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Microwave-Assisted Synthesis of Poly(Aspartic Acid-Itaconic Acid) Copolymer and Its Characterization

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China Petroleum Processing & Petrochemical Technology 2016年第3期18卷 76-82页

作者： zhang yuling Jiang Yijian Hu Zhiguang Wei Hanxiao Guo Jun Wang Jilong Department of Environmental Science & Engineering North China Electric Power University Department of Computer North China Electric Power University

Poly(aspartic acid-itaconic acid) copolymer was synthesized from aspartic acid(Asp) and itaconic acid(Ita) under microwave irradiation. The effects of microwave power, microwave irradiation time, molar ratio of itaconic acid and aspartic acid, catalyst type, catalyst and organic solvent content on copolymer yield, and the performance for inhibition of CaCO_3 fouling were investigated. It was found that the product yield achieved a highest record of 95% when the amount of catalyst Na H_2PO_4 was 0.012 mol, the amount of organic solvent propylene carbonate was 16 m L, the molar ratio of Asp/Ita was 3:1, the microwave output power was 1200 W and the irradiation time was 5.5 min. And the product performance for inhibition of calcium carbonate also reached a highest value of 94.38%. Structural characterization of the product showed that the product was the aspartic acid-itaconic acid copolymer.

关键词： aspartic acid itaconic acid copolymerization scale inhibition

Common genetic heterogeneity of human interleukin-37 leads to functional variance

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Cellular & Molecular Immunology 2017年第9期14卷 783-791页

作者： Jingjing Yan yuling zhang Shimeng Cheng Bin Kang Jinbiao Peng Xiaodan zhang Meichun Yuan Wenqi Chu Wen zhang Jiayin Shen Shuye zhang Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences Fudan UniversityShanghaiChina School of Life Sciences Hebei Normal UniversityShi-JiazhuangHebei Key Laboratory of Medical Molecular Virology of Ministries of Education/Health Institute of Medical MicrobiologyShanghai Medical College of Fudan UniversityShanghaiChina

Interleukin-37(IL-37)is an inhibitory member of the IL-1 family of *** previously found that balanced selection maintains common variations of the human IL37 ***,the functional consequences of this selection have yet to be ***,using cells expressing exogenous IL-37 variants,including IL-37 Ref and IL-37 Var1 and Var2,we found that the three variants of IL-37 exhibited different immunoregulatory potencies in response to immune *** protein level of IL-37 Var2 was found to be significantly less than that of IL-37 Ref or Var1,despite the comparable mRNA levels of all three *** study showed that IL-37 Var2 was rapidly degraded by a proteasome-dependent mechanism mediated by enhanced polyubiquitination,leading to a transient upregulation of IL-37 Var2 after immune ***,when ectopically expressed in cells,human IL-37 Var2 exerted less inhibition on proinflammatory cytokine production than did other IL-37 ***,purified extracellular IL-37 variant proteins demonstrated comparable inhibitory abilities in *** conclusion,our study reveals that common genetic variants of IL37 lead to different immune-inhibitory potencies,primarily as a result of differences in IL-37 protein stability,suggesting the possible involvement of these variants in various human diseases.

关键词： Cytokine gene variation inflammation protein stability

两种母岩发育森林土壤微生物生物量碳代谢的差异性

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土壤学报 2024年第5期61卷 1432-1443页

作者：付瑞桐万翔宇杨馨逸李德军胡培雷段鹏鹏张玉玲沈阳农业大学土地与环境学院沈阳110866 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室长沙410125 广西自治区重点实验室中国科学院环江喀斯特生态系统观测研究站广西环江547100

针对不同母岩发育土壤的微生物生物量碳代谢特征及驱动因子不明确的科学问题,以石灰岩和碎屑岩两种母岩发育的森林土壤为研究对象,利用18O-H2O标记测定微生物生长速率、微生物呼吸速率、微生物生物量碳利用效率(CUE)以及微生物周转时间... 详细信息

针对不同母岩发育土壤的微生物生物量碳代谢特征及驱动因子不明确的科学问题,以石灰岩和碎屑岩两种母岩发育的森林土壤为研究对象,利用18O-H2O标记测定微生物生长速率、微生物呼吸速率、微生物生物量碳利用效率(CUE)以及微生物周转时间,并结合土壤理化性质、土壤有机质矿物保护特性和土壤酶活性以及微生物生物量和群落组成,明确岩性对森林土壤微生物生物量碳代谢的影响机制。结果表明:石灰岩发育土壤的pH和0.05mm~0.002 mm粒径含量高于碎屑岩发育土壤,而有机碳(SOC)、全氮(TN)、可溶性碳(DOC)、C︰P和N︰P却低于碎屑岩发育土壤(P<0.05);石灰岩发育土壤交换性钙镁(Ca+Mg)和游离态铁铝((Fe+Al)d)含量高于碎屑岩发育土壤,但非晶态铁铝((Fe+Al)o)含量则低于碎屑岩发育土壤;石灰岩发育土壤碳氮磷循环、相关酶活性均显著低于碎屑岩发育土壤(P<0.05);石灰岩发育土壤微生物生物量磷(MBP)高于碎屑岩发育土壤,但微生物生物量碳(MBC)、真菌细菌比(F︰B)和革兰氏阳性菌阴性菌比(G+︰G-)则显著低于碎屑岩发育土壤(P<0.05);石灰岩发育土壤微生物生长速率和周转速率显著高于碎屑岩发育土壤(P<0.05),但微生物呼吸速率和CUE在两种土壤之间差异并不显著。土壤微生物生长速率和微生物周转速率均与土壤pH、(Ca+Mg)︰(Fe+Al)o、(Ca+Mg)︰SOC、(Fe+Al)d︰SOC和革兰氏阴性细菌呈显著正相关(P<0.05),而与DOC、铁铝结合态有机碳、酶活性、MBC︰MBN、F︰B和G+︰G-比呈显著负相关(P<0.05)。此外,土壤CUE与MBC和MBC︰MBN呈显著负相关(P<0.05);微生物呼吸速率仅与酚氧化酶活性呈显著负相关(P<0.05)。两种岩石发育的森林土壤微生物生物量碳代谢受生物和非生物因素的控制,这一研究结果为解释不同母岩发育森林土壤有机碳库的差异提供参考。

关键词：石灰岩碎屑岩森林土壤微生物生物量碳代谢磷脂脂肪酸酶有机质

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An old story with new insight into the structural transformation and radical production of micron-scale zero-valent iron on successive reactivities

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Chinese Chemical Letters 2020年第10期31卷 2634-2640页

作者： Xinhao Wang Xueting Pu Yue Yuan Yunjie Xiang yuling zhang Zhaokun Xiong Gang Yao Bo Lai State Key Laboratory of Hydraulics and Mountain River Engineering College of Architecture and EnvironmentSichuan UniversityChengdu 610065China Sino-German Centre for Water and Health Research Sichuan UniversityChengdu 610065China National Engineering Laboratory for Clean Technology of Leather Manufacture College of Biomass Science and EngineeringSichuan UniversityChengdu 610065China Institute of Environmental Engineering RWTH Aachen UniversityAachen 52062Germany

It is generally recognized that the formation and accumulation of iron oxides on the surface of zero-valent iron(Fe^(0))resulting in significant decrease of contaminant degradation rates during the long-term ***,in this study,we found that the removal efficiencies of p-nitrophenol(PNP)by micro zero-valent iron(mFe^(0))could maintain at the satisfactory level in the process of continuous reactions(20 cycles).The removal rate constant(0.1779 min^(-1))of the 5th cycle was 6.74 times higher than that of the 1streaction(0.0264 min^(-1)),even the 20th cycle(0.0371 min^(-1))was higher than that of the 1st ***,almost no dissolved iron was detected in the solution,and the total iron concentrations decreased dramatically with the process of continuous *** results of scanning electron microscope and energy dispersive spectrometry(SEM-EDS)and X-ray diffraction(XRD)revealed that the structure and composition of corrosion products change from amorphous to highly crystal with the increase of the number of *** corrosion products were mainly magnetite(Fe_(3)O_(4))and a small part of maghemite(γ-Fe_(2)O_(3)),which were in the form of micro sphe res on the surface of mFe^(0).The formation of surface oxidation shell hindered the release of Fe^(2+).X-ray photoelectron spectroscopy(XPS)results illustrated that partial Fe3O_(4) could be converted into y-Fe_(2)O_(3).Electrochemical analysis proved that the electron transfer rate of mFe^(0) increased with the formation of the oxides ***,the consumption of iron core and thicker oxide film weakened the electron transfer ***,the quenching experiments indicated that the reaction activity of mFe^(0) could be enhanced with the addition of *** study deepened the understanding of the structural transformation and radical production of mFe^(0) in continuous reactions.

关键词： Zero-valent iron Iron oxides p-Nitrophenol Structural transformation Successive reactivities

ET-1mRNA反义寡核苷酸治疗模式下糖尿病大鼠生存状况及肾脏病理进展研究

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宁夏医学杂志 2024年第8期46卷 653-655,F0002页

作者：周永忠周晓东张燕张玉玲王凯荣宁夏医科大学基础医学院宁夏银川750004 宁夏医科大学总医院宁夏银川750004 宁夏医药卫生学会服务中心宁夏银川750002

目的对糖尿病肾病(DN)大鼠模型利用内皮素反义寡核苷酸(ET-1AS-ODN)技术进行干预治疗,探讨ET-1AS-ODN在糖尿病肾病(DN)早期肾功能的保护作用。方法使用64只健康的SD雄性大鼠,通过腹腔注射链尿佐菌素(STZ)以剂量为55~60 mg/kg的方法制备... 详细信息

目的对糖尿病肾病(DN)大鼠模型利用内皮素反义寡核苷酸(ET-1AS-ODN)技术进行干预治疗,探讨ET-1AS-ODN在糖尿病肾病(DN)早期肾功能的保护作用。方法使用64只健康的SD雄性大鼠,通过腹腔注射链尿佐菌素(STZ)以剂量为55~60 mg/kg的方法制备糖尿病模型。随后,对其中32只大鼠给予ET-1AS-ODN 6 OD/kg/wk的治疗。在治疗过程中,对大鼠的生存状况及肾功能病理进展情况进行动态观察和测量。结果经过2、4、6、8周实验动物饲养后,与生理盐水对照组相比,8周后模型组(DM)生存率极低(P<0.05),肾功能检测血肌酐(Scr)、尿肌酐、尿素氮(BUN)水平、ET-1含量显著增高。ET-1AS-ODN治疗组在8周后效果显著,能够明显降低DN大鼠的血肌酐(Scr)和尿素氮(BUN)水平,并提高肌酐清除率(Ccr),其差异有统计学意义(P<0.05)。结论经AS-ODN早期干预治疗可以有效改善糖尿病大鼠的生存状态,并对肾功能具有保护作用。

关键词：内皮素-1反义寡核苷酸糖尿病肾病生存状态及肾功能内皮素

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基于纸包装生物基涂层的研究进展

塑料包装 2024年第4期34卷 25-29页

作者：陈新刘俊禧方锴张钰铃赵素芬中山火炬职业技术学院广东中山528436 仲恺农业工程学院广东广州51000

随着人们环保意识的逐渐提高,环保友好、节能减排已成为全球人们关注的重点,在限塑令、禁塑令背景下,以纸代塑是实现环境保护和可持续发展的主要方式。但由于纸基材料表面疏松多孔的结构,小分子的水和气体很容易通过孔隙渗透和润湿纤维... 详细信息

随着人们环保意识的逐渐提高,环保友好、节能减排已成为全球人们关注的重点,在限塑令、禁塑令背景下,以纸代塑是实现环境保护和可持续发展的主要方式。但由于纸基材料表面疏松多孔的结构,小分子的水和气体很容易通过孔隙渗透和润湿纤维,不能满足包装材料的阻隔功能要求,同时纸张没有热封性能,无法通过加热自粘制袋的方式达到工业化生产的要求,且其在机械强度方面难以与塑料相媲美。本文主要从防潮性、阻气性、热封性和机械强度四个角度综述了生物基涂层改性纸基包装材料的研究。

关键词：以纸代塑生物基涂层

基于铜钴硫化物的染料敏化太阳能电池光伏性能的研究

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电子器件 2024年第3期47卷 870-876页

作者：田静吴静马超群潘闻景张宇林淮阴工学院电子信息工程学院江苏省湖泊环境遥感技术工程实验室江苏淮安223003

对电极对染料敏化太阳能电池的性能有很大影响。开发了一种无铂、低成本的二元复合对电极。采用溶剂热法在导电玻璃上成功地制备铜钴硫化物(Cu-Co-S)对电极。以硫化铜对电极为基础,在实验过程中引入钴元素,通过SEM和TEM图像可以看出,钴... 详细信息

对电极对染料敏化太阳能电池的性能有很大影响。开发了一种无铂、低成本的二元复合对电极。采用溶剂热法在导电玻璃上成功地制备铜钴硫化物(Cu-Co-S)对电极。以硫化铜对电极为基础,在实验过程中引入钴元素,通过SEM和TEM图像可以看出,钴元素的引入改变了纯硫化铜对电极单一的物理特性。与此同时,通过Tafel和EIS等电化学测试对铜钴硫化物对电极进行了电催化活性的测试,测试结果表明,钴离子的引入改变硫化铜对电极的晶体结构,同时也提高了对电极的电催化性能。在光电流密度-电压曲线图中可以看到,由硫化铜对电极和铜钴硫化物对电极分别制备的染料敏化太阳能电池的光电转换效率从4.6%提高到5.2%,而铂对电极的原始效率为5.3%,铜钴硫化物对电极的效率已经非常接近于铂电极,从某种程度来说,达到了可以替代铂的作用,故铜钴硫化物对电极有望成为染料敏化太阳能电池更优良的对电极材料。

关键词：染料敏化太阳能电池溶剂热光电转换效率

The Establishment of Infectious Clone and Single Round Infectious Particles for Coxsackievirus A10

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Virologica Sinica 2020年第4期35卷 426-435页

作者： Min Wang Jingjing Yan Liuyao Zhu Meng Wang Lizhen Liu Rui Yu Ming Chen Jingna Xun yuling zhang Zhigang Yi Shuye zhang Shanghai Public Health Clinical Center and Institute of Biomedical Sciences Fudan UniversityShanghai 201508China

Coxsackievirus A10(CVA10)is one of the major etiological agents of hand,foot,and mouth *** are no vaccine and antiviral drugs for controlling CVA10 *** genetic tools for CVA10 will benefit its mechanistic study and development of vaccines and ***,two infectious clones for the prototype and a Myc-tagged CVA10 were *** CVA10 viruses were harvested by transfecting the viral m RNA into human rhabdomyosarcoma(RD)*** CVA10 was further confirmed by next generation sequencing and characterized *** also constructed the vectors for CVA10 subgenomic replicon with luciferase reporter and viral capsid with EGFP reporter,***-transfection of the viral replicon RNA and capsid expresser in human embryonic kidney 293 T(HEK293 T)cells led to the production of single round infectious particles(SRIPs).Based on CVA10 replicon RNA,SRIPs with either the enterovirus A71(EVA71)capsid or the CVA10 capsid were *** by EVA71 SRIPs required SCARB2,while CVA10 SRIPs did ***,we showed great improvement of the replicon activity and SRIPs production by insertion of a cis-active hammerhead ribozyme(HHRib)before the 50-untranslated region(UTR).In summary,reverse genetic tools for prototype strain of CVA10,including both the infectious clone and the SRIPs system,were successfully *** tools will facilitate the basic and translational study of CVA10.

关键词： Coxsackievirus A10(CVA10) Reverse genetics Enterovirus Single round infectious particles(SRIPs) Replicon

基于科普视频的术前访视在腹股沟疝患儿腹腔镜手术中的应用

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中国临床研究 2024年第7期37卷 1135-1138页

作者：陈淑琴黎万琳张玉玲杜淑如周桂兰谢海辉南方医科大学第十附属医院东莞市人民医院手术室广东东莞523200 南方医科大学第十附属医院东莞市人民医院麻醉科广东东莞523200

目的探讨采用科普视频的方式对腹股沟疝患儿进行腹腔镜手术术前访视的效果。方法选取2020年1月至2022年12月东莞市人民医院外科收治的98例拟行腹腔镜手术的腹股沟疝患儿作为研究对象,以入院时间先后分为观察组和常规组,前49例为常规组,... 详细信息

目的探讨采用科普视频的方式对腹股沟疝患儿进行腹腔镜手术术前访视的效果。方法选取2020年1月至2022年12月东莞市人民医院外科收治的98例拟行腹腔镜手术的腹股沟疝患儿作为研究对象,以入院时间先后分为观察组和常规组,前49例为常规组,后49例为观察组。常规组实施传统术前访视,观察组采用基于科普视频的术前访视。测量并比较进入手术室时两组患儿心率、血压、呼吸频率等生理应激变化情况;采用改良耶鲁术前焦虑量表(m-YPAS)、麻醉诱导期合作量表(ICC)评估干预方式对患儿术前不同时间点焦虑程度与麻醉诱导期配合度的影响;比较两组家属的护理满意度。结果观察组进入手术室时心率及呼吸明显加快、血压迅速升高比例低于常规组,等候期及麻醉前术前焦虑程度低于常规组,差异有统计学意义(P<0.05);患儿麻醉诱导期配合度高于常规组(P<0.05);家属护理满意度高于常规组,差异有统计学意义(95.92%vs 73.47%,χ^(2)=9.525,P<0.05)。结论基于科普视频的术前访视有利于减轻行腹腔镜手术腹股沟疝患儿生理应激,缓解焦虑情绪,提升麻醉诱导期配合度,并提高家属对护理方式的满意度。

关键词：术前访视科普视频腹股沟疝腹腔镜手术改良耶鲁术前焦虑量表麻醉诱导