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The effect of collagen hydrogels on chondrocyte behaviors through restricting the contraction of cell/hydrogel constructs

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Regenerative Biomaterials 2021年第4期8卷 62-71页

作者： Longpeng Dong Qingli Liu Yongli Gao Hengxing Jia Wenling Dai Likun Guo Hongsong fan yujiang fan Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan University29 Wangjiang RoadChengduSichuan 610064PR China

Collagen is a promising material for tissue engineering,but the poor mechanical properties of collagen hydrogels,which tend to cause contraction under the action of cellular activity,make its application *** this study,the amino group of type I collagen(Col I)was modified with methacrylic anhydride(MA)and the photo-crosslinkable methacrylate anhydride modified type I collagen(CM)with three different degrees of substitution(DS)was *** physical properties of CM and Col I hydrogels were tested,including micromorphology,mechanical properties and degradation *** results showed that the storage modulus and degradation rate of hydrogels could be adjusted by changing the DS of *** vitro,chondrocytes were seeded into these four groups of hydrogels and subjected to fluorescein diacetate/propidium iodide(FDA/PI)staining,cell counting kit-8(CCK-8)test,histological staining and cartilage-related gene expression *** vivo,these hydrogels encapsulating chondrocytes were implanted subcutaneously into nude mice,then histological staining and sulfated glycosaminoglycan(sGAG)/DNA assays were *** results demonstrated that contraction of hydrogels affected behaviors of chondrocytes,and CM hydrogels with suitable DS could resist contraction of hydrogels and promote the secretion of cartilage-specific matrix in vitro and in vivo.

关键词： type I collagen contraction photo-crosslinkable hydrogel chondrocyte

Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo

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Regenerative Biomaterials 2020年第6期7卷 583-595页

作者： Xiuyu Wang Yan Lu Wan Wang Qiguang Wang Jie Liang yujiang fan Xingdong Zhang Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration Guangxi Collaborative Innovation Center for BiomedicineGuangxi Medical UniversityNanning 530021China National Engineering Research Center for Biomaterials Sichuan UniversityWangjiang Road 29Chengdu 610064China

Extracellular matrix(ECM)-based biomaterials are promising candidates in cartilage tissue engineering by simulating the native microenvironment to regulate the chondrogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)without exogenous growth *** biological properties of ECM scaffolds are primarily depended on the original source,which would directly influence the chondrogenic effects of the ECM *** the expanding investigations on ECM scaffolds in recent years,the selection of optimized ECM materials in cartilage regeneration was less *** this study,we harvested and compared the articular cartilage ECM from newborn,juvenile and adult *** results demonstrated the significant differences in the mechanical strength,sulphated glycosaminoglycan and collagen contents of the different aged ECM,before and after ***,different compositional and mechanical properties were shown in the three ECM-based collagen hydrogels,which exerted age-dependent chondrogenic *** general,both in vitro and in vivo results suggested that the newborn ECM promoted the most chondrogenesis of BMSCs but led to severe matrix *** contrast,BMSCs synthesized the lowest amount of cartilaginous matrix with minimal calcification with adult *** juvenile ECM achieved the best overall results in promoting chondrogenesis of BMSCs and preventing matrix ***,this study provides important information to our current knowledge in the design of future ECM-based biomaterials towards a successful repair of articular cartilage.

关键词： cartilage ECM mesenchymal stem cells collagen hydrogel chondrogenesis calcification

Oral health in China: from vision to action

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International Journal of Oral Science 2018年第1期10卷 1-6页

作者： Xuedong Zhou Xin Xu Jiyao Li Deyu Hu Tao Hu Wei Yin yujiang fan Xingdong Zhang State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of StomatologySichuan University State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Preventive Dentistry West China Hospital of StomatologySichuan University National Engineering Research Center for Biomaterials Sichuan University

Chinese president Xi Jinping made clear at the National Health and Wellness Conference that health is the prerequisite for people’s all-around development and a precondition for the sustainable development of China. Oral health is an indispensable component of overall health in humans. However, the long neglect of oral health in overall health agendas has made oral diseases an increasing concern. With this perspective, we described the global challenges of oral diseases, with an emphasis on the challenges faced by China. We also described and analyzed the recently released health policies of the Chinese government, which aim to guide midterm and long-term oral health promotion in China. More importantly, we called for specific actions to fulfill the larger goal of oral health for the nation. The implementation of primordial prevention efforts against oral diseases, the integration of oral health into the promotion of overall health, and the management of oral diseases in conjunction with other chronic non-communicable diseases with shared risk factors were highly recommended. In addition, we suggested the reform of standard clinical residency training, the development of domestic manufacturing of dental equipment and materials, the revitalization traditional Chinese medicine for the prevention and treatment of oral diseases, and integration of oral health promotion into the Belt and Road Initiative. We look forward to seeing a joint effort from all aspects of the society to fulfill the goal of Healthy China 2030 and ensure the oral health of the nation.

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3D printing of calcium phosphate bioceramic with tailored biodegradation rate for skull bone tissue reconstruction

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Bio-Design and Manufacturing 2019年第3期2卷 161-171页

作者： Boqing Zhang Huan Sun Lina Wu Liang Ma Fei Xing Qingquan Kong yujiang fan Changchun Zhou Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China Department of Orthopedics West China HospitalSichuan UniversityChengdu 610041China State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310058China

The bone regenerative scaffold with the tailored degradation rate matching with the growth rate of the new bone is essential for adolescent bone *** satisfy these requirement,we proposed bone tissue scaffolds with controlled degradation rate using osteoinductive materials(Ca-P bioceramics),which is expected to present a controllable biodegradation rate for patients who need bone *** properties,porosity,compressive strength and degradation properties of the scaffolds were studied.3D printed Ca-P scaffold(3DS),gas foaming Ca-P scaffold(FS)and autogenous bone(AB)were used in vivo for personalized beagle skull defect *** results indicated that the 3DS was highly vascularized and well combined with surrounding *** showed obvious newly formed bone *** showed the best repair effect,but it was found that AB scaffolds were partially absorbed and *** study indicated that the 3D printed Ca-P bioceramics with tailored biodegradation rate is a promising candidate for personalized skull bone tissue reconstruction.

关键词： 3D printing Skull repair Calcium phosphate ceramics Tailored biodegradation rate Bone reconstruction

The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic

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Regenerative Biomaterials 2018年第3期5卷 129-139页

作者： Ying Chen Jing Wang Xiangdong Zhu Xuening Chen Xiao Yang Kai Zhang yujiang fan Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China

Osteoinductivity of porous calcium phosphate(CaP)ceramics has been widely investigated and confirmed,and it might be attributed to the rapid formation of the vascular networks after in vivo implantation of the *** this study,to explore the vascularization mechanism within the CaP ceramics,the migration and differentiation of bone marrow-derived mesenchymal stem cells(BMSCs)under the stimulation of porous biphasic calcium phosphate(BCP)ceramic with excellent osteoinductivity were systematically *** results indicated that the directional migration of BMSCs toward BCP ceramic occurred when evaluated by using a transwell model,and the BMSCs migration was enhanced by the seeded macrophages on the ceramic in ***,by directly culturing BMSCs on BCP ceramic discs under both in vitro and in vivo physiological environment,it was found that the differentiation of BMSCs toward vascular endothelial cells(VECs)happened under the stimulation of BCP ceramic,as was confirmed by the up-regulated gene expressions and protein secretions of VECs-related characteristic factors,including kinase insert domain receptor,von willebrand factor,vascular cell adhesion molecule-1 and cadherin 5 in the *** study offered a possibility for explaining the origin of VECs during the rapid vascularization process after in vivo implantation of porous CaP ceramics and could give some useful guidance to reveal the vascularization mechanism of the ceramics.

关键词： BCP ceramic vascularization BMSCs VECs migration differentiation

Therapeutic sponge prevents postoperative breast cancer recurrence by sustainably dissociating into CD44-targeted nanoplatform

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Nano Research 2024年第3期17卷 1792-1803页

作者： Junhui Sui Mingda Zhao Zhihao Guo Jiafeng Li Jie Chen Hongli Chen Jie Liang Yong Sun Xingdong Zhang yujiang fan National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China Beijing Tide Pharmaceutical Co. Ltd.Beijing 100176China The Key Laboratory of Biomedical Material School of Life Science and TechnologyXinxiang Medical UniversityXinxiang 453003China Coal Mining Branch China Coal Research InstituteBeijing 100028China School of Ophthalmology&Optometry Eye HospitalWenzhou Medical UniversityWenzhou 325027China

Locoregional recurrence and distant metastasis of breast cancer still pose a significant risk for patients’*** address the clinical challenge,functional absorbable sponges(HA-SH/PP-Dox/Lap/COL I(HCNPs))were constructed by biomimetic extracellular matrix of collagen I/hyaluronic acid complex conjugated with doxorubicin/lapatinib(Dox/Lap)-loaded *** HCNPs sponge exhibited excellent clotting ability and blood absorption ***,Dox/Lap-loaded nanoparticles were synchronously endowed with a large number of oligo hyaluronic acid segments after degradation,which thus enhanced the ability of targeting into CD44-overexpressed tumor *** implantable HCNPs sponge in resected cavity of postoperative 4T1 models inhibited the spread of scattered tumor cells by absorbing the inevitable *** importantly,CD44 targeted nanoparticle with suitable Dox/Lap proportion continuously released from sponge to kill tumor cells of surrounding HCNPs and those remaining at surgical margin,thus prevented local recurrence as well as distant ***,the functional HCNPs sponge might provide a safer and more effective strategy for postoperative treatment of cancer.

关键词： therapeutic sponge scattered tumor cells recurrence and metastasis CD44-targeted nanoparticles breast cancer

A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model

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Regenerative Biomaterials 2021年第1期8卷 52-62页

作者： Wen Zou Xing Li Na Li Tianwei Guo Yongfu Cai Xiaoqin Yang Jie Liang Yong Sun yujiang fan National Engineeing Research Center for Biomaterials Sichuan University29 Wangiang RoadChengdu 610064SichuanChina Sichuan Testing Centre for Biomaterials and Medical Devices 29 Wangjiang RoadChengdu 610064SichuanChina

Repair and reconstruction of large bone defect were often difficult,and bone substitute materials,including autogenous bone,allogenic bone and artificial bone,were common treatment *** key to elucidate the clinical effect of these bone repair materials was to study their osteogenic capacity and immunotoxicological *** this paper,the mechanical properties,micro-CT imaging analysis,digital image analysis and histological slice analysis of the three bone grafts were investigated and compared after different time points of implantation in rat femur defect *** bone and biphasic calcium phosphate particular artificial bone containing 61.4% HA and 38.6%β-tricalcium phosphate with 61.64%porosity and 0.8617±0.0068 g/cm^(3) den-sity(d≤2 mm)had similar and strong bone repair ability,but autogenous bone implant materials caused greater secondary damage to experimental animals;allogenic bone exhibited poor bone defect repair *** the early stage of implantation,the immunological indexes such as Immunoglobulin G,Immunoglobulin M concentration and CD4 cells'population of allogenic bone significantly increased in compared with those of autologous bone and artificial *** the repair process of artificial bone was relatively inefficient than autologous bone graft,the low immunotoxicological indexes and acceptable therapeutic effects endowed it as an excellent alter-native material to solve the problems with insufficient source and secondary trauma of autogenous bone.

关键词： autogenous bone alloge nic bone artificial bone bone defect repair osteogenesis immunotoxicity

BMSCs-assisted injectable Col I hydrogel-regenerated cartilage defect by reconstructing superficial and calcified cartilage

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Regenerative Biomaterials 2020年第1期7卷 35-45页

作者： Hanxu Cai PeileiWang Yang Xu Ya Yao Jia Liu Tao Li Yong Sun Jie Liang yujiang fan Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan University29 Wangjiang RoadChengdu 610064P.R.China Department of Gynecology and Obstetrics Development and Related Disease of Women and Children Key Laboratory of Sichuan ProvinceKey Laboratory of Birth Defects and Related Diseases of Women and ChildrenMinistry of EducationWest China Second HospitalSichuan University20 Renmin South RoadChengdu 610041P.R.China

The self-healing capacity of cartilage was limited due to absence of vascular,nervous and lymphatic *** many clinical treatments have been used in cartilage defect repair and shown a promising repair result in short term,however,regeneration of complete zonal structure with physiological function,reconstruction cartilage homeostasis and maintaining long-term repair was still an unbridgeable *** has complex zonal structure and multiple physiological functions,especially,superficial and calcified cartilage played an important role in keeping *** address this hurdle of regenerating superficial and calcified cartilage,injectable tissue-induced type I collagen(Col I)hydrogel-encapsulated BMSCs was chosen to repair cartilage *** 1 month implantation,the results demonstrated that Col I gel was able to induce BMSCs differentiation into chondrocytes,and formed hyaline-like cartilage and the superficial layer with lubrication *** 3 months post-surgery,chondrocytes at the bottom of the cartilage layer would undergo hypertrophy and promote the regeneration of calcified *** months later,a continuous anatomical tidemark and complete calcified interface were *** regeneration of neo-hyaline cartilage was similar with adjacent normal tissue on the thickness of the cartilage,matrix secretion,collagen type and *** multilayer zonal structure with physiological function remodeling indicated that BMSCs-assisted injectable Col I hydrogel could reconstruct cartilage homeostasis and maintain long-term therapeutic effect.

关键词： BMSCs injectable Col I hydrogel superficial cartilage,calcified cartilage

Biofabrication (3D Bioprinting) Laboratory at Sichuan University

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Bio-Design and Manufacturing 2021年第2期4卷 432-439页

作者： Changchun Zhou Kefeng Wang Yong Sun Qiguang Wang Qing Jiang Jie Liang Xuan Pei Boqing Zhang yujiang fan Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China College of Biomedical Engineering Sichuan UniversityChengdu 610064China College of Materials Science and Engineering Sichuan UniversityChengdu 610064China

Introduction and research overview Recently,increasing need for organ transplantation and lack of donated organs have led to the rapid development of new technologies for artificial organ *** the era of burgeoning breakthroughs around 3D bioprinting technologies,the personalization of organs and medicine is an ongoing nice vision[1–5].As one of the leading laboratories in the interdisciplinary field of materials,manufacturing and bioengineering,the Biofabrication(3D Bioprinting)Research Laboratory at Sichuan University has been engaging in the research on customized regenerativemedicine since 2012.

关键词： printing organs Laboratory