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The Detection of SARS-CoV with SPR Biosensor

稀有金属材料与工程 2006年第A3期35卷 288-290页

作者： yujie wang {2. cui junbo wang haoyuan cai zipan zheng wei deng {12. qin xiuhong yang State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Science Beijing 100080 China The Institute of Laboratory Animal Science CAMS ＆ PUMC Beijing 100021 China

SPR (Surface Plasmon Resonance) is an emerging optical biosensor which can monitor the processes at metal interface in real time without labeling *** SPR-2.04 can sense two areas in one channel and get two signals at same time in one *** signal is the graph of the reaction;the other is the graph of *** biochip is modified with dextran,and only reaction area can be used to immobilize the *** methods of antibody immobilization on chip were *** was to immobilize directly,the other was to immobilize protein A firstly and then the antibody was caught by immobilized protein *** latter was chosen as suitable for retention of the native binding ability with virus of *** latter chip was used to detect *** signal of detection reached to 60 units within 40 *** was 55 units even after reference.

关键词： SPR-2.04 biosensor SARS-CoV detection antibody

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