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Cortical Organoid-on-a-Chip with Physiological Hypoxia for Investigating Tanshinone IIA-Induced Neural Differentiation

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Research 2024年第3期2023卷 457-468页

作者： Yue Zhi yujuan zhu Jinglin Wang Junqi Zhao Yuanjin Zhao Department of Rheumatology and Immunology Nanjing Drum Tower HospitalClinical Medical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing210023China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China Shenzhen Research Institute Southeast UniversityShenzhen518038China

Cortical organoids represent cutting-edge models for mimic human brain development during the early and even middle stage of pregnancy, while they often fail to recreate the complex microenvironmental factors, such as physiological hypoxia. Herein, to recapitulate fetal brain development, we propose a novel cortical organoid-on-a-chip with physiological hypoxia and further explore the effects of tanshinone IIA (Tan IIA) in neural differentiation. The microfluidic chip was designed with a micropillar array for the controlled and efficient generation of cortical organoids. With low oxygen, the generated cortical organoids could recapitulate key aspects of early-gestational human brain development. Compared to organoids in normoxic culturing condition, the promoted neurogenesis, synaptogenesis and neuronal maturation were observed in the present microsystem, suggesting the significance of physiological hypoxia in cortical development. Based on this model, we have found that Chinese herbal drug Tan IIA could promote neural differentiation and maturation, indicating its potential therapeutic effects on neurodevelopmental disorders as well as congenital neuropsychiatric diseases. These results indicate that the proposed biomimetic cortical organoid-on-a-chip model with physiological hypoxia can offer a promising platform to simulate prenatal environment, explore brain development, and screen natural neuroactive components.

关键词： promoted herbal cutting

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A commentary of“Mechanism of alkaline tolerance and application in crop improvement”:Top 10 Chinese Scientific Advances of 2023,China

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Fundamental Research 2024年第3期4卷 697-698页

作者： yujuan zhu Sanwen Huang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518120China State Key Laboratory of Tropical Crop Breeding Chinese Academy of Tropical Agricultural SciencesHaikouHainan 571101China

As global warming accelerates,soil degradation,exacerbated by extreme weather,has become a significant environmental issue ***,in particular,is grappling with a yearly reduction in arable land due to soil degradation,acidification,and salinization,posing potential threats to food *** country has a vast area of saline-alkaline land,over half of which are high-pH soda saline *** lands often experience simultaneous salt and alkali stress,severely inhibiting plant growth and crop ***,finding ways to utilize this marginal and saline-alkaline land for food production is a critical scientific *** this context,Chinese scientists have achieved a breakthrough towards harnessing saline-alkaline land.

关键词： alkaline utilize breakthrough

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Emerging microfluidics for the modeling and treatment of arthritis

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Engineered Regeneration 2024年第2期5卷 153-169页

作者： Nengjie Yang Chi Sun Chen Dong Yuting Huang yujuan zhu Zhifeng Gu Department of Rheumatology Research Center of ImmunologyAffiliated Hospital of Nantong UniversityMedical School of Nantong UniversityNantong 226001China Department of Geriatrics Affiliated Hospital of Nantong UniversityNantong UniversityNantong 226001China Department of Dermatology Affiliated Hospital of Nantong UniversityMedical School of Nantong UniversityNantong 226001China Research Center of Clinical Medicine Research Center of ImmunologyAffiliated Hospital of Nantong UniversityNantong 226001China

Microfluidic is a technology that allows the precise control of fluid in a *** its advantages of high throughput and low cost,microfluidic technology has achieved good performance in various fields in recent *** is a general term for a variety of joint diseases,which can be clinically manifested as joint pain and swelling,seriously affecting people’s physical and mental *** present,the causes of arthritis disease are still unknown,and existing disease models and treatment methods are still limited,so more treatments need to be *** organ chip is a cutting-edge technology to build a bionic human organ model,which can reflect the structure and function characteristics of human organs by simulating the physiological environment of tissues and cells in *** paper reviews the application of microfluidic technology in the modeling and treatment of arthritis,hoping to open up a new vision for the study of arthritis.

关键词： Microfluidics Arthritis Organ on a chip Drug delivery system Drug screening

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Ca^(2+)-independent ZmCPK2 is inhibited by Ca^(2+)-dependent ZmCPK17 during drought response in maize

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Journal of Integrative Plant Biology 2024年第7期66卷 1313-1333页

作者： Xiaoying Hu Jinkui Cheng Minmin Lu Tingting Fang yujuan zhu Zhen Li Xiqing Wang Yu Wang Yan Guo Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Environmental Resilience Frontiers Science Center for Molecular Design BreedingCenter for Crop Functional Genomics and Molecular BreedingCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 071002China

Calcium oscillations are induced by different ***-dependent protein kinases(CDPKs/CPKs)are one major group of the plant calcium decoders that are involved in various processes including drought *** CPKs are ***,we identified ZmCPK2 as a negative regulator of drought resistance by screening an overexpression transgenic maize *** found that ZmCPK2 does not bind calcium,and its activity is mainly inhibited during short term abscisic acid(ABA)treatment,and dynamically changed in prolonged ***,ZmCPK2 interacts with and is inhibited by calcium-dependent ZmCPK17,a positive regulator of drought resistance,which is activated by ***17 could prevent the nuclear localization of ZmCPK2 through phosphorylation of ***2 interacts with and phosphorylates and activates ZmYAB15,a negative transcriptional factor for drought *** results suggest that drought stress-induced Ca^(2+)can be decoded directly by ZmCPK17 that inhibits ZmCPK2,thereby promoting plant adaptation to water deficit.

关键词： CDPK drought response phosphorylation stomatal movement transcriptional regulation

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Sensors-integrated organ-on-a-chip for biomedical applications

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Nano Research 2023年第7期16卷 10072-10099页

作者： Hanxu Chen Zhiqiang Luo Xiang Lin yujuan zhu Yuanjin Zhao Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001China

As a promising new micro-physiological system,organ-on-a-chip has been widely utilized for in vitro pharmaceutical study and tissues engineering based on the three-dimensional constructions of tissues/organs and delicate replication of in vivo-like *** better observe the biological processes,a variety of sensors have been integrated to realize in-situ,realtime,and sensitive monitoring of critical signals for organs development and disease ***,we discuss the recent research advances made with respect to sensors-integrated organ-on-a-chip in this overall ***,we briefly explore the underlying fabrication procedures of sensors within microfluidic platforms and several classifications of sensory ***,emphasis is put on the highlighted applications of different types of organ-on-a-chip incorporated with various *** but not least,perspective on the remaining challenges and future development of sensors-integrated organ-on-a-chip are presented.

关键词： organ-on-a-chip(OOC) micro-physiological system tissue engineering sensors biomedical

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ARF2 coordinates with PLETHORAs and PINs to orchestrate ABA-mediated root meristem activity in Arabidopsis

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Journal of Integrative Plant Biology 2017年第1期59卷 30-43页

作者： Sujittra Promchuea yujuan zhu Zhizhong Chen Jing Zhang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China National Center for Plant Gene Research Beijing 100193China

Multiple hormones, including abscisic acid（ABA） and auxin, regulate cell division and differentiation of Arabidopsis root meristems. AUXIN RESPONSE FACTOR2（ARF2） functions as a negative regulator of ABA responses, as seed germination and primary root growth of arf2 mutants are hypersensitive to ABA. In this study, we found that ABA treatment reduced the expression levels of the PIN-FORMEDs（PIN） auxin efflux carriers, PIN1, PIN3,PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type. Also, arf2-101 pin1 and arf2-101 pin4 double mutants show less ABA-induced inhibition of root meristem activity than the arf2-101 mutants. Furthermore, ARF2 positively mediates the transcripts of transcription factor PLETHORA 1（PLT1）gene but negatively mediates PLT2 at protein level in root meristems. Using a dexamethasone（DEX）-inducible transgenic line, Pro35S：PLT2-GR, we showed that PLT2 greatly promotes cell division and completely inhibits cell differentiation in root meristems of the arf2-101 mutant once PLT2 is induced by DEX, which can be partially reversed by ABA treatment, suggesting that ABA regulates root meristem activity in both ARF2-dependent and independent pathways. Our results uncover a complex regulatory architecture in which ARF2 coordinates with PLTs and PINs to orchestrate ABA-mediated regulation of root meristem activity in Arabidopsis.

关键词： Arabidopsis coordinates mutant auxin hypersensitive transgenic transcripts germination partially regulate

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延续性冰刺激联合心理护理对改善脑梗死后合并吞咽困难患者吞咽功能的影响

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当代医学 2024年第4期30卷 169-172页

作者：王慧昂红丁静静朱玉娟皖南医学院第一附属医院神经内科安徽芜湖241000

目的探究延续性冰刺激联合心理护理在脑梗死后吞咽困难患者中的应用效果。方法选取2020年6月至2021年6月皖南医学院第一附属医院收治的89例脑梗死合并吞咽困难患者作为研究对象,根据护理方法不同分为实验组(n=43)与对照组(n=46)。对照... 详细信息

目的探究延续性冰刺激联合心理护理在脑梗死后吞咽困难患者中的应用效果。方法选取2020年6月至2021年6月皖南医学院第一附属医院收治的89例脑梗死合并吞咽困难患者作为研究对象,根据护理方法不同分为实验组(n=43)与对照组(n=46)。对照组接受常规护理及康复训练,实验组在对照组基础上接受延续性冰刺激联合心理护理。比较两组干预前后洼田饮水试验(WST)和标准吞咽功能评估量表(SSA)、汉密尔顿抑郁量表(HAMD)、焦虑自评量表(SAS)评分。结果干预前,两组WST分级和SSA评分比较差异无统计学意义;干预后,两组WST3~5级比例及SSA评分均低于干预前,且实验组低于对照组,差异有统计学意义(P<0.05)。干预前,两组HAMD、SAS评分比较差异无统计学意义;干预后,两组HAMD、SAS评分均低于干预前,且实验组低于对照组,差异有统计学意义(P<0.05)。结论延续性冰刺激联合心理护理可改善脑梗死合并吞咽困难患者的吞咽功能,并缓解其不良情绪。

关键词：脑梗死吞咽困难冰刺激心理护理延续护理模式

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安徽省流体观测井水温测项观测效能评价与分析

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科技资讯 2024年第16期22卷 225-228页

作者：朱玉娟车濛琪方震朱厚林吴婉楠大别山地震监测预报实验场安徽六安237000 六安地震监测中心站安徽六安237000 安徽蒙城地球物理国家野外科学观测研究站安徽蒙城233500 蒙城地震监测中心站安徽蒙城233500 地下结构探测与震灾风险防范安徽省重点实验室(筹) 安徽合肥230031

水温观测数据主要反映地热场的变化,数据产品服务于安徽省及邻区中、短、临地震预测与科学研究。全面梳理和分析安徽省地下流体水温测项的观测效能,一方面为改进安徽省水温测项的观测技术、提升观测质量提供依据;另一方面为流体异常核... 详细信息

水温观测数据主要反映地热场的变化,数据产品服务于安徽省及邻区中、短、临地震预测与科学研究。全面梳理和分析安徽省地下流体水温测项的观测效能,一方面为改进安徽省水温测项的观测技术、提升观测质量提供依据;另一方面为流体异常核实、异常分析和异常性质的判定提供参考,为科学研判安徽及其邻区的震情形势提供科学依据。

关键词：地下流体水温效能评估观测质量

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Bamboo‑Inspired Gasotransmitter Microfibres for Wound Healing

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Advanced Fiber Materials 2023年第1期5卷 388-399页

作者： Cheng Zhao Jiahui Guo Han Zhang Min Nie Caihong Yang yujuan zhu Yuanjin Zhao Liping Zhong Department of Rheumatology and Immunology Institute of Translational MedicineThe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210002China National Center for International Biotargeting Theranostics Guangxi Key Laboratory of Biotargeting TheranosticsCollaborative Innovation Center for Targeting Tumour TheranosticsGuangxi Medical UniversityGuangxi 530021China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Diabetic wounds have become a major clinical problem that cannot be ***,such as hydrogen sulphide(H_(2)S),have demonstrated value in inducing angiogenesis and accelerating wound healing,while their effective delivery is still ***,inspired by the continuous-independent hollow structure of bamboo,we propose novel gasotransmitter microfibres with septal H_(2)S bubbles using microfluidic spinning for diabetic wound *** from the exact control of microfluidics,gasotransmitter microfibres with different bubble sizes and morphologies could be generated successfully and *** the dual effects of drugs in the shell and gas in the core,the wound healing process could be ***,the controllable release of drugs could be achieved by adding responsive materials into the microfiber shell,which would promote continuous effects of contents on *** on in vitro and in vivo stud-ies,we have proven that these gasotransmitter microfibres have a positive impact on inducing angiogenesis and promoting cell proliferation during wound ***,it is believed that the bamboo-inspired gasotransmitter microfibres will have important value in gasotransmitter research and clinical applications.

关键词： Microfluidics Hydrogel Wound healing Bioinspired Gasotransmitter

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Neurodevelopmental impairment induced by prenatal valproic acid exposure shown with the human cortical organoid-on-a-chip model

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Microsystems & Nanoengineering 2020年第1期6卷 754-767页

作者： Kangli Cui Yaqing Wang yujuan zhu Tingting Tao Fangchao Yin Yaqiong Guo Haitao Liu Fei Li Peng Wang Yuejun Chen Jianhua Qin Division of Biotechnology CAS Key Laboratory of SSACDalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian 116023China University of Chinese Academy of Sciences Beijing 100049China CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina

Prenatal exposure to environmental insults can increase the risk of developing neurodevelopmental *** of the antiepileptic drug valproic acid(VPA)during pregnancy is tightly associated with a high risk of neurological disorders in ***,the lack of an ideal human model hinders our comprehensive understanding of the impact of VPA exposure on fetal brain development,especially in early ***,we present the first report indicating the effects of VPA on brain development at early stages using engineered cortical organoids from human induced pluripotent stem cells(hiPSCs).Cortical organoids were generated on micropillar arrays in a controlled manner,recapitulating the critical features of human brain development during early *** VPA exposure,cortical organoids exhibited neurodevelopmental dysfunction characterized by increased neuron progenitors,inhibited neuronal differentiation and altered forebrain *** analysis showed new markedly altered genes(e.g.,KLHL1,LHX9,and MGARP)and a large number of differential expression genes(DEGs),some of which are related to *** particular,comparison of transcriptome data via GSEA and correlation analysis revealed the high similarity between VPA-exposed organoids with the postmortem ASD brain and autism patient-derived organoids,implying the high risk of autism with prenatal VPA exposure,even in early *** new findings facilitate a better understanding of the cellular and molecular mechanisms underlying postnatal brain disorders(such as autism)with prenatal VPA *** established cortical organoid-on-a-chip platform is valuable for probing neurodevelopmental disorders under environmental exposure and can be extended to applications in the study of diseases and drug testing.

关键词： markedly inhibited exposure

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