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Antithrombotic Effects of Different Strains of Lactic Acid Bacteria

Health 2014年第18期6卷 2433-2443页

作者： Hiromasa Ooe Masaru Kato Kanae Hyodo Keiko Nakashima Hiroshi Ashigai Kyoko Kato Toshinori Sasaki yuko fukushima John C. Giddings Junichiro Yamamoto Research Laboratories for Health Science and Food Technologies Kirin Company Limited Yokohama Japan Research Laboratories for Brewing Technologies Kirin Company Limited Yokohama Japan Laboratory of Physiology Faculty of Nutrition Kobe Gakuin University Kobe Japan Central Laboratories for Key Technologies Kirin Company Limited Yokohama Japan Formerly Department of Haematology School of Medicine Cardiff University UK

Objective: The aim of the present study was to assess the antithrombotic properties of different strains of orally available antithrombotic lactic acid bacteria (LAB). Research Methods & Procedures: Antithrombotic activity, antiplatelet reactivity and/or thrombolytic activity, were measured in seven strains of LAB and LAB cell-free-extracts (LAB filtrates) using the shear-induced platelet reactivity/thrombolytic activity, The Global Thrombosis Test (GTT), with non-anticoagulated rat blood in vitro. Subsequently, the most potent antithrombotic strains identified in vitro were assessed in vivo after oral administration in mouse carotid arteries using a helium-neon laser-induced thrombosis model. Result: Five strains out of seven LAB (Lactobacillus paracasei KW 3100, Lactobacillus fermentum NBRC 3961, Lactobacillus pentosus JCM 8333, Leuconostoc oeni Elios 1, Pediococcus pentosaceus NK-2) promoted significant endogenous thrombolysis in vitro. In addition, one strain (Lactobacillus fermentum NBRC 3961) significantly inhibited shear-induced platelet re-activity. Three antithrombotic strains, Lactobacillus fermentum NBRC 3961, Leuconostoc oeni Elios 1, and Lactobacillus pentosus JCM 8333, were further assessed in vivo. The results demonstrated that filtrates, and the cells per se of these LAB, modulated antiplatelet activity and/or thrombolytic activity, and that the antithrombotic mechanisms were mainly influenced by protein content (60% - 70% of dry matter). Conclusion: The findings suggested that some strains of lactic acid bacteria could usefully provide the basis for the production of oral antithrombotic probiotics.

关键词： Antithrombotic Lactic Acid Bacteria Shear-Induced Thrombosis Global Thrombosis Test (GTT) Laser-Induced Thrombosis Endogenous Thrombolysis

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Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism

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Molecular Plant 2010年第1期3卷 125-142页

作者： Doris Albinsky Miyako Kusano Mieko Higuchi Naomi Hayashi Makoto Kobayashi Atsushi fukushima Masaki Mori Takanari Ichikawa Keiko Matsui Hirofumi Kuroda Yoko Horii yuko Tsumoto Hitoshi Sakakibara Hirohiko Hirochika Minami Matsui Kazuki Saito RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Inage-ku Chiba 263-8522 Japan

Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length （FL） cDNAs （rice FOX Arabidopsis lines） using a gas chromatography-time-of-flight mass spectrometry （GC-TOF/MS）-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candi- date lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Ara- bidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries （LOB） Domain （LBD）/ Asymmetric Leaves2-1ike （ASL） gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.

关键词： Metabolite profiling GC-TOF/MS rice FOX Arabidopsis lines transcript profiling LBD37/ASL39 nitrogenmetabolism.

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Comparison between surgical outcomes of colorectal cancer in younger and elderly patients

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World Journal of Gastroenterology 2011年第12期17卷 1642-1648页

作者： Longxue Jin Norio Inoue Naoki Sato Susumu Matsumoto Hitoshi Kanno yuko Hashimoto Kazuhiro Tasaki Kinya Sato Shun Sato Katsutoshi Kaneko Center for Digestive Diseases Northern Fukushima Medical Center Fukushima 960-0502 Japan Department of Pathology Fukushima Medical University Hospital Fukushima 960-1295 Japan Division of Anesthesiology Northern Fukushima Medical Center Fukushima 960-0502 Japan

AIM:To compare the outcome of surgical treatment of colorectal adenocarcinoma in elderly and younger ***:The outcomes of 122 patients with colorectal adenocarcinoma who underwent surgical treatment between January 2004 and June 2009 were *** clinicopathological and blood biochemistry data of the younger group(<75 years) and the elderly group (≥75 years) were ***:There were no significant differences between the two groups in operation time,intraoperative blood loss,hospital stay,time to resumption of oral intake,or *** elderly group had a significantly higher rate of hypertension and cardiovascular *** perioperative serum total protein and albumin levels were significantly lower in the elderly than in the younger *** serum carcinoembryonic antigen level was lower in the elderly than in the younger group,and there was a significant decreasing trend after the operation in the elderly ***:The short-term outcomes of surgical treatment in elderly patients with colorectal adenocarcinoma were *** treatment in elderly patients was considered a selectively effective approach.

关键词： Colorectal tumor Elderly patient Morbidity Carcinoembryonic antigen C-reactive protein

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Contrast uptake in primary hepatic angiosarcoma on gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid-enhanced magnetic resonance imaging in the hepatobiliary phase

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World Journal of Hepatology 2018年第1期10卷 166-171页

作者： Manabu Hayashi Satoshi Kawana Hirofumi Sekino Kazumichi Abe Naoki Matsuoka Masahito Kashiwagi Ken Okai Yukiko Kanno Atsushi Takahashi Hiroshi Ito yuko Hashimoto Hiromasa Ohira Department of Gastroenterology Fukushima Medical University School of Medicine Department of Diagnostic Pathology Fukushima Medical University School of Medicine Department of Radiology Fukushima Medical University School of Medicine

Primary hepatic angiosarcoma is the most common malignant mesenchymal tumor of the liver. It has a poor prognosis and various appearances on magnetic resonance(MR) images. We report a case of hepatic angiosarcoma with a characteristic appearance on gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid(Gd-EOB-DTPA)-enhanced MR imaging in the hepatobiliary phase. A 72-year-old man was admitted with a complaint of abdominal pain. Gd-EOBDTPA-enhanced MR imaging revealed a liver tumor that showed slight hyperintensity in the hepatobiliary phase. These findings suggested Gd-EOB-DTPA uptake in the tumor. An autopsy revealed the solid proliferation and sinusoidal spreading of hepatic angiosarcoma cells. Immunohistochemistry indicated that the tumor was negative for OATP1B3. Gd-EOB-DTPA uptake in the liver tumor in the hepatobiliary phase suggested sinusoidal tumor invasion with residual normal hepatocytes.

关键词： Hepatic angiosarcoma Gadoliniumethoxybenzyl-diethylenetriamine pentaacetic acid Cirrhosis Hepatocellular carcinoma

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Push vs pull method for endoscopic ultrasound-guided fine needle aspiration of pancreatic head lesions: Propensity score matching analysis

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World Journal of Gastroenterology 2018年第27期24卷 3006-3012页

作者： Mitsuru Sugimoto Tadayuki Takagi Rei Suzuki Naoki Konno Hiroyuki Asama Yuki Sato Hiroki Irie Ko Watanabe Jun Nakamura Hitomi Kikuchi Yuichi Waragai Mika Takasumi Minami Hashimoto yuko Hashimoto Takuto Hikichi Hiromasa Ohira Department of Gastroenterology Fukushima Medical UniversitySchool of Medicine Department of Endoscopy Fukushima Medical University Hospital Department of Diagnostic Pathology Fukushima Medical University

AIM To evaluate the efficacy of endoscopic ultrasoundguided fine needle aspiration(EUS-FNA) of pancreatic head cancer when pushing(push method) or pulling the echoendoscope(pull method).METHODS Overall, 566 pancreatic cancer patients had their first EUS-FNA between February 2001 and December 2017. Among them, 201 who underwent EUS-FNA for pancreatic head lesions were included in this study. EUS-FNA was performed by the push method in 85 patients, the pull method in 101 patients and both the push and pull methods in 15 patients. After propensity score matching(age, sex, tumor diameter, and FNA needle), 85 patients each were stratified into the push and pull groups. Patient characteristics and EUSFNA-related factors were compared between the two *** Patient characteristics were not significantly different between the two groups. The distance to lesion was significantly longer in the push group than in the pull group(13.9 ± 4.9 mm vs 7.0 ± 4.9 mm, P < 0.01). The push method was a significant factor influencing the distance to lesion(≥ median 10 mm)(P < 0.01). Additionally, tumor diameter ≥ 25 mm(OR = 1.91, 95%CI: 1.02-3.58, P = 0.043) and the push method(OR = 1.91, 95%CI: 1.03-3.55, P = 0.04) were significant factors contributing to the histological diagnosis of *** The pull method shortened the distance between the endoscope and the lesion and facilitated EUS-FNA of pancreatic head cancer. The push method contributed to the histological diagnosis of pancreatic head cancer using EUS-FNA specimens.

关键词： Endoscopic ultrasound-guided fine needle aspiration Pancreatic head Pancreatic cancer Push method Pull method

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Pancreatic neuroendocrine tumor Grade 1 patients followed up without surgery:Case series

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World Journal of Clinical Oncology 2017年第3期8卷 293-299页

作者： Mitsuru Sugimoto Tadayuki Takagi Rei Suzuki Naoki Konno Hiroyuki Asama Ko Watanabe Jun Nakamura Hitomi Kikuchi Yuichi Waragai Mika Takasumi Satoshi Kawana yuko Hashimoto Takuto Hikichi Hiromasa Ohira Department of Gastroenterology Fukushima Medical UniversitySchool of Medicine Department of Endoscopy Fukushima Medical University Hospital Department of Diagnostic Pathology Fukushima Medical UniversitySchool of Medicine

Among the three grades of neuroendocrine tumors(NETs),the prognosis for Grade 1(G1) with surgery is very ***,we evaluated the prognoses of pancreatic NET(PNET) G1 patients without surgery.A total of 8 patients who were diagnosed with NET G1,with an observation period of more than 6 mo until surgery or without surgery,were *** patients who underwent surgery were ultimately diagnosed using specimens obtained during the surgery,whereas the patients who did not undergo surgery were diagnosed using specimens obtained by endoscopic ultrasonography-guided fine needle ***,we mainly evaluated the observation period and tumor *** observation period for the five cases with surgery ranged from 6-80 mo,and tumor growth was observed in one *** contrast,the observation period for the three cases without surgery ranged from 17-54 mo,and tumor growth was not ***,although the first-choice treatment for NETs is surgery,our experience includes certain NET G1 patients who were followed up without surgery.

关键词： Pancreatic neuroendocrine tumors Metastasis Neuroendocrine tumors Grade 1 Follow-up Surgery

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Genetic Characterization of Streptococcus pyogenes emm89 Strains Isolated in Japan From 2011 to 2019

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Infectious Microbes & Diseases 2020年第4期2卷 160-166页

作者： Yujiro Hirose Masaya Yamaguchi Norihiko Takemoto Tohru Miyoshi-Akiyama Tomoko Sumitomo Masanobu Nakata Tadayoshi Ikebe Tomoki Hanada Takahiro Yamaguchi Ryuji Kawahara Rumi Okuno Hitoshi Otsuka yuko Matsumoto Yuji Terashima Yu Kazawa Noriko Nakanishi Kaoru Uchida Yumi Akiyama Kaori Iwabuchi Chikara Nakagawa Kazunari Yamamoto Victor Nizet Shigetada Kawabata Department of Oral and Molecular Microbiology Osaka University Graduate School of DentistrySuitaOsakaJapan Department of Pediatrics University of California at San Diego School of MedicineLa JollaCAUSA Department of Infectious Diseases Research InstituteNational Center for Global Health and MedicineTokyoJapan Department of Oral Microbiology Kagoshima University Graduate School of Medical and Dental SciencesKagoshimaJapan Department of Bacteriology I National Institute of Infectious DiseasesTokyoJapan Division of Microbiology Osaka Institute of Public HealthOsaka CityOsakaJapan Department of Microbiology Tokyo Metropolitan Institute of Public HealthTokyoJapan Department of Public Health Sciences Yamaguchi Prefectural Institute of Public Health and Environment Yamaguchi CityYamaguchiJapan Microbiological Testing and Research Division Yokohama City Institute of Public HealthYokohamaKanagawaJapan Department of Microbiology Fukushima Prefectural Institute of Public HealthFukushima CityFukushimaJapan Department of Infectious Diseases Kobe Institute of HealthKobeHyogoJapan Department of Bacteriology Toyama Institute of HealthImizuToyamaJapan Infectious Disease Research Division Hyogo Prefectural Institute of Public Health ScienceKakogawaHyogoJapan Department of Health Science Iwate Prefectural Research Institute for Environmental Sciences and Public HealthMoriokaIwateJapan Division of Microbiology Kyoto City Institute of Health and Environmental SciencesKyoto CityKyotoJapan Niigata City Institute of Public Health and the Environment Niigata CityNiigataJapan Skaggs School of Pharmaceutical Sciences University of California at San DiegoLa JollaCAUSA.

Invasive infection caused by Streptococcus pyogenes emm89 strains has been increasing in several countries linked to a recently emergent clade of emm89 strains,designated clade *** Japan,the features of emm89 *** strains,such as clade classification,remains *** this study,we collected emm89 strains isolated from both streptococcal toxic shock syndrome(STSS)(89 STSS isolates)and noninvasive infections(72 non-STSS isolates)in Japan from 2011 to 2019,and conducted wholegenome sequencing and comparative analysis,which resulted in classification of a large majority into clade 3 regardless of disease *** addition,invasive disease-associated factors were found among emm89 strains,including mutations of control of virulence sensor,and absence of the hylP1 gene encoding *** findings provide new insights into genetic features of emm89 strains.

关键词： clade 3 emm89 streptococcal toxic shock syndrome Streptococcus pyogenes

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Screening for hilar biliary invasion in ampullary cancer patients

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World Journal of Gastrointestinal Endoscopy 2022年第9期14卷 536-546页

作者： Tadayuki Takagi Mitsuru Sugimoto Rei Suzuki Naoki Konno Hiroyuki Asama Yuki Sato Hiroki Irie Jun Nakamura Mika Takasumi Minami Hashimoto Tsunetaka Kato Ryoichiro Kobashi Takumi Yanagita yuko Hashimoto Shigeru Marubashi Takuto Hikichi Hiromasa Ohira Department of Gastroenterology Fukushima Medical UniversityFukushima 960-1295Japan Department of Endoscopy Fukushima Medical University HospitalFukushima 960-1295Japan Department of Pathological Diagnosis Fukushima Medical UniversityFukushima 960-1295Japan Department of Hepato-Biliary-Pancreatic and Transplant Surgery Fukushima Medical UniversityFukushima 960-1295Japan

BACKGROUND The treatment for ampullary cancer is pancreatoduodenectomy or local ***,effective methods for the preoperative investigation of hilar biliary invasion in ampullary cancer patients have not yet been *** To determine the necessity of and an appropriate method for investigating hilar biliary invasion of ampullary *** Among 43 ampullary cancer patients,34 underwent endoscopic treatment(n=9)or surgery(n=25).The use of imaging findings(thickening and enhancement of the bile duct wall on contrast-enhanced computed tomography,irregularity on endoscopic retrograde cholangiography,thickening of the entire bile duct wall on intraductal ultrasonography(IDUS),and partial thickening of the bile duct wall on IDUS)and biliary biopsy results for diagnosing hilar biliary invasion of ampullary cancer was *** Hilar invasion was not observed in every *** the patients who did not undergo biliary stent insertion,the combination of partial thickening of the bile duct wall on IDUS and biliary biopsy results showed the highest accuracy(100%)for diagnosing hilar biliary ***,each imaging method and biliary biopsy yielded some false-positive *** Although some false-positive results were obtained with each method,the combination of partial thickening of the bile duct wall on IDUS and biliary biopsy results was useful for diagnosing hilar biliary invasion of ampullary ***,hilar invasion of ampullary cancer is rare;therefore,the investigation of hilar biliary invasion of ampullary cancer might be unnecessary.

关键词： Ampullary cancer Biliary biopsy Contrast-enhanced CT Hilar biliary invasion Intraductal ultrasonography

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