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Systematic genome editing of the genes spanning an entire chromosome by CRISPR/Cas9 in a vertebrate——zebrafish (Danio rerio)

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Science China(Life Sciences) 2020年第7期63卷 1096-1097页

作者： yulong gong Bingyuan Yang Wenbiao Chen Department of Molecular Physiology&Biophysics Vanderbilt University School of MedicineNashvilleTN 37232USA State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China

Deciphering the biological functions of each gene in the genome is fundamental for understanding the molecular mechanisms underlying normal development, physiology,and behavior, as well as diseases. One common approach to determine gene function is to disrupt individual genes and assess the consequences. Zebrafish (Danio rerio) has been gaining popularity as a model organism to analyze gene function. This is particularly true in China.

关键词： Danio rerio Systematic genome editing of the genes spanning an entire chromosome by CRISPR/Cas9 in a vertebrate zebrafish CRISPR

Depletion of insulin receptors leads to β-cell hyperplasia in zebrafish

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Science Bulletin 2017年第7期62卷 486-492页

作者： Binyuan Yang Gang Zhai yulong gong Jingzhi Su Dong Han Zhan Yin Shouqi Xie State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China University of the Chinese Academy of Sciences Beijing 100049 China Freshwater Aquaculture Collaborative Innovation Center of Hubei Province Wuhan 430070 China

Hyperglycemia in type 2 diabetes results from an inability of insulin to regulate *** characterize the role of the insulin/insulin receptor pathway in glycometabolism and type 2 diabetes,we created a zebrafish model in which insulin receptors a and b(insra and insrb) have been *** first observed that insra and insrb were both expressed abundantly during embryonic development and in various adult *** expression of insulin and number of b-cells were observed in insra-/-/-insrb-/-fish together with higher glucose in insra-/-,insrb-/-,or insra-/-/-insrb-/-fish,indicating that insra and insrb were knocked out ***,compared to the wild-type fish,insra-/-/-insrb-/-fish died between 5 and 16 days post-fertilization(dpf) with severe pericardial edema and increased level of cell apoptosis,which was not induced by increased total body glucose *** gluconeogenesis and decreased glycolysis were also observed in both single and double knockout fish,but no mortality or malformation was observed in single knockout *** the importance of insulin receptors in glucose homeostasis and embryonic development,transcriptome analysis was used to provide an important model of defective insulin signaling and to study its developmental consequences in *** results indicated that both insra and insrb played a pivotal role in glucose metabolism and embryonic development,and insra was more critical than insrb in the insulin signaling pathway.

关键词： Hyperglycemia Type 2 diabetes Zebrafish β-cells Insulin receptors

Effects of fish meal replacement with Chlorella meal on growth performance,pigmentation,and liver health of largemouth bass(Micropterus salmoides)

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Animal Nutrition 2022年第3期10卷 26-40页

作者： Longwei Xi Qisheng Lu yulong Liu Jingzhi Su Wen Chen yulong gong Dong Han Yunxia Yang Zhimin Zhang Junyan Jin Haokun Liu Xiaoming Zhu Shouqi Xie State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed Wuhan 430072China The Innovative Academy of Seed Design Chinese Academy of SciencesWuhan 430072China

Chlorella meal is a potential protein source for ***,the physiological response of carnivorous fish fed Chlorella meal remains *** study evaluated the effects of replacing dietary fish meal with Chlorella meal on growth performance,pigmentation,and liver health in largemouth *** diets were formulated to replace dietary fish meal of 0%(C0,control),25%(C25),50%(C50),75%(C75),and 100%(C100)with Chlorella meal,*** 300 fish(17.6±0.03 g)were randomly assigned to 15 tanks(3 tanks/group).Fish were fed the experimental diet twice daily for 8 *** increased dietary Chlorella meal quadratically influenced the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and feed intake(FI),which were significantly lower in the C100 group than in the other groups(P<0.05).The feed conversion ratio(FCR)increased linearly or quadratically with dietary Chlorella *** Chlorella meal linearly or quadratically increased the lutein content of plasma,liver,and dorsal muscle of largemouth bass(P<0.05).Compared to the C0 group,all supplemented Chlorella meal groups significantly improved the yellowness(b*)of the dorsal body(1.5 to 2.0 fold),abdominal body(1.5 to 1.8 fold),and dorsal muscle(3.8 to 5.4 fold)of largemouth bass(P<0.05).In addition,compared to the C0 group,the liver vacuolation area of fish was significantly increased in the C75 and C100 groups(P<0.05).Transcriptional levels of apoptosis-related genes of b-cell lymphoma-2(bcl2),caspase-9-like(casp9),and caspase-3a(casp3)were markedly upregulated(0.9 to 1.6 fold)in the C100 group compared to the C0 group(P<0.05).Based on the quadratic regression analysis between FBW,WGR,or SGR and dietary Chlorella meal level,largemouth bass had the best growth when replacing 31.7%to 32.6%of fish meal with 15.03%to 15.43%dietary Chlorella *** present results indicated that dietary supplementation with Chlorella meal(11.85%to 47.45%)significantly enhanced the pigmentation;how

关键词： Chlorella meal Growth Liver health Micropterus salmoides Pigmentation

4-Formyl-2-(2H-benzotriazol-2-yl)-phenol: an ESIPT chromophore

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Chinese Science Bulletin 2014年第34期59卷 4741-4751页

作者： Yao Lu yulong gong Huiying Ma Zhenqiang Wang Haili Li Ziping Luo Hongru Li Fang Gao School of Chemistry and Chemical Engineering Chongqing University

In the present work, excited state intramolecular proton transfer(ESIPT) emission properties of a new benzotriazole derivative 4-formyl-2-(2H-benzotriazol-2-yl)-phenol(C1) were studied. 4-Formyl-2-(2H-benzotriazol-2-yl)-methoxy-benzene(C2), 4-formyl-2-(2H-benzotriazol-2-yl)-phenyl acetate(C3) and 4-methyl-2-(2H-benzotriazol-2-yl)-phenol(C4) were used as the reference molecules.1H NMR chemical shift of hydroxy group in C1 was located at more down field than that of C4 or p-hydroxy-benzaldehyde(C5), respectively. C1 showed two absorption bands in the range of 260–400 nm zones in various solvents, while C2 and C3 exhibited single absorption band. The equal molar mixtures of C2/C5 or C3/C5 showed single absorption band.C2 and C3 displayed single fluorescence emission band in various solvents, while C1 exhibited dual emission bands in some strong polar solvents. Furthermore, the second emission band in these strong polar solvents showed the large Stokes shift. The results show that the second emission band of C1 was produced by ESIPT. C2 and C3 could not undergo ESIPT due to no hydroxy group. The geometry optimization calculation of enol and keto forms in the ground and excited states of C1 provided tough theoretical evidences of ESIPT.

关键词：苯并三唑甲酰基激发态分子内质子转移苯酚极性溶剂生色发射性能三唑衍生物

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考虑表面效应的纳米圆形薄板振动

中国石油大学学报（自然科学版） 2017年第5期41卷 153-158页

作者：赵德敏龚宇龙中国石油大学储运与建筑工程学院山东青岛266580

基于Gurtin的表面弹性理论考虑表面弹性和残余表面应力,建立周边固支的轴对称纳米圆板的振动微分方程。研究残余表面应力对纳米板振动频率和主振型的影响。结果表明:前四阶固有频率分别随着残余表面应力参数的增大而减小;但残余表面应... 详细信息

基于Gurtin的表面弹性理论考虑表面弹性和残余表面应力,建立周边固支的轴对称纳米圆板的振动微分方程。研究残余表面应力对纳米板振动频率和主振型的影响。结果表明:前四阶固有频率分别随着残余表面应力参数的增大而减小;但残余表面应力对一阶固有频率和主振型影响最大,随着振动阶数的增大,残余表面应力对固有频率和主振型的影响逐渐减小;同时对于一阶固有频率,板的厚度越薄或者板的半径越大,受残余表面应力的影响越大。

关键词：纳米圆板振动表面弹性残余表面应力固有频率主振型

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基于语义的微博短文本倾向性分析研究

计算机应用研究 2016年第10期33卷 2914-2918页

作者：马力刘笑宫玉龙西安邮电大学计算机学院西安710061

通过结合情感词库和微博语义特征,采用向量空间模型的方法表示微博文本,提出一种融合模式匹配和机器学习的微博文本倾向性分析方法。对分词之后的微博文本,先提取出情感关键词,再匹配分析出的几种模式抽取情感评价短句、情感短句和微博... 详细信息

通过结合情感词库和微博语义特征,采用向量空间模型的方法表示微博文本,提出一种融合模式匹配和机器学习的微博文本倾向性分析方法。对分词之后的微博文本,先提取出情感关键词,再匹配分析出的几种模式抽取情感评价短句、情感短句和微博表情符号等其他情感特征共同组成情感特征序列,最后采用多步分类的支持向量机方法得到微博文本的情感倾向。通过实验与其他评测结果进行对比,表明该方法是有效的。

关键词：微博情感倾向语义相似度支持向量机

拉西瓦2F、5F机组推力轴承问题分析及处理

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防爆电机 2012年第2期47卷 51-53页

作者：宫玉龙哈尔滨电机厂有限责任公司黑龙江哈尔滨150040

拉西瓦水电站总装机容量为6台700MW水轮发电机,机组自2008年5月首台投入并网使用至今,一直发挥着巨大的作用,在例行检修时发现2F、5F机组推力瓦和镜板出现非正常磨损,通过检修时目击的现象及结合现场实际情况,从整个推力系统入手,综合... 详细信息

拉西瓦水电站总装机容量为6台700MW水轮发电机,机组自2008年5月首台投入并网使用至今,一直发挥着巨大的作用,在例行检修时发现2F、5F机组推力瓦和镜板出现非正常磨损,通过检修时目击的现象及结合现场实际情况,从整个推力系统入手,综合分析了产生这一现象的本质原因,并结合电站检修时间、工具等方面限制的实际情况,采取现场处理镜板、调整喷油管、调用备用推力瓦等工艺方案,圆满的解决了问题,为巨型机组推力轴承系统现场处理积累了经验。

关键词：推力轴承推力瓦镜板喷油管高压油顶起系统油膜厚度