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颈动脉狭窄和认知功能障碍的关系研究进展

山东第一医科大学（山东省医学科学院）学报 2024年第7期45卷 438-445页

作者：庄源张雯张国良王永祥王默山东第一医科大学附属省立医院血管外科山东济南250021 山东第一医科大学附属省立医院神经内科山东济南250021

近年来颈动脉狭窄的发病率逐步增高,认知功能和颈动脉狭窄之间的关系一直受到人们的关注。较多研究表明,症状性颈动脉狭窄(symptomatic carotid stenosis,SCS)可引起认知功能障碍。然而,无症状性颈动脉狭窄(asymptomatic carotid stenos... 详细信息

近年来颈动脉狭窄的发病率逐步增高,认知功能和颈动脉狭窄之间的关系一直受到人们的关注。较多研究表明,症状性颈动脉狭窄(symptomatic carotid stenosis,SCS)可引起认知功能障碍。然而,无症状性颈动脉狭窄(asymptomatic carotid stenosis,ACS)对认知功能的影响及影响程度目前尚未有明确的结论,可能的发病机制包括脑低灌注、脑微栓塞和脑白质高信号等,颈动脉血运重建能否改善颈动脉狭窄导致的认知功能障碍也尚未得出确切的结论。在颈动脉狭窄患者出现明显的症状前尽早发现并积极干预,能够避免认知功能下降并改善患者的预后。本文从颈动脉狭窄和认知功能之间的关系、发病机制和颈动脉血运重建等方面检索了国内外相关文献,重点从ACS对认知功能的影响和颈动脉血运重建对认知功能的改善方面进行综述。

关键词：颈动脉狭窄颈动脉血运重建认知功能障碍

中医药对HIV/AIDS合并慢性非传染性疾病的实践与探讨

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时珍国医国药 2024年第5期35卷 1211-1214页

作者：刘梅君宋夕元郭润泽雷颀郝明玲金书情孟鹏飞马秀霞岳静宇胡之纲徐立然河南中医药大学河南郑州450000 河南中医药大学第一附属医院河南郑州450003 河南省中医药防治病毒性疾病重点实验室河南郑州450008 河南省中医中药治疗艾滋病项目办公室河南郑州450003

目的ART疗法的普及应用使得HIV/AIDS患者的生存率显著提升、预期寿命延长。随着HIV/AIDS患者出现老龄化,该病合并慢性非感染性疾病的患病率逐年上升,艾滋病防治工作依然严峻。既往研究表明中医药在防治艾滋病和慢性疾病方面有显著优势,... 详细信息

目的ART疗法的普及应用使得HIV/AIDS患者的生存率显著提升、预期寿命延长。随着HIV/AIDS患者出现老龄化,该病合并慢性非感染性疾病的患病率逐年上升,艾滋病防治工作依然严峻。既往研究表明中医药在防治艾滋病和慢性疾病方面有显著优势,文章通过分析HIV/AIDS合并心血管疾病、慢性阻塞性肺疾病、高脂血症、糖尿病、恶性肿瘤等常见的慢性非传染性疾病的研究现状,探讨中医药防治HIV/AIDS合并常见的慢性非传染性疾病目前存在的问题,并提出了相应的对策,以期为深入开展中医药防治HIV/AIDS合并慢性非感染性疾病的研究提供依据及参考。

关键词：人类免疫缺陷病毒艾滋病(获得性免疫缺陷综合征) 慢性非传染性疾病中医药心血管疾病慢性阻塞性肺疾病高脂血症糖尿病肾脏疾病恶性肿瘤

Striatal CDK5 Regulates Cholinergic Neuron Activation and Dyskinesia-like Behaviors through BK Channels

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Research 2023年第4期2023卷 739-753页

作者： Chu Tong Peng-Xiang Min Qian Zhang Ru-Xin Gu Yao-Hai Wen Yi Shi Yu-Huan Bao Xiang Chen Yi-Xuan Zhang Xing-Feng Mao hao-Yang Yuan Xiu-Xiu liu Takuya Sasaki li Zhang Feng Han Ying-Mei Lu Department of Physiology School of Basic Medical SciencesNanjing Medical UniversityNanjing211166China International Joint Laboratory for Drug Target of Critical Illnesses School of PharmacyNanjing Medical UniversityNanjing211166China Department of Geriatrics Nanjing Brain Hospital Affiliated to Nanjing Medical UniversityNanjing210029China Institute of Pharmacology and Toxicology College of Pharmaceutical SciencesZhejiang UniversityHangzhou310058China Gusu School Nanjing Medical UniversitySuzhou Municipal HospitalThe Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou215002China Department of Pharmacology Graduate School of Pharmaceutical SciencesTohoku UniversitySendai980-8578Japan Institute of Brain Science the Affiliated Brain Hospital of Nanjing Medical UniversityNanjing211166China.

Disturbance of the cholinergic system plays a crucial role in the pathological progression of neurological diseases that cause dyskinesia-like ***,the molecular mechanisms underlying this disturbance remain ***,we showed that cyclin-dependent kinase 5(Cdk5)was reduced in cholinergic neurons of midbrain according to the single-nucleus RNA sequencing *** levels of CDK5 also decreased in patients with Parkinson’s disease accom{4.ied by motor ***,Cdk5 deficiency in cholinergic neurons triggered paw tremors,abnormal motor coordination,and motor balance deficits in *** symptoms occurred along with cholinergic neuron hyperexcitability and increases in the current density of large-conductance Ca2.-activated K+channels(BK channels).Pharmacological inhibition of BK channels restrained the excessive intrinsic excitability of striatal cholinergic neurons in Cdk5-deficient ***,CDK5 interacted with BK channels and negatively regulated BK channel activity via phosphorylation of *** of CDK5 expression in striatal cholinergic neurons reduced dyskinesia-like behaviors in ChAT-Cre;Cdk5f/f ***,these findings indicate that CDK5-induced phosphorylation of BK channels involves in cholinergic-neuronmediated motor function,providing a potential new therapeutic target for treating dyskinesia-like behaviors arising from neurological diseases.

关键词： Cdk5 Neuron CDK5

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人工智能助力检验医学

中华检验医学杂志 2021年第10期44卷 882-886页

作者：郝晓柯李卓空军军医大学西京医院全军临床检验医学研究所西安710032 西安医学院第一附属医院检验科西安710077

人工智能技术在检验医学领域的应用现已显示出了巨大潜力,但也面临着诸多挑战和困境。了解人工智能的基本原理、评价方法、应用场景及其优势和局限性,有助于更好地指导工作实践。在不久的将来,AI将会全方位应用于临床检验中,使检验医学... 详细信息

人工智能技术在检验医学领域的应用现已显示出了巨大潜力,但也面临着诸多挑战和困境。了解人工智能的基本原理、评价方法、应用场景及其优势和局限性,有助于更好地指导工作实践。在不久的将来,AI将会全方位应用于临床检验中,使检验医学在疾病诊断、疗效监测、预后判断等方面发挥更加精准的作用。在AI技术的助力之下,检验医学的发展未来可期。

关键词：人工智能检验医学机器学习

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溴己新治疗新型冠状病毒肺炎的潜在机制分析

中国药学杂志 2020年第9期55卷 679-684页

作者：杨勇沈浩赵山美子王东王浩然四川省医学科学院四川省人民医院药学部个体化药物治疗四川省重点实验室成都610072 电子科技大学医学院成都611731 广州医科大学附属广州市妇女儿童医疗中心儿科研究所广州510623 沈阳药科大学中药学院沈阳110016 纽兰生物科技有限公司山东威海264200

目的2.19年12.新型冠状病毒肺炎(COVID-19)暴发,疫情在世界范围内迅速蔓延,寻找有效抗病毒药物成为治疗新型冠状病毒的迫切需求。方法笔者借助已有病毒学文献,对严重急性呼吸系统综合征冠状病毒2.SARS-CoV-2.感染通路进行了系统的总结... 详细信息

目的2.19年12.新型冠状病毒肺炎(COVID-19)暴发,疫情在世界范围内迅速蔓延,寻找有效抗病毒药物成为治疗新型冠状病毒的迫切需求。方法笔者借助已有病毒学文献,对严重急性呼吸系统综合征冠状病毒2.SARS-CoV-2.感染通路进行了系统的总结,并且运用SWISSDOCK分子模拟方法对关键靶点和上市药物进行系统的虚拟药物筛选。结果研究发现,TMPRSS2.ACE2.路是SARS-CoV-2.毒进入肺部和其他组织细胞最高效和主要的途径。而呼吸科祛痰药物溴己新体外可强烈抑制TMPRSS2.白酶(EC50:0.75μmol·L^-1)。溴己新不良反应少且还具备促进肺部内源活性物质释放,维持肺泡功能,促进排痰等有益作用,适合联合用药。结论溴己新具有独特的潜在抗病毒机制,应当深入进行临床研究,发挥其对COVID-19预防,治疗和预后的作用。

关键词：溴己新氯喹新型冠状病毒肺炎严重急性呼吸系统综合征冠状病毒2 抗病毒机制

5-Aza-2.-dc预处理致敏百草枯对V79细胞活性氧及Bcl-2.Bax表达的影响

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昆明医科大学学报 2016年第10期37卷 10-13页

作者：王晔曲艳陈美琼郝丽昆明医科大学附属延安医院急诊医学科云南昆明650051

目的研究DNA甲基化酶抑制剂5-氮杂-2.-脱氧胞苷(5-Aza-2.-dc)与百草枯联合对V79细胞作用后的活性氧含量及抗凋亡Bcl-2.白和促凋亡Bax蛋白表达的影响.方法 V79细胞经传代培养后分为5-Aza-2.-dc作用组(A组)、百草枯作用组(B组)、5-Aza-2.... 详细信息

目的研究DNA甲基化酶抑制剂5-氮杂-2.-脱氧胞苷(5-Aza-2.-dc)与百草枯联合对V79细胞作用后的活性氧含量及抗凋亡Bcl-2.白和促凋亡Bax蛋白表达的影响.方法 V79细胞经传代培养后分为5-Aza-2.-dc作用组(A组)、百草枯作用组(B组)、5-Aza-2.-dc加百草枯作用组(C组,即先应用5-Aza-2.-dc对V79细胞进行预处理12.,然后给予百草枯作用12.)、对照组(D组).采用2.7二氯荧光黄双乙酸盐(DCFH-DA)荧光探针装载,流式细胞仪测定各实验组V79细胞内活性氧含量,Western blot检测各实验组V79细胞内抗凋亡Bcl-2.白和促凋亡Bax蛋白的表达情况.结果 5-Aza-2.-dc与百草枯联合作用组(C组)V79细胞内活性氧含量、抗凋亡Bcl-2.白、促凋亡Bax蛋白表达与5-Aza-2.-dc组(A组)、百草枯组(B组)和对照组(D组)比较有统计学差异(P2.白表达、抗凋亡Bcl-2.白/促凋亡Bax蛋白比值降低,活性氧含量和促凋亡Bax蛋白表达升高.结论 5-Aza-2.-dc调控DNA甲基化可能通过活性氧代谢失衡、细胞调亡来促进百草枯对V79细胞的毒性损伤.

关键词： 5-氮杂-2.-脱氧胞苷百草枯 V79细胞活性氧 Bcl-2.Bax

Duct-to-duct biliary reconstruction after radical resection of Bismuth Ⅲ a hilar cholangiocarcinoma

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World Journal of Gastroenterology 2013年第15期19卷 2441-2444页

作者： Wen-Guang Wu Jun Gu Ping Dong jian-Hua Lu Mao-Lan li Xiang-Song Wu jia-Hua Yang lin Zhang Qi-Chen Ding {4. Weng Qian Ding Ying-Bin liu Depatment of General Surgery Xinhua HospitalAffiliated to School of MedicineShanghai Jiaotong University

At present, radical resection remains the only effective treatment for patients with hilar cholangiocarcinoma. The surgical approach for R0 resection of hilar cholangiocarcinoma is complex and diverse, but for the biliary reconstruction after resection, almost all surgeons use Roux-en-Y hepaticojejunostomy. A viable alternative to Roux-en-Y reconstruction after radical resection of hilar cholangiocarcinoma has not yet been proposed. We report a case of performing duct-to-duct biliary reconstruction after radical resection of Bismuth Ⅲa hilar cholangiocarcinoma. End-to-end anastomosis between the left hepatic duct and the distal common bile duct was used for the biliary reconstruction, and a singlelayer continuous suture was performed along the bile duct using 5-0 prolene. The patient was discharged favorably without biliary fistula 2.wk later. Evidence for tumor recurrence was not found after an 18 mo follow- up. Performing bile duct end-to-end anastomosis in hilar cholangiocarcinoma can simplify the complex digestive tract reconstruction process.

关键词： Hilar cholangiocarcinoma Biliary reconstruction Duct-to-duct Radical resection Digestive tract reconstruction Hepaticojejunostomy Bile duct anastomosis

Establish and authenticate an animal model for the triple negative breast cancer with high expression of PD-L1 and EGFR

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Journal of Hainan Medical university 2019年第2期25卷 6-10页

作者： Shi-jian wang {2. {2. Tie-Feng Xu first affiliated {2. and the Oncological Institute of Hainan Medical university Haikou570102HainanChina Department of General Surgery the Second Affiliated Hospital of Hainan Medical UniversityHaikou570311HainanChina Attatched Qianfoshan hospital of Shandong university Jinan250014ShandongChina

Objective:To establish and authenticate an animal model for the triple negative breast cancer patients with high expression of PD-L1 and ***: the humanized breast cancer cell MDA-MB-2.1 was trypsinized and the supernatant was removed, washed twice with Hank's, and finally 1×105 cells were adjusted with physiological saline and injected into the nude mice's fat pads. The tumor's formation time, growth, infiltration and metastasis of mice were observed and recorded in detail. At the end of the 8th-week the tumor-bearing mice were killed to collect fresh cancer tissues and divide them into two after the anesthesia. The cancer tissue was soaked and fixed by 10% formalin solution and embedded by paraffin to make about 4.m paraffin sections, utilized HE staining and immunohistochemistry to detect the expression of ER, PR, Her-2. Ki-67, EGFR and ***: Orthotopic injection of MDA-MB-2.1 cells could induce tumor formation on the 6th days. And the measuring was carried out on the 9th days;the tumor size was about (0.970 ± 0.073) cm3 at week 4. (3.2.0±0.14.) cm3 at week 8;and there was more uniform in growth , rich in blood vessels, less necrosis and no ulcers of the tumor. HE staining indicated invasive ductal carcinoma;immunohistochemistry showed ER (-), PR (-), Her-2.(-), Ki-67 (+, 85%), EGFR (+ 90%) and PD-L1 (+ 80%).Conclusions:We successfully established and authenticated a humanized TNBC animal model with high expression of PD-L1 and EGFR , and perhaps offered an animal model to the patients' precision medicine.

关键词： TNBC PD-L1 EGFR Precision medicine Animal model

HGD—A型痔疮电子治疗仪治疗痔疮60例报告

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暨南大学学报（自然科学与医学版） 1991年第2期12卷 69-72页

作者：宋超文陈贞巧暨南大学附属医院外科

应用HGD—A型痔疮电子治疗仪治疗痔疮60例,其中内痔34.,混合痔2.例,总有效率为100％,显效痊愈率达96.67％。电子治疗仪的治疗原理是以微电流的电解效应为主与电热效应的综合作用,使痔组织血管内血栓形成、纤维化、电凝坏死、萎缩,从而... 详细信息

应用HGD—A型痔疮电子治疗仪治疗痔疮60例,其中内痔34.,混合痔2.例,总有效率为100％,显效痊愈率达96.67％。电子治疗仪的治疗原理是以微电流的电解效应为主与电热效应的综合作用,使痔组织血管内血栓形成、纤维化、电凝坏死、萎缩,从而达到治疗目的。

关键词：痔疮电子治疗仪