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Structural Analysis and Identification of Cis-Elements of Rice osRACD Gene

Acta Biochimica et Biophysica Sinica 2004年第3期36卷 191-198页

作者： yun-zhe xu, rui-lin you and nai-hu wu institute of genetics and developmental biology, chinese academy of sciences, beijing 100080, china 2College of Life sciences, Peking University, beijing 100871, china institute of genetics and developmental biology Chinese Academy of Sciences Beijing 100080 China College of Life sciences Peking University Beijing 100871 China

The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice (PGMR), Nongken 58S, encoded a rice (Oryza sativa L. ssp. japonica) small GTPase belonging to the Rac/Rho family. Inverse PCR was performed to amplify a fragment about 1.4 kb in 5' upstream region of the osRACD promoter. Deletion mutation and gel mobility shift assay characterized two fragments (–799 to –686 nt, and –686 to –431 nt) in the osRACD promoter that could be involved in its transcriptional regulation. When these two deletion fragments were used as probe respectively, a retarded band appeared in the nuclear extracts of fertile 58S rice under short day (58S-SD). Whereas no retarded band was shown in the nuclear extracts of sterile 58S rice under long day (58S-LD). Competition assay indicated that the factors in the retarded bands binding to these two fragments were the same trans-acting factor (termed rice factor, RF). The binding affinity of RF was affected by phosphorylation and was higher in SD-growth rice than that of LD-growth rice.

关键词： photoperiod-sensitive male genic sterile rice osRACD gene photoperiod fertility transformation cis-element

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