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Numerical simulation and experimental study on eggshell membrane separation device

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International Journal of Agricultural and Biological Engineering 2019年第2期12卷 173-183页

作者： Yuan chi Yong Wang Mengfu Li Jie Ren yujie chi College of Engineering Northeast Agricultural UniversityHarbin 150030China College of Food Science Northeast Agricultural UniversityHarbin 150030China

In order to provide theoretical guidance for separating egg membrane from eggshell by using mechanical agitation,CFD was used to explore the flow characteristics in stirred tank,using the Sliding Grid method to deal with the impeller rotational velocity zone in flow field,and using the Euler model to deal with liquid-solid two-phase *** study explored the influence of dish-shape bottom or flat-shape bottom,the clearance size between baffle and the side wall,and the axial height of impeller to bottom on suspension state of particles,solids holdup distribution,solid phase velocity and power number by *** results showed that better particles suspension effect in dish-shape tank can reduce particles accumulation at the bottom and power *** there was a small clearance size(S)between the baffle and the side wall of the stirred tank,it would reduce particles accumulation at the bottom,and reduce the power ***,too large S would decrease the suspension height of particles,not only cannot strengthen the main flow,but also lead to most fluid through clearance forming tangential flow,simulation results showed that S=6 mm was *** decreased axial height of impeller(C)to bottom,particles accumulation at the bottom was decreased,but power consumption would increase,simulation results showed that C=H/5(H is height of liquid surface)was *** to the simulation results,the structure of the stirring tank was *** the same time,the influences of stirring rotational velocity,stirring time,solid-liquid ratio and separating medium temperature on egg membrane recovery were also studied by experiment,and optimal parameter combination of factors was *** experiment results showed while the stirring time was 17.1 min,stirring rotational velocity was 350 r/min,solid-liquid ratio was 1:17 g/mL,the separating medium temperature was 32℃,the membrane recovery rate can reach above 89%.The device improves the recovery a

关键词： liquid-solid two-phase flow mechanical stirring field characteristics eggshell membrane separation

Differences in protein composition and functional properties of egg whites from four chicken varieties

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Food Bioscience 2022年第2期46卷 934-941页

作者： Ruihan Yu Yuan chi Yanqiu Ma yujie chi Lifeng Wang College of Food Science Northeast Agricultural UniversityHarbinHeilongjiang150030China College of Engineering Northeast Agricultural UniversityHarbinHeilongjiang150030China

Egg white protein(EWP)is widely used in the food industry for its technological properties,which are affected by diet-and breed-related changes in its ***,the objective of this study was to analyse the relationship between hen variety and the protein composition,functional characteristics,and physicochemical properties of egg white(EW)obtained from Black Bone(BB),Gushi(GS),Hy-Line(HY-LINE),and Harbin White(HW)*** protein composition of EW was analysed by high performance liquid chromatography(HPLC),while the secondary structure of EWP was assessed by Raman *** and GS EWs had significantly higher ovalbumin content(183.38 and 168.56μg/mL,respectfully)than HY-LINE and HW EWs(148.15 and 147.44μg/mL,respectfully)(pchildren’s growth and preterm infant *** conclusion,differences in protein composition likely led to dissimilar functional and physicochemical properties among EWs obtained from different hen varieties,which could provide a reference for food production and genetic breeding of laying hens.

关键词： Egg white protein Protein content Functional and physicochemical properties High performance liquid chromatography

Expression and analysis of zinc fi nger family gene in Lenzites gibbosa

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Journal of Forestry Research 2020年第5期31卷 1889-1898页

作者： Jun Zhang yujie chi Shuxuan Li Jian Zhang Jie Chen School of Forestry Northeast Forestry UniversityHarbin 150040People’s Republic of China Forestry Research Institute Xinjiang Agricultural UniversityUrumqi 830052People’s Republic of China

Zinc finger transcription factors play significant roles in the growth and development of plant and animal,but their function remains obscure in *** gibbosa mycelia were extracted and sequenced by transcriptome analysis after growing on sawdust at different times to support mycelial growth of *** in a nutrient *** bases used for analysis were the Kyoto encyclopedia of genes and genomes(KEGG)annotation,the cluster of orthologous groups of proteins(COG)and gene ontology(GO)*** finger class genes related to the growth and development of *** were *** annotation and enrichment analysis of diff erentially expressed genes were carried out.A total of 114.55 Gb Clean Data were obtained from the *** *** average Clean Data in each sample was 6.16 *** relative efficiency of reads between each sample and the reference genome was 88.5%to 91.4%.The COG analysis showed that most zinc finger protein genes were related to replication,recombination and repair *** enrichment analysis showed that the expressed genes involved in cellular process,cell part and *** identifi ed seventy-two expressed genes including seven up-regulated genes and sixty-five down-regulated genes by applying DESeq2 data analysis *** comparing the significantly expressed genes with KEGG database,66 annotated sequences were obtained,and 35 primary metabolic pathways were *** enrichment analysis showed that differentially expressed genes were signifi cantly enriched in protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis ***_11750 and gene_5266 are highly correlated with the growth and development of *** and are closely related to protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis *** to gene functional analysis,seven important differentially expressed genes related to the growth and development of *** were identified

关键词： Lenzites gibbosa Differently expressed genes The transcriptome Zinc finger

Baeyer-Villiger Oxidation of Cyclic Ketones Catalyzed by Amino Acid Ionic Liquids

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Chemical Research in chinese Universities 2020年第5期36卷 865-869页

作者： YU Fengli chi yujie GAO Chong CHEN Ruirui XIE Congxia YU Shitao State Key Laboratory Base of Eco-chemical Engineehng College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao 266042P.R.China College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042P.R.China

A series of amino-acid-based ionic liquids was synthesized via one-step protonation of the corresponding L-amiiio acid by utilizing an array of proton *** catalytic activity of the amino-acid-based ionic liquids for the Baeyer-Villiger oxidation of cyclic ketones was investigated using cyclopentanone as a model ***-based ionic liquid[ProH]CF3SO3 was shown to exliibit thebest catalytic *** reaction condition was optimized to give the following reagent ratio of h(cyclopentanone):n(catalyst):w(H2O2)=1:0.06:4,60℃and 6 *** the optimum conditions,the conversion of cyclopentanone was 96.57%and the selectivity forδ-valerolactone was 73.01%.The catalytic activity was shown to be constant after 4 cycles.A simple treatment was allowed for the recover and the reuse of[ProH]*** successful utilization of[ProH]CF3SO3 to catalyze a host of cyclic ketones via Baeyer-Villiger oxidation clearly demonstrated the capacity of[ProH]CF3S03 to tolerate variation in the substrate.

关键词： Amino acid ionic liquid Baeyer-Villiger oxidation Cyclic ketone Lactone

野菊复合体生态适宜性分析及未来潜在产地布局预测

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世界科学技术-中医药现代化 2025年第1期27卷 75-83页

作者：张海琼朱明玉池玉洁刘义飞胡志刚张景景湖北中医药大学药学院湖北时珍实验室

目的分析未来时期野菊复合体物种的潜在生态适宜产区，以提供科学依据进行布局规划和规范化种植。方法通过收集全国范围内1379个有效的野菊复合体物种分布数据和104个生态因子，结合MaxEnt模型和ArcGIS软件，对17种不同气候情景下野... 详细信息

目的分析未来时期野菊复合体物种的潜在生态适宜产区，以提供科学依据进行布局规划和规范化种植。方法通过收集全国范围内1379个有效的野菊复合体物种分布数据和104个生态因子，结合MaxEnt模型和ArcGIS软件，对17种不同气候情景下野菊复合体的潜在生态分布区进行分析。结果降雨量和太阳辐射是影响野菊复合体适宜生境的关键环境变量。野菊复合体最适宜生长的区域分别集中在湖北（野菊）、山西（甘菊）、江苏（菊花脑）和湖北（神农香菊）地区。在不同情景下，野菊和甘菊的适生区面积整体呈缩减趋势，神农香菊和菊花脑的适生区面积则呈扩张趋势。质心迁移表明了野菊复合体对气候变化表现出敏感性。结论对野菊复合体的潜在分布区分别进行了生态适宜性等级划分，为资源的可持续利用、引种栽培以及科学区划提供了重要的参考依据。

关键词：野菊复合体神农香菊 MaxEnt ArcGIS 生态适宜性

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Variation of Serum Protein Level in Xiang Piglets at Different Month of Age

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Journal of Northeast Agricultural University(English Edition) 1995年第2期2卷 118-122页

作者： chi yujie, Gao Xuejun, Yan Libo(Northeast Agricultural University, Harbin 150030, P R C)

Usual clinieal methods were used to determine the concentrations of scrum protein, albumin and globulin in Xiang piglets from the 1st month to the 6th month of age. Thirteen piglcts(cight males and five females) born in Spring were selected from the Livestock Experimental Station of NEAU. The results showed that the scrum protein level was different at different month of age. The lowest level was found at the 1st month of age, and the highest at the 4th month of age. The difference in scrum protein concentration was significant between different months of age. There was, however, no significant difference between sexes at the same month of age.

关键词： xiang piglet blood protein variation

基于Box-Behnken模型的卵清蛋白糖基化制备技术

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农业机械学报 2009年第11期40卷 138-143页

作者：于滨迟玉杰东北农业大学食品学院哈尔滨150030

采用Box-Behnken模型对卵清蛋白糖基化反应过程进行优化,测定并分析了糖基化卵清蛋白在各种条件下的乳化性质。结果表明其最佳反应条件为:糖添加量9%,反应时间48 h,反应温度60℃,在此条件下乳化活性为1.328,乳化稳定性为9.63 min,相对... 详细信息

采用Box-Behnken模型对卵清蛋白糖基化反应过程进行优化,测定并分析了糖基化卵清蛋白在各种条件下的乳化性质。结果表明其最佳反应条件为:糖添加量9%,反应时间48 h,反应温度60℃,在此条件下乳化活性为1.328,乳化稳定性为9.63 min,相对卵清蛋白分别提高了3.7倍和8.75倍。糖基化程度与乳化活性及乳化稳定性的关系符合Lerentz函数。糖基化卵清蛋白的乳化性质相对不受低pH值和高离子强度的影响,且优于商用乳化剂。研究结果证明,糖基化是提高卵清蛋白乳化性质的一种有效途径。

关键词：卵清蛋白糖基化乳化性质 Box-Behnken模型

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高凝胶性大豆球蛋白制备工艺优化

农业机械学报 2012年第10期43卷 124-130页

作者：迟玉杰范淼东北农业大学教育部大豆生物学重点实验室哈尔滨150030

以脱脂豆粕为材料,分离纯化后得到大豆球蛋白(11S)。通过添加葡萄糖进行糖基化改性,单因素试验初步得到改性的工艺条件,并在此基础上采用Box-Behnken模型对工艺条件进行优化,测定并分析了改性产物在各个条件下的凝胶强度。结果表明,最... 详细信息

以脱脂豆粕为材料,分离纯化后得到大豆球蛋白(11S)。通过添加葡萄糖进行糖基化改性,单因素试验初步得到改性的工艺条件,并在此基础上采用Box-Behnken模型对工艺条件进行优化,测定并分析了改性产物在各个条件下的凝胶强度。结果表明,最佳工艺条件为:反应温度78.61℃、反应时间60.36 min和葡萄糖质量分数2.84%,凝胶强度达到271.37 g。根据实际情况,将反应温度设定为80℃,反应时间设定为60 min,葡萄糖质量分数确定为3.0%,在此条件下进行验证试验,凝胶强度为270.52 g,是未改性大豆球蛋白的3.07倍。试验证明优化工艺能有效且显著提高大豆球蛋白的凝胶强度。

关键词：大豆球蛋白糖基化改性凝胶强度 Box-Behnken模型

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挤压豆渣中可溶性膳食纤维制备工艺的优化

农业工程学报 2009年第6期25卷 285-289页

作者：娄海伟迟玉杰东北农业大学食品学院哈尔滨150030 大豆生物学省部共建教育部重点实验室哈尔滨150030

为了获得碱法制备挤压豆渣中可溶性膳食纤维的最适工艺参数,以液固比、温度、时间和碱浓度为试验因子,以可溶性膳食纤维产率为响应值,采用中心旋转组合试验设计进行试验。结果表明:4个因素对可溶性膳食纤维产率的影响大小依次为碱浓度>... 详细信息

为了获得碱法制备挤压豆渣中可溶性膳食纤维的最适工艺参数,以液固比、温度、时间和碱浓度为试验因子,以可溶性膳食纤维产率为响应值,采用中心旋转组合试验设计进行试验。结果表明:4个因素对可溶性膳食纤维产率的影响大小依次为碱浓度>液固比>温度>时间。通过典型性分析得出可溶性膳食纤维最适制备条件为:液固比26∶1、温度89℃、时间68min、碱浓度1.12%。在此条件下,可溶性膳食纤维产率的预测值为33.96%,验证试验所得可溶性膳食纤维产率为34.12%。回归方程的预测值和试验值差异不显著,所得回归模型拟合情况良好,达到设计要求。在本试验优化的条件下,以经挤压豆渣为原料制备的可溶性膳食纤维产率(34.12%)显著高于以未经挤压豆渣为原料的产率(13.51%)。

关键词：可溶性膳食纤维工艺优化挤压豆渣