检索结果-南通市图书馆

A mesenchymal-like subpopulation in non-neuroendocrine SCLC contributes to metastasis

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2021年第7期48卷 571-581页

作者： yujuan jin Tian Xiao Yan Feng jinhua Yang Chenchen Guo Liang Hu Hongbin Ji State Key Laboratory of Cell Biology Chinese Academy of SciencesShanghai 200031China Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of SciencesShanghai 200031China Center for Excellence in Molecular Cell Science Chinese Academy of SciencesShanghai 200031China Shenzhen Key Laboratory of Translational Medicine of Tumor Department of Cell Biology and GeneticsShenzhen University Health Sciences CenterShenzhenGuangdong 518060China University of Chinese Academy of Sciences Beijing 100049China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

Small cell lung cancer(SCLC)is the most aggressive lung cancer with high *** SCLC cells derived from the Rb1L/L/Trp53L/L(RP)autochthonous mouse model grew as adhesion or suspension in cell culture,and the adhesion cells are defined as non-neuroendocrine(non-NE)SCLC ***,we uncover the heterogenous subpopulations within the non-NE cells and referred to them as mesenchymallike(Mes)and epithelial-like(Epi)SCLC *** Mes cells have increased capability to form colonies in soft agar and harbored stronger metastatic capability in vivo when compared with the Epi *** Set Enrichment Analysis reveals that the transforming growth factor(TGF)-βsignaling is enriched in the Mes ***,inhibition of the TGF-βsignaling through ectopic expression of dominant-negative Tgfbr2(Tgfbr2-DN)or treatment with Tgfbr1 inhibitor SD-208 consistently abrogates tumor metastasis in nude mouse allograft ***,genetic deletion of Tgfbr2 or Smad4,the key components of the TGF-βsignaling pathway,dramatically attenuates SCLC metastasis in the RP autochthonous mouse ***,our results uncover the high heterogeneity in non-NE SCLC cells and highlight an important role of TGF-βsignaling in promoting SCLC metastasis.

关键词： Small cell lung cancer Heterogeneity Metastasis TGF-b signaling

PI3K/Akt/mTOR signaling orchestrates the phenotypic transition and chemo-resistance of small cell lung cancer

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2021年第7期48卷 640-651页

作者： Xuefeng Li Cheng Li Chenchen Guo Qiqi Zhao Jiayu Cao Hsin-Yi Huang Meiting Yue Yun Xue yujuan jin Liang Hu Hongbin Ji State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Department of Medical Oncology The First Affiliated HospitaHengyang MedicalSchoolUniversity of South ChinaHengyangHunan 421001China School of Life Science and Technology Shanghai Tech UniversityShanghai 200120China CAS Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

Small cell lung cancer(SCLC)is a phenotypically heterogeneous disease with an extremely poor prognosis,which is mainly attributed to the rapid development of resistance to ***,the relation between the growth phenotypes and chemo-resistance of SCLC remains largely *** comprehensive bioinformatic analyses,we found that the heterogeneity of SCLC phenotype was significantly associated with different sensitivity to *** or semiadherent SCLC cells were enriched with activation of the PI3K/Akt/mTOR pathway and were highly ***,activation of the PI3K/Akt/mTOR pathway promotes the phenotypic transition from suspension to adhesion growth pattern and confers SCLC cells with *** chemo-resistance could be largely overcome by combining chemotherapy with PI3K/Akt/mTOR pathway *** findings support that the PI3K/Akt/mTOR pathway plays an important role in SCLC phenotype transition and chemo-resistance,which holds important clinical implications for improving SCLC treatment.

关键词： SCLC Heterogeneity Growth pattern Phenotype transition Drug resistance PI3K/Akt/mTOR pathway

TAZ inhibits SCLC metastasis through GALNT18-mediated O-glycosylation

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2024年第11期51卷 1314-1317页

作者： Zhiwei Zhang Zhijue Xu Xinyi Qian Yanxu Chen Duo Li Zhen Qin Luonan Chen Yan Zhang yujuan jin Hongbin Ji State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCAS Center for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Systems Biomedicine(Ministry of Education) Shanghai Center for Systems BiomedicineShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China Key Laboratory of Systems Biology Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhouZhejiang 330106China Guangdong Institute of Intelligence Science and Technology HengqinZhuhaiGuangdong 519031China State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhouZhejiang 310024China School of Life Science and Technology Shanghai Tech UniversityShanghai 200120China

Small cell lung cancer(SCLC)is a recalcitrant cancer featured with high metastasis *** previously found that TAZ acts as a crucial molecular switch in orchestrating SCLC phenotypic transition and metastasis(jin et al.... 详细信息

关键词： metastasis SCLC

Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第2期8卷 730-743页

作者： Zhaoyuan Fang Xiangkun Han Yueqing Chen Xinyuan Tong Yun Xue Shun Yao Shijie Tang Yunjian Pan Yihua Sun Xue Wang yujuan jin Haiquan Chen Liang Hu Lijian Hui Lin Li Luonan Chen Hongbin Ji State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCAS Center for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Zhejiang University-University of Edinburgh Institute Zhejiang University School of MedicineHaining 314400China Department of Thoracic Surgery Fudan University Shanghai Cancer CenterShanghaiChina Department of Oncology Shanghai Medical CollegeFudan UniversityShanghaiChina School of Life Science and Technology ShanghaiTech UniversityShanghai 200120China State Key Laboratory of Molecular Biology Shanghai Institute of Biochemistry and Cell BiologyCAS Center for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310024China

Lkb1 deficiency confers the Kras-mutant lung cancer with strong plasticity and the potential for adeno-to-squamous transdifferentiation(AST).However,it remains largely unknown how Lkb1 deficiency dynamically regulates *** the classical AST mouse model(Kras LSL-G12D/+;Lkb1flox/flox,KL),we here comprehensively analyze the temporal transcriptomic dynamics of lung tumors at different stages by dynamic network biomarker(DNB)and identify the tipping point at which the Wnt signaling is abruptly suppressed by the excessive accumulation of reactive oxygen species(ROS)through its downstream effector *** genetic perturbation of the Wnt pathway using two different Ctnnb1 conditional knockout mouse strains confirms its essential role in the negative regulation of ***,pharmacological activation of the Wnt pathway before but not after the tipping point inhibits squamous transdifferentiation,highlighting the irreversibility of AST after crossing the tipping *** comparative transcriptomic analyses of mouse and human tumors,we find that the lineage-specific transcription factors(TFs)of adenocarcinoma and squamous cell carcinoma form a“Yin-Yang”counteracting ***,inactivation of the Wnt pathway preferentially suppresses the adenomatous lineage TF network and thus disrupts the“Yin-Yang”homeostasis to lean towards the squamous lineage,whereas ectopic expression of NKX2-1,an adenomatous lineage TF,significantly dampens such phenotypic transition accelerated by the Wnt pathway *** negative correlation between the Wnt pathway and AST is further observed in a large cohort of human lung adenosquamous ***,our study identifies the tipping point of AST and highlights an essential role of the ROS-Wnt axis in dynamically orchestrating the homeostasis between adeno-and squamous-specific TF networks at the AST tipping point.

关键词： squamous homeostasis lung

Targeting HSPA1A in ARID2-deficient lung adenocarcinoma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2021年第10期8卷 89-100页

作者： Xue Wang Yuetong Wang Zhaoyuan Fang Hua Wang Jian Zhang Longfu Zhang Hsinyi Huang Zhonglin Jiang yujuan jin Xiangkun Han Shenda Hou Bin Zhou Feilong Meng Luonan Chen Kwok-Kin Wong jinfeng Liu Zhiqi Zhang Xin Zhang Haiquan Chen Yihua Sun Liang Hu Hongbin Ji State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences University of Chinese Academy of Sciences Department of Pulmonary Medicine Zhong Shan Hospital Fudan University Division of Hematology and Medical Oncology Laura and Isaac Perlmutter Cancer Center New York University Langone Medical Center College of Life Sciences Qufu Normal University Shanghai University of Medicine and Health Sciences Shanghai Sixth People's Hospital East Campus Department of General Surgery Shanghai Jiao Tong University Affiliated Sixth People's Hospital Department of Thoracic Surgery Fudan University Shanghai Cancer Center School of Life Science and Technology Shanghai Tech University School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences

Somatic mutations of the chromatin remodeling gene ARID2 are observed in ～7% of human lung adenocarcinomas(LUADs). However, the role of ARID2 in the pathogenesis of LUADs remains largely unknown. Here we find that ARID2 expression is decreased during the malignant progression of both human and mice LUADs. Using two KrasG12 D-based genetically engineered murine models, we demonstrate that ARID2 knockout significantly promotes lung cancer malignant progression and shortens overall survival. Consistently, ARID2 knockdown significantly promotes cell proliferation in human and mice lung cancer cells. Through integrative analyses of Ch IP-Seq and RNA-Seq data, we find that Hspa1 a is up-regulated by Arid2 loss. Knockdown of Hspa1 a specifically inhibits malignant progression of Arid2-deficient but not Arid2-wt lung cancers in both cell lines as well as animal models. Treatment with an HSPA1 A inhibitor could significantly inhibit the malignant progression of lung cancer with ARID2 deficiency. Together, our findings establish ARID2 as an important tumor suppressor in LUADs with novel mechanistic insights, and further identify HSPA1 A as a potential therapeutic target in ARID2-deficient LUADs.

关键词： lung adenocarcinoma tumor suppressor ARID2 HSPA1A

考虑损伤累积效应的矿山边坡稳定性分析及加固方案研究

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

金属矿山 2023年第10期 214-220页

作者：靳玉娟王嘉琪黄河水利职业技术学院土木与交通工程学院河南开封475000

矿山开采过程中,爆破作为一种极其常用的手段,对周围岩体产生了不可逆转的损伤,一旦超过岩体的损伤极限,将会造成边坡失稳,导致严重的安全生产事故。通过声波测试技术,以声波波速为介质定义了损伤变量,用于描述岩石的损伤程度,对矿山爆... 详细信息

矿山开采过程中,爆破作为一种极其常用的手段,对周围岩体产生了不可逆转的损伤,一旦超过岩体的损伤极限,将会造成边坡失稳,导致严重的安全生产事故。通过声波测试技术,以声波波速为介质定义了损伤变量,用于描述岩石的损伤程度,对矿山爆破损伤累积效应进行了现场测试,基于现场实测结果建立了考虑累积效应的损伤模型。将该模型进行二次开发,导入FLAC数值模拟软件,对持续爆破条件下矿山边坡的稳定性进行了分析,并提出了加固方案。研究表明:(1)随着岩体经历爆破次数增加,内部损伤程度逐渐增加,最终以突变激增的形式达到完全破坏;(2)通过拟合试验过程中损伤变量与爆破次数、爆破装药量的关系,认为损伤变量的增长与爆破次数的指数关系成正比,同时与爆破装药量的0.5次方成正比;(3)对频繁爆破条件下的边坡稳定性控制方法进行了分析,所提加固方案有助于控制损伤累积导致的边坡失稳。

关键词：边坡稳定性损伤爆破声波 FLAC

在线全文

学校读者我要写书评

暂无评论

肺癌发病分子机理研究新进展：从基础到临床

中国癌症杂志 2020年第10期30卷 759-769页

作者：金宇娟胡良季红斌中国科学院分子细胞科学卓越创新中心

肺癌在全球范围内具有非常高的发病率和致死率,严重着威胁人类健康。影响肺癌发生、发展及转移的因素十分复杂,可大致分为肿瘤细胞自身因素和肿瘤微环境因素:一方面,肿瘤细胞内基因组、蛋白质组水平的改变,在一定程度上决定了肺癌的特征... 详细信息

肺癌在全球范围内具有非常高的发病率和致死率,严重着威胁人类健康。影响肺癌发生、发展及转移的因素十分复杂,可大致分为肿瘤细胞自身因素和肿瘤微环境因素:一方面,肿瘤细胞内基因组、蛋白质组水平的改变,在一定程度上决定了肺癌的特征;另一方面,肿瘤微环境包括免疫微环境和非免疫微环境,显著地影响肺癌的发生、发展。因此,深入研究肺癌自身及其微环境将有助于加深人们对肺癌发病分子机理的认识,并为改进肺癌的临床诊疗手段奠定重要的理论基础,帮助提高肺癌患者的生活质量及生存时间。对肺癌基础研究的现状进行综述,重点回顾肺癌基因组、蛋白质组、表型可塑性及肺癌微环境领域的相关研究进展,并讨论肺癌临床治疗的新策略。

关键词：肺癌基因组学蛋白质组学肿瘤可塑性肿瘤微环境

端环氧型超支化聚酯对聚(3-羟基丁酸戊酸共聚酯)的改性研究

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国塑料 2016年第10期30卷 42-49页

作者：张静靳玉娟王娥娥北京工商大学材料与机械工程学院北京100048

以2,2-二羟甲基丙酸(DMPA)和三羟甲基丙烷(TMP)为原料,以对甲苯磺酸(P-TSA)为催化剂,通过"一步法"合成三代超支化聚酯(HBPE),然后采用环氧氯丙烷对HBPE进行改性,得到了具有端环氧基的三代超支化聚酯(EHBP-G3)目标产物,最后采用熔融共混... 详细信息

以2,2-二羟甲基丙酸(DMPA)和三羟甲基丙烷(TMP)为原料,以对甲苯磺酸(P-TSA)为催化剂,通过"一步法"合成三代超支化聚酯(HBPE),然后采用环氧氯丙烷对HBPE进行改性,得到了具有端环氧基的三代超支化聚酯(EHBP-G3)目标产物,最后采用熔融共混法制备了EHBP改性的聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV)树脂;通过差示扫描量热仪、偏光显微镜、力学性能和熔体流动速率等分析方法对制备的PHBV/EHBP共混物进行表征。结果表明,加入1.5%(质量分数,下同)三代超支化聚酯(EHBP-G3)时,PHBV/EHBP共混物的结晶度下降了16.1%,冲击强度提高了34.3%。

关键词：超支化聚酯聚(3-羟基丁酸酯-co-3-羟基戊酸酯) 共混改性

在线全文

学校读者我要写书评

暂无评论

青铜文物在动画造型设计中的应用

鞋类工艺与设计 2023年第7期3卷 96-98页

作者：靳玉娟黄河交通学院河南焦作454950

中华民族拥有丰富的艺术设计历史,并诞生了种类多样的艺术精品。其中青铜器起源于夏朝,在商周时期的艺术创造达到顶峰。现存的青铜文物种类丰富,造型多变,显示出先民们优越的设计创造能力和对美好生活的追求。有很多青铜器文物展现出造... 详细信息

中华民族拥有丰富的艺术设计历史,并诞生了种类多样的艺术精品。其中青铜器起源于夏朝,在商周时期的艺术创造达到顶峰。现存的青铜文物种类丰富,造型多变,显示出先民们优越的设计创造能力和对美好生活的追求。有很多青铜器文物展现出造型雄浑,神秘迷人的魅力。在我国的很多动画作品中都有对于青铜器文物的借鉴和再设计。本文从青铜文物的造型与纹饰及工艺角度去做动画造型设计,分析动画造型中可以从青铜器文物中借鉴的内容和方法。将青铜文物融入动画并保留文物的显著特征,一方面让观众了解文物背后的故事与文化,一方面赋予文物灵魂与性格,是宣传传统文化,树立文化自信的有效途径。

关键词：青铜文物动画造型设计文化自信