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Hemoglobin S Polymerization Effect on Water Self-Diffusion Coefficient

Advances in Biological Chemistry 2014年第6期4卷 388-394页

作者： Manuel A. Lores Guevara Juan C. García-Naranjo yulianela mengana Jorge Pereira Magnetic Resonance Lab Biophysics and Medical Physics Centre Santiago de Cuba Cuba

The transversal relaxation time, the effective transversal relaxation time and the water self-diffusion coefficient are evaluated during hemoglobin S polymerization. One homogeneous permanent magnet and one inhomogeneous and portable unilateral magnet with a very strong and constant static magnetic field gradient were utilized. The Carr-Purcell-Meiboom-Gill method was used before and after placing the studied samples 24 hours at 36°C to guarantee the polymerization. The transversal relaxation shows two exponents after polymerization supporting the concept of partially polymerized hemoglobin. The effective transversal relaxation time decreases around 40%, which can be explained by the increase of water self-diffusion coefficient 1.8 times as a main value. This result can be explained considering the effects of the agglutination process on the obstruction and hydration effects in a partially polymerized solution.

关键词： Sickle Cell Disease Water Self-Diffusion Coefficient T2 NMR

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Water Efflux in Red Blood Cells of Sickle Cell Patients under Spontaneous Deoxygenation

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Journal of Biosciences and Medicines 2016年第12期4卷 152-161页

作者： Manuel Arsenio Lores Guevara yulianela mengana Torres Juan Carlos García Naranjo Andrés Ramírez Aguilera Lidia Clara Suárez Beyrio María Altagracia Marichal Felue Teresa Simón Brada Jan Philippé Centro de Biofí sica Mé dica Santiago de Cuba Cuba Hospital General “Juan Bruno Zayas Alfonso” Santiago de Cuba Cuba Department of Clinical Chemistry Immunology and Microbiology Ghent University Ghent Belgium

The water transport through Red Blood Cells (RBC) membrane has been previously studied in Sickle Cell Disease (SCD) using oxygenated RBC or under complete deoxygenation. In this work, the water efflux in RBC of sickle cell patients was studied under spontaneous deoxygenation conditions. With that purpose, a magnetic resonance method was used to evaluate the water exchange time (τe) and the permeability through the erythrocyte membrane (P) measuring the spin-spin relaxation time (T2) in doped and non-doped RBC. Carr-Purcell-Meiboon-Gill (CPMG) pulse sequence was used to measure T2 in a magnetic resonance console coupled to one homogeneous magnet system (0.095 T). An increase of the water transport in RBC from sickle cell patients was observed and characterized with a τe value of 15.2 ± 0.8 ms. The abnormal activation of the Psickle, Gardos, and potassium chloride cotransporter channels starting from deoxygenation, as well as, the possible appearance of new pores due to the increase of the hemoglobin-membrane interaction, are suggested to explain this abnormal transport phenotype. The change of the water volume to surface ratio (V/S) in the sickle cells is also suggested to be considered in P calculation under deoxygenation. The results obtained in this work increase the fundamental knowledge about molecular mechanism involved in SCD and could be useful in the development of new methods for diagnostic and treatment evaluation.

关键词： Red Blood Cells Water Permeability Nuclear Magnetic Relaxation Sickle Cell Disease

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