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Relation of hepatic EROD activity and cytochrome P4501A level in Sebastiscus marmoratus exposed to benzo[a]pyrene

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Journal of Environmental Sciences 2008年第1期20卷 101-104页

作者： WANG yun ZHENG Ronghui zuo Zhenghong CHEN Yixin WANG Chonggang Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 China.

This study was designed to investigate the in vivo effects of benzo[a]pyrene （BaP） on hepatic ethoxyresorufin-O-deethylase （EROD） activity and its correlation with cytochrome P4501A （CYP1A） protein levels in Sebastiscus marmoratus, which were exposed through a water column to BaP （10, 100, 1000 ng/L, respectively） or were treated with intraperitoneal injections of BaP （0.5, 1, 5, 10 mg/kg, respectively） every 7 d. The results showed that after 25 d of waterborne exposure to 1000 ng/L BaP, fish hepatic CYP1A levels and EROD activity were significantly induced. In contrast, EROD activity was not altered 7 d after second intraperitoneal injections, whereas, CYP1A protein levels were increased. Dose-dependent increase of biliary BaP metabolites demonstrated that the catalytic activity of CYP1A was induced by treatment with BaP. The lowest observable effect concentration with regard to biliary BaP metabolites （100 ng/L） was much lower than that with reference to EROD activity （1000 ng/L）. The results suggest that biliary polycyclic aromatic hydrocarbon （PAH） metabolites were shown to better reflect the contamination gradients of PAHs than EROD activity. It appeared to be necessary to measure CYP1A protein levels to complement the EROD activity in relevant toxicological assessments.

关键词： BaP BaP metabolites EROD activity CYP1A protein Sebastiscus marmoratus

MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer

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World Journal of Gastroenterology 2015年第15期21卷 4564-4573页

作者： Wei-Dong Lu yun zuo Zhen Xu Min Zhang Department of Oncology Zhangjiagang First People’s Hospital Suzhou University

AIM: To investigate the mechanism by which mi R-19 a is up-regulated in gastric cancer(GC), which plays an oncogenic ***: In the present study, we investigated the role of mi R-19 a in gastric tissues as well as two GC cell lines. In vivo, we detected the basal expression level of mi R-19 a using real-time reverse transcription-PCR(RTPCR), and the relevance between expression of mi R-19 a and clinicopathological information was *** vitro, mi R-19 a was ectopically expressed using overexpression and knock-down ***: Overexpression of mi R-19 a was significantly associated with metastasis of GC and inferior overall prognosis. However, no significant correlation was f o u n d b e t w e e n m i R- 1 9 a e x p r e s s i o n a n d o t h e r characteristics such as age, gender, tobacco, alcohol or tumor size. Cell proliferation, migration and invasion assays showed that overexpression of mi R-19 a promoted the proliferation, migration and invasion, and that overexpression of mi R-19 a promoted the epithelialmesenchymal transition through activating the PI3K/AKT pathway. Blocking the PI3K/AKT pathway could cancel the effect of mi R-19 ***: All together, our results suggest that mi R-19 a could be used as a promising therapeutic target in the treatment of GC.

关键词： Gastric cancer miR-19a PI3K-AKT Epithelialmesenchymaltransition

Tuning the intensity statistics of random speckle patterns

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Chinese Physics B 2019年第5期28卷 327-331页

作者： Fan Meng Yue Zhao yun-zuo Zhang Lei Huo School of Information Science and Technology Shijiazhuang Tiedao University Department of Gastroenterology and Hepatology the Fourth Hospital of Hebei Medical University

Speckle patterns are a fundamental tool in a variety of physical and optical applications. Here, we investigate a method of precisely tuning the intensity statistics of random speckle patterns into a desirable pattern that possesses the same spatial correlation length and similar statistics distribution. This tuning mechanism relies on the derivation of the transform function and transmission matrix, which achieves different contrasts while maintaining the same average value or energy level. The statistics properties of the generated speckle patterns are further investigated by analyzing the standard deviation under different fitting parameters. Precisely tuning the intensity statistics of random speckle patterns could be useful for both fundamental research and practical applications, such as microscopy, imaging, and optical manipulation.

关键词： random speckle pattern intensity statistics local intensity transformation optical manipulation

Hyperbolic Dependence of Moment of Inertia onω2 in Bands Involving vi13/2 Orbital and its Application in Configuration Identification

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Chinese Physics Letters 2000年第4期17卷 261-263页

作者： LU Jing-Bin LIU yun-zuo ZHANG Fan Department of Physics Jilin UniversityChangchun 130023 Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000

The so-called hyperbolic dependence of apparent moment of inertia on square of rotational frequency is developed to the bands involving^13/2 quasineutron around A-60 lighter rare-earth *** can be explained as a characteristic of strongly Coriolis mixing in the high-j unique-parity *** applications of such an effectj the previously proposed assignments of band x of^(160)Ho,π1/2^(-)[541]■vi_(13/2)band of^(170)Ta,as well as band B of 168Lu have been reviewed respectively.

关键词： bands Hyperbolic Configuration

An OpenFlow-based performance-oriented multipath forwarding scheme in datacenters

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Frontiers of Information Technology & Electronic Engineering 2016年第7期17卷 647-660页

作者： Bo LIU Ming CHEN Bo XU Hui HU Chao HU Qing-yun zuo Chang-you XING College of Command Information Systems PLA University of Science and TechnologyNanjing 210007China PLA Academy of National Defense Information Wuhan 430020China

Although dense interconnection datacenter networks(DCNs)(e.g.,Fat Tree) provide multiple paths and high bisection bandwidth for each server pair,the widely used single-path Transmission Control Protocol(TCP)and equal-cost multipath(ECMP) transport protocols cannot achieve high resource utilization due to poor resource excavation and *** this paper,we present LESSOR,a performance-oriented multipath forwarding scheme to improve DCNs' resource *** adopting an Open Flow-based centralized control mechanism,LESSOR computes near-optimal transmission path and bandwidth provision for each flow according to the global network view while maintaining nearly real-time network view with the performance-oriented flow observing *** and comprehensive simulations show that LESSOR can efficiently improve the network throughput,which is higher than ECMP by 4.9%–38.3% under different *** also provides 2%–27.7% improvement of throughput compared with ***,LESSOR decreases the average flow completion time significantly.

关键词： Datacenter network Traffic engineering Open Flow Multipath transmission

Two new species of Polyalthiopsis(Annonaceae) based on morphological characters and phylogenetic evidence,with a supplementary description of *** from China

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Plant Diversity 2023年第2期45卷 185-198页

作者： Bin Yang Jin-Yue Li Rui-Jie Yang Hong-Bo Ding Min Deng Chun-Fen Xiao yun-Juan zuo yun-Hong Tan Southeast Asia Biodiversity Research Institute Chinese Academy of Sciences&Center for Integrative ConservationXishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303YunnanChina The Key Laboratory of Rare and Endangered Forest Plants of National Forestry and Grassland Administration&The Key Laboratory for Silviculture and Forest Resources Development of yunnan Province Yunnan Academy of Forestry and GrasslandKunming 650201YunnanChina College of Life Science University of Chinese Academy of SciencesHuairou DistrictBeijing 101408China School of Ecology and Environmental Science Yunnan UniversityKunming 650091YunnanChina Center for Gardening and Horticulture Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303YunnanChina

Two new species of Polyalthiopsis(Annonaceae),*** *** & Bin Yang from Guangxi and yunnan Provinces and *** *** & Bin Yang from yunnan Province,are described and *** is morphologically similar to *** in having narrowly elliptic-oblong,lemon to yellowish green petals,but differs by having obovoid monocarps,a higher number of leaf secondary veins,leaf blades usually widest above the middle,and a lower ratio of leaf blade length to *** is morphologically similar to *** in having axillary inflorescences,1-3(-4) flowers,elliptic leaves,and ellipticovate petals,but differs in the numbers of carpels per flower and ovules per *** molecular phylogenetic analysis using five plastid markers confirm that the two new species belong to the genus Polyalthiopsis and show clear interspecific divergences between *** and *** and between them and other species in the *** descriptions,colored photographs,and habitat and distribution data for the two new species are *** addition,the fruit morphology of *** is described for the first time,based on living *** distributions and a diagnostic key for all Polyalthiopsis species are also presented.

关键词： Annonaceae Morphology Polyalthia Polyalthiopsis Southeast yunnan

p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B

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BIOCELL 2019年第4期43卷 321-326页

作者： HEYA QIAN YAN YAN ZHENGJIE SHEN LIXIAN XU yun zuo TAO ZHU YANAN CHEN Department of Oncology the Affiliated Zhangjiagang Hospital of Soochow UniversityZhangjiagang215600China Department of Pharmacology the Affiliated Zhangjiagang Hospital of Soochow UniversityZhangjiagang215600China Department of Laboratory the Affiliated Zhangjiagang Hospital of Soochow UniversityZhangjiagang215600China

The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells.U2OS cells were treated with siRNA against p53,Rap2B,and PLCε.Relative expressions of p53,Rap2B,and PLCεwere determined using quantitative polymerase chain reaction(qPCR)and Western blotting,*** of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay(ELISA).Levels of Ca^(2+) were detected using Flow *** microscopy was used to observe the autophagy of *** of p53 significantly decreased the expressions of Rap2B ***,knockdown of p53 significantly decreased the mRNA levels of PLCε.The knockdown of p53,Rap2B,and PLCεsignificantly decreased the levels of intracellular IP3 and Ca^(2+) and promoted autophagy of U2OS *** results demonstrated that p53-Rap2B-PLCε-IP3 signaling pathway regulated autophagy of U2OS cells.

关键词： PLCε IP3 Ca^(2+) Autophagy

Knockout of three aminopeptidase N genes does not affect susceptibility of Helicoverpa armigera larvae to Bacillus thuringiensis Cry1A and Cry2A toxins

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Insect Science 2020年第3期27卷 440-448页

作者： Jing Wang Ya-yun zuo Ling-Li Li Hui Wang Shao-Yan Liu Yi-Hua Yang Yi-Dong Wu College of Plant Protection Nanjing Agricultural UniversityNanjingChina

Bacillus thuringiensis(Bt)insecticidal toxins have been globally utilized for control of agricultural insects through spraying or transgenic *** of Bt toxins to special receptors on midgut epithelial cells of target insects is a key step in the mode of *** studies suggested aminopeptidase N1(APN1)as a receptor or putative receptor in several lepidopteran insects including Helicoverpa armigera through evidence from RNA interefence‐based gene silencing *** the current study we tested the role of APNs in the mode of action of Bt toxins using clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR‐associated protein 9‐mediated gene *** APN genes(HaAPN1,HaAPN2 and HaAPN5)were individually knocked out in a susceptible strain(SCD)of *** to establish three homozygous knockout *** in vitro binding studies indicated binding of Cry1Ac or Cry2Ab to midgut brush border membrane vesicles was not obviously affected by APN *** results showed that none of the three knockouts had significant changes in susceptibility to Cry1A or Cry2A toxins when compared with the SCD *** suggests that the three HaAPN genes we tested may not be critical in the mode of action of Cry1A or Cry2A toxins in ***.

关键词： aminopeptidase N Bacillus thuringiensis CRISPR/Cas9 Helicoverpa armigera receptor

High Serum Resistin Level may be an Indicator of the Severity of Coronary Disease in Acute Coronary Syndrome

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Chinese Medical Sciences Journal 2009年第3期24卷 161-166页

作者： Hao Wang De-you Chen Jian Cao zuo-yun He Bing-po Zhu Min Long Department of Geriatric Cardiology Out-patient Department Chinese People＇s Liberation Army General Hospital Beijing 100853 China Department of Internal Cardiology Department of Endocrinology Xinqiao Hospital the Third Military Medical University Chongqing 400037 China

Objective To investigate the correlation between serum resistin level, cardiovascular risk factors and severity of coronary disease in acute coronary syndrome （ACS）. Methods Alter evaluated by clinical history, electrocardiography, exercise tolerance tests, laboratory tests, and coronary angiography, 220 consecutive patients with suspected chest pain were divided into normal control group, stable angina pectoris （SAP） group, and ACS group, respectively. Baseline clinical characteristics, including height, weight, waist circumference, hip circumference, white blood cell count, high-sensitive C-reactive protein （hsCRP）, total cholesterol, triglyceride, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol, were compared among three groups. ELISA was used to detect serum resistin levels. Pearson＇s correlation coefficient analysis was used to assess association between resistin and other traditional cardiovascular risk factors. Multinomial logistic regression analyses were used to define the relationship between serum resistin level and SAP or ACS. Results Serum resistin level in ACS group （1.18±0.48 μg/L） was significantly higher than that in normal control and SAP groups （0.49±0.40 and 0.66±0.40 μg/L; P〈0.01）. Only in ACS group, increased serum resistin level was significantly correlated with hsCRP （r=0.262, P=0.004） and white blood cell count （r=0.347, P=0.001）. Furthermore, serum resistin levels showed a stepwise increase with the number increase of 〉 50% stenosed coronary vessels. Multinomial logistic regression test demonstrated that serum resistin was a strong risk factor for ACS （OR=29.132, 95 % CI： 10.939-77.581, P〈0.001）. Conclusion These findings suggested the potential role of resistin in atherosclerosis and especially its involvement in ACS.

关键词： resistin atherosclerosis acute coronary syndrome stable angina pectoris