检索结果-南通市图书馆

Structural basis for stereoselective dehydration and hydrogen-bonding catalysis by the SAM-dependent pericyclase LepI

在线全文

学校读者我要写书评

暂无评论

Structural basis for stereoselective dehydration and hydroge...

Cook宫颈扩张球囊联合硬膜外镇痛在足月引产中的临床研究

第十一届全国化学生物学学术会议

作者： yujuan cai Yang Hai Masao Ohashi Cooper S.Jamieson Marc Garcia-Borras K.N.Houk Jiahai Zhou Yi Tang State Key Laboratory of Bio-organic and Natural Products Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryCAS Department of Chemistry and Biochemistry Department of Chemical and Biomolecular EngineeringUniversity of California Los Angeles

LepI is an S-adenosylmethionine（SAM）-dependent pericyclase that catalyses the formation of 2-pyridone natural product leporin C. Biochemical characterization has shown that LepI can catalyse stereoselective dehydration to yield a reactive（E）-quinone methide that can undergo bifurcating intramolecular Diels–Alder（IMDA） and hetero-Diels–Alder（HDA） cyclizations from an ambimodal transition state, as well as a [3,3]-retro-Claisen rearrangement to recycle the IMDA product into leporin C. Here, we solve the X-ray crystal structures of SAM-bound LepI and in complex with a substrate analogue（the product leporin C） and a retro-Claisen reaction transition-state analogue to understand the structural basis for the multitude of reactions. Structural and mutational analysis reveals how nature evolves a classic methyltransferase active site into one that can serve as a dehydratase and a multifunctional pericyclase. Catalysis of both sets of reactions employs H133 and R295, two activesite residues that are not found in canonical methyltransferases. An alternative role of SAM, which is not found to be in direct contact with the substrate, is also proposed.

关键词： pericyclase crystal structure SAM

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国医药导报 2015年第29期12卷 63-66页

作者：孙丹丹蔡玉娟张望平浙江省嘉兴市妇幼保健院产科浙江嘉兴314000 浙江省嘉兴市妇幼保健院麻醉科浙江嘉兴314000

目的观察Cook宫颈扩张球囊联合硬膜外镇痛在足月引产中的临床效果。方法选择2013年3月-2014年8月嘉兴妇幼保健院足月初产妇80例为研究对象,将其随机分为观察组与对照组,每组40例。观察组在Cook宫颈扩张球囊联合硬膜外镇痛下引产,对照... 详细信息

目的观察Cook宫颈扩张球囊联合硬膜外镇痛在足月引产中的临床效果。方法选择2013年3月-2014年8月嘉兴妇幼保健院足月初产妇80例为研究对象,将其随机分为观察组与对照组,每组40例。观察组在Cook宫颈扩张球囊联合硬膜外镇痛下引产,对照组单纯采用Cook宫颈球囊引产。观察产程时间、产程结局、血流动力学指标、出血量、新生儿Apgar评分及不良反应,采集新生儿脐动脉血血气分析,测定引产前及分娩后例组产妇血常规白细胞计数和超敏C反应蛋白,总体评价产妇分娩舒适度。结果观察组第一产程潜伏期明显短于对照组[（196.60±65.48）比（242.80±78.62）min],差异有高度统计学意义（P〈0.01）,且观察组疼痛减轻;两组产程结局、出血量、新生儿Apgar评分、脐动脉血血气分析、引产术前及分娩后白细胞、超敏C反应蛋白比较,差异无统计学意义（P〉0.05）。两组收缩压、舒张压、心率比较,差异均有统计学意义（P〈0.05或P〈0.01）,注药后0.5、1、2、3、4 h及结束时观察组视觉模拟评分均明显低于对照组（P〈0.05或P〈0.01）。结论 Cook宫颈扩张球囊联合硬膜外镇痛在足月引产中能明显缩短第一产程,减轻产妇疼痛,不增加感染概率,是一种安全有效的方法,可临床推广应用。

关键词：宫颈扩张球囊引产硬膜外镇痛

护患沟通技巧联合心理护理在肺功能检查患者中的应用

在线全文

学校读者我要写书评

暂无评论

齐鲁护理杂志 2024年第3期30卷 21-24页

作者：陈洁张贝磊蔡玉娟陈会敏郑州大学第一附属医院河南郑州450000

目的:探讨护患沟通技巧联合心理护理在肺功能检查患者中的应用效果。方法:选取2020年1月1日~2022年12月1日109例行肺功能检查患者为研究对象,随机分为对照组54例和研究组55例,对照组实施常规护理,研究组实施护患沟通技巧联合心理护理;... 详细信息

目的:探讨护患沟通技巧联合心理护理在肺功能检查患者中的应用效果。方法:选取2020年1月1日~2022年12月1日109例行肺功能检查患者为研究对象,随机分为对照组54例和研究组55例,对照组实施常规护理,研究组实施护患沟通技巧联合心理护理;比较两组检查时间、肺通气完成情况,护理前后心理状态[采用焦虑自评量表(SAS)、抑郁自评量表(SDS)]、肺功能[包括第一秒用力呼气容积(FEV_(1))、FEV_(1)/FVC%]、生活质量[采用生命质量测定量表(QLQ-C30)]及护理满意度。结果:研究组检查时间、肺通气完成率优于对照组(P<0.01);护理后,两组SAS、SDS评分低于护理前(P<0.05),且研究组低于对照组(P<0.01);护理后,两组FEV_(1)、FEV_(1)/FVC%、QLQ-C30评分均优于护理前(P<0.05,P<0.01),且研究组优于对照组(P<0.05);研究组护理满意度高于对照组(P<0.05)。结论:将护患沟通技巧联合心理护理应用于肺功能检查患者中,可有效缩短检查时间,提高肺通气完成率,改善患者负性情绪和肺功能,从而提高生活质量和护理满意度。

关键词：肺功能检查护患沟通技巧心理护理生活质量满意度

任务清单指导下预见性护理对支气管哮喘患儿的影响

在线全文

学校读者我要写书评

暂无评论

齐鲁护理杂志 2023年第11期29卷 5-8页

作者：陈洁张贝磊蔡玉娟陈会敏郑州大学第一附属医院河南郑州450000

目的:探讨任务清单指导下预见性护理对支气管哮喘患儿症状、治疗依从性及焦虑敏感指数的影响。方法:选取2021年1月1日~2023年1月1日收治的143例支气管哮喘患儿作为研究对象,根据不同护理方法分为对照组72例和观察组71例,对照组实施预见... 详细信息

目的:探讨任务清单指导下预见性护理对支气管哮喘患儿症状、治疗依从性及焦虑敏感指数的影响。方法:选取2021年1月1日~2023年1月1日收治的143例支气管哮喘患儿作为研究对象,根据不同护理方法分为对照组72例和观察组71例,对照组实施预见性护理,观察组在对照组基础上实施任务清单指导下预见性护理;比较两组护理各时间段哮喘症状评分、生存质量[采用中文版儿童生存质量哮喘特异性量表(Peds QLTM)]、哮喘控制[采用哮喘控制测试(ACT)]、焦虑敏感性[采用儿童焦虑敏感指数(CASI)]、治疗依从性[采用支气管哮喘用药依从性量表(MARS-A)]。结果:护理后1、2周,两组哮喘症状评分、CASI评分均低于护理前(P<0.05),且观察组低于对照组(P<0.05);护理后,两组Peds QLTM、ACT、MARS-A评分均高于护理前(P<0.05),且观察组高于对照组(P<0.05)。结论:对支气管哮喘患儿进行任务清单指导下预见性护理,可改善患儿哮喘症状,减轻其负性情绪,从而提高治疗依从性。

关键词：任务清单指导预见性护理支气管哮喘治疗依从性生存质量

通乳消痈方联合中医手法排乳治疗哺乳期急性乳腺炎临床研究

在线全文

学校读者我要写书评

暂无评论

新中医 2024年第11期56卷 80-84页

作者：倪小凤周欢周文文蔡玉娟嘉兴市妇幼保健院产科浙江嘉兴314000

目的:观察通乳消痈方联合中医手法排乳治疗哺乳期急性乳腺炎的临床疗效。方法:采用随机数字表法将138例哺乳期急性乳腺炎患者分为中药组、排乳组、联合组各46例。中药组给予通乳消痈方治疗,排乳组给予中医手法排乳治疗,联合组给予通乳... 详细信息

目的:观察通乳消痈方联合中医手法排乳治疗哺乳期急性乳腺炎的临床疗效。方法:采用随机数字表法将138例哺乳期急性乳腺炎患者分为中药组、排乳组、联合组各46例。中药组给予通乳消痈方治疗,排乳组给予中医手法排乳治疗,联合组给予通乳消痈方联合中医手法排乳治疗,3组各治疗5 d。比较3组治疗前后临床症状评分、炎症指标水平;比较3组临床疗效、通乳时间、临床症状消除时间、体温恢复正常时间及不良反应发生率。结果:联合组总有效率97.83%,高于中药组82.61%、排乳组86.96%(P0.05)。治疗后,3组临床症状评分、白细胞计数(WBC)、白细胞介素-6(IL-6)、C-反应蛋白(CRP)水平均较治疗前降低(P0.05)。3组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:通乳消痈方联合中医手法排乳治疗哺乳期急性乳腺炎效果良好,能够减轻患者临床症状,降低炎症因子水平,安全性较高。

关键词：急性乳腺炎哺乳期通乳消痈方中医手法排乳炎症因子

Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng Li Meifeng cai Shengjun Miao Yuan Li Yu Wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

“袋鼠爸爸式护理”对剖宫产术后新生儿生长发育的影响

在线全文

学校读者我要写书评

暂无评论

护理与康复 2019年第3期18卷 43-46页

作者：沈红丽蔡玉娟任玮孙丽平嘉兴市妇幼保健院浙江嘉兴314000

目的评估"袋鼠爸爸式护理"对剖宫产新生儿生长发育、生命体征等的影响。方法将120例剖宫产新生儿按随机数字法分为观察组("袋鼠爸爸式护理")60例与对照组(常规护理)60例。评估两组新生儿的神经行为功能、身体发育(头围、体质量、身高的... 详细信息

目的评估"袋鼠爸爸式护理"对剖宫产新生儿生长发育、生命体征等的影响。方法将120例剖宫产新生儿按随机数字法分为观察组("袋鼠爸爸式护理")60例与对照组(常规护理)60例。评估两组新生儿的神经行为功能、身体发育(头围、体质量、身高的增长)、生命体征(心率、呼吸、血氧饱和度)、情感需求及总胆红素。结果与护理前相比,两组新生儿的神经行为功能、身体发育、生命体征、情感需求均得到显著的改善(P均<0.05);经过护理后,观察组神经行为功能、身体发育、生命体征、情感需求的改善明显优于对照组(P均<0.05)。结论对剖宫产新生儿采用"袋鼠爸爸式护理"能促进其生长发育。

关键词：新生儿袋鼠式护理神经行为生命体征

SensFL:Privacy-Preserving Vertical Federated Learning with Sensitive Regularization

在线全文

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2025年第1期142卷 385-404页

作者： Chongzhen Zhang Zhichen Liu Xiangrui Xu Fuqiang Hu Jiao Dai Baigen cai Wei Wang School of Automation and Intelligence Beijing Jiaotong UniversityBeijing100044China Shuohuang Railway Development Co. Ltd.National Energy GroupCangzhou062350China Beijing Key Laboratory of Security and Privacy in Intelligent Transportation School of Computer Science and TechnologyBeijing Jiaotong UniversityBeijing100044China

In the realm of Intelligent Railway Transportation Systems,effective multi-party collaboration is crucial due to concerns over privacy and data *** Federated Learning(VFL)has emerged as a promising approach to facilitate such collaboration,allowing diverse entities to collectively enhance machine learning models without the need to share sensitive training ***,existing works have highlighted VFL’s susceptibility to privacy inference attacks,where an honest but curious server could potentially reconstruct a client’s raw data from embeddings uploaded by the *** vulnerability poses a significant threat to VFL-based intelligent railway transportation *** this paper,we introduce SensFL,a novel privacy-enhancing method to against privacy inference attacks in ***,SensFL integrates regularization of the sensitivity of embeddings to the original data into the model training process,effectively limiting the information contained in shared *** reducing the sensitivity of embeddings to the original data,SensFL can effectively resist reverse privacy attacks and prevent the reconstruction of the original data from the *** experiments were conducted on four distinct datasets and three different models to demonstrate the efficacy of *** results show that SensFL can effectively mitigate privacy inference attacks while maintaining the accuracy of the primary learning *** results underscore SensFL’s potential to advance privacy protection technologies within VFL-based intelligent railway systems,addressing critical security concerns in collaborative learning environments.

关键词： Vertical federated learning privacy defenses

Identifying the dynamic behaviors in complete reconstruction of Co-based complex precatalysts during electrocatalytic oxygen evolution

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 226-233页

作者： Jingfang Zhang Danyang Wu Linke cai Youluan Lu Fanpeng Cheng Lijuan Shi Qun Yi Yao Liu Yi Huang Key Laboratory of Green Chemical Engineering Process of Ministry of Education Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education Hubei Key Laboratory of Novel Reactor and Green Chemical Technology School of Chemical Engineering & Pharmacy Wuhan Institute of Technology Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction of Ministry of Education College of Chemistry Central China Normal University College of Materials Science and Engineering Fuzhou University

Transition metal-based nanomaterials have emerged as promising electrocatalysts for oxygen evolution reaction（OER）. Considerable research efforts have shown that self-reconstruction occurs on these nanomaterials under operating conditions of OER process. However, most of them undergo incomplete reconstruction with limited thickness of reconstruction layer, leading to low component utilization and arduous exploration of real catalytic mechanism. Herein, we identify the dynamic behaviors in complete reconstruction of Co-based complexes during OER. The hollow phytic acid（PA） cross-linked CoFe-based complex nanoboxes with porous nanowalls are designed because of their good electrolyte penetration and mass transport ability, in favor of the fast and complete reconstruction. A series of experiment characterizations demonstrate that the reconstruction process includes the fast substitution of PA by OH-to form Co（Fe）(OH)xand subsequent potential-driven oxidation to Co（Fe）OOH. The obtained CoFeOOH delivers a low overpotential of 290 mV at a current density of 10 mA cm-2and a long-term *** experiment results together with theory calculations reveal that the Fe incorporation can result in the electron rearrangement of reconstructed CoFeOOH and optimization of their electronic structure,accounting for the enhanced OER activity. The work provides new insights into complete reconstruction of metal-based complexes during OER and offers guidelines for rational design of high-performance electrocatalysts.

关键词： Complete reconstruction Co-based complex Precatalysts Oxygen evolution reaction