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Single-cell analysis defines the lineage plasticity of stem cells in cervix epithelium

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Cell Regeneration 2021年第1期10卷 367-384页

作者： Zixian Zhao yujia wang Yingchuan Wu Dandan Li Ting Zhang Yu Ma Xiaoming Teng Wei Zuo East Hospital School of MedicineTongji UniversityShanghaiChina Super Organ R&D Center Regend TherapeuticsShanghaiChina Shanghai Jincai East Secondary School ShanghaiChina Shanghai First Maternity and Infant Hospital Tongji UniversityShanghaiChina Ningxia Medical University YinchuanChina The First Affiliated Hospital Guangzhou Medical UniversityGuangzhouChina

Information about the dynamic change and post-injury regeneration of cervical epithelium is relatively rare,even though it is tightly related to gynecologic ***,using a feeder cell-based culturing system,we stably cloned mouse and human P63 and KRT5 expressing cells from the adult cervix as putative cervical stem/progenitor cells(CVSCs).When subjected to differentiation,the cultured cells gave rise to mature cervical epithelium by differentiating into squamous or glandular *** ability of endogenous mouse CVSCs to reconstitute cervical epithelium after injury was also evident from the genetic lineage tracing ***-cell transcriptomic analysis further classified the CVSCs into three subtypes and delineated their bi-lineage differentiation roadmap by pseudo-time *** also tracked the real-time differentiation routes of two representing single CVSC lines in vitro and found that they recapitulated the predicted roadmap in pseudo-time *** pathways including Wnt,TGF-beta,Notch and EGFR were found to regulate the cervical epithelial hierarchy and implicated the different roles of distinct types of cells in tissue homeostasis and ***,the above data provide a cloning system to achieve stable in vitro culture of a bi-lineage stem/progenitor cell population in the cervix,which has profound implications for our understanding of the cervix stem/progenitor cell function in homeostasis,regeneration,and disease and could be helpful for developing stem cell-based therapies in future.

关键词： Cervix Stem cells Regeneration Single-cell RNA-seq

通智测试——基于动态具身物理社会交互环境的通用人工智能测试

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Engineering 2024年第3期34卷 12-22页

作者： yujia Peng Jiaheng Han Zhenliang Zhang Lifeng Fan Tengyu Liu Siyuan Qi Xue Feng Yuxi Ma Yizhou wang Song-Chun Zhu National Key Laboratory of General Artificial Intelligence Beijing Institute for General Artificial IntelligenceBeijing 100086China Institute for Artificial Intelligence Peking UniversityBeijing 100871China Beijing Key Laboratory of Behavior and Mental Health School of Psychological and Cognitive SciencesPeking UniversityBeijing 100871China School of Intelligence Science and Technology Peking UniversityBeijing 100871China School of Computer Science Peking UniversityBeijing 100871China

The release of the generative pre-trained transformer(GPT)series has brought artificial general intelligence(AGI)to the forefront of the artificial intelligence(AI)field once ***,the questions of how to define and evaluate AGI remain *** perspective article proposes that the evaluation of AGI should be rooted in dynamic embodied physical and social interactions(DEPSI).More specifically,we propose five critical characteristics to be considered as AGI benchmarks and suggest the Tong test as an AGI evaluation *** Tong test describes a value-and ability-oriented testing system that delineates five levels of AGI milestones through a virtual environment with DEPSI,allowing for infinite task *** contrast the Tong test with classical AI testing systems in terms of various aspects and propose a systematic evaluation system to promote standardized,quantitative,and objective benchmarks and evaluation of AGI.

关键词： Artificial general intelligence Artificial intelligence benchmark Artificial intelligence evaluation Embodied artificial intelligence Value alignment Turing test Causality

The targeting expression of the vascular endothelial growth factor gene in endothelial cells regulated by ***-1

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Science China(Life Sciences) 2008年第11期51卷 959-965页

作者： ZHENG XiangRong, ZHANG ShangShang, YANG yujia, wang Xia, ZHONG Le & YU XiaoHe Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China Department of Pediatrics Xiangya Hospital Central South University Changsha China

The success of gene therapy depends largely on the efficacy of gene delivery vector systems that can deliver genes to target organs or cells selectively and efficiently with minimal toxicity. Here, we show that by using the ***-1 regulatory element, we were able to restrict expression of the transgene of vascular endothelial growth factor (VEGF) to endothelial cells exclusively in hypoxic conditions. Eukaryotic expression vectors such as ***-1, pcDNA3.1-VEGF+Pa, pcDNA3.1-ppET-1+ EGF+Pa, and ***-1+VEGF+Pa were constructed by using a series of nuclear molecule handling methods like PCR, enzyme digestion. The recombinant vectors were transfected into HUVEC cells and HL7702 cells by the lipofectin method. GFP expression was observed with a fluorescence microscope to validate the specificity of expression in endothelial cells under the regulation of ***-1 element. Cobalt chloride (final concentration 100 μmol/L) was added to the medium to mimic hypoxia in vitro. After transfection of vectors, the expression of VEGF mRNA was detected by RT-PCR, and the expression of VEGF was detected by Western blotting and ELISA methods under normoxia and hypoxia, respectively. The cell proliferation rate was detected by the MTT test. The ex- pression of GFP revealed that the exterior gene was transcripted effectively in endothelial cells regu- lated by the ***-1 element, while the expression of GFP was very weak in nonendothelial cells. The results of RT-PCR, Western blotting and ELISA showed that VEGF gene expression in the ***-1+VEGF+Pa group and in the pcDNA3.1-ppET-1+VEGF+Pa group was higher in hypoxia than it was in normoxia (P<0.05). The MTT test showed that the proliferation rate of HUVEC transfected with HPVA under hypoxia exceeded that of the control group. We conclude that the ***-1 element was expressed specifically in endothelial cells, and can increase the expression of VEGF in hypoxia and stimulate proliferation of

关键词： hypoxia-responsive element human preproendothelin-1 promoter vascular endothelial growth factor endothelial cells

社会情境对情绪感染的影响:一项基于EMG的超扫描研究

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心理学报 2024年第8期56卷 1047-1060页

作者：王丹付雨佳陈文锋中国人民大学心理学系北京100872

情绪感染是社会凝聚力和人际交往的重要催化剂,它能够促进个体间行为、注意力和情绪的同步。传统研究多从个体角度出发,考察社会情境对情绪模仿的影响,但人际情感交流远不止单向模仿。本研究首次采用EMG超扫描技术,结合双人互动范式,模... 详细信息

情绪感染是社会凝聚力和人际交往的重要催化剂,它能够促进个体间行为、注意力和情绪的同步。传统研究多从个体角度出发,考察社会情境对情绪模仿的影响,但人际情感交流远不止单向模仿。本研究首次采用EMG超扫描技术,结合双人互动范式,模拟真实社会互动,探究不同社会情境对情绪同步性的影响及其机制。研究发现,合作和竞争这两种社会背景对情绪感染具有不同的调节作用。在合作情境下,人际间的情绪感染得到加强,观察者体验到更高的情绪强度,且合作组在积极情绪交流中的面部表情同步性高于非合作组。相反,在竞争情境下,情绪感染被削弱,竞争组的观察者体验到更低的情绪强度,面部表情同步性也低于非竞争组。此外,研究还发现,合作和竞争情境激活的依存型和独立型自我建构,分别对面部表情同步性有正向和负向的预测作用。这些发现不仅丰富了我们对生态情境下社会情感互动的认知,也突显了超扫描技术在评估多人视角下人际交流中情绪同步性的重要性。

关键词：情绪同步情绪感染合作竞争自我建构

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教与学信息交互粒子群优化算法

计算机应用 2022年第3期42卷 874-882页

作者：聂方鑫王宇嘉贾欣上海工程技术大学电子电气工程学院上海201620

针对单一种群在解决高维问题中收敛速度较慢和多样性缺失的问题,提出了一种教与学信息交互粒子群优化(PSO)算法。根据进化过程将种群动态地划分为两个子种群,分别采用粒子群优化算法和教与学优化算法,同时粒子利用学习者阶段进行子种群... 详细信息

针对单一种群在解决高维问题中收敛速度较慢和多样性缺失的问题,提出了一种教与学信息交互粒子群优化(PSO)算法。根据进化过程将种群动态地划分为两个子种群,分别采用粒子群优化算法和教与学优化算法,同时粒子利用学习者阶段进行子种群之间信息交互,并通过评价收敛性和多样性指标让粒子的收敛能力和多样性在进化过程中得到平衡。与粒子群优化算法、混合灰狼粒子群算法、重选精英个体的非线性收敛灰狼优化(GWO)算法等多个进化算法在15个标准测试函数的不同维度下进行对比实验,所提算法在多个测试函数上可以收敛到理论最优值,速度相对于其他算法提高了1~6倍。实验结果表明,所提算法在收敛精度和收敛速度上具有较好的效果。

关键词：粒子群优化算法教与学优化算法种群动态调整信息交互归一化方法多种群协同

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知识图谱的增强CP分解链接预测方法

计算机应用研究 2023年第5期40卷 1396-1401页

作者：赵博王宇嘉倪骥上海工程技术大学电子电气工程学院上海201600

CP分解作为知识图谱链接预测的方法之一,能够对一些包含常规数据的知识图谱进行链接预测补全。但当知识图谱存在大量稀疏数据及可逆关系时,该方法不能体现两个实体间具有的隐藏联系,无法对此类数据进行处理。为解决上述问题,提出增强CP... 详细信息

CP分解作为知识图谱链接预测的方法之一,能够对一些包含常规数据的知识图谱进行链接预测补全。但当知识图谱存在大量稀疏数据及可逆关系时,该方法不能体现两个实体间具有的隐藏联系,无法对此类数据进行处理。为解决上述问题,提出增强CP分解方法,对三元组中前实体和后实体的两个嵌入向量分别进行学习,并在训练过程中使用概率方法生成更高质量的负例三元组,引入ELU损失函数和AMSGrad优化器,有效对可逆关系和稀疏数据进行处理。在通用数据集上的实验结果表明,所提方法可以有效提升链接预测精度,与对比模型相比取得了5%的性能提升,同时应用在汽车维修知识图谱数据集补全中,取得83.2%正确率的实体补全结果。

关键词：知识图谱链接预测 CP分解知识图谱嵌入知识图谱补全

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基于语音合成的机器翻译机器人设计

自动化与仪器仪表 2023年第4期 185-190页

作者：王雨佳西安邮电大学西安710000

针对传统翻译机器人在嘈杂环境下无法准确识别语音,导致机器翻译效果不佳的问题,提出设计一个基于语音合成的机器翻译机器人。首先,采用翻译机器人语音系统中的语音硬件设备进行语音数据采集;然后通过语音信号预处理方法分别进行数据预... 详细信息

针对传统翻译机器人在嘈杂环境下无法准确识别语音,导致机器翻译效果不佳的问题,提出设计一个基于语音合成的机器翻译机器人。首先,采用翻译机器人语音系统中的语音硬件设备进行语音数据采集;然后通过语音信号预处理方法分别进行数据预加重、分帧加窗和端点检测;之后利用语言模型进行语音信号特征提取;最后对翻译机器人语音进行编码处理,以实现精准识别。实验结果表明,在采样频率为800 Hz的声卡录音实验中,改进的双门限算法进行端点检测的所用时间仅为0.17 s,识别率高达96.78%,对比于现有的双门限算法的所用时间低了4.78 s,识别率高出了33.36%。在语音识别系统测试中,本系统对合成语音的识别率保持在90%及以上,识别率较高。由此说明,本系统进行语音识别的所用时间更短,检测正确率更高,为翻译机器人语音交互提供了有效的数据支撑。

关键词：语音合成翻译机器人双门限算法端点检测递归神经网络

SAPO-34分子筛的合成及影响因素研究进展

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石油化工 2022年第2期51卷 181-189页

作者：谢萱白英芝王钰佳王海彦辽宁石油化工大学石油化工学院辽宁抚顺113001 中国石油抚顺石化分公司辽宁抚顺113008

低碳烯烃是十分重要的工业化工原料,尤其是乙烯和丙烯,市场需求量越来越大,而SAPO-34分子筛是甲醇制烯烃工艺发展的决策性因素。介绍了SAPO-34分子筛的合成方法和影响因素,通过调变SAPO-34分子筛的组成、模板剂、合成条件等因素可制备... 详细信息

低碳烯烃是十分重要的工业化工原料,尤其是乙烯和丙烯,市场需求量越来越大,而SAPO-34分子筛是甲醇制烯烃工艺发展的决策性因素。介绍了SAPO-34分子筛的合成方法和影响因素,通过调变SAPO-34分子筛的组成、模板剂、合成条件等因素可制备出符合工业要求的催化剂。对SAPO-34分子筛的发展方向进行了展望,高性能低污染的SAPO-34分子筛的制备方式将是未来的研究重点。

关键词： SAPO-34分子筛合成方法影响因素改性

Single-cell RNA-Seq analysis identified kidney progenitor cells from human urine

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Protein & Cell 2021年第4期12卷 305-312页

作者： yujia wang Yu Zhao Zixian Zhao Dandan Li Hao Nie Yufen Sun Xiaobei Feng Ting Zhang Yu Ma Jing Nie Guangyan Cai Xiangmei Chen Wei Zuo East Hospital School of MedicineTongji UniversityShanghai 200092China Regend Therapeutics Co.Ltd. Zhangjiang Hi-tech ParkShanghai 201203China Ningxia Medical University Yinchuan 750004China The First Affiliated Hospital Guangzhou Medical UniversityGuangzhou 510120China Ruijin Hospital School of MedicineShanghai Jiaotong UniversityShanghai 200025China State Key Laboratory of Organ Failure Research National Clinical Research Center of Kidney DiseaseKey Laboratory of Organ Failure ResearchDivision of NephrologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515China National Clinical Research Center for Kidney Diseases State Key Laboratory of Kidney DiseasesChinese PLA General HospitalBeijing 100039China

Dear Editor,Urine passes through the entire kidney and urinary tract system starting from the glomerulus and ending to the *** in the kidney and urinary tract could be exfoliated from the epithelium into the urine,while leukocyte could infiltrate from the local tissue into the urine,which makes the urine a useful subject for clinical evaluation of relevant diseases.

关键词： urine kidney urinary