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钻爆法公路隧道防排水技术发展综述

公路交通科技 2024年第6期41卷 156-164页

作者：晏长根欧运起李富春尹培杰来弘鹏长安大学公路学院陕西西安710064 河北省交通规划设计研究院有限公司河北石家庄050011

为研究钻爆法公路隧道防排水技术的发展趋势和未来方向,系统梳理了防排水技术的发展历程、技术要求、设计施工和维护技术等内容,并对需要关注的问题及下一步发展方向进行展望。结果表明:按照隧道建设的技术发展水平,防排水技术可划分为... 详细信息

为研究钻爆法公路隧道防排水技术的发展趋势和未来方向,系统梳理了防排水技术的发展历程、技术要求、设计施工和维护技术等内容,并对需要关注的问题及下一步发展方向进行展望。结果表明:按照隧道建设的技术发展水平,防排水技术可划分为圬工时代和后混凝土时代,已实现原则指导、设计优化、材料革新、施工控制、标准保障及运营维护的基本构架;中国在新修订的规范中增补了隧道渗漏水量化标准,使防水等级规定更具客观性、科学性;从防排水系统构造形式的对比情况看,控制排水型隧道能兼顾隧道衬砌水压力和维护隧道区生态平衡等多方面需求;钻爆法隧道防排水技术虽然增加了一些可维护性设计,但仍以功能性设计为主,且现有技术难以满足防排水系统的维护需求。应当注意:防排水技术是一个系统性问题,需要建立基于全寿命周期的建管养一体化防排水体系,并对防排水系统选型和控制型防排水技术进行系统性研究,通过对全寿命周期内隧道排水的环境影响进行分析评价,确定合理的防排水构造型式;另外,需加强防排水系统优化及新工艺研究,加快新型防排水材料的开发;同时要加强维护技术研究,尤其是可维护性技术和修复性技术研究。

关键词：隧道工程防排水技术综述防排水系统构造形式维护技术

基于猪细小病毒6型VP1蛋白间接ELISA抗体检测方法的建立及应用

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农业生物技术学报 2024年第6期32卷 1462-1470页

作者：欧云文潘琴汪洋代军飞任绍科张洋张杰开江县动物疫病预防控制中心中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室河北科技师范学院河北省预防兽医学重点实验室

猪细小病毒6型(Porcine parvovirus type 6, PPV6)是一种新发现的猪细小病毒，可导致猪(Sus scrofa)出现繁殖障碍，严重威胁养猪业健康发展。为建立一种检测PPV6抗体的间接ELISA方法，本研究以PPV6流行毒株高度保守的病毒颗粒蛋白(virio... 详细信息

猪细小病毒6型(Porcine parvovirus type 6, PPV6)是一种新发现的猪细小病毒，可导致猪(Sus scrofa)出现繁殖障碍，严重威胁养猪业健康发展。为建立一种检测PPV6抗体的间接ELISA方法，本研究以PPV6流行毒株高度保守的病毒颗粒蛋白(virion protein, VP1)(348～675 aa)的编码基因为目标片段，构建原核表达载体pET30a-PPV6-VP1，转入大肠杆菌(Escherichia coli) BL21 (DE3)感受态细胞，IPTG诱导表达，进行Ni-NTA树脂亲和层析纯化，获得具有良好的反应原性的重组VP1蛋白，大小约为40 k D。基于纯化的重组VP1蛋白建立间接ELISA抗体检测方法，优化出最佳抗原包被浓度为1 ng/μL，待检血清最佳稀释比例为1∶100,HRP-兔抗猪IgG稀释比例为1∶60 000，阳性临界值OD450>0.62。该间接ELISA检测方法与猪蓝耳病毒(Porcine reproductive and respiratory syndrome virus, PRRSV)、古典猪瘟病毒(Classical swine fever virus, CSFV)、猪伪狂犬病毒(Pseudorabies virus, PRV)、日本乙型脑炎病毒(Japanese encephalitis virus, JEV)、猪圆环病毒2型(Porcine circovirus type 2, PCV2)、PPV1阳性猪血清均不发生交叉反应，敏感度达1∶3 200，批内变异系数和批间变异系数均<10%，与病毒中和试验(virus neutralization test, VNT)结果呈正相关，与Western blot结果的阳性符合率为96.6%，表明该间接ELISA检测方法拥有良好的特异性、敏感性、重复性和符合率。采用建立的间接ELISA方法对2018～2022年中国西部地区部分省份收集的452份猪血清进行检测，结果显示样品阳性率为5.31%，表明该病在我国西部地区存在不同程度的感染。本研究成功截短表达了PPV6 VP1 (348～675 aa)蛋白，建立了特异性强、灵敏度高、重复性好和符合率高的PPV6间接ELISA方法，为PPV6的血清学调查和检测试剂盒的开发提供了材料。

关键词：猪细小病毒6型(PPV6) VP1蛋白截短表达 ELISA

2022年达州市家畜O型口蹄疫免疫抗体监测与分析

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动物医学进展 2024年第2期45卷 123-127页

作者：张蕾欧云文刘俐君赵小波唐慧伦杨青李成高嵩李清竹达州市动物疫病预防控制中心四川达州635000 开江县动物疫病预防控制中心四川达州636250 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃兰州730046

为准确掌握2022年达州市家畜O型口蹄疫(Foot-and-mouth disease, FMD)免疫效果,从辖区180个场(点)随机采集猪、牛和羊血清2 879份,采用酶联免疫吸附试验(ELISA)进行O型FMD免疫抗体检测。结果显示,家畜O型FMD免疫抗体场(点)阳性率为88.8... 详细信息

为准确掌握2022年达州市家畜O型口蹄疫(Foot-and-mouth disease, FMD)免疫效果,从辖区180个场(点)随机采集猪、牛和羊血清2 879份,采用酶联免疫吸附试验(ELISA)进行O型FMD免疫抗体检测。结果显示,家畜O型FMD免疫抗体场(点)阳性率为88.89%,样品阳性率为88.78%,均达到农业农村部规定标准。在不同种类家畜方面,牛场(点)阳性率和样品阳性率均最高,分别为93.94%和93.41%;在场(点)类型方面,屠宰环节的场(点)阳性率显著低于养殖环节(P<0.05),规模场(存栏量≥500头/只)的场(点)阳性率、样品阳性率均高于适度规模场(存栏量<500头/只)和散养户;在不同区域方面,通川区场(点)阳性率和样品阳性率均最高,渠县最低;在不同季节方面,场(点)阳性率最高为夏季(92.31%),而样品阳性率最高为冬季(90.76%);从不同生长阶段来看,种畜场(点)阳性率和样品阳性率均最高,分别为96.72%和92.62%。监测结果表明,达州市家畜O型FMD免疫效果较好,建立了有效的免疫保护屏障,可为进一步做好FMD科学防控提供数据参考。

关键词：家畜 O型口蹄疫免疫抗体

Inverse Coefficient Problems for Nonlinear Elliptic Variational Inequalities

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Acta Mathematicae Applicatae Sinica 2011年第1期27卷 85-92页

作者： Run-sheng Yang yun-hua ou Department of Mathematics Changsha University of Sciences and Technology Changsha 410076 China Department of Mathematics Hunan University of Technology Zhuzhou 412007 China

This paper is devoted to a class of inverse coefficient problems for nonlinear elliptic variational inequalities. The unknown coefficient of elliptic variational inequalities depends on the gradient of the solution and belongs to a set of admissible coefficients. It is shown that the nonlinear elliptic variational inequalities is unique solvable for the given class of coefficients. The existence of quasisolutions of the inverse problems is obtained.

关键词： Elliptic variational inequality inverse coefficient problem existence of quasisolution

Clinical characteristics and pre-surgery diagnostic criteria of aldosterone-producing adrenocortical carcinoma

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Chinese Medical Journal 2022年第20期135卷 2512-2514页

作者： Xinyi Zhang Qingguo Lyu Jinfang Ma Xiaohui Pan Qiang Wei Jianwei Li Yuchun Zhu Sumin Shen Jing Li yun ou Nanwei Tong Department of Endocrinology and Metabolism Sichuan University West China HospitalChengduSichuan 610041China Center for Diabetes and Metabolism Research Sichuan University West China HospitalChengduSichuan 610041China Department of Urology Sichuan University West China HospitalChengduSichuan 610041China Department of Pathology Sichuan University West China HospitalChengduSichuan 610041China

To the Editor:Primary aldosteronism,characterized by hypertension,with or without hypokalemia,is the most frequent form of endocrine hypertension,which is mostly caused by bilateral adrenal hyperplasia or aldosterone-producing adrenocortical adenomas(APAA).However,it can also be induced by aldosterone-producing adrenocortical carcinoma(APAC),which is *** of the rarity and malignancy.

关键词： aldosterone hypertension surgery

Inverse Coefficient Problems for Nonlinear Parabolic Differential Equations

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Acta Mathematica Sinica,English Series 2008年第10期24卷 1617-1624页

作者： yun Hua ou Alemdar HASANOV Zhen Hai LIU Department of Mathematics Hu'nan University of Technology Zhuzhou Hu'nan 412007 P. R. China Applied Mathematical Sciences Research Center Kocaeli University Izmit-Kocaeli 41300 Turkey Department of Mathematics Central South University Changsha 410075 P. R. China

This paper is devoted to a class of inverse problems for a nonlinear parabolic differential equation. The unknown coefficient of the equation depends on the gradient of the solution and belongs to a set of admissible coefficients. It is proved that the convergence of solutions for the corresponding direct problems continuously depends on the coefficient convergence. Based on this result the existence of a quasisolution of the inverse problem is obtained in the appropriate class of admissible coefficients.

关键词： Parabolic equations Inverse coefficient problems Existence of quasisolutions

Analysis of large datasets for identifying molecular targets in intestinal polyps and metabolic disorders

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BIOCELL 2024年第3期48卷 415-429页

作者： SHAN ou yun XU QINGLAN LIU TIANWEN YANG WEI CHEN XIU YUAN XIN ZUO PENG SHI JIE YAO Department of Gastroenterology The First Affiliated Hospital of Chongqing Medical and Pharmaceutical CollegeChongqingChina

Background:The interrelation between intestinal polyps,metabolic syndrome(MetS),and colorectal cancer(CRC)is a critical area of *** research focuses on pinpointing potential molecular targets to understand the link between intestinal polyp formation,metabolic irregularities,and CRC ***:We examined clinical samples from patients with intestinal polyps coexisting with MetS and compared them with samples from patients with standard intestinal *** sequencing and public database analysis were employed to identify significant pathways and *** targets were then validated through immunohistochemistry(IHC).Following the RNA interference of key target expression,a series of experiments,including the cell counting kit-8 assay,colony formation,wound healing,and Transwell assays,were ***:Comparative analysis revealed 75 up-regulated and 61 down-regulated differentially expressed genes(DEGs)in the MetS polyp group *** *** encyclopedia of genes and genomes(KEGG)pathway enrichment suggested these DEGs were primarily associated with cell cycle and *** with comparative toxicogenomics database(CTD)and the cancer genome atlas(TCGA)databases highlighted 44 key CRC-related *** interaction networks indicated connections of purkinje cell protein 4(PCP4),olfactomedin 1(OLFM1),fibronectin 1(FN1),and transforming growth factor beta 3(TGF-β3)with the mitogen-activated protein kinase(MAPK)*** correlation studies suggested higher risk associations with FN1,PCP4,and TGF-β3,while OLFM1 was identified as a lower risk *** analysis revealed a decrease in OLFM1 in MetS-associated intestinal *** interference with OLFM1 in polyp epithelial cells,there was a significant enhancement in cell proliferation,colony formation,and cell migration and invasion ***:our study highlights a significant decrease in OLFM1 expression in MetS-associated intestinal

关键词： Colorectal cancer Metabolic syndrome Intestinal polyp OLFM1 Exosome

Quantitative nanoscale mapping of three-phase thermal conductivities in filled skutterudites via scanning thermal microscopy

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National Science Review 2018年第1期5卷 59-69页

作者： Ehsan Nasr Esfahani Feiyue Ma Shanyu Wang yun ou Jihui Yang Jiangyu Li Department of Mechanical Engineering University of Washington Shenzhen Key Laboratory of Nanobiomechanics Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences Department of Materials Science andEngineering University of Washington

In the last two decades, a nano structuring paradigm has been successfully applied in a wide range of thermoelectric materials, resulting in significant reduction in thermal conductivity and superior thermoelectric performance. These advances, however, have been accomplished without directly investigating the local thermoelectric properties, even though local electric current can be mapped with high spatial resolution. In fact, there still lacks an effective method that links the macroscopic thermoelectric performance to the local micro structures and properties. Here, we show that local thermal conductivity can be mapped quantitatively with good accuracy, nanometer resolution and one-to-one correspondence to the micro structure using a three-phase skutterudite as a model system. Scanning thermal microscopy combined with finite element simulations demonstrate close correlation between sample conductivity and probe resistance,enabling us to distinguish thermal conductivities spanning orders of magnitude, yet resolving thermal variation across a phase interface with small contrast. The technique thus provides a powerful tool to correlate local thermal conductivities, micro structures and macroscopic properties for nano structured materials in general and nano structured thermo electrics in particular.

关键词： thermal conductivity scanning thermal microscopy thermoelectric materials nanoscale heat transfer thermal imaging

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整本书深度阅读教学实践探究

山西财经大学学报 2023年第S1期45卷 172-174页

作者：欧运波曲靖师范学院教师教育学院云南曲靖655000

整本书深度阅读既是体现整本书阅读价值的基础,也是培养学生深度学习能力和提升学生语文核心素养的重要途径。针对整本书阅读实践中存在的阅读浅表化、指导粗放化、评价单一化等问题,应通过读书报告会、指导精细化、评价多元化等措施来... 详细信息

整本书深度阅读既是体现整本书阅读价值的基础,也是培养学生深度学习能力和提升学生语文核心素养的重要途径。针对整本书阅读实践中存在的阅读浅表化、指导粗放化、评价单一化等问题,应通过读书报告会、指导精细化、评价多元化等措施来解决,进而推进整本书深度阅读。

关键词：整本书阅读深度阅读教学实践