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Fluorescent Double Network Hydrogels with Ionic Responsiveness and High Mechanical Properties for Visual Detection

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Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 487-496页

作者：郑湾 LIU Lerong Lü Hanlin WANG yuhang LI Feihu ZHANG Yixuan 陈艳军 WANG Yifeng School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China

We developed a fluorescent double network hydrogel with ionic responsiveness and high mechanical properties for visual *** nanocomposite hydrogel of laponite and polyacrylamide serves as the first network,while the ionic cross-linked hydrogel of terbium ions and sodium alginate serves as the second *** double-network structure,the introduction of nanoparticles and the reversible ionic crosslinked interactions confer high mechanical properties to the *** ions are not only used as the ionic cross-linked points,but also used as green emitters to endow hydrogels with fluorescent *** the basis of the “antenna effect” of terbium ions and the ion exchange interaction,the fluorescence of the hydrogels can make selective responses to various ions(such as organic acid radical ions,transition metal ions) in aqueous solutions,which enables a convenient strategy for visual detection toward ***,the fluorescent double network hydrogel fabricated in this study is promising for use in the field of visual sensor detection.

关键词： visual detection ionic responsiveness fluorescent hydrogels double network hydrogels mechanical property

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复合酶解法优化黄精多糖提取工艺

食品与生物技术学报 2017年第9期36卷 996-1001页

作者：苑璐冷凯良周余航周三青岛大学药学院山东青岛266021 中国水产科学研究院黄海水产研究所山东青岛266071 烟台大学药学院山东烟台264005

采用复合酶解法优化黄精多糖提取工艺,苯酚-浓硫酸显色法测定黄精多糖质量浓度,以黄精多糖提取率为指标,对复合酶种类和配比进行筛选后,在单因素试验基础上,考察酶解温度、pH、料液比、加酶量对提取率的影响,并通过正交试验进行优化。... 详细信息

采用复合酶解法优化黄精多糖提取工艺,苯酚-浓硫酸显色法测定黄精多糖质量浓度,以黄精多糖提取率为指标,对复合酶种类和配比进行筛选后,在单因素试验基础上,考察酶解温度、pH、料液比、加酶量对提取率的影响,并通过正交试验进行优化。结果表明,复合酶提取优于单酶提取和普通水提。酶用量配比为纤维素酶:木瓜蛋白酶=3∶7。酶解最佳条件为:pH值5.0,酶解温度50℃,料液比(g/mL)1∶20,加酶量1.5 g/dL,即纤维素酶0.45 g/dL,木瓜蛋白酶1.05 g/dL,酶解2 h后,沸水浸提2 h。在此工艺条件下,黄精多糖提取率可达21.55%,是普通水提法得率的2.75倍,比单酶水解高出12.06%。

关键词：黄精多糖复合酶提取正交试验

Electric-controlled pressure relief valve for enhanced safety in liquid-cooled lithium-ion battery packs

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Journal of Energy Chemistry 2024年第3期90卷 98-109,I0004页

作者： yuhang Song Jidong Hou Nawei Lyu Xinyuan Luo Jingxuan Ma Shuwen Chen Peihao Wu Xin Jiang Yang Jin Research Center of Grid Energy Storage and Battery Application School of Electrical and Information EngineeringZhengzhou UniversityZhengzhou 450001HenanChina

The liquid-cooled battery energy sto rage system(LCBESS) has gained significant attention due to its superior thermal management ***,liquid-cooled battery pack(LCBP) usually has a high sealing level above IP65,which can trap flammable and explosive gases from battery thermal runaway and cause *** poses serious safety risks and challenges for *** this study,we tested overcharged battery inside a commercial LCBP and found that the conventionally mechanical pressure relief valve(PRV) on the LCBP had a delayed response and low-pressure relief efficiency.A realistic 20-foot model of an energy storage cabin was constructed using the Flacs finite element simulation *** studies were conducted to evaluate the pressure relief efficiency and the influence on neighboring battery packs in case of internal explosions,considering different sizes and installation positions of the ***,a newly developed electric-controlled PRV integrated with battery fault detection is introduced,capable of starting within 50 ms of the battery safety valve ***,the PRV was integrated with the battery management system and changed the battery charging and discharging strategy after the PRV was *** tests confirmed the efficacy of this method in preventing *** paper addresses the safety concerns associated with LCBPs and proposes an effective solution for explosion relief.

关键词： Pressure relief valve Liquid-cooled battery pack Explosion Flacs

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“缘何愉悦”--当代西方恐怖艺术悖论研究

中南大学学报（社会科学版） 2020年第3期26卷 157-163页

作者：周宇航杨莉馨南京师范大学文学院江苏南京210097

当代西方对于恐怖艺术悖论的解读存在三种思路:一是以"压抑""强迫性重复""凝视"等概念为出发点分析愉悦成因的精神分析论;二是具体观点存在分歧但都强调愉悦是一种认知型情感并与好奇心紧密相关的好奇论;三是认为愉悦成因复杂所以并不... 详细信息

当代西方对于恐怖艺术悖论的解读存在三种思路:一是以"压抑""强迫性重复""凝视"等概念为出发点分析愉悦成因的精神分析论;二是具体观点存在分歧但都强调愉悦是一种认知型情感并与好奇心紧密相关的好奇论;三是认为愉悦成因复杂所以并不试图寻求某一根本原因而从审美沉浸、现实映射等多方面展开论述的多元路径论。这三种观点彼此之间既冲突又互补,都在某个层面针对某一具体的艺术类型解释了恐怖艺术的悖论。

关键词：恐怖艺术悖论精神分析好奇心多元路径

Impacts of Integrated Multi-Trophic Aquaculture on Phytoplankton in Sanggou Bay

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Journal of Ocean University of China 2024年第3期23卷 835-843页

作者： HAN Jichang LIU Xing PAN Kehou LIU Jiaojiao SUN Yan JIN Guiyong LI Yun LI yuhang College of Food Science and Engineering Ningbo UniversityNingbo 315832China Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266100China Laboratory of Marine Organism Taxonomy and Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Integrated multi-trophic aquaculture(IMTA)has been considered as an ecofriendly culture system providing a potential solution to environmental risks caused by intensive monoculture ***,the impact of IMTA on phytoplankton remains *** this study,the spatial and temporal variations of phytoplankton in Sanggou Bay were investigated seasonally based on 21 sampling sites covering three cultivation zones(bivalve zone,IMTA zone,and kelp zone)and one control zone(without aquatic cultivation).In total,128 phytoplankton species,with diatoms and dinoflagellates as the dominant groups,were obtained across the whole year,and the mean Shannon diversity index(H')and species richness(SR)were determined as 1.39 and 9.39,*** maximum chlorophyll a(Chl-a)(6.32μg L^(-1))and plankton diversity(H'of 1.97)occurred in summer and autumn,*** to other zones,the bivalve zone displayed significantly higher Chl-a and lower H'in majority of *** PERMANOVA analysis indicated that the phytoplankton assemblage in the bivalve zone was significantly different with the control and kelp zones,while the IMTA zone maintained close to other three *** on generalized additive models,temperature,NO_(2)^(-)-N,N/P ratio,SiO_(3)^(2-)-Si,and salinity were determined as the key factors underlying Chl-a and phytoplankton ***-tionally,the results of redundancy analysis further indicated that the phytoplankton assemblage in the bivalve zone is positively re-lated with nutrients such as NO_(3)^(-)-N and NH_(4)^(+)-N as well as water depth,while the phytoplankton assemblages in the kelp,control,and IMTA zones are associated with NO_(2)^(-)-N,SiO_(3)^(2-)-Si,and *** all observations into consideration together,it can be inferred that IMTA can effectively reduce Chl-a level compared to bivalve monoculture by reducing the ***,the SR,H’,and species composition of phytoplankton are primarily determined by local environment fa

关键词： phytoplankton diversity Sanggou Bay integrated multi-trophic aquaculture

Hexokinase 1 and 2 mediates glucose utilization to regulate the synthesis of kappa casein via ribosome protein subunit 6 kinase 1 in bovine mammary epithelial cells

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动物营养学报 2024年第1期16卷 338-349页

作者： Tianyu Yang Jia Guo Han Song Osmond Datsomor yuhang Chen Maocheng Jiang Kang Zhan Guoqi Zhao Institute of Animal Culture Collection and Application College of Animal Science and Technology Yangzhou University Yangzhou 225009 China

Glucose plays a vital part in milk protein synthesis through the mTOR signaling pathway in bovine mammary epithelial cells (BMEC). The objectives of this study were to determine how glucose affects hexokinase (HK) activity in BMEC and investigate the regulatory effect of HK in kappa casein (CSN3) synthesis via the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway in BMEC. For this,HK1 andHK2 were knocked out in BMEC using the CRISPR/Cas9 system. The gene and protein expression, glucose uptake, and cell proliferation were measured. We found that glucose uptake, cell proliferation,CSN3 gene expression levels, and expression of HK1 and HK2 increased with increasing glucose concentrations. Notably, glucose uptake was significantly reduced inHK2 knockout (HK2KO) BMEC treated with 17.5 mM glucose. Moreover, under the same glucose treatment conditions, the proliferative ability and abundance of CSN3 were significantly diminished in bothHK1 knockout (HK1KO) and HK2KO BMEC compared with that in wild-type BEMC. We further observed that the phosphorylation levels of ribosome protein subunit 6 kinase 1 (S6K1) were reduced in HK1KO and HK2KO BMEC following treatment with 17.5 mM glucose. As expected, the levels of glucose-6-phosphate and the mRNA expression levels of glycolysis-related genes were decreased in both HK1KO and HK2KO BMEC following glucose treatment. These results indicated that the knockout ofHK1 andHK2 inhibited cell proliferation and CSN3 expression in BMEC under glucose treatment, which may be associated with the inactivation of the S6K1 and inhibition of *** The PDF

关键词： Glucose Hexokinase Milk protein Mechanistic target of rapamycin complex 1 signaling pathway Bovine mammary epithelial cell Kappa casein

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路网环境下基于双锚点的位置隐私保护方法

计算机应用研究 2020年第11期37卷 3392-3395,3400页

作者：周宇航柳毅广东工业大学计算机学院广州510006

针对SpaceTwice方案中查询结果分布不均衡的缺陷,通过结合路网环境提出一种基于双锚点的位置隐私保护方法。该方法采用分布式系统结构,摆脱了可信第三方的性能瓶颈,用户可根据自身的隐私需求构建匿名区,并以锚点作为查询点进行基于双锚... 详细信息

针对SpaceTwice方案中查询结果分布不均衡的缺陷,通过结合路网环境提出一种基于双锚点的位置隐私保护方法。该方法采用分布式系统结构,摆脱了可信第三方的性能瓶颈,用户可根据自身的隐私需求构建匿名区,并以锚点作为查询点进行基于双锚点的增量近邻查询,使查询结果分布更均衡,提高查询准确率。通过在不同路网环境下对该方法进行大量实验结果表明,该方法在保证查询准确率的同时提高了查询效率。

关键词： k-匿名锚点路网环境基于双锚点的增量近邻查询

Periplaneta americana extract promotes infectious diabetic ulcers wound healing by downregulation of LINC01133/SLAMF9

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Chinese Journal of Natural Medicines 2024年第7期22卷 608-618页

作者： YANG yuhang HUANG Jun LI Xintian LIN Renjing WANG Xiaoyan XIAO Ge ZENG Juanni WANG Zhenquan Department of Anorectal Disease 1 The Second Affiliated Hospital of Hunan University of Traditional Chinese MedicineChangsha 410005China Department of Anorectal Disease 3 The Second Affiliated Hospital of Hunan University of Traditional Chinese MedicineChangsha 410005China Laboratory of Vascular Biology and Translational Medicine Medical SchoolHunan University of Chinese Medicine/Hunan Provincial Key Laboratory of Vascular Biology and Translational MedicineChangsha 410208China

Wound healing in diabetic ulcers remains a significant clinical challenge,primarily due to bacterial infection and impaired *** americana extract(PAE)has been widely used to treat diabetic wounds,yet its underlying mechanisms are not fully *** study aimed to elucidate these mechanisms by analyzing long non-coding RNA(lncRNA)expressions in the wound tissues from diabetic anal fistula patients treated with or without PAE,using high-throughput *** blood monocytes from patients were differentiated into M0 macrophages with human macrophage colony-stimulating factor(hMCSF)and subsequently polarized into M1 macrophages with *** results indicated that LINC01133 and SLAMF9 were downregulated in wound tissues of patients treated with ***,PAE suppressed M1 macrophage polarization and enhanced human umbilical vein endothelial cell(HUVEC)proliferation,migration,and *** effects were diminished when LINC01133 or SLAMF9 were ***,LINC01133 was shown to upregulate SLAMF9 through interaction with *** of SLAMF9 reversed the effects of LINC01133 silencing on macrophage polarization and HUVEC *** conclusion,PAE facilitates the healing of infected diabetic ulcers by downregulating the LINC01133/SLAMF9 pathway.

关键词： Periplaneta americana extract Infectious diabetic ulcers M1 macrophage polarization LINC01133 SLAMF9

Accurate cell type annotation for single-cell chromatin accessibility data via contrastive learning and reference guidance

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Quantitative Biology 2024年第1期12卷 85-99页

作者： Siyu Li Songming Tang Yunchang Wang Sijie Li yuhang Jia Shengquan Chen School of Statistics and Data Science Nankai UniversityTianjinChina School of Mathematical Sciences and LPMC Nankai UniversityTianjinChina

Recent advances in single-cell chromatin accessibility sequencing(scCAS)technologies have resulted in new insights into the characterization of epigenomic heterogeneity and have increased the need for automatic cell type ***,existing automatic annotation methods for scCAS data fail to incorporate the reference data and neglect novel cell types,which only exist in a test ***,we propose RAINBOW,a reference-guided automatic annotation method based on the contrastive learning framework,which is capable of effectively identifying novel cell types in a test *** utilizing contrastive learning and incorporating reference data,RAINBOW can effectively characterize the heterogeneity of cell types,thereby facilitating more accurate *** extensive experiments on multiple scCAS datasets,we show the advantages of RAINBOW over state-of-the-art methods in known and novel cell type *** also verify the effectiveness of incorporating reference data during the training *** addition,we demonstrate the robustness of RAINBOW to data sparsity and number of cell ***,RAINBOW provides superior performance in newly sequenced data and can reveal biological implication in downstream *** the results demonstrate the superior performance of RAINBOW in cell type annotation for scCAS *** anticipate that RAINBOW will offer essential guidance and great assistance in scCAS data *** source codes are available at the GitHub website(BioX-NKU/RAINBOW).

关键词： cell type annotation chromatin accessibility novel type reference-guided single-cell