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Granulocyte Colony-Stimulating Factor Enhances the Anticancer Effects of Cisplatin against Lung Cancer by Promoting Angiogenesis

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Advances in Lung Cancer 2017年第1期6卷 1-11页

作者： Yun-Mo Li Yasushi Ohno Norihiko Funaguchi Takenobu Gomyo yuka sasaki Sayaka Toyoshi Daizo Kaito Komei Yanase Junki Endo Fumitaka Ito Masanori Kawasaki Shinya Minatoguchi Department of Cardiology and Respirology Graduate School of Medicine Gifu University Gifu Japan Department of Respiratory Medicine Murakami Memorial Hospital Asahi University Mizuho Japan

The G-CSF is used as a therapeutic drug of the febrile neutropenia in lung cancer chemotherapy, however, there were few reports that showed the effects of combination effects of G-CSF and anticancer drugs against lung cancer. In the present study, we investigated the effects of G-CSF and the combination effects of G-CSF and cisplatin on lung cancer growth. We investigated the effect of G-CSF against the LL-2 and KLN-205 cells by MTT assay and tried to detect the G-CSF receptor by RT-PCR. Next, to analyze the G-CSF effects in vivo, we transplanted the LL-2 into C57BL/6 mice, intraperitoneally administered G-CSF (30 micro/kg/day) with or without cisplatin (5 mg/kg), measured the tumor size and analyzed pathologically by HE and immunostaining. In vitro analyses, G-CSF showed no effects in LL-2 and KLN-205 cells, and RT-PCR revealed no G-CSF receptor mRNA. In vivo analyses, G-CSF alone did not significantly suppress tumor growth. However, concurrent G-CSF administration with cisplatin significantly enhanced the tumor suppressing effect of cisplatin in early stage of tumor growth. The analysis data of vWF immunostaining indicated that the neovascularization in the peripheral region of the tumors was more enhanced in G-CSF treatment mice. ELISA assay revealed that G-CSF did not influence the serum concentration of TNF-alpha and IL-12 in tumor-bearing mice. This study suggests that concurrent (combination) administration of cisplatin with G-CSF is a safe and effective method for enhancing anticancer effects and reducing chemotherapeutic agent-induced myelosuppression.

关键词： Granulocyte Colony-Stimulating Factor Lung Cancer G-CSF Receptor Angiogenesis Anticancer Agent Cisplatin TNF-Alpha IL-12

几种新型有机肥对菜用毛豆产量、品质及化肥氮利用率的影响

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浙江大学学报（农业与生命科学版） 2008年第3期34卷 289-295页

作者：叶静安藤丰符建荣佐佐木由佳角田宪一浙江省农业科学院环境资源与土壤肥料研究所浙江杭州310021 日本山形大学农学部日本鹤冈997-8555

通过田间试验研究4种有机废弃物中的KEON(KCl可提取态氮)和PEON(磷酸缓冲液可提取态氮)2种形态的有机氮对菜用毛豆生长及化肥氮利用率的影响.结果表明:施用鸡粪和豆粕混合肥与单施化肥相比,地上部分干物质重分别提高20·4%和33·2%,籽... 详细信息

通过田间试验研究4种有机废弃物中的KEON(KCl可提取态氮)和PEON(磷酸缓冲液可提取态氮)2种形态的有机氮对菜用毛豆生长及化肥氮利用率的影响.结果表明:施用鸡粪和豆粕混合肥与单施化肥相比,地上部分干物质重分别提高20·4%和33·2%,籽粒中的蛋白质含量分别提高17·8%和16·2%,达到显著差异水平;与单施化肥相比,毛豆产量分别提高38·0%和32·5%.有机肥中的KEON和PEON与化学氮肥利用率呈正相关,其相关系数分别达到0·88和0·87(P<0·05).与单施化肥相比,施用鸡粪和豆粕混合肥的当季化学氮肥利用率可分别提高94·9%和67·4%.

关键词：菜用毛豆有机肥 ^15N同位素氮肥利用率

不同有机肥对土壤中的氮素矿化及对化肥氮固持的影响

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浙江农业学报 2008年第3期20卷 176-180页

作者：叶静安藤丰符建荣佐佐木由佳角田宪一浙江省农业科学院环境资源与土壤肥料研究所浙江杭州310021 日本山形大学农学部日本鹤冈997-8555

采用15N示踪法研究不同有机肥品种在土壤中的氮素矿化释放规律及对化肥氮固定的影响。结果表明,培养90 d后,鸡粪、豆粕米糠混合肥氮素矿化量分别达到了95.6和185.4 mg/kg,而牛粪和污泥堆肥分别为-28.1和-10.9 mg/kg。4种有机肥在化肥施... 详细信息

采用15N示踪法研究不同有机肥品种在土壤中的氮素矿化释放规律及对化肥氮固定的影响。结果表明,培养90 d后,鸡粪、豆粕米糠混合肥氮素矿化量分别达到了95.6和185.4 mg/kg,而牛粪和污泥堆肥分别为-28.1和-10.9 mg/kg。4种有机肥在化肥施入土壤后,对化肥氮在土壤中的保留进行了观察,鸡粪和豆粕米糠混合肥对化肥氮的固持率明显高于牛粪和污泥堆肥。

关键词：有机肥氮素矿化 ^15N同位素 KEON PEON

东亚地区大豆(Glycine max)考古遗存的研究——大小是否重要?

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东方考古 2014年第1期 311-331,549页

作者：李炅娥加里克劳福德刘莉佐佐木由香陈雪香杨谦(译) 俄勒冈大学人类学系美国多伦多大学人类学系加拿大斯坦福大学东亚语言与文化系美国古代实验室有限公司日本山东大学考古系中国

一、简介大豆(Glycine max ***)不仅是世界上最重要的油料作物之一,也是禽畜主要的蛋白质来源①,其产量在全球所有作物中排名第七②。尽管这种作物对世界经济的重要性不言而喻,然而,它是如何成为一种重要的作物,并最终成为栽培作物的等... 详细信息

一、简介大豆(Glycine max ***)不仅是世界上最重要的油料作物之一,也是禽畜主要的蛋白质来源①,其产量在全球所有作物中排名第七②。尽管这种作物对世界经济的重要性不言而喻,然而,它是如何成为一种重要的作物,并最终成为栽培作物的等一系列问题尚未得到有效解决。生物遗传学和历史文献研究得出的大豆演化历史表明,大豆是在东亚地区最先栽培起来的,并在周代(距今2500年左右)时已经成为中国的一项重要作物。然而,大豆是在何时何地以及在什么背景下变得与人类密不可分,尚不明了。

关键词：研究栽培大豆历史文献作物东亚地区禽畜蛋白质

Transcriptional regulation of human polo-like kinases and early mitotic inhibitors

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Journal of Genetics and Genomics 2008年第4期35卷 215-224页

作者： Moe Tategu Hiroki Nakagawa Kaori sasaki Rieko Yamauchi Sota Sekimachi yuka Suita Naoko Watanabe Kenichi Yoshida Department of Life Sciences Meiji University School of Agriculture 1-1-1 Higashimita Tama-ku Kawasaki Kanagawa 214-8571 Japan

Human polo-like kinases （PLK1-PLK4） have been implicated in mitotic regulation and carcinogenesis. PLK1 phosphorylates early mitotic inhibitor 1 （Emil） to ensure mitosis entry, whereas Emi2 plays a key role during the meiotic cell cycle. Transcription factor E2F is primarily considered to regulate the G1/S transition of the cell cycle but its involvement in the regulation of mitosis has also been recently suggested. A gap still exists between the molecular basis of E2F and mitotic regulation. The present study was designed to characterize the transcriptional regulation of human PLK and Emi genes. Adenoviral overexpression of E2F1 increased PLK1 and PLK3 mRNA levels in A549 cells. A reporter gene assay revealed that the putative promoter regions of PLK1, PLK3, and PLK4 genes were responsive to activators E2F, E2F1-E2F3. We further characterized the putative promoter regions of Emil and Emi2 genes, and these could be regulated by activators E2F and E2F1-E2F4, respectively. Finally, PLK1-PLK4, Emil, and Emi2 mRNA expression levels in human adult, fetal tissues, and several cell lines indicated that each gene has a unique expression pattern but is uniquely expressed in common tissues and cells such as the testes and thymus. Collectively, these results indicate that E2F can integrate G1/S and G2/M to oscillate the cell cycle by regulating mitotic genes PLK and Emi, leading to determination of the cell fate.

关键词： polo-like kinase early mitotic inhibitor cell cycle E2F promoter gene regulation

The Role of Thymidylate Synthase in Pemetrexed-Resistant Malignant Pleural Mesothelioma Cells

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Journal of Cancer Therapy 2013年第6期4卷 1052-1059页

作者： Tohru Obata Motohiro Tanaka yuka Suzuki Takuma sasaki Laboratory of Bioorganic Chemistry School of Pharmacy Aichi Gakuin University Nagoya Japan

We established new pemetrexed-resistant cells originating from malignant pleural mesothelioma MSTO-211H cells to clarify the mechanism involved in pemetrexed resistance in malignant pleural mesothelioma. In the pemetrexed-resistant cells, only thymidylate synthase (TYMS) mRNA was overexpressed among other well-known molecular targets and chemosensitivity determinants of pemetrexed, and the role of the TYMS gene was ascertained by artificial regulation induced by specific siRNA. Silencing the TYMS expression partially restored the cytotoxicity of pemetrexed. The resistant cells did not display other gene alterations related to folate metabolism. We conclude that the primary mechanism imparting resistance to these cells is specific up-regulation of TYMS function. Further, the TYMS gene may serve as a useful biomarker for the prediction of pemetrexed chemosensitivity in patients with malignant pleural mesothelioma. We also investigated the efficacy of 1-(3-C-ethynyl-β-D-ribo-pento furanosyl)cytosine (ECyd) in overcoming pemetrexed resistance;this compound is presently undergoing clinical trials in the USA as TAS-106. ECyd had a similar antitumor effect on the resistant cells as that on the parental cells. In the clinical treatment of malignant pleural mesothelioma, ECyd promises to emerge as a novel drug.

关键词： Malignant Pleural Mesothelioma Thymidylate Synthase Pemetrexed ECyd

Food Intake Characteristics during Early Pregnancy in Women with Gestational Diabetes Mellitus

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Health 2017年第12期9卷 1711-1719页

作者： Atsuko Satoh Chikako Kishi Sangun Lee Masumi Saitoh Miwa Miura yuka Ohnuma Chizu Yamazaki Hidetada sasaki Department of Nursing Hirosaki University of Health and Welfare Aomori Japan Kensei Hospital Aomori Japan Department of Physical Therapy Aomori University of Health and Welfare Aomori Japan Sendai Tomizawa Hospital Sendai Japan

To investigate the characteristic food intake during early pregnancy in women with gestational diabetes mellitus (GDM) in a rural city in Aomori Prefecture, Japan, one hundred and twenty-one women were recruited and queried about their habitual dietary intake. Food intake of patients was assessed using the model nutritional balance chart at 12 - 16, 24 - 28, and 34 - 36 weeks of gestation. Of the 121 pregnant women examined, 19 were obese. During early pregnancy, food intake ratios of the obese women were significantly lower than those of the non-obese women for the categories of milk (p < 0.001) and sugar (p < 0.05). GDM group was 7 women among 19 women in obesity group during mid-pregnancy. During early pregnancy, women with GDM had significantly higher sugar intake ratios than women without GDM (p < 0.05). These results suggested that obese pregnant women are able to prevent GDM by limiting their sugar intake during early pregnancy.

关键词： Gestational Diabetes Mellitus Food Intake Characteristics Sugar Intake Obesity Early Pregnancy

Optimal Grip Span for Measuring Maximum Handgrip Strength in Preschool Children

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International Journal of Clinical Medicine 2022年第11期13卷 479-488页

作者： Takashi Abe Rika Sanui Arisa sasaki Aki Ishibashi Nanami Daikai yuka Shindo Akemi Abe Jeremy P. Loenneke Graduate School of Health and Sports Science & Institute of Health and Sports Science and Medicine Juntendo University Inzai-shi Chiba Japan Division of Children’s Health and Exercise Research Institute of Trainology Fukuoka-shi Fukuoka Japan Child Health Research Group Atagohama Kindergarten Fukuoka-shi Fukuoka Japan Department of Health Exercise Science and Recreation Management Kevser Ermin Applied Physiology Laboratory The University of Mississippi University MS USA

Background: Since the minimum grip span of a standard Smedley hand dynamometer is 4 cm, the handgrip strength (HGS) value at a less than 4 cm grip span has not been reported. Objective: The present study examined the impact of grip span on handgrip strength (HGS) in young children using a dynamometer ranging from 2 to 4.5 cm of grip span. Methods: A total of 93 (39 girls and 54 boys) children were recruited from a local kindergarten with the cooperation of their parents. Each participant performed one test trial and then five maximal trials using different grip spans (2.0, 3.0, 3.5, 4.0, and 4.5 cm) in random order and allowing a 1-minute rest between measures. Results: There was a statistically significant difference in HGS depending on which grip span was used, χ2(4) = 211.5 (p Conclusion: Our results recommended that the target grip span for measuring maximal HGS in young children is about 4 cm (4 ± 0.5 cm).

关键词： Childhood Grip Span Grip Strength Hand Morphology Reliability

Cisplatin Inhibits AhR Activation

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American Journal of Molecular Biology 2018年第1期8卷 69-82页

作者： Erina sasaki-Kudoh Ikuru Kudo yuka Kakizaki Miki Hosaka Shun-Ichi Ikeda Seiya Uemura Ewa Grave Shuntaro Togashi Taku Sugawara Hiroaki Shimizu Hideaki Itoh Department of Neurosurgery Akita University Graduate School of Medicine Akita Japan Department of Life Science Akita University Graduate School of Engineering Science Akita Japan Department of Spinal Cord and Spine Surgery Research Institute for Brain and Blood Vessels-Akita Akita Japan

The AhR binds to contain ligands, such as 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin, 3-methylcholantrene, or β-naphthoflavone. The activation mechanism of AhR is not yet fully understood, but it is known that AhR associates with the molecular chaperone HSP90 in the cytoplasm. There are a few reports about the association or dissociation of AhR and HSP90, and which domain of HSP90 binds to AhR. We reported the association and activation mechanisms between HSP90 and AhR-PAS or AhR-bHLH. In the current study, we found that cisplatin inhibits the AhR activation. Although ATP and 17-DMAG have no effect on the dissociation of HSP90 from AhR, some contents of HSP90 were dissociated from AhR in the presence of cisplatin. We could detect the increase of CYP1A in the presence of 3-MC. On the contrary, the induction of CYP1A1 was inhibited in the presence of cisplatin. We couldn’t detect AhR in the HeLa cell soluble fraction in the presence of 50 μM cisplatin. In the presence of MG-132, we could detect AhR. These results suggested that AhR was dissociated from the HSP90 chaperone complex and processed during the protein proteasome degradation system in the presence of cisplatin.

关键词： Cisplatin CDDP AhR Aryl Hydrocarbon Receptor HSP90 17-DMAG