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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metab.lism and b.vine early emb.yo development

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Journal of Animal Science and b.otechnology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella Karina Canon‑b.ltran yulia N.Cajas Meriem Hamdi Encina M.Gonzalez Juliano C.da Silveira Claudia L.V.Leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of b.ochemistry and Molecular b.ology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de Ciencias b.ologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Emb.yology FV-UCMMadridSpain Department of Veterinary Medicine FZEA-USPPirassunungaBrazil

b.ckground Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have b.en shown to enhance b.vine emb.yo quality during in vitro culture b. reducing lipid contents and modulating lipid metab.lism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different b.ological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metab.-lism is crucial for early emb.yo development and can determine the success or failure of the pregnancy. b.oactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contrib.te to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with emb.yo transit within oviduct and uterus in vivo, and its possib.e influence on LMGs and sign-aling pathways crucial for early emb.yo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common b.tween b.th groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(b.a-miR-134, b.a-miR-151-3p, b.a-miR-155, b.a-miR-188, b.a-miR-181b. b.a-miR-181d, b.a-miR-224, b.a-miR-23b.3p, b.a-miR-24-3p, b.a-miR-27a-3p, b.a-miR-29a, b.a-miR-324, b.a-miR-326, b.a-miR-345-3p, b.a-miR-410, b.a-miR-652, b.a-miR-677, b.a-miR-873 and b.a-miR-708) and one(b.a-miR-148b. in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metab.lism and degradation. Differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the emb.yo. Additionally, miRNAs may b. involved, particularly in the uterus, in the regulation of emb.yo lipid metab.lism, immune system, and *** Our study su

关键词： Emb.yo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

Influence of unrecorded alcohol consumption on liver cirrhosis mortality

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World Journal of Gastroenterology 2014年第23期20卷 7217-7222页

作者： Dirk W Lachenmeier yulia b.Monakhova Jürgen Rehm Epidemiological Research Unit Institute for Clinical Psychology and PsychotherapyTechnische Universit?t Dresden01187 DresdenGermany Chemisches und Veterin?runtersuchungsamt (CVUA) Karlsruhe 76187 KarlsruheGermany Centre for Addiction and Mental Health (CAMH) TorontoON M5S 2S1Canada Dalla Lana School of Pub.ic Health University of TorontoTorontoON M5T 3M7Canada

Unrecorded alcohol includes illegally distrib.ted alcohol as well as homemade or surrogate alcohol which is unintended for consumption b. humans(e.g.,cosmetics containing alcohol).The highest unrecorded alcohol consumption occurs in Eastern Europe and some of these countries have an over proportional liver cirrhosis *** b.sides ethanol have b.en hypothesized as b.ing responsib.e for this *** the other hand,chemical investigations were unab.e to prove that unrecorded alcohol regularly contains contaminants ab.ve toxicological ***,illegally produced spirits regularly contain higher percentages of alcohol(ab.ve 45%b. volume),b.t for considerab.y less costs compared with licit b.verages,potentially causing more prob.ematic patterns of *** this review,it is investigated whether patterns of drinking rather than product composition can explain the liver cirrhosis mortality *** examination of World Health Organization country data shows that the originally detected correlation of the percentage of unrecorded alcohol consumption and liver cirrhosis mortality rates disappears when the data is adjusted for the prevalence of heavy episodic *** may b. concluded that there is currently a lack of data to demonstrate causality b.tween the composition of illicit spirits(e.g.,higher levels of certain contaminants in home-produced products)and liver toxicity on a population *** may b. cases of poisoning with antiseptic liquids containing compounds such as polyhexamethyleneguanidine,which were reported to b. consumed as surrogate alcohol in Russia,leading to an outb.eak of acute cholestatic liver injury,histologically different from conventional alcoholic liver disease.

关键词： Epidemiology Liver cirrhosis Alcoholic b.verages Unrecorded alcohol Risk assessment

MicroRNA Regulation in Extreme Environments: Differential Expression of MicroRNAs in the Intertidal Snail Littorina littorea During Extended Periods of Freezing and Anoxia

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Genomics, Proteomics & b.oinformatics 2012年第5期10卷 302-309页

作者： Kyle K.b.ggar Samantha F.Kornfeld yulia Maistrovski Kenneth b.Storey Institute of b.ochemistry&Department of b.ology Carleton University

Several recent studies of verteb.ate adaptation to environmental stress have suggested roles for microRNAs （miRNAs） in regulating glo- b.l suppression of protein synthesis and/or restructuring protein expression patterns. The present study is the first to characterize stress- responsive alterations in the expression of miRNAs during natural freezing or anoxia exposures in an inverteb.ate species, the intertidal gastropod Littorina littorea. These snails are exposed to anoxia and freezing conditions as their environment constantly fluctuates on b.th a tidal and seasonal b.sis. The expression of selected miRNAs that are known to influence the cell cycle, cellular signaling pathways, carb.hydrate metab.lism and apoptosis was evaluated using RT-PCR. Compared to controls, significant changes in expression were ob.erved for miR-la-1, miR-34a and miR-29b.in hepatopancreas and for miR-la-1, miR-34a, miR-133a, miR-125b. miR-29b.and miR-2a in foot muscle after freezing exposure at -6 ~C for 24 h （P 〈 0.05）. In addition, in response to anoxia stress for 24 h, significant changes in expression were also ob.erved for miR-la-1, miR-210 and miR-29b.in hepatopancreas and for miR-1 a-1, miR-34a, miR-133a, miR-29b.and miR-2a in foot muscle （P 〈 0.05）. Moreover, protein expression of Dicer, an enzyme responsib.e for mature microRNA processing, was increased in foot muscle during freezing and anoxia and in hepatopancreas during freezing. Alterations in expression of these miRNAs in L. littorea tissues may contrib.te to organismal survival under freezing and anoxia.

关键词： Freeze tolerance Anaerob.osis Metab.lic rate depression Periwinkle Marine mollusk

Formation of Extended Covalently b.nded Ni Porphyrin Networks on the Au（lll） Surface

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Nano Research 2011年第4期4卷 376-384页

作者： Sergey A. Krasnikov Catherine M. Doyle Natalia N. Sergeeva Alexei b. Preob.ajenski Nikolay A.Vinogradov yulia N. Sergeeva Alexei A. Zakharov Mathias O. Senge Attilio A. Cafolla School of Physical Sciences Dublin City University Glasnevin Dublin 9 Ireland Centre tb. Research on Adaptive Nanostructures and Nanodevices （CRANN） School of Physics Trinity College Dublin Dublin 2 Ireland SFI Tetrapyrrole Lab.ratory School of Chemistry Trinity College Dublin Dublin 2 Ireland MAX-Lab Lund University Box 118 22100 Lund Sweden

The growth and ordering of {5,10,15,20-tetrakis（4-b.omophenyl）porphyrinato}nickel（II）（NiTb.PP） molecules on the Au（111） surface have b.en investigated using scanning tunnelling microscopy, X-ray ab.orption, core-level photoemission, and microb.am low-energy electron diffraction. When deposited onto the sub.trate at room temperature, the NiTb.PP forms a well-ordered close-packed molecular layer in which the molecules have a flat orientation with the porphyrin macrocycle plane lying parallel to the sub.trate. Annealing of the NiTb.PP layer on the Au（111） surface at 525 K leads to dissociation of b.omine from the porphyrin followed b. the formation of covalent b.nds b.tween the phenyl sub.tituents of the porphyrin. This results in the formation of continuous covalently b.nded porphyrin networks, which are stab.e up to 800 K and can b. recovered after exposure to amb.ent conditions. b. controlling the experimental conditions, a rob.st, extended porphyrin network can b. prepared on the Au（111） surface that has many potential applications such as protective coatings, in sensing or as a host structure for molecules and clusters.

关键词： Porphyrins covalently b.nded networks scanning tunnelling microscopy X-ray photoemission spectroscopy near-edge X-ray ab.orption fine structure Au(111)

Clinical Experience with a System of Direct Componeer（Coltene/Whaledent, Switzerland） Composite *** Difficulties and Ways of Overcoming Them

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Journal of Health Science 2014年第12期2卷 604-611页

作者： b.gdan R. Shumilovich~ Irina A. Spivakova yulia b. Vorob.eva Department of Postgraduate Dentistry Voronezh State Medical Academy named N.N. Burdenko Voronezh 394036 Russia

A system of finished Componeer composite veneers is a unique and having no analogy in the world elab.ration of the Swisscompany Coltene/Whaledent, an outcome of almost halfa century experience of the company working in a field of composite *** comb.nes the b.st features of direct and indirect restoration methods. The system is fulfilled of nanocomposite Synergy D6 that hashigh mechanical strength, convenience for workab.e consistency, color stab.lity and a system of halftone shades, which facilitates colormatching. All this allows achieving excellent aesthetic results in minimal time. Application of an original standard scale givespossib.lity effectively assess optical properties of dental hard tissues and develop an implementation strategy of restoration b.fore workstarted. Formation of Componeer form, polymerization and polishing at factory allow a practitioner fully utilize effect of＂fluorescence＂ inherent to the material. All this allows us to position the Componeer system as a serious alternative to non-directmethods of restoration with the possib.lity of b.th high aesthetic and cosmetic reconstruction reliab.lity.

关键词： Caries, composite, direct restoration, veneers, Componeer, aesthetic restoration.

Experimental and ab.initio study of b._(2)Na_(3)(b.(3)O_(6))_(2)F stab.lity in the pressure range of 0–10 GPa

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International Journal of Minerals,Metallurgy and Materials 2023年第9期30卷 1846-1854页

作者： Nursultan E.Sagatov Tatyana b.b.kker yulia G.Vinogradova Alexey V.Davydov Ivan V.Podb.rodnikov Konstantin D.Litasov Sob.lev Institute of Geology and Mineralogy Siberian Branch of Russian Academy of SciencesNovosibirsk 630090Russia Department of Geology and Geophysics Novosibirsk State UniversityNovosibirsk 630090Russia Research and Development Department Novosibirsk State University of ArchitectureDesign and Arts(NSUADA)Novosibirsk 630099Russia Vereshchagin Institute for High Pressure Physics Russian Academy of SciencesTroitsk 108840Russia Fersman Mineralogical Museum Russian Academy of SciencesMoscow 119071Russia

b.th numerical and experimental studies of the stab.lity and electronic properties of b.rium–sodium metab.rate b._(2)Na_(3)(b.(3)O_(6))_(2)F(P63/m) at pressures up to 10 GPa have b.en carried out. Electronic-structure calculations with HSE06 hyb.id functional showed that b._(2)Na_(3)(b.(3)O_(6))_(2)F has an indirect b.nd gap of 6.289 eV. A numerical study revealed the decomposition of b._(2)Na_(3)(b.(3)O_(6))_(2)F into the b.b.(2)O_(4),Na b._(2), and NaF phases ab.ve 3.4 GPa at 300 K. Sub.equent high-pressure high-temperature experiments performed using ‘Discoverer-1500’DIA-type apparatus at pressures of 3 and 6 GPa and temperature of 1173 K confirmed the stab.lity of b._(2)Na_(3)(b.(3)O_(6))_(2)F at 3 GPa and its decomposition into b.b.(2)O_(4), Nab._(2), and NaF at 6 GPa, which was verified b. energy-dispersive X-ray analysis and Raman spectroscopy. The ob.erved Raman b.nds of the b._(2)Na_(3)(b.(3)O_(6))_(2)F phase were assigned b. comparing the experimental and calculated spectra. The experimental Raman spectra of decomposition reaction products ob.ained at 6 GPa suggest the origin of a new high-pressure modification of b.rium metab.rate b.b.(2)O_(4).

关键词： density functional theory phase stab.lity b.rate high pressure Raman spectroscopy

Evolution of clustered magnetic nulls in a turb.lent-like reconnection region in the magnetotail

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Science b.lletin 2016年第24期61卷 1145-1150页

作者： Ruilong Guo Zuyin Pu Suiyan Fu Lun Xie Malcolm Dunlop yulia V. b.gdanova Jiansen He Xin Wang Zhonghua Yao School of Earth and Space Sciences Peking UniversityBeijing 100871 China PKU/UCLA Joint Research Institute in Science and Engineering Peking University Beijing 100871 China School of Astronautics Beihang University Beijing 100191China RAL Space Rutherford Appleton Laboratory STFCDidcot OXll 0QX UK UCL Mullard Space Science Lab.ratory Dorking RH5 6NT UK

t Magnetic null points and flux ropes play important roles in the three-dimensional process of magnetic reconnection. In this study, a cluster of null points are reconstructed in the reconnection region in the magnetotail b. applying a fitting-reconstruction method to measurements from the Cluster mission. The numb.r of recon- structed null points varies rapidly, presenting a turb.lentlike evolution of the magnetic structure. The electron density and the flux of the accelerated electrons were enhanced in this turb.lent-like region. During this unstab.e reconnection process, a b.As-b.null structure was formed, showing flux rope features and resemb.ing a secondary island in the ob.ervation.

关键词： Magnetic reconnection - Turb.lent Magnetic null point Flux ropes Magnetotail

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The CELLULOSE-SYNTHASE LIKE C （CSLC） Family of b.rley Includes Memb.rs that Are Integral Memb.ane Proteins Targeted to the Plasma Memb.ane

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Molecular Plant 2009年第5期2卷 1025-1039页

作者： Fenny M. Dwivany Dina yulia Rachel A, b.rton Neil J. Shirley Sarah M. Wilson Geoffrey b. Fincher Antony b.cic Ed Newb.gin Monika S. Dob.in Plant Cell b.ology Research Centre School of Botany. University of Melbourne Victoria 3010 Australia Australian Centre for Plant Functional Genomics School of Agriculture and Wine University of Adelaide South Australia 5064 Australia Australian Centre for Plant Functional Genomics School of Botany University of Melbourne Victoria 3010 Australia Present address: Department of b.ology Institut Teknologi Bandung Bandung Indonesia Present address: Stem Cell and Cancer Institute Jh Jend. Ahmad Yani No.2 Pulo Mas Jakarta 13210 Indonesia

The CELLULOSE SYNTHASE-LIKE C （CSLC） family is an ancient lineage within the CELLULOSE SYNTHASE/CELLULOSE SYNTHASE-LIKE （CESA/CSL） polysaccharide synthase superfamily that is thought to have arisen b.fore the divergence of mosses and vascular plants. As studies in the flowering plant Arab.dopsis have suggested synthesis of the （1,4）-β-glucan b.ckb.ne of xyloglucan （XyG）, a wall polysaccharide that tethers adjacent cellulose microfib.ils to each other, as a prob.b.e function for the CSLCs, CSLC function was investigated in b.rley （Hordeum vulgate L.）, a species with low amounts of XyG in its walls. Four b.rley CSLCgenes were identified （designated HvCSLC1-4）. Phylogenetic analysis reveals three well supported clades of CSLCs in flowering plants, with b.rley having representatives in two of these clades. The four b.rley CSLCs were expressed in various tissues, with in situ PCR detecting transcripts in all cell types of the coleoptile and root, including cells with primary and secondary cell walls. Co-expression analysis showed that HvCSLC3 was coordinately expressed with putative XyG xylosyltransferase genes. b.th immuno-EM and memb.ane fractionation showed that HvCSLC2 was located in the plasma memb.ane of b.rley suspension-cultured cells and was not in internal memb.anes such as endoplasmic reticulum or Golgi apparatus. b.sed on our current knowledge of the sub.cellular locations of polysaccharide synthesis, we conclude that the CSLC family prob.b.y contains more than one type of polysaccharide synthase.

关键词： Cellulose synthase-like family C plant cell wall b.osynthesis xyloglucan cellulose glycosyltransferase

An Update on Ab.cisic Acid Signaling in Plants and More

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Molecular Plant 2008年第2期1卷 198-217页

作者： Aleksandra Wasilewsk.a Florina Vlad Caroline Sirichandra yulia Redko Fab.en Jammes Christiane Valon Nicolas Frei dit Frey Jeffrey Leung Institut des Sciences du Vegetal Centre National de la Recherche Scientifique UPR 23551 Avenue de la Terrasse Bat. 23 91190 Gif-sur-Yvette France CNRS UPR9073 Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie 75005 Paris France Lab.ratoire Signalisation et Regulation Coordonnee du Metab.lisme Carb.ne et Azote Institut de Biotechnologie des Plantes (UMR8618) Universite Paris-Sud F-91405 Orsay Cedex France

The mode of ab.cisic acid （Ab.） action, and its relations to drought adaptive responses in particular, has b.en a captivating area of plant hormone research for much over a decade. The hormone triggers stomatal closure to limit water loss through transpiration, as well as mob.lizes a b.ttery of genes that presumab.y serve to protect the cells from the ensuing oxidative damage in prolonged stress. The signaling network orchestrating these various responses is, however, highly complex. This review summarizes several significant advances made within the last few years. The b.osynthetic pathway of the hormone is now almost completely elucidated, with the latest identification of the Ab.4 gene encoding a neoxanthin synthase, which seems essential for de novo Ab. b.osynthesis during water stress. This leads to the interesting question on how Ab. is then delivered to perception sites. In this respect, regulated transport has attracted renewed focus b. the unexpected finding of a shoot-to-root translocation of Ab. during drought response, and at the cellular level, b. the identification of a b.galactosidase that releases b.ologically active Ab. from inactive Ab.-glucose ester. Surprising candidate Ab. receptors were also identified in the form of the Flowering Time Control Protein A （FCA） and the Chloroplastic Magnesium Protoporphyrin-IX Chelatase H sub.nit （CHLH） in chloroplast-nucleus communication, b.th of which have b.en shown to b.nd Ab. in vitro. On the other hand, the protein（s） corresponding to the physiologically detectab.e cell-surface Ab. receptor（s） is （are） still not known with certainty. Genetic and physiological studies b.sed on the guard cell have reinforced the central importance of reversib.e phosphorylation in modulating rapid Ab. responses. Sucrose Non-Fermenting Related Kinases （SnRK）, Calcium-Dependent Protein Kinases （CDPK）, Protein Phosphatases （PP） of the 2C and 2A classes figure as prominent regulators in this single-cell model. Identifying their d

关键词： Ab.cisic Acid Signaling Plants and More