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Function of hepatocyte spheroids in bioactive microcapsules is enhanced by endogenous and exogenous hepatocyte growth factor

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Bioactive Materials 2023年第10期28卷 183-195页

作者： Kihak Gwon Daheui Choi Jose Mde Hoyos-Vega Harihara Baskaran Alan M.Gonzalez-Suarez Seonhwa Lee Hye Jin Hong Kianna M.Nguyen Ether Dharmesh Go Sugahara yuji ishida Takeshi Saito Gulnaz Stybayeva Alexander Revzin Department of Physiology and Biomedical Engineering Mayo ClinicRochesterMNUSA Department of Chemical and Biomolecular Engineering Case Western Reserve UniversityClevelandOHUSA Biomedical Engineering Saint Louis UniversitySt.LouisMOUSA University of Southern Californi1 Keck School of MedicineDepartment of MedicineDivision of Gastrointestinal and Liver DiseasesLos AngelesCAUSA Research and Development Department PhoenixBioCo.LtdKagamiyam1Higashi-Hiroshim1Hiroshim1Japan USC Research Center for Liver Diseases Los AngelesCAUSA

The ability to maintain functional hepatocytes has important implications for bioartificial liver development,cell-based therapies,drug screening,and tissue engineering.Several approaches can be used to restore hepatocyte function in vitro,including coating a culture substrate with extracellular matrix(ECM),encapsulating cells within biomimetic gels(Collagen-or Matrigel-based),or co-cultivation with other cells.This paper describes the use of bioactive heparin-based core-shell microcapsules to form and cultivate hepatocyte spheroids.These microcapsules are comprised of an aqueous core that facilitates hepatocyte aggregation into spheroids and a heparin hydrogel shell that binds and releases growth factors.We demonstrate that bioactive microcapsules retain and release endogenous signals thus enhancing the function of encapsulated hepatocytes.We also demonstrate that hepatic function may be further enhanced by loading exogenous hepatocyte growth factor(HGF)into microcapsules and inhibiting transforming growth factor(TGF)-β1 signaling.Overall,bioactive microcapsules described here represent a promising new strategy for the encapsulation and maintenance of primary hepatocytes and will be beneficial for liver tissue engineering,regenerative medicine,and drug testing applications.

关键词： Droplet microfluidics Bioactive microcapsules Hepatocyte spheroid Heparin Hepatocyte growth factor

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A new approach to transfect NF-κB decoy oligodeoxynucleotides into the periodontal tissue using the ultrasound-microbubble method

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International Journal of Oral Science 2017年第2期9卷 80-86页

作者： Hiroyuki Yamaguchi yuji ishida Jun Hosomichi Jun-ichi Suzuki Risa Usumi-Fujita Yasuhiro Shimizu Sawa Kaneko Takashi Ono Department of Orthodontic Science Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityTokyoJapan Department of Advanced Clinical Science and Therapeutics Graduate School of MedicineThe University of TokyoTokyoJapan

The objective of this study is to investigate the effect of the ultrasound-microbubble technique in nuclear factor kappa B(NF-κB) decoy oligodeoxynucleotide(ODN) transfection in the gingival tissue in mice. The 6-FAM-labeled scrambled decoy ODN with microbubbles was applied to the periodontal tissue in 8-week-old male C57BL/6J mice by ultrasound radiation at low(LUM-Sc) and high(HUM-Sc) intensities to optimize the transfection condition of the ultrasound-microbubble method.Histological inspections were performed two hours after transfection to compare the expression with that in the sham-operated group without ultrasound radiation(A-Sc). Then, an NF-κB decoy was transfected into the periodontal tissue using the highintensity ultrasound-microbubble(HUM-NF) technique to examine the anti-inflammatory effects of the decoy ODN. Western blot analysis was performed to investigate the expression of interleukin(IL)-1β, IL-6 and intercellular adhesion molecule-1(ICAM-1)in the gingival tissues in the HUM-Sc, the HUM-NF and control groups. The fluorescence microscopy results showed that the fluorescent intensity in the periodontal tissues in the LUM-Sc and HUM-Sc groups was significantly higher than that in the A-Sc and the control groups. The fluorescent intensity in the HUM-Sc group, especially in the gingival connective tissue,was the highest of all groups. Western blot analysis indicated that the protein expression levels of IL-1β, IL-6 and ICAM-1 in the HUM-NF group were significantly lower than those in the HUM-Sc and the control groups. These findings suggest that the high-intensity ultrasound-microbubble technique is an effective tool for decoy transfection into the periodontal tissue.

关键词： decoy oligodeoxynucleotide gene therapy periodontal tissue ultrasound

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Martensitic transformation and shape memory effect in ausaged Fe-Ni-Si-based alloys

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材料与冶金学报 2005年第2期4卷 109-109页

作者：田中优树 HIMURO Yoshiyuki SUTOU yuji KAINUMA Ryosuke ishida Kiyohito 日本东北大学 Department of Material Science Graduate School of Engineering Tohoku UniversityAramaki Aoba-yama 6-6-02 Sendai 980-8579 Japan Biomedical Engineering Research Organizations Tohoku University Sendai 980-8575Japan Department of Material Science

It is well known that the morphologies of the α’ martensite formed from the γ phase in ferrous alloys are classified into five types of lath, butterfly, （225）A type plate,lenticular and thin-plate. Among those α’ martensites, only the thin-plate martensite,which is characterized by containing a high density of transformation twins, has a potential of exhibiting a perfect shape memory （SM） effect.Recently the present authors found in Fe-Ni-Si alloys that the thin-plate martensite is formed by the introduction of fine and coherent γ’-（Ni,Fe）3Si particles with a L12 ordered structure in the austenite matrix due to ausaging. In the present study, the SM properties of the ausaged Fe-Ni-Si alloys with the thin-plate martensite are investigated by a conventional bending-test. The effects of the addition of Co to the Fe-Ni-Si alloys on the martensitic transformation and the SM properties are also investigated. It is shown that while the ausaged Fe-Ni-Si ternary alloys exhibit an imperfect SM effect due to reverse transformation from stress-induced thin-plate martensite to austenite, the SM properties are improved by the addition of Co. An almost perfect SM effect is confirmed in the Fe-Ni-Si-Co alloys by heating to 1 100 ℃ after deformation at -196 ℃.

关键词： Fe-Ni-Si基奥氏体时效合金马氏体相变形状记忆效应薄钢板

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FCC/HCP martensitic transformation and shape memory effect in Co-Al binary system

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材料与冶金学报 2005年第2期4卷 120-120页

作者：大森俊洋 SUTOU yuji OIKAWA Katsunari KAINUMA Ryosuke ishida Kiyohito 日本东北大学 Tohoku University Biomedical Engineering Research Organization 2-1 Seiryo-machiSendai 980-8575 Japan Department of Materials Science Graduate School of Engineering Tohoku University Sendai 980-8579 Japan Department of Materials Science

It is known that pure Co undergoes martensitic transformation from γ phase (fcc) to ε phase (hcp) by the movement of a/6 Shockley partial dislocations at around 400 ℃, however, there have been few systematic works on the SM effect in Co and Co-based alloys. In this study, the fcc/hcp rnartensitic transformation and the SM effect were investigated in Co-A1 binary alloys(mole fraction of Al=0-16%). The γ/ε rnartensitic transformation temperatures were found from the DSC measurements to decrease with increasing Al content, while the transformation temperature hystereses were observed to increase from 60℃ at x(Al)=0 to 150℃ at x(Al) = 16%. The SM effect evaluated by a conventional bending test was enhanced by the addition of Al over 4% (mole fraction) and Co-Al alloys containing over 10%(mole fraction) exhibit a good SM effect associated with the hcpfee → reverse transformation above 200℃. The SM effect was significantly improved by precipitation of β (I32) phase and the max[real shape recovery strain of 2.2 % was obtained, which can be explained by precipitation hardening. The crystallographic orientations between the β, εand γ phases were also determined. Finally, the magnetic properties were investigated and it was found that the Curie temperature and saturation magnetization of Co-14% Al(mole fraction) are 690℃ and 120 emu/g, respectively. It is concluded that the Co-A1 alloys hold promise as new high-temperature and ferromagnetic SM alloys.

关键词： FCC/HCP 马氏体相变形状记忆效应 Co-Al二元系

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Martensitic and magnetic transformations of Ni-Ga-Fe-Co ferromagnetic shape memory alloys

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材料与冶金学报 2005年第2期4卷 121-121页

作者：今野阳介 OIKAWA Katsunari OMORI Toshihiro SUTOU yuji KAINUMA Ryosuke ishida Kiyohito 日本东北大学大学院 Department of Materials Science Graduate School of Engineering Tohoku UniversityAoba-yama 6-6-02 Sendai 980-8579 Japan Tohoku University Biomedical Engineering Research Organization 2-1 Seiryo-machiAoba-ku Sendai 980-8575 Japan Department of Materials Science

Ferromagnetic shape memory alloys （FSMAs） such as NiMnGa, FePd and FePt are attractive as a new magnetic actuator material. They show a large magnetic-field-induced strain of 3% 9% due to the variant rearrangement. Recently, the present authors have reported that in the Ni-Ga-Fe alloy the martensitic transformationfrom the B2 and/or the L21 structures into a seven-layer or five-layer modulated structure occurs upon cooling. In this alloy system, however, it is impossible to obtain a martensite phase at RT with a Curie temperature （TC） higher than 100 ℃. In this work,the effects of substitution of Co for Ni on the martensitic and magnetic transformations,crystal structures and phase equilibria in Ni-Ga-Fe alloys were studied.Ni-Ga-Fe-Co alloys were prepared by induction melting under an argon atmosphere.Small pieces of specimens were taken from the ingot and homogenized at 1433 K for 24 h followed by quenching in water. The obtained specimens were aged at 773 K for 24 h and then quenched. The compositions of each phase were determined by energy dispersion X-ray spectroscopy （EDX）. The martensitic transformation temperatures and TC were measured by differential scanning calorimetry （DSC） and vibrating sample magnetometer （VSM） measurement. The crystal structure of martensite phase was observed by X-ray diffractmeter （XRD） and transmission electron microscope （TEM）.The Curie temperature TC was increased with increasing Co content while the martensitic transformation temperature slightly decreased. In the Ni54-x Ga27 Fe19 Cox ,TC increases from 303 K to 408 K with increasing Co content from x=0 to x=6. The crystal structure of the martensite phase and the phase equiribria in the Ni-Fe-Ga-Co alloys will be also presented.

关键词： Ni-Ga-Fe-Co铁磁形状记忆合金磁性转变马氏体相变 SMA 居里温度

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Effect of texture on internal friction of Cu-Al-Mn based shape memory alloys

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材料与冶金学报 2005年第2期4卷 91-91页

作者：须藤祐司 OMORI Toshihiro KOEDA Naoki KAINUMA Ryosuke ishida Kiyohito 日本东北大学 Biomedical Engineering Research Organization Tohoku University 2-1 Seiryo-machi Aoba-ku Sendai 980-8575 Japan Department of Metallurgy Graduate School of Engineering Tohoku UniversityAoba-yama 6-6-02 Sendai 980-8579 Japan Department of Metallurgy

Shape memory alloys (SMAs) are well-known as high damping materials. Recently,we have reported that the Cu-Al-Mn SMAs show a high internal friction as well as the other SMAs such as Ni-Ti, Cu-Zn-Al SMAs. Since the Cu-Al-Mn-based SMAs possess an excellent cold-workability, a strong recrystallization texture can be formed by thermomechanical treatment. The present authors demonstrated that the texture control is considerably effective for improving the SM properties and especially, in the Cu-Al-Mn-Ni SMAs with a strong {112} recrystallization texture, a large pseudoelastic (PE) of about 7% can be obtained. Such a texture control is expected to enhance the damping capacity in the Cu-Al-Mn-based SMAs as well as PE. In this study, the effect of texture on the internal friction in Cu-Al-Mn and Cu-Al-Mn-Ni SMAs was investigated by tensile testing mode of Dynamic Mechanical Spectrometer (DMS). In sheet specimens with the {112} texture, value of dynamic damping tanφ depends on the loading direction and an excellent damping capacity of tanφ= 0.1 can be obtained at the rolling direction in the martensite phase condition. Moreover, these Cu-Al-Mn-based SMAs show a high tensile strength over 600 MPa in the martensite phase. The present Cu-Al-Mn-based SMAs should have a great potential as the damping materials.

关键词： Cu-Al-Mn系形状记忆合金内耗结构

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