检索结果-南通市图书馆

The microgravity enhanced polymer-mediated siRNA gene silence by improving cellular uptake

Biophysics Reports 2020年第6期6卷 266-277页

作者： Tongren Yang Chanchan Yu Changrong Wang Chunhui Li Mengjie Zhang Xiaofan luo yuhua Weng Anjie Dong Xiaoqiong Li Yulin Deng Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary ScienceInstitute of Engineering MedicineKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of Technology Department of Polymer Science and Technology School of Chemical Engineering and TechnologyKey Laboratory of Systems Bioengineering of the Ministry of EducationTianjin University School of Pharmacy Hunan University of Chinese Medicine

Microgravity(MG) effect is a weightlessness phenomenon caused by the distance from the ground or low gravity of other planets outside the earth”s atmosphere. The various effects of MG have been corroborated in human and animal studies and modeled in cell-based analogs. However, the impact of MG on siRNA performance remains to be elucidated, which is crucial for aerospace medicine. In this study, we prepared nucleic acid nanomicelles(EAASc/siRNA) by using tri-block copolymer of PEG45-PAMA40-P(C7 A36-DBA37)(EAASc) and siRNA and explored its working mechanism under simulated microgravity(SMG) condition generated by a random positioning machine(RPM). The binding ability of EAASc to siRNA and silence activity were firstly confirmed in normal gravity(NG) *** of PLK1 mRNA expression revealed that gene inhibition efficiencies were increased by28.7%(HepG2) and 28.9%(A549) under SMG condition, compared with those under NG condition. In addition, mechanism exploration indicated that morphology and migration capability of cancer cells were significantly changed, the internalization of EAASc/siRNA by cells was magnified when the cells were incubated with RPM. No significant difference was observed regarding the expression profiles of genes involved in RNA interference(RNAi) pathway, including Ago2, Dicer, TRBP, and so on. Taken together, siRNA activity was elevated under SMG condition owning to increased cellular *** study, for the first time to our knowledge, provides valuable theory for development and application of siRNA therapeutic in space in the future.

关键词： Polymer siRNA PLK1 Microgravity Space pharmaceutics Aerospace medicine

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Fabrication of highly dispersed platinum-deposited porous g-C_3N_4 by a simple in situ photoreduction strategy and their excellent visible light photocatalytic activity toward aqueous 4-fluorophenol degradation

引用

Chinese Journal of Catalysis 2017年第1期38卷 29-38页

作者： Zhenxing Zeng Kexin Li Kai Wei yuhua Dai Liushui Yan Huiqin Guo Xubiao luo Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle Nanchang Hangkong University Nanchang 330063 JiangxiChina

A series of highly dispersed platinum‐deposited porous g‐C3N4 (Pt/pg‐C3N4) were successfully fabricated by a simple in situ photoreduction strategy using chloroplatinic acid and porous g‐C3N4 as precursors. Porous g‐C3N4 was fabricated by a pretreatment strategy using melamine as a raw *** morphology, porosity, phase, chemical structure, and optical and electronic properties ofas‐prepared Pt/pg‐C3N4 were characterized. The photocatalytic activity of as‐prepared Pt/pg‐C3N4was preliminarily evaluated by the degradation of aqueous azo dyes methyl orange under visible light irradiation. The as‐prepared Pt/pg‐C3N4 were further applied to the degradation and mineralization of aqueous 4‐fluorophenol. The recyclability of Pt/pg‐C3N4 was evaluated under four consecutive photocatalytic runs.

关键词： Photocatalysis Porous graphitic carbon nitride Photoreduction Platinum deposition 4‐Fluorophenol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synergistic photocatalytic effect of porous g-C_3N_4 in a Cr(Ⅵ)/4-chlorophenol composite pollution system

引用

Chinese Journal of Catalysis 2017年第11期38卷 1804-1811页

作者： Kai Wei Kexin Li Zhenxing Zeng yuhua Dai Liushui Yan Huiqin Guo Xubiao luo Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle Nanchang Hangkong University Nanchang 330063 JiangxiChina

The photocatalytic reduction of aqueous Cr(VI)to Cr(III)was preliminarily studied using porousg‐C3N4as a photocatalyst under acidic *** observed synergistic photocatalytic effect ofporous g‐C3N4on a Cr(VI)/4‐chlorophenol(4‐CP)composite pollution system was further studiedunder different pH *** with single‐component photocatalytic systems for Cr(VI)reduction or4‐CP degradation,the Cr(VI)reduction efficiency and4‐CP degradation efficiency weresimultaneously improved in the Cr(VI)/4‐CP composite pollution *** synergistic photocatalyticeffect in the Cr(VI)/4‐CP composite pollution system can be attributed to the acceleratedredox reaction between dichromate and4‐CP by electron transfer with porous g‐C3N4.

关键词： Composite pollution Synergistic photocatalysis Porous g‐C3N4 Cr(VI) 4‐Chlorophenol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

油气勘探开发投资优化

引用

石油勘探与开发 2009年第4期36卷 535-540页

作者：郑玉华罗东坤中国石油大学(北京)工商管理学院

以探明储量增长模型为基础建立了油气累计勘探投资与油气累计探明储量之间的关系模型;用数据包络分析法(DEA)建立了油气新建产能-油气开发工程建设投资模型。结合对数形式的油气开发操作成本模型构建了油公司油气开发投资-油气产量的关... 详细信息

以探明储量增长模型为基础建立了油气累计勘探投资与油气累计探明储量之间的关系模型;用数据包络分析法(DEA)建立了油气新建产能-油气开发工程建设投资模型。结合对数形式的油气开发操作成本模型构建了油公司油气开发投资-油气产量的关系模型;在投资-储量-产量关系模型的基础上,以企业利润最大化为目标,考虑产量构成、储采比及开采速度等方面的约束条件,构造了石油公司勘探开发投资多阶段优化模型,并用遗传算法给出了既定条件下石油公司多阶段最优化投资结构。该方法可以为石油公司制定长期发展战略规划、长期勘探开发投资方案提供方法依据,以减小勘探、开发投资中的盲目性及主观性,促进油气资源的合理、有序勘探开发。

关键词：勘探开发投资决策投资优化油气储量油气产量

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The H3K9me2-binding protein AGDP3 limits DNA methylation and transcriptional gene silencing in Arabidopsis

引用

Journal of Integrative Plant Biology 2022年第12期64卷 2385-2395页

作者： Xuelin Zhou Mengwei Wei Wenfeng Nie Yue Xi Li Peng Qijie Zheng Kai Tang Viswanathan Satheesh yuhua Wang Jinyan luo Xuan Du Rui Liu Zhenlin Yang Honggui La Yingli Zhong Yu Yang Jian-Kang Zhu Jiamu Du Mingguang Lei Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China Department of Horticulture College of Horticulture and Plant ProtectionYangzhou UniversityYangzhou 225009China Department of Horticulture&Landscape Architecture Purdue UniversityWest LafayetteIdiana47906USA Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Institute of Plant and Food ScienceSchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China College of Bioscience and Biotechnology Hunan Agricultural UniversityChangsha 410128China

DNA methylation,a conserved epigenetic mark,is critical for tuning temporal and spatial gene *** Arabidopsis thaliana DNA glycosylase/lyase REPRESSOR OF SILENCING 1(ROS1)initiates active DNA demethylation and is required to prevent DNA hypermethylation at thousands of genomic ***,how ROS1 is recruited to specific loci is not well ***,we report the discovery of Arabidopsis AGENET Domain Containing Protein 3(AGDP3)as a cellular factor that is required to prevent gene silencing and DNA ***3 binds to H3K9me2 marks in its target DNA via its AGD12 *** of the crystal structure of the AGD12 cassette of AGDP3 in complex with an H3K9me2 peptide revealed that dimethylated H3 K9 and unmodified H3 K4 are specifically anchored into two different surface pockets.A histidine residue located in the methyllysine binding aromatic cage provides AGDP3 with pH-dependent H3K9me2 binding *** results uncover a molecular mechanism for the regulation of DNA demethylation by the gene silencing mark H3K9me2.

关键词： AGDP3 DNA demethylation epigenetics H3K9me2 ROS1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

羟考酮复合左布比卡因用于硬膜外分娩镇痛的尝试性研究

引用

中国现代应用药学 2020年第1期37卷 96-99页

作者：罗苹项余华徐峰衢州市中医医院麻醉科浙江衢州324000 嘉兴悦程妇产医院麻醉科浙江嘉兴314000

目的探讨羟考酮复合左布比卡因用于硬膜外分娩镇痛的安全性和效果。方法100例单胎足月初产妇随机分为2组:观察组50例采用0.2 mg·mL^-1羟考酮复合0.1%左布比卡因硬膜外分娩镇痛;对照组50例采用2μg·mL^-1芬太尼复合0.1%左布比卡因硬膜... 详细信息

目的探讨羟考酮复合左布比卡因用于硬膜外分娩镇痛的安全性和效果。方法100例单胎足月初产妇随机分为2组:观察组50例采用0.2 mg·mL^-1羟考酮复合0.1%左布比卡因硬膜外分娩镇痛;对照组50例采用2μg·mL^-1芬太尼复合0.1%左布比卡因硬膜外分娩镇痛。观察2组镇痛起效时间、镇痛持续时间、产程时间、分娩方式、血压及镇痛效果,评估新生儿Apgar评分,采集脐动脉血进行血气分析,并记录母婴并发症。结果2组产妇镇痛后疼痛视觉模拟评分(VAS)均显著低于镇痛前(P<0.01),但是2组间第一产程中VAS评分比较有统计学意义(P<0.05)。2组产妇镇痛起效时间、产程时间、分娩方式、Bromage评分、新生儿Apgar评分、脐动脉血气比较均无统计学意义,但观察组镇痛持续时间明显长于观察组[(6.2±1.4)h vs(1.1±0.3)h,P<0.01]。观察组产妇皮肤瘙痒发生率明显高于对照组(16%vs 2%,P<0.05)。结论羟考酮复合左布比卡因硬膜外分娩镇痛效果优于芬太尼,且作用时间久,不影响产程,对新生儿无明显影响,但皮肤瘙痒发生率明显增加。

关键词：羟考酮左布比卡因硬膜外分娩镇痛

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

温度对染料掺杂胆甾相液晶激光波长的影响及应用

引用

中国激光 2016年第1期43卷 130-135页

作者：叶丽慧黄玉华罗吉祥浙江师范大学信息光学研究所浙江省光信息检测与显示技术研究重点实验室浙江金华321004

研究了温度对激光染料掺杂胆甾相液晶的激光波长的影响,发现胆甾相液晶输出的激光波长总体上随温度的升高而红移,但是不同厚度的样品随温度变化的趋势略有不同。样品较薄时,激光波长随温度变化呈周期性的跳变式增长,而在相邻两个跳变温... 详细信息

研究了温度对激光染料掺杂胆甾相液晶的激光波长的影响,发现胆甾相液晶输出的激光波长总体上随温度的升高而红移,但是不同厚度的样品随温度变化的趋势略有不同。样品较薄时,激光波长随温度变化呈周期性的跳变式增长,而在相邻两个跳变温度之间的一小段温度范围内则随温度的升高而蓝移,且激光波长随温度变化产生突变的周期随样品厚度增加而减小。当样品厚度足够大时,激光波长随温度变化产生的突变消失,几乎随温度呈线性增长。分析了产生该现象的物理机制,并对其进行了理论模拟。模拟结果与实验结果一致。厚的染料掺杂胆甾相液晶样品产生的激光波长随温度线性变化的这一特性,可以用于温度传感。

关键词：材料胆甾相液晶液晶激光温度传感器波长厚度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

引用

Cell Regeneration 2013年第1期2卷 42-49页

作者： Jinglei Cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu yuhua Sun Ang Li Ke Huang Rongping luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei Guangjin Pan Duanqing Pei CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell Biology and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institute of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan AvenueScience ParkGuangzhou 510530P.R.China Department of Regeneration Medicine School of PharmaceuticalJilin UniversityChangchunP.R.China Department of Oral and Maxillofacial Surgery Laboratory of Interdisciplinary StudiesSchool and Hospital of StomatologyPeking UniversityBeijingP.R.China Biodynamic Optical Imaging Center(BIOPIC) Peking UniversityBeijingP.R.China The Shenzhen Key Lab of Gene and Antibody Therapy Center for Biotech&Biomedicine and Division of Life SciencesGraduate School at ShenzhenTsinghua UniversityShenzhenP.R.China Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingP.R.China.

Background:Tooth is vital not only for a good smile,but also good ***,we lose tooth regularly due to accidents or *** ideal solution to this problem is to regenerate tooth with patients’own *** we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental ***-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 *** further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human ***:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies.

关键词： Human urine Integration-free iPSCs Recombinant tooth Bioengineered tooth Dental epithelium

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

二级回火对(NdDy)FeCoCuB磁体磁性能和微结构的影响

引用

稀有金属材料与工程 2005年第10期34卷 1633-1636页

作者：王伟罗伟文玉华于濂清严密浙江大学硅材料国家重点实验室浙江杭州310027

研究了二级回火工艺对Nd12.0Dy2.8Fe74.7Co3.4Cu0.2B6.9磁体磁性能和微结构的影响。研究结果表明:二级回火对磁体的剩磁和磁能积影响很小,内禀矫顽力Hcj随二级回火温度升高而增大,在480℃时达到最大;高于480℃后,Hcj随二级回火温度的升... 详细信息

研究了二级回火工艺对Nd12.0Dy2.8Fe74.7Co3.4Cu0.2B6.9磁体磁性能和微结构的影响。研究结果表明:二级回火对磁体的剩磁和磁能积影响很小,内禀矫顽力Hcj随二级回火温度升高而增大,在480℃时达到最大;高于480℃后,Hcj随二级回火温度的升高而迅速下降;在480℃二级回火时,Hcj随回火时间的增加而增大,经2h回火达到最大;大于2h后,回火时间的进一步增加对Hcj影响很小;480℃二级回火2h时磁体主相晶粒边界光滑,富稀土相在晶界分布连续、均匀。

关键词：烧结钕铁硼二级回火磁性能富稀土相

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Experimental Evidence of Topological Surface States in Mg3Bi2 Films Grown by Molecular Beam Epitaxy

引用

Chinese Physics Letters 2019年第11期36卷 65-69页

作者： Tong Zhou Xie-Gang Zhu Mingyu Tong Yun Zhang Xue-Bing luo Xiangnan Xie Wei Feng Qiuyun Chen Shiyong Tan Zhen-Yu Wang Tian Jiang yuhua Tang Xin-Chun Lai Xuejun Yang State Key Laboratory of High Performance Computing College of ComputerNational University of Defense TechnologyChangsha 410073 Science and Technology on Surface Physics and Chemistry Laboratory Jiangyou 621908 National Innovation Institute of Defense Technology Academy of Military Sciences PLA ChinaBeijing 100010 Institute of Materials China Academy of Engineering PhysicsMianyang 621700 College of Advanced Interdisciplinary Studies National University of Defense TechnologyChangsha 410073 Academy of Military Sciences PLA China Beijing 100010 Beijing Academy of Quantum Information Sciences Beijing 100084

Nodal line semimetal(NLS) is a new quantum state hosting one-dimensional closed loops formed by the crossing of two bands. The so-called type-Ⅱ NLS means that these two crossing bands have the same sign in their slopes along the radial direction of the loop, which requires that the crossing bands are either right-tilted or left-tilted at the same time. According to the theoretical prediction, Mg3Bi2 is an ideal candidate for studying the type-Ⅱ NLS by tuning its spin-orbit coupling(SOC). High-quality Mg3 Bi2 films are grown by molecular beam epitaxy(MBE). By in-situ angle resolved photoemission spectroscopy(ARPES), a pair of surface resonance bands around theГ point are clearly seen. This shows that Mg3Bi2 films grown by MBE are Mg(1)-terminated by comparing the ARPES spectra with the first principles calculations results. Moreover, the temperature dependent weak anti-localization effect in Mg3Bi2 films is observed under magneto-transport measurements, which shows clear two-dimensional(2 D) e-e scattering characteristics by fitting with the Hikami–Larkin–Nagaoka model. Therefore, by combining with ARPES, magneto-transport measurements and the first principles calculations, this work proves that Mg3Bi2 is a semimetal with topological surface states. This paves the way for Mg3Bi2 to be used as an ideal material platform to study the exotic features of type-Ⅱ nodal line semimetals and the topological phase transition by tuning its SOC.

关键词： bands topological terminated

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：