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Synchronous nanoscale topographic and chemical mapping by differential-confocal controlled Raman microscopy

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Photonics Research 2020年第9期8卷 1441-1447页

作者： HAN CUI yun WANG liRONG QIU SHUCHENG li JONATHAN M.COOPER AND WEIQIAN ZHAO Key Laboratory for Precision Optoelectronic Measurement Instrument and Technology School of Optics and PhotonicsBeijing Institute of TechnologyBeijing 100081China Division of Biomedical Engineering James Watt School of EngineeringUniversity of GlasgowGlasgow G128LTUK

Confocal Raman microscopy is currently used for label-free optical sensing and imaging within the biological,engineering,and physical sciences as well as in industry.However,currently these methods have limitations,including their low spatial resolution and poor focus stability,that restrict the breadth of new applications.This paper now introduces differential-confocal controlled Raman microscopy as a technique that fuses differential confocal microscopy and Raman spectroscopy,enabling the point-to-point collection of three-dimensional nanoscale topographic information with the simultaneous reconstruction of corresponding chemical information.The microscope collects the scattered Raman light together with the Rayleigh light,both as Rayleigh scattered and reflected light(these are normally filtered out in conventional confocal Raman systems).Inherent in the design of the instrument is a significant improvement in the axial focusing resolution of topographical features in the image(to^1 nm),which,when coupled with super-resolution image restoration,gives a lateral resolution of 220 nm.By using differential confocal imaging for controlling the Raman imaging,the system presents a significant enhancement of the focusing and measurement accuracy,precision,and stability(with an antidrift capability),mitigating against both thermal and vibrational artefacts.We also demonstrate an improved scan speed,arising as a consequence of the nonaxial scanning mode.

关键词： scattered chemical stability

Genetic characteristics of a patient with multiple primary cancers:A case report

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World Journal of Clinical Cases 2021年第28期9卷 8563-8570页

作者： Wei-Wei Ouyang Qing-yun li Wen-Gang Yang Sheng-Fa Su li-Jia Wu Ying Yang Bing Lu Department of Thoracic Oncology The Affiliated Hospital of Guizhou Medical University and Cancer Hospital of Guizhou Medical UniversityGuiyang 550000Guizhou ProvinceChina Genecast Biotechnology Co. LtdWuxi 214104Jiangsu ProvinceChina

BACKGROUND Two or multiple primary malignant neoplasms(MPMNs)rarely occur in the same patient.It has been reported that MPMNs are easily misdiagnosed as the recurrence or metastasis of malignancies in clinical practice,affecting the choice of treatment for the patients,thereby resulting in the delay of optimal diagnosis.Next generation sequencing(NGS)can be used to distinguish between multiple primary lung cancers and intrapulmonary metastasis,and may distinguish the origin of tumours in different sites of the body.CASE SUMMARY We report the case of 66-year-old woman who suffered from different malignant neoplasms in the rectum and esophageal and gastrointestinal tract.The first neoplasm rectal adenocarcinoma was diagnosed and removed in 2016.The second and third lesions were diagnosed with esophageal squamous-cell carcinoma(ESCC)and gastrointestinal stromal tumour(GIST),respectively,in 2019.Nextgeneration whole exome sequencing was performed on the tissue specimens of rectal carcinoma,esophageal cancer,GIST,and white blood cells to investigate the relationship between malignancies at different timeframe and determine whether the ESCC and GIST evolved from the rectal adenocarcinoma.Mutations including v-Ki-ras2-Kirsten rat sarcoma viral oncogene homolog,adenomatosis polyposis coli,and mothers against decapentaplegic homolog 4 were detected in rectal adenocarcinoma sample,mast/stem cell growth factor receptor was detected in GIST tissue,and lysine methyltransferase 2D was detected in ESCC specimen.Overall,ESCC and GIST were not genetically evolved from rectal adenocarcinoma,and this patient did not have a trunk driven clone.CONCLUSION NGS is an effective tool to study clonal evolution of tumours and distinguish between MPMNs and intrapulmonary metastasis.

关键词： Multiple primary malignant neoplasms Whole exome sequencing Rectal carcinoma Esophageal squamous-cell carcinoma Gastrointestinal stromal tumour Case report

QTL Mapping for Wheat Flour Color with Additive,Epistatic,and QTL×Environmental Interaction Effects

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Agricultural Sciences in China 2011年第5期10卷 651-660页

作者： li Wei-hua liU Wei liU li YOU Min-shan liU Guang-tian li Bao-yun Key Laboratory of Oasis Eco-Agriculture of Xinjiang Production and Construction Group Shihezi University Shihezi 832003 P.R. China Key Laboratory of Crop Genomics ＆ Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100193 P.R. China

To investigate genetic factors affecting wheat flour color traits,a linkage map was constructed using a recombinant inbred line （RIL） population derived from Jing 771×Pm 97034.Main,epistatic and QTL×environment （QE） interaction effects of quantitative trait loci （QTLs） controlling wheat flour color were studied by the mixed linear modeling of data collected from wheat RIL plants under three different environmental conditions.13 QTLs with additive effects and 55 pairs of QTLs with epistatic effects were detected for wheat flour color traits.The additive-additive interactions （AA） involved all of the wheat chromosomes except 3D.Epistasis accounted for more of the observed phenotypic variation than did the main effect QTLs （M-QTLs）.Our results suggested that dual-locus interactions are widespread in the wheat genome and play a critical role in determining wheat flour color characteristics.In this study,3 QTLs were identified to have QE interaction effects,one of them showing significant QE interaction in E2 environment.

关键词： epistatic effects flour color QTL×environment effects quantitative trait loci Triticum aestivum

Comparison of the veracity of real-time perfusion, harmonic angio, and ultraharmonic myocardial contrast imaging modes in evaluation of acute myocardial infarction area

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Chinese Medical Journal 2006年第3期119卷 179-184页

作者： XING Yan-qiu ZHANG yun li Da-qing liU Xiang-qun li Xiu-qin ZHAO Chang-qin CHEN Mei GAO Hai-qing Cardiology Unit of Senior Care Department Qilu HospitalShandong University Ji＇nan 250012 China

Background Innovative advancements in ultrasound instrumentation present a number of imaging modalities for myocardial contrast echocardiography （MCE） in ischemic syndromes. How well they compare to each other in diagnostic accuracy in the detection of acute myocardial infarction is unclear. The purpose of this study was to assess the relative accuracy of 3 different imaging modes of MCE, low mechanical index （MI） real-time perfusion imaging （RTPI）, triggered harmonic angio mode （HA）, and ultraharmonic imaging mode （UH） in the detection of acute experimental myocardial infarction within the time frame suitable for potential reperfusion. Methods MCE was performed in 10 open-chest dogs using RTPI, triggered HA and triggered UH modes at baseline and one hour after occlusion of left anterior descending coronary artery. Presence or absence of peffusion defects, and the perfusion defect size when present, were analyzed and compared with the infarct size delineated by triphenyltetrazolium chloride （TTC） staining. Results The infarct area was （15.8-2.4）% by TTC staining; Peffusion defect area by MCE was similar to anatomic infarct area in all the three MCE approaches：（16.1-2.7）% by RTPI mode, （15.5-2.9）% by HA mode, and （15.5-3.0）% by UH mode. The sensitivity, specificity and overall diagnostic accuracy in the detection of myocardial infarction were 100%, 88%, and 94% for RTPI mode, 88%, 100%, and 94 % for HA mode, and 100%, 75%, and 88% for UH mode. Conclusion All modes of MCE, RTPI, triggered HA mode and triggered UH mode have excellent diagnostic accuracy in the immediate hour of acute coronary occlusion within the optimal time frame suitable for reperfusion therapy.

关键词： myocardial contrast echocardiography myocardial infarction ultraharmonic imaging harmonic angio imaging real-time perfusion imaging

A new data normalization method for unsupervised anomaly intrusion detection

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第10期11卷 778-784页

作者： Long-zheng CAI Jian CHEN yun KE Yao CHEN Zhi-gang li Engineering and Commerce College South-Central University for Nationalities Wuhan 430065 China Guangdong Institute of Science and Technology Zhuhai 519090 China

Unsupervised anomaly detection can detect attacks without the need for clean or labeled training data.This paper studies the application of clustering to unsupervised anomaly detection(ACUAD).Data records are mapped to a feature space.Anomalies are detected by determining which points lie in the sparse regions of the feature space.A critical element for this method to be effective is the definition of the distance function between data records.We propose a unified normalization distance framework for records with numeric and nominal features mixed data.A heuristic method that computes the distance for nominal features is proposed,taking advantage of an important characteristic of nominal features-their probability distribution.Then,robust methods are proposed for mapping numeric features and computing their distance,these being able to tolerate the impact of the value difference in scale and diversification among features,and outliers introduced by intrusions.Empirical experiments with the KDD 1999 dataset showed that ACUAD can detect intrusions with relatively low false alarm rates compared with other approaches.

关键词： Unsupervised anomaly detection Data mining Intrusion detection Network security

Preliminary experience of hybrid endoscopic submucosal dissection by duodenoscope for recurrent laterally spreading papillary lesions

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World Journal of Gastroenterology 2020年第37期26卷 5673-5681页

作者： Zi-Kai Wang Fang liu yun Wang Xiang-Dong Wang Ping Tang Wen li Department of Gastroenterology and Hepatology The First Medical CenterChinese PLA General HospitalBeijing 100853China Department of Pathology The First Medical CenterChinese PLA General HospitalBeijing 100853BeijingChina

BACKGROUND The management strategies for recurrent ampullary adenoma after endoscopic papillectomy are still controversial.Patients with the recurrent papillary lesions need to receive repetitive endoscopic interventions due to the limitations of conventional endoscopic techniques.AIM To assess the feasibility,efficacy,and safety of hybrid endoscopic submucosal dissection(ESD)by duodenoscope for recurrent,laterally spreading papillary lesions.METHODS We enrolled two patients with recurrent,laterally spreading,duodenal papillary adenomas with no intraductal extension confirmed by follow-up between March 2017 and September 2018.After marking the resection borders of the lesion using a dual knife,a submucosal cushion was created by injecting a mixture of saline solution,methylene blue,and adrenaline.A total circumferential incision and submucosal excision was performed by dual knife combined with insulated-tip diathermic knife,and then the lesion was ligated and resected using an electric snare.Endoscopic hemostasis was applied during the endoscopic procedures.Moreover,the endoscopic retrograde cholangiopancreatography(ERCP)procedures,including selective cannulation and stent implantation of biliary and pancreatic ducts,were performed.Additionally,we performed endoclip closure for mucosal defect after ESD.RESULTS Hybrid ESD using a duodenoscope and biliary and pancreatic stent placement were performed successfully in two patients.The endoscopic size of recurrent papillary lesions was no more than 2 cm.Generally,the average total procedure time was 95.5 min,and the procedure time of ESD and ERCP was 38.5 min and 15.5 min,respectively.No serious complications occurred during the intraoperative and postoperative periods.The histopathological examination revealed tubulovillous adenoma negative for neoplastic extension at the cut margin in both patients.The duodenoscopic follow-up and histopathology of biopsy specimens at 3 mo after ESD showed no residual or recurrent lesions in ampullary areas in both cases.Both cases h

关键词： Endoscopic submucosal dissection Ampullary adenoma Recurrent Laterally spreading Papillary lesions

Predominant expression of Th1-type cytokines in primary hepatic cancer and adjacent liver tissues

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Hepatobiliary & Pancreatic Diseases International 2007年第1期6卷 63-66页

作者： Qiu, Fa-Bo Wu, li-Qun Lu, yun Zhang, Shun Zhang, Bing-Yuan Qingdao Univ Affiliated Hosp Dept Hepatobiliary Vasc Surg Qingdao 266003 Peoples R China

BACKGROUND: Research has revealed a shift towards Th2 in many types of malignant tumor, but the state of Th1/Th2 is not clear in patients with primary hepatic cancer (PHC). This study was designed to determine the expression of Th1- versus Th2-type cytokines in primary hepatic cancer and the adjacent liver tissue in order to provide evidence for treatment of the Th1/Th2 shift. METHODS: Samples were collected from 11 patients with PHC. The gene expression of Th1/Th2 cytokines was detected by reverse transcriptase polymerase chain reaction (RT-PCR) using IFN-gamma and IL-2 as Th1-type cytokine genes, and IL-4 and IL-10 as Th2-type cytokine genes. RESULTS: Th1-type cytokines were expressed in 7/11 PHCs and 9/11 adjacent liver tissues, while Th0 type cytokines occurred in 4/11 PHCs and 2/11 adjacent liver tissues. CONCLUSION: Th1-type cytokines are expressed predominantly in primary hepatic cancer and the adjacent liver tissue.

关键词： primary hepatic carcinoma cytokine gene expression reverse transcriptase polymerase chain reaction

Hydrothermal Synthesis and Crystal Structure of a Cadmium(II) Polymer with One-dimensional Chain Structure: [Cd(bpy)(BDC)]_n·nbpy

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Chinese Journal of Structural Chemistry 2007年第5期26卷 537-540页

作者： li Xiu-Mei NIU Yan-ling WANG Qing-Wei CUI yun-Cheng liUBo Department of Chemistry Tonghua Teachers College Tonghua Jilin 134002 China Jilin Normal University Siping Jilin 136000 China

A metal-organic coordination polymer [Cd（bpy）（BDC）]n·nbpy （bpy = 2,2-bipyri-dine, H2BDC = terephthalic acid） has been hydrothermally synthesized and structurally characterized by elemental analysis, IR spectrum, TG and single-crystal X-ray diffraction. The complex crystallizes in monoclinic, space group C2/c with a = 15.723（5）, b = 21.695（5）, c = 7.576（5） A, β = 116.171（7）°, V = 2319.3（18）A^3, C28H20CdN4O4, Mr = 588.88, Dc = 1.686 g/cm^3, μ（MoKα） = 0.987 mm^-1, F（000） = 1184, Z = 4, the final R = 0.0464 and wR = 0.0831 for 1882 observed reflections （Ⅰ〉 2σ（Ⅰ））. It exhibits a three-dimensional network with channels constructed from one-dimensional coordination chains via C–H…O hydrogen bonds and significant aromatic π-π stacking interactions.

关键词： hydrothermal synthesis crystal structure coordination polymer

Thermal,Mechanical and Rheological Properties of Biodegradable Poly(propylene carbonate) and Poly(butylene carbonate) Blends

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Chinese Journal of Polymer Science 2015年第3期33卷 444-455页

作者： Dan-dan Wu Wu li Yan Zhao yun-jiao Deng 张会良 Hui-xuan Zhang li-song Dong Changchun University of Technology Laboratory of Polymer Ecomaterials Chinese Academy of SciencesChangchun Institute of Applied Chemistry

Poly（propylene carbonate）（PPC） was melt blended in a batch mixer with poly（butylene carbonate）（PBC） in an effort to improve the toughness of the PPC without compromising its biodegradability and biocompatibility. DMA results showed that the PPC/PBC blends were an immiscible two-phase system. With the increase in PBC content, the PPC/PBC blends showed decreased tensile strength, however, the elongation at break was increased to 230% for the 50/50 PPC/PBC blend. From the tensile strength experiments, the Pukanszky model gave credit to the modest interfacial adhesion between PPC and PBC, although PPC/PBC was immscible. The impact strength increased significantly which indicated the toughening effects of the PBC on PPC. SEM examination showed that cavitation and shear yielding were the major toughening mechanisms in the blends subjected the impact tests. TGA measurements showed that the thermal stability of PPC decreased with the incorporation of PBC. Rheological investigation demonstrated that the addition of PBC reduced the value of storage modulus, loss modulus and complex viscosity of the PPC/PBC blends to some extent. Moreover, the addition of PBC was found to increase the processability of PPC in extrusion. The introduction of PBC provided an efficient and novel toughened method to extend the application area of PPC.

关键词： Biodegradable Poly（propylene carbonate） Poly（butylene carbonate） Blends,