检索结果-南通市图书馆

H-NS family protein MvaU downregulates phenazine-1-carboxylic acid(PCA)biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas strain PA1201

引用

Synthetic and Systems Biotechnology 2021年第4期6卷 262-271页

作者： yun-ling fang Ying Cui Lian Zhou Chitti Thawai Tatheer Alam Naqvi Hong-Yan Zhang Ya-Wen He State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSJTU-Shanghai Nong Le Joint R&D Center on Biopesticides and BiofertilizersShanghai Jiao Tong University(SJTU)Shanghai200240China Department of Biology Faculty of ScienceKing Mongkut’s Institute of Technology LadkrabangBangkokThailand Department of Biotechnology COMSATS University IslamabadAbbottabad CampusAbbottabadPakistan Shanghai Nong Le Biological Products Company Limited Shanghai201419China

Histone-like nucleoid-structuring(H-NS)proteins are key regulators in gene expression silencing and in nucleoid compaction.The H-NS family member proteins MvaU in Pseudomonas aeruginosa are thought to bind the same AT-rich regions of chromosomes and function to coordinate the control of a common set of genes.Here,we explored the molecular mechanism by which MvaU controls PCA biosynthesis in P.aeruginosa PA1201.We present evidence suggesting that MvaU is self-regulated.Deletion of mvaU significantly increased PCA production,and PCA production sharply decreased when mvaU was over-expressed.MvaU transcriptionally repressed phz2 cluster expression and consequently reduced PCA biosynthesis.β-galactosidase assays confirmed that base pairing near the􀀀35 box is required when MvaU regulates PCA production in PA1201.Electrophoretic mobility shift assays(EMSA)and additional point mutation analysis demonstrated that MvaU directly bound to an AT-rich motif within the promoter of the phz2 cluster.Chromatin immunoprecipitation(ChIP)analysis also indicated that MvaU directly bound to the P5 region of the phz2 cluster promoter.MvaU repression of PCA biosynthesis was independent of QscR and OxyR in PA1201 and neither PCA or H2O2 were the environmental signals that induced mvaU expression.These findings detail a new MvaU-dependent regulatory pathway of PCA biosynthesis in PA1201 and provide a foundation to increase PCA fermentation titer by genetic engineering.

关键词： Pseudomonas Phenazine-1-carboxylic acid phz cluster MvaU Regulatory network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Quantifying peripapillary vessel density and retinal nerve fibre layer in type 1 diabetic children without clinically detectable retinopathy using OCTA

引用

International Journal of Ophthalmology(English edition) 2024年第2期17卷 278-281页

作者： ling Chen yun Feng Sha-Sha Zhang Yan-fang Liu Ping Lin Xi’an Children’s Hospital Affiliated to Xi’an Jiaotong University Xi’an 710002Shaanxi ProvinceChina

AIM:To quantify changes in radial peripapillary capillary vessel density(ppVD)and the peripapillary retinal nerve fiber layer(pRNFL)in children with type 1 diabetes without clinical diabetic retinopathy by optical coherence tomography angiography(OCTA),providing a basis for early retinopathy in children with type 1 diabetes.METHODS:This was a retrospective study.A total of 30 patients(3–14y)with type 1 diabetes without clinical diabetic retinopathy(NDR group)were included.A total of 30 age-matched healthy subjects were included as the normal control group(CON group).The HbA1c level in the last 3mo was measured once in the NDR group.The pRNFL thickness and ppVD were automatically measured,and the mean pRNFL and ppVD were calculated in the nasal,inferior,temporal,and superior quadrants.The changes in ppVD and pRNFL in the two groups were analyzed.RESULTS:Compared with CON group,the nasal and superior ppVDs decreased in the NDR group(all P0.05).Person and Spearman correlation analysis of ppVD and pRNFL thickness in each quadrant of the NDR group showed a positive correlation between nasal and superior(all P0.05).There was no significant correlation between the HbA1c level and ppVD and pRNFL in any quadrant(all P>0.05).There was no significant correlation between the course of diabetes mellitus and ppVD and pRNFL in any quadrant(all P>0.05).CONCLUSION:ppVD and pRNFL decrease in eyes of children with type 1 diabetes before clinically detectable retinopathy and OCTA is helpful for early monitoring.

关键词： diabetic retinopathy children peripapillary vessel density peripapillary retinal nerve fiber layer optical coherence tomography angiography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Improving iconic memory through contrast detection training with HOA-corrected vision

引用

Fundamental Research 2024年第1期4卷 95-102页

作者： ling Gong Junlei Zhao yun Dai Zili Wang fang Hou Yudong Zhang Zhong-Lin Lu Jiawei Zhou State Key Laboratory of Ophthalmology Optometry and Vision ScienceEye HospitalSchool of Ophthalmology and OptometryWenzhou Medical UniversityWenzhou 325027China Institute of Optics and Electronics Chinese Academy of SciencesChengdu 610209China The Key Laboratory of Adaptive Optics Chinese Academy of SciencesChengdu 610209China Chengdu University of Traditional Chinese Medicine Chengdu 610075China Division of Arts and Sciences New York University ShanghaiShanghai 200126China Center for Neural Science Department of PsychologyNew York UniversityNew York 10003United States NYU-ECNU Institute of Brain and Cognitive Science at NYU Shanghai Shanghai 200062China

Iconic memory and short-term memory are not only crucial for perception and cognition,but also of great importance to mental health.Here,we first showed that both types of memory could be improved by improving limiting processes in visual processing through perceptual learning.Normal adults were trained in a contrast detection task for ten days,with their higher-order aberrations(HOA)corrected in real-time.We found that the training improved not only their contrast sensitivity function(CSF),but also their iconic memory and baseline information maintenance for short-term memory,and the relationship between memory and CSF improvements could be well-predicted by an observer model.These results suggest that training the limiting component of a cognitive task with visual perceptual learning could improve visual cognition.They may also provide an empirical foundation for new therapies to treat people with poor sensory memory.

关键词： Perceptual learning Iconic memory Higher-order aberrations Contrast sensitivity Short-term memory

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Construction of CDKN2A-related competitive endogenous RNA network and identification of GAS5 as a prognostic indicator for hepatocellular carcinoma

引用

World Journal of Gastrointestinal Oncology 2024年第4期16卷 1514-1531页

作者： Yong Pan Yi-Ru Zhang ling-yun Wang Li-Na Wu Ying-Qiu Ma Zhou fang Shi-Bo Li Department of Infectious Disease Zhoushan HospitalWenzhou Medical UniversityZhoushan 316021Zhejiang ProvinceChina State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of Zhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Department of Infectious Disease The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035Zhejiang ProvinceChina

BACKGROUND Competitive endogenous RNA(ceRNA)is an innovative way of gene expression modulation,which plays a crucial part in neoplasia.However,the intricacy and behavioral characteristics of the ceRNA network in hepatocellular carcinoma(HCC)remain dismal.AIM To establish a cyclin dependent kinase inhibitor 2A(CDKN2A)-related ceRNA network and recognize potential prognostic indicators for HCC.METHODS The mutation landscape of CDKN2A in HCC was first explored using the cBioPortal database.Differential expression analysis was implemented between CDKN2Ahigh and CDKN2Alow expression HCC samples.The targeted microRNAs were predicted by lncBasev3.0,and the targeted mRNAs were predicted by miRDB,and Targetscan database.The univariate and multivariate analysis were utilized to identify independent prognostic indicators.RESULTS CDKN2A was frequently mutated and deleted in HCC.The single-cell RNA-sequencing analysis revealed that CDKN2A participated in cell cycle pathways.The CDKN2A-related ceRNA network-growth arrest specific 5(GAS5)/miR-25-3p/SRY-box transcription factor 11(SOX11)was successfully established.GAS5 was recognized as an independent prognostic biomarker,whose overexpression was correlated with a poor prognosis in HCC patients.The association between GAS5 expression and methylation,immune infilt-ration was explored.Besides,traditional Chinese medicine effective components targeting GAS5 were obtained.CONCLUSION This CDKN2A-related ceRNA network provides innovative insights into the molecular mechanism of HCC formation and progression.Moreover,GAS5 might be a significant prognostic biomarker and therapeutic target in HCC.

关键词： Hepatocellular carcinoma Competitive endogenous RNA Molecular mechanism Prognosis Biomarker

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prediction of suitable water content in granulation of sintering mixture based on Litster's model

引用

Journal of Iron and Steel Research(International) 2024年第3期31卷 552-560页

作者： fang-lei Dai Xiao-hui Fan Xiao-xian Huang Xu-ling Chen Min Gan Zhi-yun Ji Zeng-qing Sun School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083HunanChina

Suitable water content plays a decisive role in the granulation of sintering mixtures.Herein,a method was proposed to predict the suitable water content for effective granulation on the basis of Litster's granulation model.The granulation effectiveness of a sintering mixture was predicted by the model,with the allowance error of±10%.The effects of the water absorption properties,particle size composition and content of adhesive particles on the suitable water content were studied.The results showed that the allowable error of prediction was within±0.5%compared to the experimentally determined suitable water content.With an increase in adhesive powder content of mixtures with higher water absorption,the suitable water content increased to achieve similar granulation effectiveness.Moreover,as the amount of concentrates increased,the suitable water content first increased and then remained steady.The influence of the water absorption characteristics of the adhesive particles on the suitable water content was less than that of their particle size composition in the mixture.

关键词： Adhesive particle Sintering mixture Granulation effectiveness Suitable water content

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Metal nanoparticles for cancer therapy:Precision targeting of DNA damage

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 1132-1149页

作者： Qian Chen Chunyan fang Fan Xia Qiyue Wang fangyuan Li Daishun ling Institute of Pharmaceutics Hangzhou Institute of Innovative MedicineCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Frontiers Science Center for Transformative Molecules School of Chemistry and Chemical EngineeringNational Center for Translational MedicineShanghai Jiao Tong UniversityShanghai 200240China World Laureates Association(WLA)Laboratories Shanghai 201203China Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province Hangzhou 310009China

Cancer,a complex and heterogeneous disease,arises from genomic instability.Currently,DNA damage-based cancer treatments,including radiotherapy and chemotherapy,are employed in clinical practice.However,the efficacy and safety of these therapies are constrained by various factors,limiting their ability to meet current clinical demands.Metal nanoparticles present promising avenues for enhancing each critical aspect of DNA damage-based cancer therapy.Their customizable physicochemical properties enable the development of targeted and personalized treatment platforms.In this review,we delve into the design principles and optimization strategies of metal nanoparticles.We shed light on the limitations of DNA damage-based therapy while highlighting the diverse strategies made possible by metal nanoparticles.These encompass targeted drug delivery,inhibition of DNA repair mechanisms,induction of cell death,and the cascading immune response.Moreover,we explore the pivotal role of physicochemical factors such as nanoparticle size,stimuli-responsiveness,and surface modification in shaping metal nanoparticle platforms.Finally,we present insights into the challenges and future directions of metal nanoparticles in advancing DNA damage-based cancer therapy,paving the way for novel treatment paradigms.

关键词： DNA damage Metal nanoparticles Nucleus-targeting DNA repair inhibition Immune response Size optimization Stimuli-responsiveness Surface modification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Targeting oxidative stress with natural products: A novel strategy for esophageal cancer therapy

引用

World Journal of Gastrointestinal Oncology 2024年第2期16卷 287-299页

作者： fang Cao Han-ling Zhang Cui Guo Xue-Liang Xu Qiang Yuan Department of Rehabilitation III Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072Sichuan ProvinceChina Department of Rehabilitation Chongqing Fuling District Maternal and Child Health HospitalChongqing 408000China Department of Rehabilitation Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072Sichuan ProvinceChina

Esophageal cancer(ESC)is a malignant tumor that originates from the mucosal epithelium of the esophagus and is part of the digestive tract.Although the exact pathogenesis of ESC has not been fully elucidated,excessive oxidative stress is an important characteristic that leads to the development of many cancers.Abnormal expression of several proteins and transcription factors contributes to oxidative stress in ESCs,which alters the growth and proliferation of ESCs and promotes their metastasis.Natural compounds,including alkaloids,terpenes,polyphenols,and xanthine compounds,can inhibit reactive oxygen species production in ESCs.These compounds reduce oxidative stress levels and subsequently inhibit the oc-currence and progression of ESC through the regulation of targets and pathways such as the cytokine interleukins 6 and 10,superoxide dismutase,the NF-+ACY-kappa+ADs-B/MAPK pathway,and the mammalian Nrf2/ARE target pathway.Thus,targeting tumor oxidative stress has become a key focus in anti-ESC therapy.This review discusses the potential of Natural products(NPs)for treating ESCs and summarizes the application prospects of oxidative stress as a new target for ESC treatment.The findings of this review provide a reference for drug development targeting ESCs.Nonetheless,further high-quality studies will be necessary to determine the clinical efficacy of these various NPs.

关键词： Oxidative stress Natural products Esophageal cancer Reactive oxygen species

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Flexible, efficient, and accurate tests for epidemics

引用

Science China Mathematics 2024年第8期67卷 1881-1898页

作者： Linjiajie fang Bing-Yi Jing Shen ling Qiyue Wang Qing Yang Data Science and Analytics Thrust The Hong Kong University of Science and Technology Department of Statistics and Data Science Southern University of Science and Technology Department of Mathematics and Statistics Dalhousie University Department of Mathematics The Hong Kong University of Science and Technology

Group testing involves discovering a small subset of distinguished subjects from a large population while efficiently reducing the total number of tests. It has been widely used for industrial testing, information technology, and biology, especially epidemic screening. Tests, in reality, are noisy for the presence of false outcomes. Some tests are accurate but time-consuming, while others are cheaper but less accurate. Exactly which test to use is constrained by various considerations, such as availability, cost, accuracy, and efficiency.In this paper, we propose flexible, efficient, and accurate tests(FEATs). FEATs are based on group testing with simple but careful designs by incorporating ideas such as close contact cliques and repeated tests. FEATs could dramatically improve the efficiency or accuracy of existing tests. For example, for accurate but slow tests, the FEAT can improve efficiency multiple times without compromising accuracy. On the other hand,for fast but inaccurate tests, the FEAT can sharply reduce the false-negative rate(FNR) and significantly increase efficiency. Theoretical justifications are provided. We point out some scenarios where the FEAT can be effectively employed.

关键词： epidemiology control blood testing testing efficiency testing accuracy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Survival Analysis of Patients Undergoing Intraoperative Contrast-enhanced Ultrasound in the Surgical Treatment of Malignant Glioma

引用

Current Medical Science 2024年第2期44卷 399-405页

作者： Xu CHEN Ya-ni PENG fang-ling CHENG Dan CAO An-yu TAO Jian CHEN Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Urology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Medical Ultrasound Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective:Complete resection of malignant gliomas is often challenging.Our previous study indicated that intraoperative contrast-enhanced ultrasound(ICEUS)could aid in the detection of residual tumor remnants and the total removal of brain lesions.This study aimed to investigate the survival rates of patients undergoing resection with or without the use of ICEUS and to assess the impact of ICEUS on the prognosis of patients with malignant glioma.Methods:A total of 64 patients diagnosed with malignant glioma(WHO grade HI and IV)who underwent surgery between 2012 and 2018 were included.Among them,29 patients received ICEUS.The effects of ICEUS on overall survival(OS)and progression-free survival(PFS)of patients were evaluated.A quantitative analysis was performed to compare ICEUS parameters between gliomas and the surrounding tissues.Results:The ICEUS group showed better survival rates both in OS and PFS than the control group.The univariate analysis revealed that age,pathology and ICEUS were significant prognostic factors for PFS,with only age being a significant prognostic factor for OS.In multivariate analysis,age and ICEUS were significant prognostic factors for both OS and PFS.The quantitative analysis showed that the intensity and transit time of microbubbles reaching the tumors were significantly different from those of microbubbles reaching the surrounding tissue.Conclusion:ICEUS facilitates the identification of residual tumors.Age and ICEUS are prognostic factors for malignant glioma surgery,and use of ICEUS offers a better prognosis for patients with malignant glioma.

关键词： intraoperative contrast-enhanced ultrasound malignant glioma extent of resection survival prognosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association of preschool children behavior and emotional problems with the parenting behavior of both parents

引用

World Journal of Clinical Cases 2024年第6期12卷 1084-1093页

作者： Su-Mei Wang Shuang-Qin Yan fang-fang Xie Zhi-ling Cai Guo-Peng Gao Ting-Ting Weng fang-Biao Tao Department of Child Health Ma’anshan Maternal and Child Health CenterMa’anshan 243011Anhui ProvinceChina Department of Maternal Child and Adolescent HealthSchool of Public HealthAnhui Medical UniversityHefei 230032Anhui ProvinceChina

BACKGROUND Parental behaviors are key in shaping children’s psychological and behavioral development,crucial for early identification and prevention of mental health issues,reducing psychological trauma in childhood.AIM To investigate the relationship between parenting behaviors and behavioral and emotional issues in preschool children.METHODS From October 2017 to May 2018,7 kindergartens in Ma’anshan City were selected to conduct a parent self-filled questionnaire-Health Development Survey of Preschool Children.Children’s Strength and Difficulties Questionnaire(Parent Version)was applied to measures the children’s behavioral and emotional performance.Parenting behavior was evaluated using the Parental Behavior Inventory.Binomial logistic regression model was used to analyze the association between the detection rate of preschool children’s behavior and emotional problems and their parenting behaviors.RESULTS High level of parental support/participation was negatively correlated with conduct problems,abnormal hyperactivity,abnormal total difficulty scores and abnormal prosocial behavior problems.High level of maternal support/participation was negatively correlated with abnormal emotional symptoms and abnormal peer interaction in children.High level of parental hostility/coercion was positively correlated with abnormal emotional symptoms,abnormal conduct problems,abnormal hyperactivity,abnormal peer interaction,and abnormal total difficulty scores in children(all P0.05),after calculating ratio of odds ratio values.CONCLUSION Our study found that parenting behaviors are associated with behavioral and emotional issues in preschool children.Overall,the more supportive or involved the parents are,the fewer behavioral and emotional problems the children experience;conversely,the more hostile or controlling the parents are,the more behavioral and em

关键词： Children Preschool age Parenting Behavioral Parenting problems

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：