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2,3,7,8-Tetrachlorodibenzo-p-dioxin Promotes Proliferation of Astrocyte Cells via the Akt/STAT3/Cyclin D1 Pathway

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Biomedical and Environmental Sciences 2019年第4期32卷 281-290页

作者： WU Chang Yue YIN Kai Zhi ZHANG Yan JIAO Man ZHAO Xin Yuan WU qi yun Medical Colledge Nantong University Department of Nutrition and Food Hygiene School of Public HealthNantong University Xishan District Center for Disease Control and Preventive Department of Occupational Medicine and Environmental Toxicology School of Public HealthNantong University

Objective The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD), a persistent organic pollutant, is harmful to the nervous system, but its effects on the brain are still unclear. This study aimed to investigate the effects of TCDD on astrocytes proliferation and underlying molecular mechanism. Methods The cell proliferation was measured by EdU-based proliferation assay and PI staining by flow cytometry. Protein expression levels were detected by Western blotting. Immunofluorescence, cytoplasmic and nuclear fractions separation were used to assess the distribution of signal transducer and activator of transcription 3(STAT3). Results C6 cells treated with 10 and 50 nmol/L TCDD for 24 h showed significant promotion of the proliferation of. The exposure to TCDD resulted in the upregulation in the expression levels of phosphorylated protein kinase B(p-Akt), phosphorylated STAT3, and cyclin D1 in a dose-and time-dependent manner. The inhibition of Akt expression with LY294002 or STAT3 expression with AG490 abolished the TCDD-induced cyclin D1 upregulation and cell proliferation. Furthermore, LY294002 suppressed the activation of STAT3. Finally, TCDD promoted the translocation of STAT3 from the cytoplasm to the nucleus, and LY294002 treatment blocked this effect. Conclusion TCDD exposure promotes the proliferation of astrocyte cells via the Akt/STAT3/cyclin D1 pathway, leading to astrogliosis.

关键词： 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) Akt STAT3 Cyclin D1 Proliferation Astrocytes

注射用益气复脉(冻干)激活PI3K/Akt通路改善小鼠心肌缺血再灌注损伤的机制研究

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临床和实验医学杂志 2024年第9期23卷 902-906页

作者：刘超权陈运起郑梅凡姚亚男海南西部中心医院心血管内科海南儋州571799

目的研究注射用益气复脉(冻干)激活磷脂酰肌醇-3-激酶/丝苏氨酸蛋白激酶(PI3K/Akt)通路对小鼠心肌缺血再灌注损伤(MIRI)的改善机制。方法将56只健康雄性小鼠按照随机数字表法分为4组:假手术组(n=8)、缺血-再灌注(I/R)组(n=16)、缺血-再... 详细信息

目的研究注射用益气复脉(冻干)激活磷脂酰肌醇-3-激酶/丝苏氨酸蛋白激酶(PI3K/Akt)通路对小鼠心肌缺血再灌注损伤(MIRI)的改善机制。方法将56只健康雄性小鼠按照随机数字表法分为4组:假手术组(n=8)、缺血-再灌注(I/R)组(n=16)、缺血-再灌注+注射益气复脉(冻干)组(I/R+YQFM组)(n=16)、缺血-再灌注+注射益气复脉(冻干)组+PI3K/Akt信号通路抑制剂Wortmannin注射液组(I/R+YQFM+Wort组)(n=16)。假手术组小鼠穿线但不结扎冠状动脉,等待缺血25 min后通过腹腔缓慢注射0.9%氯化钠溶液2 mL/kg。I/R组小鼠结扎前降支缺血25 min后通过腹腔缓慢注射0.9%氯化钠溶液2 mL/kg,5 min后再灌注。I/R+YQFM组小鼠同I/R组处理,仅将0.9%氯化钠溶液换为注射益气复脉(冻干)0.867 g/kg。I/R+YQFM+Wort组小鼠术前30 min经腹腔缓慢注射Wortmannin注射液1 mg/kg,其余处理同I/R+YQFM组。记录各组小鼠心脏血流动力学指标[左室舒张末内腔容积(LVESV)、左心室短轴缩短率(FS)、射血分数(EF)],并测算心肌梗死面积。采用蛋白质印迹法分析抗磷酸化Akt(p-Akt)、总Akt(t-Akt)、磷酸化糖原合成酶激酶3β(p-GSK3β)、总GSK3β(t-GSK3β)蛋白表达水平。结果I/R 24 h后,I/R组、I/R+YQFM组、I/R+YQFM+Wort组的LVESV均大于假手术组,FS、EF均小于假手术组,差异均有统计学意义(P0.05)。I/R 24 h后,I/R组、I/R+YQFM+Wort组、I/R+YQFM组的p-Akt、p-GSK3β、p-Akt/t-Akt和p-GSK3β/t-GSK3β蛋白表达水平均高于假手术组,差异均有统计学意义(P<0.05);I/R+YQFM组的p-Akt、p-GSK3β、p-Akt/t-Akt和p-GSK3β/t-GSK3β蛋白表达水平均高于I/R组,差异均有统计学意义(P<0.05);I/R+YQFM+Wort组的上述蛋白表达水平均低于I/R+YQFM组,差异均有统计学意义(P<0.05)。结论注射用益气复脉(冻干)可有效改善小鼠MIRI引起的心脏损伤,这可能与激活PI3K/Akt通路抑制心肌细胞凋亡有关。

关键词：小鼠心肌再灌注损伤益气复脉凋亡 PI3K/Akt通路

Fiber Grating Sensor with Enhanced Pressure and Temperature Sensitivity

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Chinese Physics Letters 2000年第2期17卷 115-116页

作者： LIU yun-qi GUO Zhuan-yun LIU Zhi-Guo ZHAO Dong-Hui GE Chun-Feng DONG Xiao-Yi Institute of Modern Optics Nankai UniversityTianjin 300071

A technique is used to enhance the pressure and temperature sensitivity of a fiber Bragg grating *** grating is packaged by using polymer jacket,which exhibits no significant chirp due to the adoption of the special *** measured pressure and temperature sensitivity of the structured grating is 6.28×10^(-5)/MPa and5.18×10^(-5)/℃.The wavelength shift due to pressure and temperature can be enhanced about 31.5 times for pressure and 7.7 times for temperature.

关键词： technique temperature fiber

A FUNCTIONAL CONJUGATED HYPERBRANCHED POLYMER DERIVED FROM TETRAPHENYLETHENE AND OXADIAZOLE MOIETIES:SYNTHESIS BY ONE-POT “A_4+B_2+C_2” POLYMERIZATION AND APPLICAION AS EXPLOSIVE CHEMOSENSOR AND PLED

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Chinese Journal of Polymer Science 2013年第10期31卷 1432-1442页

作者： Wen-bo Wu Shang-hui Ye Li-jin Huang Gui Yu yun-qi Liu Jin-gui qin Zhen Lia Department of Chemistry Hubei Key Lab on Organic and Polymeric Opto-Electronic MaterialsWuhan University Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences

A new conjugated hyperbranched polymer （hb-TFO） containing tetraphenylethylene （TPE） units, a famous aggregation-induced emission （AIE） active group, as the core, was synthesized successfully with modest yield via one-pot Suzuki polymerization reaction. Thanks to the introduction of TPE moieties, hb-TFO exhibited aggregation-enhanced emission （AEE） property, and could work as explosive chemosensor with high sensitivity. The polymeric light-emitting diode （PLED） device was fabricated to investigate its electroluminescent property, and hb-TFO demonstrated a maximum luminance efficiency of 0.22 cd/A and a maximum brightness of 545 cd/m2 at 15.9 V.

关键词： Hyperbranched polymer AEE TPE Explosive chemosensor PLED.

嫩江洋闭合时限:来自早二叠世大乌苏岩体锆石年代学和地球化学的制约

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地质与资源 2024年第3期33卷 267-279页

作者：马维马婧轩洪阳百合文韵琪辽宁省第六地质大队有限责任公司辽宁大连116200 东北大学资源与土木工程学院地质系辽宁沈阳110819 中国科学技术大学地球和空间科学学院安徽合肥230026

嫩江洋作为古亚洲洋东段的重要分支,其闭合时限对限定中亚造山带东段的构造演化具有重要意义.对大兴安岭北部大乌苏地区出露的二叠纪岩体进行了岩石学、年代学和地球化学研究,探讨其构造产出背景,进而为约束嫩江洋的闭合时限及大兴安岭... 详细信息

嫩江洋作为古亚洲洋东段的重要分支,其闭合时限对限定中亚造山带东段的构造演化具有重要意义.对大兴安岭北部大乌苏地区出露的二叠纪岩体进行了岩石学、年代学和地球化学研究,探讨其构造产出背景,进而为约束嫩江洋的闭合时限及大兴安岭北部地区构造演化提供新的证据.锆石U-Pb年代学结果显示,二长花岗岩加权平均年龄为292±4 Ma,形成于早二叠世.岩石属弱过铝质I型花岗岩,样品稀土分配模式曲线呈右倾型,大离子亲石元素Rb、Ba、K富集,高场强元素Nb、P、Ti亏损,呈现后造山花岗岩特征,可能形成于伸展环境.大乌苏二长花岗岩具有中等程度的Eu负异常(δEu=0.46~0.47),Th/U比值为5.61~5.94,Nb/Ta比值为7.98~8.14,Ti/Zr比值为6.11~6.36,且Mg^(#)值(22~23)较低.上述地球化学特征暗示其应形成于下地壳的部分熔融,在上升过程中发生了程度较高的结晶分异作用.综合区域上已发表的年代学资料,将区内石炭纪—二叠纪岩浆作用划分为360~350 Ma、345~292 Ma、289~260 Ma三期.通过对比各期岩浆岩的时空分布、岩石组合和地球化学特征,认为上述三期岩浆岩呈现了俯冲—碰撞后—造山后伸展的连续变化特点,记录了嫩江洋的闭合过程.研究认为大乌苏二长花岗岩应属于第二期岩浆作用的产物,产于额尔古纳-兴安地块与松嫩地块碰撞后的伸展环境,由此限定嫩江洋应闭合于早石炭世末期.

关键词：嫩江洋早二叠世地质年代学地球化学大乌苏地区大兴安岭

Nitrogen in soils beneath 18 65 year old stands of subtropical evergreen broad-leaved forests in Laoshan Mountains in Eastern China

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Journal of Forestry Research 2011年第4期22卷 583-590页

作者： GU Feng ZHANG Kai ZHANG yun-qi WANG qin XU Xiao-niu Department of Forest Science Anhui Agricultural University Hefei 230036 China

Monitoring of soil nitrogen （N） cycling is useful to assess soil quality and to gauge the sustainability of management practices. We studied net N mineralization, nitrification, and soil N availability in the 0-10 cm and 11-30 cm soil horizons in east China during 2006-2007 using an in situ incubation method in four subtropical evergreen broad-leaved forest stands aged 18-, 36-, 48-, and 65-years. The properties of surface soil and forest floor varied between stand age classes. C：N ratios of surface soil and forest floor decreased, whereas soil total N and total organic C, available P, and soil microbial biomass N increased with stand age. The mineral N pool was small for the young stand and large for the older stands. NO3^--N was less than 30% in all stands. Net rates of N mineralization and nitrification were higher in old stands than jn younger stands, and higher in the 0-10 cm than in the 11-30 cm horizon. The differences were significant between old and young stands （p 〈 0.031） and between soil horizons （p 〈 0.005）. Relative nitrification was somewhat low in all forest stands and declined with stand age. N trans- formation seemed to be controlled by soil moisture, soil mierobial biomass N, and forest floor C：N ratio. Our results demonstrate that analyses of N cycling can provide insight into the effects of management disturbances on forest ecosystems.

关键词： microbial biomass nitrogen nitrogen availability nitrogenmineralization soil horizon subtropical forest

清肺排毒汤、化湿败毒方和宣肺败毒方体外抗补体活化作用的比较研究

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中药药理与临床 2024年第7期40卷 36-41页

作者：李西蒙藏晨晨郭怡琳蔡敏闫晶晶李文静刘壮壮高源齐云中国医学科学院北京协和医学院药用植物研究所北京100193

目的:平行比较新冠肺炎“抗疫三方”(清肺排毒汤、化湿败毒方和宣肺败毒方)对抗新冠肺炎(COVID-19)重要病机补体活化的作用。方法:基于酶联免疫吸附测定(ELISA)原理,采用免疫球蛋白M(IgM)、酵母多糖和甘露聚糖作为包被物分别启动经典途... 详细信息

目的:平行比较新冠肺炎“抗疫三方”(清肺排毒汤、化湿败毒方和宣肺败毒方)对抗新冠肺炎(COVID-19)重要病机补体活化的作用。方法:基于酶联免疫吸附测定(ELISA)原理,采用免疫球蛋白M(IgM)、酵母多糖和甘露聚糖作为包被物分别启动经典途径、替代途径和凝集素途径,通过测定补体活化终端产物攻膜复合物(C5b-9)水平以评价补体活化程度。不仅如此,通过包被新冠病毒刺突蛋白(S蛋白)与核衣壳蛋白(N蛋白)还可考察“三方”对抗这两种蛋白引起补体活化的作用。结果:“三方”均能剂量依赖地对抗经典途径、替代途径和凝集素途径引起的C5b-9生成,也均对S蛋白与N蛋白引起的C5b-9生成具有抑制作用,其中宣肺败毒方的作用最强。结论:“三方”均可在体外对抗三条补体途径的激活并抑制新冠S蛋白与N蛋白引起的补体活化,此作用可能是三方发挥治疗COVID-19作用的药效学基础之一。

关键词：清肺排毒汤化湿败毒方宣肺败毒方补体活化新冠肺炎补体活化终端产物攻膜复合物刺突蛋白核衣壳蛋白

Small RNA deep sequencing reveals full-length genome of Citrus yellow vein clearing virus in Chongqing,China

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Journal of Integrative Agriculture 2017年第2期16卷 503-508页

作者： YU yun-qi WU qiong SU Hua-nan WANG Xue-feng CAO Meng-ji ZHOU Chang-yong College of Plant Protection Southwest University National Citrus Engineering and Technology Research Center Citrus Research InstituteSouthwest University/Chinese Academy of Agricultural Sciences National Navel Orange Engineering and Technology Research Center Gannan Normal University

To identity the potential pathogen associated with the yellow vein clearing symptom on lemon trees, the profiles of virus-de- rived small interfering RNAs from citrus samples were obtained and analyzed by deep sequencing method in this study. Twenty-seven contigs almost cover the full length genome of Citrus yellow vein clearing virus （CYVCV） isolate YN were obtained using the small RNA deep sequencing technology. Analysis showed that this isolate CQ shared the highest nucleotide identity with isolate Y1 （JX040635） and YN （KP313242）, both of which belong to the genus Mandarivirus in the family Alphaflexiviridae. Mapping analysis of viral-derived siRNA （vsiRNA） profiles revealed an uneven distribution pattern of their generations along both positive and negative genome strands, and 22- and 21-nt vsiRNAs ranked the majority. BLAST against viroids and other viral databases confirmed that this sample was single-infected only by CYVCV, which indicated that CYVCV was the exact causal agent for the yellow clearing symptom occurring on lemon. This is the first CYVCV isolate detected in Chongqing and the second in China. This result could provide a molecular basis for the investigation of citrus viral diseases to protect citrus health in this region.

关键词： citrus Citrus yellow vein clearing virus (CYVCV) small RNA deep sequencing

Differential expressions among five Waxy alleles and their effects on the eating and cooking qualities in specialty rice cultivars

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Journal of Integrative Agriculture 2015年第6期14卷 1153-1162页

作者： ZHOU Li-jie SHENG Wen-tao WU Jun ZHANG Chang-quan LIU qiao-quan DENG qi-yun Longping Branch of Graduate School Central South University State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center Key Laboratories of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics Ministry of Education/Agricultural College Yangzhou University

Eating and cooking qualities（ECQs） of rice are important attributes due to its major influence on consumer acceptability. To better understand the molecular mechanism of the variation in ECQs, we investigated and compared the expressions among different alleles of the Waxy（Wx） gene and its effect on ECQs in specialty rice cultivars. The results showed that the accumulation of amylose was positively and significantly correlated to the level of mature Wx m RNA and granule-bound starch synthase I（GBSS I） in developing rice grain at 12 days after flowering. The amount of GBSS I and its activity together are the main factors controlling amylose synthesis. Differences in ECQs among five Wx allele types were investigated in samples from 15 rice varieties. The apparent amylose content（AAC） and gel consistency（GC） were similar in each type of Wx allele. The AAC followed the order, Wx^a type〉Wx^in type〉Wx^b type〉Wx^mq type〉wx. Contrary to this, the GC showed an opposite trend compared to AAC. There was a wide variation in rapid visco analyzer（RVA） profile among five Wx allele types, while varieties sharing a specified Wx allele had basically the similar RVA profile, although there was a slight difference in some RVA parameters, peak, hot paste and cool paste viscosities.

关键词： alleles cooking eating qualities GBSS starch specialty flowering mature paste