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Denaturation of DNA in Ternary Mixed Solution of Water/Hydrophilic/Hydrophobic Organic Solvent

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Journal of Analytical Sciences, Methods and Instrumentation 2017年第2期7卷 40-46页

作者： yuki Ito Kazuhiko Tsukagoshi Akira kobayashi Department of Chemical Engineering and Materials Science Doshisha University Kyotanabe Japan Bio-Microfluidic Science Research Center Doshisha University Kyotanabe Japan Department of Medical Life Systems Doshisha University Kyotanabe Japan

Denaturation was examined for the first time in a ternary mixed solution of water/hydrophilic/ hydrophobic organic solvent using λ-DNA and a plasmid as models. The absorbance of λ-DNA and the plasmid at 260 nm gradually increased for several days up to 1.68 and 1.38 times the initial values, respectively, in a water/acetonitrile/ethyl acetate (15:3:2, volume ratio) mixed solution, whereas there was little change in a water/acetonitrile (15:3, volume ratio) mixed solution. The plasmid treated with the ternary mixed solution was also examined with agarose gel electrophoresis. These experimental data indicated that λ-DNA changed from a double helix structure to a single helix structure and that the plasmid partially transformed to generate a denaturation bubble in the structure. The new idea of using the ternary mixed solution first enabled the interaction of the hydrophobic organic solvent (e.g., ethyl acetate) molecule with the double helical structure of DNA, leading to specific slow-proceeding denaturation.

关键词： Denaturation DNA Ternary Mixed Solvent Hydrophobic Organic Solvent Absorption Behavior

Feasible Resolution of Angular Displacement Measurement by an Optical Angle Sensor Based on Laser Autocollimation

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Nanomanufacturing and Metrology 2023年第4期6卷 46-59页

作者： Hyunsung Lim yuki Shimizu Division of Mechanical and Aerospace Engineering Hokkaido UniversitySapporoJapan

The feasible resolution of angular displacement measurement by an optical angle sensor based on laser autocollimation is *** the sensor sensitivity while maintaining the noise level of the sensor signal as low as possible is necessary to achieve high-resolution angular displacement *** this paper,the contribution of each component,such as a photodiode,a trans-impedance amplifer,and an analog-to-digital converter in the optical angle sensor,to the noise level of the sensor signal is frst estimated on the basis of theoretical *** feasible sensitivity of the optical angle sensor is also estimated in numerical *** sensitivity of a photodiode element at the edge of its photosensitive area is evaluated in experiments to realize the estimation of the angle sensor *** results are applied to the numerical *** infuences of the measurement laser beam diameter,the spot diameter of the focused laser beam on the photosensitive area,and the focal length of the collimator objective of the optical angle sensor are also consid-ered in the numerical ***,a prototype optical setup is *** are performed to demonstrate that a compact optical angle sensor based on laser autocollimation with a collimator objective having a focal length shorter than 100 mm can achieve a resolution beyond 0.001 arc-second with a bandwidth of 1 *** resolution is better than those achieved by commercial autocollimators employing an image sensor or a position-sensitive *** industrial contribution of this paper lies in the detailed breakdown of noise components in the readout signal of an angle sensor in a practical condition and the systematic estimation of its feasible resolution as well as its sensitivity.

关键词： Angle measurement Resolution Laser autocollimation Photodiode Signal noise

成纤维细胞体外培养结合串联质谱酰基肉碱谱诊断极长链酰基辅酶A脱氢酶缺陷症

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实用儿科临床杂志 2009年第20期24卷 1560-1562页

作者：李红马立燕马金海福田诚司长谷川有纪小林弘典山口清次宁夏医科大学附属医院儿科银川750004 日本岛根大学医学部小儿科日本岛根6938501

目的研究成纤维细胞体外培养结合串联质谱酰基肉碱谱分析对极长链酰基辅酶A脱氢酶(VLCAD)缺陷症的诊断价值。方法取健康对照标本6例,VLCAD缺陷症患儿标本9例(包括临床严重型2例,中间型3例及肌病型4例。男5例,女4例)。以棕榈酸(C16酸)为... 详细信息

目的研究成纤维细胞体外培养结合串联质谱酰基肉碱谱分析对极长链酰基辅酶A脱氢酶(VLCAD)缺陷症的诊断价值。方法取健康对照标本6例,VLCAD缺陷症患儿标本9例(包括临床严重型2例,中间型3例及肌病型4例。男5例,女4例)。以棕榈酸(C16酸)为培养溶媒底物在37℃培养箱中培养成纤维细胞株96h,应用串联质谱分析相应培养液中的酰基肉碱谱。结果与健康对照组比较,VLCAD缺陷症患儿乙酰肉碱(C2)明显降低,相应长链酰基肉碱异常增高,其中肉豆寇烯酰肉碱(C14)-酰基肉碱在临床严重型患儿中增高明显,月桂酰肉碱(C12)-酰基肉碱在临床轻型病例(包括中间型和肌病型)中增高更显著。长链酰基肉碱之和(ΣLC)及其与乙酰肉碱之比值(ΣLC/C2),VLCAD缺陷症患儿均明显高于健康对照。结论成纤维细胞体外特定条件培养结合串联质谱酰基肉碱谱分析,可诊断和鉴别VLCAD缺陷症及其临床分型,C12-酰基肉碱和C14-酰基肉碱可作为诊断标记。

关键词：极长链酰基辅酶A脱氢酶缺陷症串联质谱酰基肉碱成纤维细胞

Liver injury after aluminum potassium sulfate and tannic acid treatment of hemorrhoids

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World Journal of Gastroenterology 2017年第27期23卷 5034-5040页

作者： Kenichi Yoshikawa Reimi Kawashima yuki Hirose Keiko Shibata Takafumi Akasu Noriko Hagiwara Takeharu Yokota Nami Imai Akira Iwaku Go kobayashi Hirohiko kobayashi Akiyoshi Kinoshita Nao Fushiya Hiroyuki Kijima Kazuhiko Koike Masayuki Saruta Division of Gastroenterology and Hepatology Internal Medicinethe Jikei University Daisan Hospital Division of Gastroenterology and Hepatology Internal Medicinethe Jikei University School of Medicine

We are reporting a rare case of acute liver injury that developed after an internal hemorrhoid treatment with the aluminum potassium sulfate and tannic acid(ALTA) regimen. A 41-year-old man developed a fever and liver injury after undergoing internal hemorrhoid treatment with a submucosal injection of ALTA with lidocaine. The acute liver injury was classified clinically as hepatocellular and pathologically as cholestastic. We could not classify the mechanism of injury. High eosinophil and immunoglobulin E levels characterized the injury,and a drug lymphocyte stimulation test was negative on postoperative day 25. Fluid replacement for two weeks after hospitalization improved the liver injury. ALTA therapy involves injecting chemicals into the submucosa,from the rectum to the anus,and this is the first description of a case that developed a severe liver disorder after this treatment; hence,an analysis of future cases as they accumulate is desirable.

关键词： Aluminum potassium sulfate and tannic acid injection Aluminum potassium sulfate and tannic acid therapy Rectal submucosal injection Drug-induced lymphocyte stimulation test Drug-induced liver injury

Description of sand–metal friction behavior based on subloadingfriction model

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Friction 2022年第10期10卷 1660-1675页

作者： Toshiyuki OZAKI yuki YAMAKAWA Masami UENO Koichi HASHIGUCHI Kyushu Electric Engineering Consultants Inc. Fukuoka 810-0005Japan Department of Civil and Environmental Engineering Tohoku UniversitySendai 980-8579Japan University of the Ryukyus Okinawa 903-0213Japan MSC Software Ltd. Tokyo 101-0054Japan Kyushu University Fukuoka 819-0395Japan

A subloading-friction model is formulated to describe the smooth transient variation from static friction to kinetic friction,the recovery to static friction after the sliding velocity decreases,and the accumulation of sliding displacement under the cyclic loading of contact *** the past relevant studies,however,the model formulation used for simulations is limited to the hypoelastic-based plasticity framework,and the validation of the model is limited to simulations of the test data for metal-to-metal *** this study,the formulation of the subloading-friction model based on a hyperelastic-based plasticity framework is *** the fields of civil,geotechnical,agricultural engineering,and terramechanics,the interaction between soils and metals is critical,as reflected in construction and agricultural machinery,foundation piles,and retaining *** validity of the model for describing the friction between various sands and metals is verified by simulations of the experimental data under monotonic and cyclic loadings.

关键词： subloading-friction model sand-metal friction monotonic/cyclic sliding static/kinetic friction

Behavior of Vaneless Diffuser Stall in a Centrifugal Compressor

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Journal of Thermal Science 2022年第1期31卷 3-12页

作者： AGARI yuki YAMAO Yoshifumi FUJISAWA Nobumichi OHTA Yutaka Department of Applied Mechanics and Aerospace Engineering Waseda UniversityTokyo 169-8555Japan

The rotating stall in a centrifugal compressor with a vaneless diffuser was investigated both experimentally and numerically with focus on the effect of the internal flow field within the impeller on the diffuser *** numerical analysis,the boundary layer separation at the impeller outlet was found to play an important role in the expansion and rotation processes of the diffuser *** particular,the expanded boundary layer separation near the hub side at the outlet of the main blade(M.B.)suction surface passage was considered to be the main cause of the expansion and rotation processes.A longitudinal vortex existed at the throat of the ***,and the mass flow rate in the *** was significantly reduced by the blockage *** addition,the longitudinal vortex induced the rolling up flow near the hub side at the impeller ***,the boundary layer separation expanded.

关键词： centrifugal compressor vaneless diffuser pressure and velocity measurement CFD rotating stall

Psychosis symptoms following aberrant immunity in the brain

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Neural Regeneration Research 2021年第3期16卷 512-513页

作者： Akitoshi Ozaki yuki Yamawaki Gen Ohtsuki Department of Biophysics Kyoto University Graduate School of ScienceKyotoJapan The Hakubi Center for Advanced Research Kyoto UniversityKyotoJapan Department of Drug Discovery Medicine Kyoto University Graduate School of MedicineKyotoJapan

Neuroinflammation in the brain is thought related to the emergence of various psychoses,although the identifying regional significance,the involvement of immune-cells and lymphocytic activity,and ways for the therapeutic recovery are under the effort of *** recently revealed that the cerebellar acute inflammation causes the symptoms manifested in mood disorders or developmental disorders,which were associated with hyperexcitability due to immune-triggered plasticity and the overconnectivity between the inflamed cerebellum and prefrontal cortex(Yamamoto et al.,2019).

关键词： inflammation immunity involvement

Decreased accumulation of ultrasound contrast in the liver of nonalcoholic steatohepatitis rat model

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World Journal of Gastroenterology 2011年第37期17卷 4191-4198页

作者： yuki Miyata Takeo Miyahara Fuminori Moriyasu Department of Gastroenterology and Hepatology Tokyo Medical University

AIM:To investigate the diagnosis of nonalcoholic steatohepatitis(NASH) using contrast ultrasonography in the NASH rat ***:The liver in methionine choline-deficient diet(MCDD) rats,a NASH model constructed by feeding an MCDD,was examined by contrast ultrasonography at weeks 2,4,8,12 and 16,with late phase images of contrast ultrasonography(Kupffer imaging) in which contrast enhancement was achieved by incorporation of a contrast agent by Kupffer cells(KCs),and images were compared to those in rats taking a regular ***:Decrease in contrast enhancement was observed first in MCDD rats at week *** were counted based on immunohistochemistry,but their numbers were not reduced and it was assumed that attenuation of contrast enhancement was attributable to reduced phagocytic activity of the ***:It is suggested that clinical application of contrast ultrasonography may be valuable for noninvasive diagnosis of NASH.

关键词： Nonalcoholic steatohepatitis Leptin Kupffer cell Methionine choline-deficient diet Contrast ultrasound

Global Optimum-Based Search Differential Evolution

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IEEE/CAA Journal of Automatica Sinica 2019年第2期6卷 379-394页

作者： Yang Yu Shangce Gao Yirui Wang yuki Todo the Faculty of Engineering University of Toyama the Faculty of Electrical and Computer Engineering Kanazawa University

In this paper, a global optimum-based search strategy is proposed to alleviate the situation that the differential evolution(DE) usually sticks into a stagnation, especially on complex problems. It aims to reconstruct the balance between exploration and exploitation, and improve the search efficiency and solution quality of DE. The proposed method is activated by recording the number of recently consecutive unsuccessful global optimum updates. It takes the feedback from the global optimum,which makes the search strategy not only refine the current solution quality, but also have a change to find other promising space with better individuals. This search strategy is incorporated with various DE mutation strategies and DE variations. The experimental results indicate that the proposed method has remarkable performance in enhancing search efficiency and improving solution quality.

关键词： Differential evolution(DE) global optimum memetic algorithm