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Nano Research 2022年第6期15卷 5646-5652页

作者： Xiaoyue Qi Qizhe Chen Ziyong Chang yulin deng School of Medical Technology Beijing Institute of TechnologyBeijing 100081China Institute of Engineering Medicine Beijing Institute of TechnologyBeijing 100081China Institute of Chemistry Chinese Academy of SciencesBeijing 100190China Civil and Resource Engineering School University of Science and Technology BeijingBeijing 100083China School of Life Science Beijing Institute of TechnologyBeijing 100081China

Expanding pore size range of metal–organic frameworks(MOFs)promotes their versatility and feasibility for various biomedical ***,natural pore size greatly restricts large guest molecule *** and tailoring pore apertures ranging from micropores to mesopores controllably is desired but still critically ***,we developed a facile method with super mildness based on pH-sensitive zeolitic imidazolate framework(ZIF)-8 to increase porosity,providing pore size with maximum 20 nm,which is 8 times larger than *** oxidase(GOx)was introduced in ZIF-8 for bioetching,benefitted from the resultant acidic microe-nvironment during biocatalytic *** synthesis methods were assessed for obtaining different morphologies and size *** time,GOx encapsulation efficiency,and Zn2+concentration was optimized to precisely control the mesopore size distribution of *** was found that bio-etching strategy was capable of producing stable mesopores which were large enough for loading lactase with good enzymatic activity retained,verified both in vitro and in *** strategy breaks natural pore size limitation of MOFs and thereby facilitates biomolecule delivery,catalysis,and other biomedical applications with enhanced stability and performance.

关键词： metal-organic frameworks enzyme immobilization drug delivery oral biocompatibility

Overview of Biomass Conversion to Electricity and Hydrogen and Recent Developments in Low-Temperature Electrochemical Approaches

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Engineering 2020年第12期6卷 1351-1363页

作者： Wei Liu Congmin Liu Parikshit Gogoi yulin deng School of Chemical and Biomolecular Engineering&Renewable Bioproducts Institute Georgia Institute of TechnologyAtlantaGA 30332-0620USA National Institute of Clean-and-Low-Carbon Energy Beijing 102211China Department of Chemistry Nowgong CollegeNagaon 782001India

Biomass is plant or animal material that stores both chemical and solar energies,and that is widely used for heat production and various industrial *** contains a large amount of the element hydrogen,so it is an excellent source for hydrogen ***,biomass is a sustainable source for electricity or hydrogen *** biomass power plants and reforming plants have been commercialized,it remains a difficult challenge to develop more effective and economic technologies to further improve the conversion efficiency and reduce the environmental impacts in the conversion *** use of biomass-based flow fuel cell technology to directly convert biomass to electricity and the use of electrolysis technology to convert biomass into hydrogen at a low temperature are two new research areas that have recently attracted *** paper first briefly introduces traditional technologies related to the conversion of biomass to electricity and hydrogen,and then reviews the new developments in flow biomass fuel cells(FBFCs)and biomass electrolysis for hydrogen production(BEHP)in *** challenges in these areas are discussed.

关键词： Biomass Flow cell Fuel cell Hydrogen production Electrolysis

Aptamer-Based Fluorescent Assay for Sensitive Detection of Cardiac Troponin Ⅰ

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Journal of Beijing Institute of Technology 2020年第1期29卷 45-51页

作者： Yu Li Yuanzhan Yang Xuefei Lu yulin deng School of Life Science Beijing Institute of TechnologyBeijing 100081China

Acute myocardial infarction(AMI)is a major health problem leading to high rates of mortality and *** cardiac troponin I(cTnI)has shown high sensitivity and specificity towards AMI detection,and has been regarded as"gold standard".An ultrasensitive method to detectcTnI with low concentration in human fluid is *** this paper,we developed an aptamer-based assay coupled with rolling circle amplification(RCA)and molecular beacon probe for sensitive detection *** this strategy,aptamer acts as a bridge to communicate between oligonucleotides *** reaction produces a single-stranded tandem repeated copy of the circular template,which are recognized by fluorescence molecular beacon *** this strategy,highly sensitive and specific detection of cTnI was realized with the lowest detectable concentration of 7.24 pg/m *** developed aptamer-RCA assay can be a promising tool in clinical samples *** assay can also analyze other disease-related biomarkers by replacing the aptamer.

关键词： aptamer cardiac troponin I(cTnI) molecular beacon rolling circle amplification sensitive detection

Investigation on P-Glycoprotein Function and Its Interacting Proteins under Simulated Microgravity

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Space(Science & Technology) 2021年第1期2021卷 195-207页

作者： Yujuan Li Lili Huang Javed Iqbal yulin deng School of Life Sciences Beijing Institute of TechnologyBeijing 100081China Department of Biology Government College MankeraUniversity of SargodhaSargodhaPakistan

P-glycoprotein(P-gp)could maintain stability of the nerve system by effluxing toxins out of the blood-brain *** it plays a very important role in drug brain distribution during space travel is not yet *** present study was aimed at investigating P-gp function,expression,and its interacting proteins in a rat brain under simulated microgravity(SMG)by comparative proteomics *** were tail-suspended to induce short-(7-day)and long-term(21-day)microgravity.P-gp function was assessed by measuring the P-gp ATPase activity and the brain-to-plasma concentration ratio of rhodamine 123.P-gp expression was evaluated by Western blot.21d-SMG significantly enhanced P-gp efflux activity and expression in ***-free proteomics strategy identified 26 common differentially expressed proteins(DEPs)interacting with P-gp in 7d-and 21d-SMG *** of the DEPs mainly regulated ATP hydrolysis coupled transmembrane transport and so *** analysis showed that P-gp might potentially interact with heat shock proteins,sodium/potassium ATP enzyme,ATP synthase,microtubule-associated proteins,and vesicle fusion *** present study firstly reported P-gp function,expression,and its potentially interacting proteins exposed to simulated *** findings might be helpful not only for further study on nerve system stability but also for the safe and effective use of P-gp substrate drugs during space travel.

关键词： drugs travel maintain

Comparative Proteomics Based on 18O Labeling to Evaluate the Hepatotoxicity of Tris（2,3-dibromopropyl） Isocyanuratein Rats

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Journal of Beijing Institute of Technology 2019年第3期28卷 659-666页

作者： Yu Li Xuefei Lü Yuanzhan Yang yulin deng School of Life Science Beijing Institute of Technology

Detecting protein expression levels in organisms exposed to environmental pollutants can help us understand the action mechanism of toxicity. In the present study, adult female Sprague-Dawley (SD) rats received a consecutive intraperitoneal injection of tris(2,3-dibromopropyl) isocyanurate (TBC) for seven days, and the ^16O/^18O labeling comparative proteomic approach was used to study the change of liver proteome in these rats. Thirty six differential proteins were identified between the control group and the high-dose-exposed group (8 mg/kg), of which, twenty eight proteins were down-regulated and eight proteins were up-regulated. Bioinformatics analysis revealed that most of the differential proteins were related with the metabolic and cellular processes. In addition, the contents of malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) in liver were measured to evaluate the oxidative stress status induced by TBC exposure. Together, these findings showed that TBC might be toxic to liver by disrupting metabolic process and apoptosis. The results might provide a better insight into the mechanism of toxicity induced by TBC.

关键词： comparative proteomic hepatotoxicity ^18O labeling oxidative stress tris(2,3-dibromopropyl) isocyanurate (TBC)

Microfluidics for cell-cell interactions： A review

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Frontiers of Chemical Science and Engineering 2016年第1期10卷 90-98页

作者： Rui Li Xuefei Lv Xingjian Zhang Omer Saeed yulin deng School of Life Science Beijing Institute of Technology Beijing 100081 China

Micro fluidic chip has been applied in various biological fields owing to its low-consumption of reagents, high throughput, fuidic controllability and integrity. The well-designed microscale intermediary is also ideal for the study of cell biology. Particularly, microfluidic chip is helpful for better understanding cell-cell interactions. A general survey of recent publications would help to generalize the designs of the co-culture chips with different features. With ingenious and combinational utilization, the chips facilitate the implementation of some special coculture models that are highly concerned in a different spatial and temporal way.

关键词： microfluidic chip co-culture cell-cell interactions review

Determination of the total concentration of casein and ?-lactoglobulin by indirect competitive ELISA in milk and dairy products

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Chinese Science Bulletin 2014年第27期59卷 3385-3389页

作者： Xuefei Lv Xuena Hu Yan Man yulin deng School of Life Science Beijing Institute of Technology

The main purpose of this work was to develop an assay as a means of quality control in milk and dairy products. As specific and high abundant proteins in milk and diary products, casein and b-lactoglobulin were chosen as the targets, and an indirect competitive enzyme-linked immunosorbent assay(ELISA) was developed for determination of the total concentration of casein and b-lactoglobulin. The detection limit of the assay was 79.07 ng/mL,and a working range of the calibration curve was from140.6 to 36,000 ng/mL. The intra-assay and inter-assay coefficients of variations were 4.7 % and 6.5 %, respectively. The recoveries ranged from 94.1 % to 106.6 %. The developed ELISA assay was used to detect the total concentration of casein and b-lactoglobulin in liquid milk and milk powder. The results indicated that the developed assay was very specific, and the addition of nonprotein nitrogen(such as melamine, cyanuric acid, and urea) and vegetable protein did not affect the detection. The good performance of the assay makes it suitable for use in the quality control of milk and dairy products.

关键词：酶联免疫吸附测定乳制品球蛋白酪蛋白总浓度牛奶竞争间接

Space: Science & Technology-Promoting Academic Exchange and Exploring the Frontiers of Space

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Space(Science & Technology) 2021年第1期2021卷 324-325页

作者： Peijian Ye yulin deng Michel Blanc China Academy of Space Technology China Beijing Institute of Technology China Institut de Recherche en Astrophysique et Planétologie (CNRS-University Toulouse III Paul Sabatier-CNES)France

People on Earth,the cradle and common home of humankind,pursue a shared dream to explore the vast universe and understand the relationship between humankind and *** ancient times to the present,human beings have always taken great interest in exploring and understanding Earth itself and its position in the *** sciences like astronomy,cosmology,space science,and planetary science,as well as material science,fluid science,and life science,were all born in response to this“call from the universe.”

关键词： Earth universe astronomy

Comprehensive Evaluation on Agricultural Environment in Western China

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Journal of Mountain Science 2005年第3期2卷 244-254页

作者： TU Jianjun deng yulin School of Resources and Environmental Science Southwest University Beibei 400715 Chongqing China Sichuan Agricultural University Ya＇an 625014 Sichuan China

Taking provinces as study cases, 15 comprehensive factors, involving natural geography, agricultural production, economic and social information, were formulated to evaluate the agricultural environment in western China by factor analysis method. The results showed that comprehensive scores were distinct among different cases. The score of Sichuan was the highest, that of Chongqing second and that of Guangxi the third. Qinghai got the lowest score, ranking the twelfth. The general environment quality was better in moist southern regions than that in frigid northern regions in the provinces of western China. Therefore, the appropriate eco-environmental construction measures at provincial level should be proposed to increase vegetation coverage, control water and soil loss, and prevent desertification and wind erosion. To develop eco-agriculture is an inevitable selection for environment construction and improvement in western China.

关键词： Western China agricultural environment quantitative evaluation factor analysis strategy

Tris-(2,3-dibromopropyl)Isocyanurate Induced Oxidative Stress in the Human Neuronal Cell Lines and in Rat Brains

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Journal of Beijing Institute of Technology 2018年第3期27卷 468-476页

作者： Wei Feng Yu Li Jing Zhang Xuefei Lyu yulin deng School of Life Science Beijing Institute of Technology

Tris-(2,3-dibromopropyl)isocyanurate(TBC)is a heterocyclic hexabrominated additive flame retardant,which is bio-accumulative,and can cause reproductive,endocrine disrupting,and neurotoxic *** present study was aimed at further evaluating the oxidative stress induced by TBC in the human neuronal cells and in rat *** results demonstrated that TBC caused apoptosis of U251 and SH-SY5 Y cells in a dose-and time-dependent manner and that U251 cells were more sensitive to TBC than that of SH-SY5 Y ***,1μg/mL of TBC can significantly induce the production of MDA in U251 cells,indicating that oxidative stress occurred after TBC short-term exposure(24 h).Similarly,in vivo administration of 0.5 mg/kg of TBC in rats for 7 days led to low growth rates and a significant increase of reactive oxygen species(ROS)and nitrogen species(RNS)in the ***,enzymes related to antioxidation,total superoxide dismutase(T-SOD)and reduced glutathione(GSH),were not affected *** might indicate that 7-day exposure was not long enough to weaken antioxidant defence in the ***,the results indicated that oxidative stress was induced by short-time TBC exposure.

关键词： tris-(2,3-dibromopropyl)isocyanurate(TBC) oxidative stress human neuronal cell lines rat brain