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Screening and Phylogenetic Analysis of Deep-Sea Bacteria Capable of Metabolizing Lignin-Derived Aromatic Compounds

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Open Journal of Marine Science 2012年第4期2卷 177-187页

作者： Y. Ohta S. nishi T. Haga T. Tsubouchi R. Hasegawa M. Konishi Y. nagano Y. Tsuruwaka Y. Shimane K. Mori K. Usui E. Suda K. Tsutsui A. nishimoto Y. Fujiwara T. Maruyama Y. Hatada Institute of Biogeosciences Japan Agency of Marine-Earth Science and Technology (JAMSTEC) Yokosuka Japan

Lignin is one of the most abundant biomasses in nature. It is composed of aromatic moieties and has great potential for use in the production of chemical alternatives to petroleum products. Because of increasing interest in biocatalysis, the potential for industrial application of microbial metabolism of lignin-derived compounds has gained considerable recent attention. Functional screenings of culturable bacteria isolated from sediments and sunken wood collected from the deep sea revealed the existence of a number of previously unidentified bacteria capable of metabolizing lignin-related aromatic compounds. Of the 510 isolates obtained in the present study, 208 completely or partially metabolized these compounds. The 208 isolates were classified into diverse phyla, including Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria. Among the 208 isolates, 61 unique 16S rRnA gene sequences were detected including previously unidentified marine lineage isolates. The metabolites of the isolates were analysed using liquid chromatography/mass spectrometry (LC/MS) or gas chromatography/mass spectrometry (GC/MS). Most of the representative 61 isolates non-oxidatively decarboxylated the substrates to produce the corresponding aromatic vinyl monomers, which are used as feed stocks for bio-based plastics production. Oxidative metabolism of the lignin-related compounds for assimilation was frequently observed. Our study showed that the deep-sea environment contains an abundance of microorganisms capable of both non-oxidative and oxidative bioconversion of lignin-derived aromatic compounds. The ability for bio-conversion of aromatic compounds found in this study will facilitate the development of future biotechnological applications.

关键词： Aromatic Compound Bacteria Deep Sea Lignin

The thymidylate kinase gene homologues identified from various jujube witches'-broom phytoplasma isolates infecting different jujube cultivars in China

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The thymidylate kinase gene homologues identified from vario...

第三届中国森林保护学术大会

作者： Qicong Xu~(1,2) Guozhong Tian~(1**) Caili Lin~1 Yong Li~1 Chungen Piao~1 1 Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China 2 International nature Farming Research Center,5632 Hata,nagano 390-1401,Japan

Thymidylate kinase(TMK,EC 2.***.4.9) that catalyses the transfer of a terminal phosphoryl group from ATP to dTMP,is crucial to both the de novo synthetic and the salvage pathways for pyrimidine *** types of thymidylate kinase genes,tmkX,tmkY and tmkZ(TMK gene),of the phytoplasma associated with jujube witches’-broom disease(JWB) were identified by means of sequence analyses as well as RFLP and SSCP of the specific PCR products in thirty four JWB infected jujube samples collected from different cultivars with diverse geographic origins and resistance to the JWB in *** and tmkY gene sequences were identified in many jujube cultivars from different regions, whereas tmkZ was only found in BJLZ strain infecting an aged dragon jujube from Beijing,which contained tmkX sequence as *** and tmkY have the closest relationship with tmk-a gene (AB010446) in OY-W strain of onion yellows and tmkZ have the closest relationship with one of tmk gene copies in genome of OY-M(AP006628,from 391588 to 392202).Amino acid sequence analyses showed that all the three deduced proteins TMKx,TMKy and TMKz of tmkX,tmkY and tmkZ contained conserved motifs for catalytic *** collected JWB isolates inhabiting different resistanct cultivars against the phytoplasma could be mainly classified into three types,typeX,typeY and typeXY,containing tmkX gene, tmkY gene and both tmkX and tmkY genes,*** portion of typeXY strains were detected than typeX and Y from the collection of JWB *** with mere typeX or typeY sequeces seem to be frequenctly in association with susceptible cultivars,while the typeXY strain was likely to be much related to resistant *** is assumed that the formation and distribution of different tmk genotype clusters among JWB strain population might be related to the phytoplasmal gene duplication and the selection by the infected cultivars and geographical environment as well as the gene recombination due to

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大气492GHz亚毫米波不透明度的测量

天文学报 2004年第1期45卷 79-87页

作者：杨戟孙成华黄树频大石雅寿宫泽敬辅中国科学院紫金山天文台南京210008 日本国立天文台野边山宇宙电波观测所

使用移动式亚毫米波望远镜(POST)在位于青藏高原海拔3200米的紫金山天文台德令哈射电天文观测站址测量地球大气492GHz频率处天顶方向的不透明度((?)0)的结果.在1999-2000年冬季和2000-2001年冬季的两个观测季节内累计共进行了约870个小... 详细信息

使用移动式亚毫米波望远镜(POST)在位于青藏高原海拔3200米的紫金山天文台德令哈射电天文观测站址测量地球大气492GHz频率处天顶方向的不透明度((?)0)的结果.在1999-2000年冬季和2000-2001年冬季的两个观测季节内累计共进行了约870个小时的测量,取得了25842组(?)0的有效数据.对数据的统计表明,观测季节内(?)0值主要分布在1.5-3.0之间.观测时段内大气不透明度(?)0<1.0的时间比例约占3%.在给出实测资料的基础上,将所测量的亚毫米波不透明度与国际现有亚毫米波台址的不透明度进行了初步比较.

关键词：测量亚毫米波大气不透明度选址

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水平圆管内高普朗特数流体的凝固

石油学报 1985年第4期 87-99页

作者：菱田干雄长野靖尚孙元名古屋工业大学教授石油工业部管道设计院助理工程师

本文从高普朗特数流体所具有的共同特性出发,用数值计算方法对在壁温一定的水平圆管内、伴随着非稳态自然对流换热的高普朗特数流体的凝固过程进行了解析。从而使管内发生的自然对流的流动样相随时间之推移的变化、液固两相非稳态温度... 详细信息

本文从高普朗特数流体所具有的共同特性出发,用数值计算方法对在壁温一定的水平圆管内、伴随着非稳态自然对流换热的高普朗特数流体的凝固过程进行了解析。从而使管内发生的自然对流的流动样相随时间之推移的变化、液固两相非稳态温度分布与自然对流换热的关系、凝固界面的形状及其随时间的变化、自然对流对于凝固速度的影响等原来所不清楚的问题得以明确。

关键词：水平圆管壁温普朗特数非稳态表面温度对流传热自然对流换热高温流体液相温度

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野鸦椿酯类化合物抗炎症活性与结构的研究

天然产物研究与开发 2004年第4期16卷 290-293页

作者：董玫张秋霞广田满河北医科大学病理生理教研室石家庄050017 信州大学农学部生理活性化学教室

从野鸦椿 (EuscaphisjaponecaKantiz)枝叶的甲醇提取物中分离得到 3个结构相似的酯类化合物 ,通过波谱分析鉴定为 7 hydroxy 2 octen 5 olide (Euscapholide ,1) ,3,7 dihydroxy 5 octanolide (2 ) ,和methyl 5 ,7 dihydroxy 2 (Z) octenoate (3)。化合物 1和 3具有较强的抗炎症活性 ,而结构相似的化合物 2却不显示抗炎症活性 ;通过对这些化合物及诱导体的抗炎症活性的测定 ,初次确定野鸦椿酯类化合物的抗炎症活性与其结构中的α、β不饱和羰基密切相关。

关键词：省沽油科野鸦椿酯类化合物炎症植物化学结构环氧酶

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原发性恶性胆总管周围神经鞘瘤

世界核心医学期刊文摘（胃肠病学分册） 2005年第9期1卷 51-51页

作者： MatsuoK.-I. nagano Y. SugimoriK. 陈琳 Gastroenterological Center Yokohama City University Medical Center Division of Pathology 4-57 Urafune-cho Minamiku Yokohama 232-0024 Japan

Primary malignant peripheral nerve-sheath tumors of the common bile duct are extremely rare. To our knowledge, the published literature contains no previous case report of this disease. Here we report on a 58-year-old Japanese woman with a primary malignant peripheral nerve-sheath tumor of the common bile duct, which was completely resected. A hypoechoic mass was identified in the hepatic hilus, using ultrasonography and computed tomography. Endoscopic retrograde cholangiography revealed a smooth stricture and deviation of the common bile duct. Laparotomy exposed a firm mass around the common bile duct that had not invaded the surrounding tissues. Partial resection of the common bile duct and cholecystectomy were performed as the treatment of choice. The final histopathological diagnosis was malignant peripheral nerve-sheath tumor arising from the wall of the common bile duct.

关键词：神经鞘瘤胆总管肝门胆囊切除术逆行胆管造影低回声团块剖腹探查术病理学诊断女性病例文献报道

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新生儿心输出血量的脑分布

世界核心医学期刊文摘（儿科学分册） 2005年第9期1卷 10-10页

作者： Kusaka T. Okubo K. nagano K. 宁亮 Maternal Perinatal Center Faculty of Medicine Kagawa University 1750-1 Mikicha Kitagun Kagawa 761-0793 Japan Dr.

Cerebral blood flow and output of the left ventricle were simultaneously investigated in 17 infants using multichannel near infrared spectroscopy and pulse dye densitometry with indocyanine green. Cardiac output and cerebral blood flow were positively related. The control of cardiac output is important in the regulation of cerebral blood flow in infants.

关键词：左心室输出量脑血流量靛氰绿心输出量密度测定法红外分光光谱检测

帕金森病的脑萎缩及其与认知能力损害的相关性

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世界核心医学期刊文摘（神经病学分册） 2005年第6期1卷 40-40页

作者： nagano- Saito A. Washimi Y. Arahata Y. 赵睿 Department of neurology Natl. Ctr. Geriat. and Gerontology 36- 3 Gengo Morioka Obu Aichi 474- 8511 Japan Dr.

Objective: Voxel- based morphometry was used to compare the amounts of gray matter in the brains of patients with Parkinson disease (PD) and normal control subjects (nCs) and to identify the specific regions responsible for cognitive dysfunction in PD. Methods: Patients were classified into nondemented (nD) and demented (D) groups according to the criteria of the Diagnostic and Statistical Manual of Mental Disorders (4th ed.), and a group comparison was performed. In the nD patients, a correlation was also performed between local gray matter density and the score on Raven Colored Progressive Matrices (RCPM), a test of executive and visuospatial function. Results: In patients with advanced nD- PD vs nCs, atrophic changes were observed in the limbic/paralimbic areas and the prefrontal cortex. In D vs nD patients, atrophic change was observed widely in the limbic/paralimbic system, including the anterior cingulate gyrus and hippocampus as well as the temporal lobe, dorsolateral prefrontal cortex, thalamus, and caudate nucleus. The RCPM score was positively correlated with the gray matter density in the dorsolateral prefrontal cortex and the parahippocampal gyrus. Conclusions: In patients with Parkinson disease (PD), atrophic changes occur mainly in the limbic/paralimbic and prefrontal areas. These atrophic changes may be related to the development of dementia in PD.

关键词：帕金森病脑萎缩痴呆组认知障碍外侧部形态测量学前额皮质尾状核海马区边缘区

华支睾吸虫重组半胱氨酸蛋白酶用于血清学调查的效果评价

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中国病原生物学杂志 2008年第8期3卷 598-600页

作者：裴福全长野功方悦怡崔惠儿吴军阮彩文广东省疾病预防控制中心寄生虫病防治研究所广东广州510300 日本岐阜大学医学部寄生虫学研究室

目的以华支睾吸虫重组半胱氨酸蛋白酶(CsCp)为ELISA包被抗原检测流行人群血清特异性抗体水平,与成虫粗抗原比较,评价其用于华支睾吸虫病血清学调查的价值。方法以佛山市南海区的1个行政村为调查点,用改良加藤厚涂片法检查粪便华支睾吸... 详细信息

目的以华支睾吸虫重组半胱氨酸蛋白酶(CsCp)为ELISA包被抗原检测流行人群血清特异性抗体水平,与成虫粗抗原比较,评价其用于华支睾吸虫病血清学调查的价值。方法以佛山市南海区的1个行政村为调查点,用改良加藤厚涂片法检查粪便华支睾吸虫虫卵。同时采集血清,分别以rCsCP与成虫粗抗原(CsCAA)的ELISA方法检测血清特异性抗体水平,对粪检及两种抗原的ELISA结果进行统计学分析。结果接受血清检测的97人中,华支睾吸虫虫卵阳性率60.8%,CsCAA-ELISA阳性率75.3%,rCsCP-ELISA阳性率76.3%,特异性抗体阳性率高于虫卵阳性率(P0.05);CsCAA-ELISA、rCsCP-ELISA结果与粪检结果的符合率分别为56.7%和55.7%,两种抗原ELISA结果的符合率为82.5%。59例粪检阳性者CsCAA-ELISA和rCsCP-ELISA阳性率均为76.3%,38例粪检阴性者CsCAA-ELISA和rCsCP-ELISA阳性率分别为73.7%和76.3%。结论以rCsCP为包被抗原,用ELISA检测华支睾吸虫病流行区人群血清特异性IgG抗体与使用CsCAA的检测结果有较高的符合率,但同样不能区分现症与既往感染。

关键词：华支睾吸虫重组半胱氨酸蛋白酶血清学调查