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Cellular & Molecular Immunology 2008年第6期5卷 407-415页

作者： Khawaja Ashfaque Ahmed yufeng xie Xueshu Zhang Jim Xiang Research Unit Saskatchewan Cancer Agency Departments of OncologyMicrobiology and Immunology College of Medicine University ofSaskatchewan 20 Campus Drive Saskatoon Saskatchewan S7N 4H4Canada Ahmed and xie made the same contribution to the study

CD4＋ helper T （Th） cells play pivotal roles in induction of CD8＋ CTL immunity. However, the mechanism of CD4＋ T cell help delivery to CD8＋ T cells in vivo is still elusive. In this study, we used ovalbumin （OVA）-pulsed dendritic cells （DCovA） to activate OT-II mouse CD4＋ T cells, and then studied the help effect of these CD4＋ T cells on CD8＋ cytotoxic T lymphocyte （CTL） responses＋ We also examined CTL mediated islet β cell destruction which leaded to diabetes in wild-type C57BL/6 mice and transgenic rat insulin promoter （RIP）-mOVA mice expressing β cell antigen OVA with self OVA-specific tolerance, respectively. In adoptive transfer experiments, we demonstrated that help, in the form of peptide/major histocompatibility complex （pMHC） I acquired from DCovA by DCovA activation, was required for induction of OVA-specific CTL responses in C57BL/6 mice. However, in combination ＋＋＋ with TCR transgenlc OT-I mouse CD8 T ceils, the tolerogenic dosage of CD4＋ Th cells with acquired pMHC I, but not CD4＋（Kb-/-） Th cells without acquired pMHC I were able to cause diabetes in 8/10 （80%） RIP-mOVA mice. This study thus expands the current knowledge in T cell-mediated autoimmunity and provides insight into the nature of CD4＋ T cell-mediated help in CD8＋ CTL induction. Cellular ＆ Molecular Immunology. 2008;5（6）：407-415.

关键词： CD4+ Th pMHC 1 dendritic cell membrane acquisition diabetes

Calcium-mediated paired pulse depression in juvenile rat dorsal striatum

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Neural Regeneration Research 2012年第10期7卷 772-777页

作者： yufeng xie Michael F. Jackson John F. MacDonald Robarts Research Institute University of Western Ontario London Ontario Canada Department of Physiology and Pharmacology University of Western Ontario London Ontario Canada Department of Anatomy and Cell Biology University of Westem Ontario London Ontario Canada

As the major division of the basal ganglia, neostriatum forms mutual connections with multiple brain areas and is critically involved in motor control and learning/memory. Long-term synaptic plasticity has been widely studied in different species recently. However, there are rare reports about the short-term synaptic plasticity in neostratium. In the present study, using field excitatory postsynaptic potentials recording, we reported one form of short-term synaptic plasticity that is paired pulse depression in juvenile rat dorsal striatum slices induced by stimuli of the white matter. The field excitatory postsynaptic potentials could be abolished by α-amino-3-hydroxy-5-methylizoxazole-4-propionic acid receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione, but not by gamma-aminobutyric acid type A receptor antagonist bicuculline or dopamine D1 receptor antagonist SKF-81297. The paired pulse depression in the corticostratial pathway was different from paired pulse facilitation in the hippocampal CA1 synapse. In addition, the paired pulse depression was not affected by bath application of gamma-aminobutyric acid type A receptor antagonist or dopamine D1 receptor antagonist. However, low calcium and high magnesium could attenuate the paired pulse depression. These findings suggest a more complicated plasticity form in the dorsal striatum of juvenile rats that is different from that in the hippocampus, which is related with extracellular calcium.

关键词： paired pulses depression dorsal striatum calcium juvenile rats

A novel T cell-based vaccine capable of stimulating long-term functional CTL memory against B 16 melanoma via CD40L signaling

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Cellular & Molecular Immunology 2013年第1期10卷 72-77页

作者： yufeng xie Lu Wang Andrew Freywald Mabood Qureshi Yue Chen Jim Xiang Saskatchewan Cancer Agency Department of Oncology University of Saskatchewan Saskatoon Saskatchewan Canada Department of Pathology University ofSaskatchewan Saskatoon Saskatchewan Canada and Department of Epidemiology and Community Medicine University of Ottawa Ottawa Ont. Canada xie and Wang contributed equally to this study.

The ultimate goal of antitumor vaccines is to develop memory CD8＋ cytotoxic T lymphocytes （CTLs）, which are critical mediators of antitumor immunity. We previously demonstrated that the ovalbumin （OVA）-specific CD4＋ T cell-based （OVA-TExo） vaccine generated using OVA-pulsed dendritic cell （DCovA）-released exosomes （EXOovA） stimulate CTL responses via IL-2 and costimulatory CD80 signaling. To assess the potential involvement of other costimulatory pathways and to define the key constituent of costimulation for memory CTL development, we first immunized wild-type （WT） C57BIJ6 and gene-knockout mice with WT CD4~ OVA-TExo cells or OVA-TExo cells with various molecular deficiencies. We then assessed OVA-specific primary and recall CTL responses using PE-H-2Kb/ 0VA257_264 tetramer and FITC-anti-CD8 antibody staining by flow cytometry. We also examined antitumor immunity against the OVA-expressing B16 melanoma cell line BL6-1OovA. We demonstrated that CD4＋ OVA-TExo cells stimulated more efficient CTL responses compared to DCovA. By assessing primary and recall CTL responses in mice immunized with OVA-TExo or with OVA-TExo lacking the costimulatory molecules CD4OL, 4-1BBL or OX4OL, we demonstrated that these costimulatory signals are dispensable for CTL priming by OVA-TExo. Interestingly, CD4OL, but not 4-1BBL or OX4OL, plays a crucial role in the development of functional memory CTLs against BL6-1OovA tumors. Overall, this work suggests that a novel CD4＋ T cell-based vaccine that is capable of stimulating long-term functional CTL memory via CD40L signaling may represent a novel, efficient approach to antitumor vaccination.

关键词： antitumor immunity CD4OL memory CTL T cell-based vaccine

CD4^(+) T cell-released exosomes inhibit CD8^(+) cytotoxic T-lymphocyte responses and antitumor immunity

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Cellular & Molecular Immunology 2011年第1期8卷 23-30页

作者： Haifeng Zhang yufeng xie Wei Li Rajni Chibbar Sidong Xiong Jim Xiang Research Unit Health Research DivisionSaskatchewan Cancer AgencyDepartment of Oncology and ImmunologyUniversity of SaskatchewanSaskatoonSask.Canada Research Unit Health Research DivisionSaskatchewan Cancer AgencyDepartment of PathologyUniversity of SaskatchewanSaskatoonSask.Canada Institute of Biology and Medical Sciences Soochow UniversitySuzhouChina

T cells secrete bioactive exosomes(EXO),but the potential immunoregulatory effect of T-cell EXO is largely *** this study,we generated activated ovalbumin(OVA)-specific CD4^(+) T cells in vitro via coculture of OVA-pulsed dendritic cells(DC_(OVA))with naive CD4^(+) T cells derived from OVA-specific T-cell receptor(TCR)transgenic OTII ***4^(+) T-cell EXO were then purified from the CD4^(+) T-cell culture supernatants by differential ***4^(+) T-cell EXO exhibited the‘saucer’shape that is characteristic of EXO with a diameter between 50 and 100 nm,as assessed by electron microscopy,and contained the EXO-associated proteins LAMP-1,TCR and lymphocyte function associated antigen-1(LFA-1),as determined by western *** cytometric analysis showed that CD4^(+) T-cell EXO expressed CD4^(+) T-cell markers(CD4,TCR,LFA-1,CD25 and Fas ligand),but to a lesser extent than CD4^(+) T *** demonstrated that DC_(OVA) took up CD4^(+) T-cell EXO via peptide/major histocompatibility complex(pMHC)II/TCR and CD54/LFA-1 ***-specific CD4^(+) T-cell EXO from OTII mice,but not ConA-stimulated polyclonal CD4^(+) T-cell EXO from wild-type C57BL/6 mice inhibited DC_(OVA)-stimulated in vitro CD4^(+) T-cell proliferation and in vivo CD81 cytotoxic T lymphocyte(CTL)responses and antitumor immunity against OVA-expressing B16 melanoma BL6-10OVA *** addition,EXO derived from a T-cell hybridoma cell line,MF72.2D9,expressing an OVA-specific CD4^(+) TCR,had a similar inhibitory effect as OTII CD4^(+) T-cell EXO on CTL-mediated antitumor *** together,our data indicate that antigen-specific T-cell EXO may serve as a new type of immunosuppressive reagent for use in transplant rejection and treatment of autoimmune diseases.

关键词： antitumor immunity CTL T-cell exosome

Cuprorivaite/hardystonite/alginate composite hydrogel with thermionic effect for the treatment of periimplant lesion

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Regenerative Biomaterials 2024年第5期3卷 90-105页

作者： Yiru Xia Zhaowenbin Zhang Kecong Zhou Zhikai Lin Rong Shu Yuze Xu Zhen Zeng Jiang Chang yufeng xie Department of Periodontology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina College of Stomatology Shanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai Research Institute of StomatologyShanghaiChina Joint Centre of Translational Medicine the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Zhejiang Engineering Research Center for Tissue Repair Materials Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325000China State Key Laboratory of High-Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Key Laboratory of Rehabilitation Medicine in Sichuan Province West China HospitalSichuan UniversityChengduChina Department of Periodontology Shanghai Stomatological Hospital&School of StomatologyFudan UniversityShanghaiChina Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan UniversityShanghaiChina

Peri-implant lesion is a grave condition afflicting numerous individuals with dental *** results from persistent periodontal bacteria accumulation causing inflammation around the implant site,which can primarily lead to implant loosening and ultimately the implant ***-stage peri-implant lesions exhibit symptoms akin to gum disease,including swelling,redness and bleeding of the gums surrounding the *** signs indicate infection and inflammation of the peri-implant tissues,which may result in bone loss and implant *** address this problem,a thermionic strategy was applied by designing a cuprorivaite–hardystonite bioceramic/alginate composite hydrogel with photothermal and Cu/Zn/Si multiple ions releasing *** innovative approach creates a thermionic effect by the release of bioactive ions(Cu^(2+)and Zn^(2+)and SiO_(3)^(2−))from the composite hydrogel and the mild heat environment though the photothermal effect of the composite hydrogel induced by near-infrared light *** most distinctive advantage of this thermionic effect is to substantially eliminate periodontal pathogenic bacteria and inhibit inflammation,while simultaneously enhance peri-implant *** unique attribute renders the use of this composite hydrogel highly effective in significantly improving the survival rate of implants after intervention in peri-implant lesions,which is a clinical challenge in *** study reveals application potential of a new biomaterial-based approach for peri-implant lesion,as it not only eliminates the infection and inflammation,but also enhances the osteointegration of the dental implant,which provides theoretical insights and practical guidance to prevent and manage early-stage peri-implant lesion using bioactive functional materials.

关键词： bioceramics composite hydrogel thermionic effect peri-implant lesion

Construction of chiral through-space luminophores via symmetry breaking triggered by sequenced chlorination

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Science China Chemistry 2023年第7期66卷 2083-2090页

作者： yufeng xie Huaying Liu Zhongliang Li Xin Zhang Lijuan Song Kang Zhou Feng Yuan Hengzhi You Engui Zhao Zikai He School of Science Harbin Institute of TechnologyShenzhen 518055China School of Chemical Engineering and Technology Harbin Institute of Technology HarbinHarbin 150001China Academy for Advanced Interdisciplinary Studies and Department of Chemistry Southern University of Science and TechnologyShenzhen 518055China Shenzhen Bay Laboratory Shenzhen 518055China Hoffmann Institute of Advanced Materials Shenzhen PolytechnicShenzhen 518055China

The construction of molecular chirality is crucial for exploring novel luminophores with chiroptical *** asymmetric synthesis of chiral center or axial is not powerful enough on through-space *** methodologies for breaking molecular symmetry could be promising but remain less ***,we report a novel methodology for constructing chiral through-space luminophores via simple chlorination on bridged carbazole *** chlorination breaks the molecular symmetry and thus results in molecular chirality by eliminating the mirror plane or rotating ***,continuous multiple chlorinations can rebuild and break the symmetry of the skeleton in *** chiral and achiral isomeric analogues are synthesized and characterized with impressive chiroptical *** of chiral high performance liquid chromatography(HPLC),single-crystal X-ray diffraction,kinetic racemization,and chiroptical property investigation demonstrate the effectiveness of our rational design *** provides a feasible methodology for exploring novel chiral luminescent materials based on versatile though-space skeletons.

关键词： symmetry breaking chiral through-space luminophore circularly-polarized luminescence kinetic racemization

Structural plasticity of human leptin binding to its receptor LepR

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hLife 2023年第2期1卷 115-123页

作者： yufeng xie Xiaoxiong Li Jianxun Qi Guijun Shang Defen Lu George Fu Gao CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina Department of Basic Medical Sciences School of MedicineTsinghua UniversityBeijingChina Shanxi Academy of Advanced Research and Innovation ShanxiChina Shanxi Provincial Key Laboratory for Major Infectious Disease Response ShanxiChina College of Life Sciences Shanxi Agricultural UniversityShanxiChina

Leptin receptor(LepR)signaling plays an essential role in balancing food intake and energy *** architec-ture of LepR signaling assembly is critical for its *** this study,we determined the structures of three distinct conformations of human leptin–LepR using cryo-electron microscopy at resolutions of 3.88,3.77,and 3.58Å.Both 2:2 and 3:3 stoichiometric assemblies were observed,and the complexes exhibited asymmetric open ***-tin undergoes substantial rearrangement of its flexible regions to accommodate binding to *** assembled leptin–LepR complexes connect through a“hand-in-hand”*** open,interlocked 3:3 trimeric assembly results from the engagement of a third leptin–LepR heterodimer with a 2:2 *** asymmetric geometry of LepR is substantially distinct from that of other gp130 cytokine homologs,and that may be due to the twisted and rigid interface between the D3 and D4 *** results highlight the distinct engagement of leptin with LepR and provide important insights into the structural plasticity of LepR-signaling assemblies.

关键词： LepR leptin signaling cryo-electron microscopy(cryo-EM)

3WF-1300型悬挂式喷杆喷雾机的设计与试验

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农机化研究 2025年第3期47卷 77-81页

作者：李存斌梁玉成张庆柱陈宝昌谢宇峰吕海杰宋秋梅黑龙江省农业机械工程科学研究院哈尔滨150081

针对我国目前大田喷雾机仍以中小型机具为主,产品技术含量相对较低,使用可靠性差,施药过程存在雾滴飘移、喷洒不均匀、农药有效利用率低等问题,在分析悬挂式喷杆喷雾机工作原理的基础上设计并研制了一种高效、安全的3WF-1300型悬挂式喷... 详细信息

针对我国目前大田喷雾机仍以中小型机具为主,产品技术含量相对较低,使用可靠性差,施药过程存在雾滴飘移、喷洒不均匀、农药有效利用率低等问题,在分析悬挂式喷杆喷雾机工作原理的基础上设计并研制了一种高效、安全的3WF-1300型悬挂式喷杆喷雾机。该机配套动力为88.2~117.6 kW,药箱容量1 300 L,喷幅18 m,可液压控制喷杆折叠、展开和升降,有效降低了驾驶人员的操控难度,能够实现舒适、精准、高效的农药喷施作业,提高了农药有效利用率、降低了农药施用量、减少了环境污染,极大地保护了操控人员的身体健康。田间试验结果表明:喷雾机结构参数设计合理,喷杆的折叠、展开和升降过程平稳顺滑,无卡顿现象;喷雾均匀,作业效率高,达到了设计指标的要求;田间试验中,沿喷杆方向喷雾分布变异系数为5.3%,结果优于国家标准。

关键词：喷杆式喷雾机折叠喷杆悬挂机构升降机构

维普期刊数据库博看期刊

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百年《诗经》研究献疑

文艺理论研究 2017年第4期37卷 89-99页

作者：解玉峰南京大学文学院

胡适等近代学者勇于打破传统的禁锢,为近百年来的《诗经》研究做出许多了开创性贡献,其影响至今犹存。自新文化运动到现在,人们对相关《诗经》的基本问题也达成一些共识:如《诗经》是中国最早的诗歌总集、《诗经》皆为"乐歌"、《诗经》... 详细信息

胡适等近代学者勇于打破传统的禁锢,为近百年来的《诗经》研究做出许多了开创性贡献,其影响至今犹存。自新文化运动到现在,人们对相关《诗经》的基本问题也达成一些共识:如《诗经》是中国最早的诗歌总集、《诗经》皆为"乐歌"、《诗经》分"风""雅""颂"三类等。然而相关《诗经》这些基本问题,或者还需要我们重新面对和思考,有些学术共识也可能存在严重的误解。今人理解《诗经》当然离不开前人的启发和引导,但我们也可能不自觉地被一些前人所误导,这些"前人"既包括汉唐人或宋元人、明清人,也包括胡适之、顾颉刚等近代人。只有不断拨开重重迷障,才能不断接近历史真相,《诗经》真相的研究可能永远是在路上。

关键词：《诗经》诗歌总集乐歌 “风”“雅”“颂”

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“曲”变为“词”:长短句韵文之演进

文艺理论研究 2014年第2期34卷 151-162页

作者：解玉峰南京大学文学院

中国古代歌唱之歌辞,自其文体形式看,可分齐言、杂言(长短句)两类。初唐"沈宋体"的出现标志着齐言韵文格律化的完成,而杂言(长短句)韵文的格律化中晚唐以后也渐次展开。如果我们把未格律化、规范化的长短句界定为"曲"、格律化的长短句... 详细信息

中国古代歌唱之歌辞,自其文体形式看,可分齐言、杂言(长短句)两类。初唐"沈宋体"的出现标志着齐言韵文格律化的完成,而杂言(长短句)韵文的格律化中晚唐以后也渐次展开。如果我们把未格律化、规范化的长短句界定为"曲"、格律化的长短句界定为"词",那么千余年来长短句韵文的演进实即"曲"不断变为"词"的过程。数百年来,"诗变为词"、"词变为曲"乃是人们述及诗词曲嬗变的流行性观念,对此我们自应抱"了解之同情",但不必以古人之是非为是非。

关键词：曲变为词词变为曲宋词元曲南北曲