety of TRIPLET alone(T-A)vs TRIPLET-MWA(TM)for *** From January 2018 to March 2022,217 Ad-HCC patients were retrospectively *** them,122 were included in the T-A group,and 95 were included in the T-M group.A propensity score matching(PSM)was applied to balance *** survival(OS)was compared using the Kaplan-Meier curve with the log-rank *** overall objective response rate(ORR)and major complications were also *** After PSM,82 patients were included both the T-A group and the T-M *** ORR(85.4%)in the T-M group was significantly higher than that(65.9%)in the T-A group(P<0.001).The cumulative 1-,2-,and 3-year OS rates were 98.7%,93.4%,and 82.0%in the T-M group and 85.1%,63.1%,and 55.0%in the T-A group(hazard ratio=0.22;95%confidence interval:0.10-0.49;P<0.001).The incidence of major complications was 4.9%(6/122)in the T-A group and 5.3%(5/95)in the T-M group,which were not significantly different(P=1.000).CONCLUSION T-M can provide better survival outcomes and comparable safety for Ad-HCC than T-A.

关键词： Hepatic arterial infusion chemotherapy Hepatocellular carcinoma Molecular targeting agent Programmed cell death protein 1 inhibitors Microwave ablation

Gut microbiota affects the estrus return of sows by regulating the metabolism of sex steroid hormones

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Journal of Animal Science and Biotechnology 2024年第2期15卷 578-599页

作者： Min Liu Jia Zhang yunyan Zhou Shuqi Xiong Mengqing Zhou Lin wu Qin Liu Zhe Chen Hui Jiang Jiawen Yang Yuxin Liu Yaxiang Wang Congying Chen Lusheng Huang National Key Laboratory of Swine Genetic Improvement and Germplasm Innovation Jiangxi Agricultural UniversityNanchang 330045China

Background Sex hormones play important roles in the estrus return of post-weaning *** studies have demonstrated a complex and bi-directional regulation between sex hormones and gut ***,the extent to which the gut microbiota affects estrus return of post-weaning sows is largely *** In this study,we first screened 207 fecal samples from well-phenotyped sows by 16S rRNA gene sequencing and identified significant associations between microbes and estrus return of post-weaning *** metagenomic sequencing data from 85 fecal samples,we identified 37 bacterial species that were significantly associated with estrus *** returning sows were characterized by increased abundances of *** and *** and decreased abundances of ***,***,and *** changes in gut microbial composition significantly altered the functional capacity of steroid hormone biosynthesis in the gut *** results were confirmed in a validation *** changes in sex steroid hormones and related compounds were found between normal and non-return sows via metabolome *** integrated analysis of differential bacterial species,metagenome,and fecal metabolome provided evidence that normal return-associated bacterial species *** and Prevotella *** in the degradation of pregnenolone,progesterone,and testosterone,thereby promoting estrogen ***,the microbial metabolites related to sow energy and nutrient supply or metabolic disorders also showed relationships with sow estrus *** An integrated analysis of differentially abundant bacterial species,metagenome,and fecal metabolome revealed the involvement of *** and Prevotella *** sow estrus *** findings provide deep insight into the role of gut microbiota in the estrus return of post-weaning sows and the complex cross-talk between gut microbiota and sex hormones,suggesting that the manipulatio

关键词： Estrus return Fecal metabolome Gut microbiota Metagenomics Sex steroid hormones Sow

Mechanical property and thermal shock behavior of tungsten-yttria-stabilized zirconia cermet fabricated by spark plasma sintering

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Tungsten 2024年第1期6卷 134-140页

作者： Lin-Chao Zhang Ke Jing Rui Liu Zhuo-Ming Xie Jun-Feng Yang Gang Li Xian-Ping Wang Xue-Bang wu Qian-Feng Fang Chang-Song Liu Key Laboratory of Materials Physics Institute of Solid State PhysicsHFIPSChinese Academy of SciencesHefei 230031China University of Science and Technology of China Hefei 230026China Science and Technology on Reactor Fuel and Materials Laboratory Nuclear Power Institute of ChinaChengdu 610041China

The cermet fuels have been considered as a potential key component for the nuclear thermal propulsion,and the homogeneity of the fuel particles in the metal matrix plays a crucial role in stabilizing the structure at extremely high *** this work,liquid paraffin was used as additive to improve the distribution of yttria-stabilized zirconia(YSZ,an appropriate surrogate for UO_(2) fuel)microspheres in the tungsten(W)matrix,and the W-YSZ cermet wafers(volume ratio 1:1)with a relative density of 97.6%were fabricated by spark plasma sintering with a specifically designed *** effects of the paraffin dosage(0-5 wt.%)on the homogeneity,microstructure,mechanical properties,and the thermal conductivity of W-YSZ cermet were *** W-YSZ sample with 2 wt.%paraffin shows the highest homogeneity and exhibits the best comprehensive properties,including the ultimate tensile strength of 132.2 MPa at 600℃,the bending strength of455 MPa and thermal conductivity of 50 W·m^(-1)·K^(-1)at room ***,the cermet could keep structurally sound after thermal shocked at a heat load of 20 MW·m^(-2).These results would be helpful for the design and optimization of the cermet fuels in the nuclear thermal propulsion.

关键词： Nuclear thermal propulsion Tungsten Cermet Liquid paraffin Thermal shock

Clinical evolution of antisynthetase syndrome-associated interstitial lung disease after COVID-19 in a man with Klinefelter syndrome:A case report

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World Journal of Clinical Cases 2024年第6期12卷 1144-1149页

作者： Xiang-Xiang wu Jian Cui Shi-Yao Wang Tian-Tian Zhao Ya-Fei Yuan Long Yang Wei Zuo Wen-Jian Liao Department of Respiratory and Critical Care The First Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina China-Japan Friendship Jiangxi Hospital Nanchang 330006Jiangxi ProvinceChina Department of Pulmonary and Critical Care Medicine Center of Respiratory MedicineChina-Japan Friendship HospitalBeijing 100029China

BACKGROUND This study presents a case of rapidly developing respiratory failure due to antisynthetase syndrome(AS)following coronavirus disease 2019(COVID-19)in a 33-year-old man diagnosed with Klinefelter syndrome(KS).CASE SUMMARY A 33-year-old man with a diagnosis of KS was admitted to the Department of Pulmonary and Critical Care Medicine of a tertiary hospital in China for fever and shortness of breath 2 wk after the onset of *** tomography of both lungs revealed diffuse multiple patchy heightened shadows in both lungs,accompanied by signs of partial bronchial *** next-generation sequencing of the bronchoalveolar lavage fluid suggested absence of pathogen.A biopsy specimen revealed organizing pneumonia with alveolar septal ***,extensive auto-antibody tests showed strong positivity for anti-SSA,anti-SSB,anti-Jo-1,and *** multidisciplinary discussions,the patient received a final diagnosis of AS,leading to rapidly progressing respiratory *** This study underscores the clinical progression of AS-associated interstitial lung disease subsequent to viral infections such as COVID-19 in patients diagnosed with KS.

关键词： Antisynthetase syndrome COVID-19 Klinefelter syndrome Interstitial lung disease Anti-Jo-1 Case report

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煤体中瓦斯水合固化的力学作用研究进展

煤炭学报 2024年第2期49卷 720-738页

作者：吴强张保勇黑龙江科技大学安全工程学院黑龙江哈尔滨150022

为更好地完善我国煤与瓦斯突出预测与防治方法,基于煤与瓦斯突出综合作用假说,提出了瓦斯水合固化防治煤与瓦斯突出的方法。该方法核心是将煤层中瓦斯固化生成瓦斯水合物,不仅能降低瓦斯压力,而且能够提高煤体强度,以达到减弱或消除煤... 详细信息

为更好地完善我国煤与瓦斯突出预测与防治方法,基于煤与瓦斯突出综合作用假说,提出了瓦斯水合固化防治煤与瓦斯突出的方法。该方法核心是将煤层中瓦斯固化生成瓦斯水合物,不仅能降低瓦斯压力,而且能够提高煤体强度,以达到减弱或消除煤与瓦斯突出危险性的目的。实现了煤体中瓦斯水合物的生成以及含瓦斯水合物煤体力学性质-渗透率原位测试,提出了煤体中瓦斯水合物生成及力学性质-渗透率测试技术及含瓦斯水合物煤体三轴压缩数值建模技术。瓦斯水合固化热力学和动力学条件是防突的理论基础,煤层瓦斯水合物稳定储存是技术前提,瓦斯压力降低及煤体力学性质改善是防突关键,重点围绕水合固化煤体交叉力学问题进行总结。分析认为:(1)瓦斯水合固化防突技术理论框架已经初步形成,并利用数值模拟手段初步探究了瓦斯水合固化对煤体力学特征改善的细观机理;(2)现阶段已证实煤体中水合物生成不仅能降低瓦斯压力,而且能改善其力学性质,高饱和度对提高煤体峰值强度明显;(3)瓦斯水合物生成经历快速、缓慢和稳定3个阶段,且水合物的生成会造成煤体中瓦斯渗流通道阻塞,导致其渗透率降低;(4)高瓦斯压力、高CH4体积分数不仅有助于提高水合物饱和度而且会延缓水合物分解,有助于水合物的稳定存在。需要注意的是,瓦斯水合固化技术防突的可靠度仍需大量重复试验验证,以建立普适化的数据库。综合现有研究结果,对水合固化防突研究目前仍存在的局限性与挑战进行了讨论,并且给出了进一步的研究方向。

关键词：煤与瓦斯突出瓦斯水合固化含瓦斯水合物煤体力学性质离散元

海底火山喷发大气冲击波激发的轴对称海啸波数值模型

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力学学报 2024年第7期56卷 2015-2028页

作者：吴蓓蓓刘桦上海交通大学工程力学系水动力学教育部重点实验室上海200240

海底火山喷发引发海啸具有多源激发机制,包括水下爆炸、边坡失稳、火山口坍塌、碎屑流、地震以及大气扰动.为了深入认识轴对称移动气压扰动激发海啸的机理,探究气压扰动参数对水波形态的影响规律,本文在柱面孤立波Boussinesq模型的基础... 详细信息

海底火山喷发引发海啸具有多源激发机制,包括水下爆炸、边坡失稳、火山口坍塌、碎屑流、地震以及大气扰动.为了深入认识轴对称移动气压扰动激发海啸的机理,探究气压扰动参数对水波形态的影响规律,本文在柱面孤立波Boussinesq模型的基础上,考虑水面移动气压扰动作用,建立了由火山喷发激发的轴对称移动气压场所驱动的海啸波数值模型.利用不同地形上柱面波的传播算例验证了本模型的精确性与稳定性.数值模拟了2022年汤加火山喷发大气扰动激发海啸事件,并与太平洋DART浮标实测数据比较,较好地复演了大气扰动驱动海啸波的远场传播过程,并讨论了波动在深水区的色散行为.研究了轴对称气压扰动的径向移速、强度及尺度对波动演化特征的影响,结果显示:气压移速与浅水波速的相对大小显著影响波形,当二者接近时将激发Proudman共振.在共振条件下,波幅与径向传播距离呈近似线性增长关系,波幅放大因子随气压尺度增大而减小.在远离共振条件时,气压强度和尺度对波幅放大因子的影响相对较小,受波能流守恒约束柱面自由波幅则沿程衰减,受迫波幅值近似按气压衰减规律变化.

关键词：移动气压扰动柱面波 Boussinesq方程 Proudman共振汤加火山海啸