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China CDC weekly 2024年第27期6卷 658-664,I0001-I0004页

作者： Jing Han Jian Li Yuhua Shi Chunpeng Zang Zhimin Yang Yu Han Yanling Ma Qiongli Duan Yuhua Hu Zikuang Bai Cuiyun Yang Gabriella Bulloch Yurong Mao Houlin Tang Zunyou Wu yufen liu National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Yunnan Provincial Center for Disease Control and Prevention Kunming CityYunnan ProvinceChina Honghe Prefectural Center for Disease Control and Prevention Honghe CityYunnan ProvinceChina Wenshan Prefectural Center for Disease Control and Prevention Wenshan CityYunnan ProvinceChina Zhaotong Prefectural Center for Disease Control and Prevention Zhaotong CityYunnan ProvinceChina Lincang Prefectural Center for Disease Control and Prevention Lincang CityYunnan ProvinceChina Faculty of Medicine Dentistry and Health SciencesUniversity of MelbourneMelbourneVICAustralia

Summary What is already known about this topic?HIV transmission among serodiscordant couples remains a persistent issue in ***,the practice of combining counseling with antiretroviral therapies(ART)to enhance ART adhe... 详细信息

关键词： China ant discord

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工业用寡聚酶的改造与设计研究进展

中国生物工程杂志 2024年第7期44卷 89-99页

作者：刘玉芬刘森湖北工业大学工业发酵省部共建协同创新中心武汉430068 湖北微生元生物科技有限公司鄂州436006 湖北工业大学发酵工程教育部重点实验室武汉430068

在自然界中,绝大多数酶具有寡聚特性。寡聚酶在工业上具有重要的价值,它能够提高反应速度和效率,降低生产成本,减少废物产生,扩大反应底物范围,提高产品质量。这些优势使得寡聚酶成为工业生产中不可或缺的催化剂。但是,寡聚酶在催化过程... 详细信息

在自然界中,绝大多数酶具有寡聚特性。寡聚酶在工业上具有重要的价值,它能够提高反应速度和效率,降低生产成本,减少废物产生,扩大反应底物范围,提高产品质量。这些优势使得寡聚酶成为工业生产中不可或缺的催化剂。但是,寡聚酶在催化过程中,亚基解离可能导致酶活性减弱,这是其工业化应用的主要限制因素之一。因此,提升工业用寡聚酶的催化稳定性是当前生物催化与转化研究领域的重要课题。近年来,研究人员致力于通过优化手段提高寡聚酶的稳定性和催化活性以满足工业应用的需求。综述工业用寡聚酶的普遍性、寡聚化调节过程、功能影响以及分子改造情况,同时对未来工业化利用寡聚酶的发展前景进行展望,以期实现对寡聚酶催化和反应性能的精确调控,为高效工业用酶战略的发展提供理论指导。

关键词：工业用寡聚酶稳定性催化活性分子改造

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工业萘氧化制苯酐催化剂性能研究

石油化工 2024年第5期53卷 643-648页

作者：袁滨安欣刘玉芬中石化(北京)化工研究院有限公司北京100013

开发了一种工业萘氧化制苯酐催化剂(简称萘法苯酐催化剂),并进行了工业生产量级制备。采用单管模试装置对催化剂的反应工艺参数进行了研究。实验结果表明,开发的萘法苯酐催化剂在单管风量4.0 m^(3)/h、熔盐温度340~350℃时,萘负荷达75~8... 详细信息

开发了一种工业萘氧化制苯酐催化剂(简称萘法苯酐催化剂),并进行了工业生产量级制备。采用单管模试装置对催化剂的反应工艺参数进行了研究。实验结果表明,开发的萘法苯酐催化剂在单管风量4.0 m^(3)/h、熔盐温度340~350℃时,萘负荷达75~85 g/m^(3),反应器出口苯酐收率为102%~104%(w),产物中萘醌收率不高于0.5%(w)。诱导期开停车实验结果表明,催化剂具有优异的抗频繁开停车能力。催化剂老化实验结果表明,开发的工业萘法苯酐催化剂具有良好的抗飞温稳定性。此外,重复开停车过程中萘法苯酐催化剂性能基本不变,稳定性好。

关键词：萘苯酐催化剂稳定性开停车

不同轧制变形量下7050铝合金的轧制变形与组织性能

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锻压技术 2024年第8期49卷 80-87页

作者：周晓平田壮壮刘玉芬郑州西亚斯学院电信与智能制造学院河南郑州450000 河南科技大学材料科学与工程学院河南洛阳471000

采用X射线衍射分析仪、光学显微镜、扫描电子显微镜和拉伸试验机等设备,研究了轧制变形量(25%~75%)对7050铝合金的物相组成、显微组织和力学性能的影响。结果表明,挤压态和轧制变形量为25%~75%的7050铝合金均主要由α-Al相、MgZn_(2)相... 详细信息

采用X射线衍射分析仪、光学显微镜、扫描电子显微镜和拉伸试验机等设备,研究了轧制变形量(25%~75%)对7050铝合金的物相组成、显微组织和力学性能的影响。结果表明,挤压态和轧制变形量为25%~75%的7050铝合金均主要由α-Al相、MgZn_(2)相和Al_(2)MgCu相组成;随着轧制变形量从25%增加至75%,7050铝合金在3个方向的显微硬度、抗拉强度和屈服强度均先增大后减小,在轧制变形量为55%时取得硬度最大值,且此时7050铝合金具有较大的断后伸长率,这主要与轧制变形量为55%时,7050铝合金中的再结晶晶粒、MgZn_(2)相和Al_(2)MgCu相较为细小、位错密度较大,以及存在胞状亚结构有关。当轧制变形量增大至75%时,晶粒粗化与长大的同时,基体组织中的位错密度降低,显微硬度、强度会有所减小。

关键词： 7050铝合金挤压态轧制变形量物相组成显微组织力学性能

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等离子物理气相沉积高熵合金涂层及组织性能

电镀与精饰 2024年第3期46卷 75-83页

作者：周晓平许新华刘玉芬郑州西亚斯学院河南郑州450000 河南科技大学材料科学与工程学院河南洛阳471000

采用等离子物理气相沉积的方法在316L不锈钢表面制备了AlCoCrFeNi高熵合金涂层,研究了喷涂距离和电流对高熵合金涂层物相组成、表面形貌、截面形貌、硬度、结合强度和耐磨性的影响。结果表明,不同喷涂距离和电流下,高熵合金涂层都主要由... 详细信息

采用等离子物理气相沉积的方法在316L不锈钢表面制备了AlCoCrFeNi高熵合金涂层,研究了喷涂距离和电流对高熵合金涂层物相组成、表面形貌、截面形貌、硬度、结合强度和耐磨性的影响。结果表明,不同喷涂距离和电流下,高熵合金涂层都主要由BCC、B2和FCC相组成;随着电流或者喷涂距离增加,涂层中BCC平均晶粒尺寸先增后减。当喷涂距离为460 mm时,随着电流从1600A增加至2000A,涂层平均摩擦系数逐渐增大,表面和截面硬度先减后增,涂层结合力和结合强度先增大后减小,涂层的磨损率先增加后减小;当电流为1800A时,随着喷涂距离从420mm增加至500mm,涂层平均摩擦系数逐渐减小,表面硬度先减后增,截面硬度先增后减,涂层结合力和结合强度逐渐增大,涂层的磨损率逐渐减小。高熵合金涂层的磨损率与涂层表面硬度和内聚强度都有一定相关性。

关键词：等离子物理气相沉积高熵合金涂层显微形貌硬度耐磨性

H5亚型禽流感疫苗诱导下鹅TLR4及相关免疫因子表达分析

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广东畜牧兽医科技 2024年第4期49卷 17-23,42页

作者：王权锜许冬梅徐笑邢波建刘玉芬哈尔滨师范大学生命科学与技术学院黑龙江哈尔滨150025 萝北县高级中学黑龙江鹤岗154200 哈尔滨师范大学教师教育学院黑龙江哈尔滨150025 中国共产党齐齐哈尔梅里斯达斡尔族区人才发展与服务中心黑龙江齐齐哈尔161000

进一步明确TLR4及其相关免疫因子在禽类疫苗免疫过程中的表达变化。采用荧光定量RT-PCR方法和免疫组织化学技术,在注射H5亚型禽流感疫苗24 h后,对三花鹅TLR4及相关免疫因子在不同组织中的表达情况进行检测并分析。在H5亚型禽流感疫苗诱... 详细信息

进一步明确TLR4及其相关免疫因子在禽类疫苗免疫过程中的表达变化。采用荧光定量RT-PCR方法和免疫组织化学技术,在注射H5亚型禽流感疫苗24 h后,对三花鹅TLR4及相关免疫因子在不同组织中的表达情况进行检测并分析。在H5亚型禽流感疫苗诱导下,实验组TLR4 mRNA在肝脏和骨髓中显著高于对照组(P<0.05),在脾脏、法氏囊以及胸腺中极显著高于对照组(P<0.01);IL-1β、IFN-α以及IFN-γ在肝脏和胸腺中极显著高于对照组(P<0.01),IL-6和IFN-α在脾脏中与对照组相比为极显著(P<0.01),但IL-1β在脾脏和法氏囊中只是显著高于对照组的水平(P<0.05)。免疫组织化学检测结果显示,TLR4在脾脏和法氏囊中与对照组相比极显著(P<0.01),在肝脏中为显著差异(P<0.05),与转录水平一致。在H5亚型禽流感疫苗诱导下,三花鹅体内能够激活TLR4及其相关免疫因子,但在不同组织中表达具有一定差异性。

关键词： TLR4 细胞因子表达特征三花鹅

HIV Prevention and Health Poverty Alleviation-Liangshan Prefecture,Sichuan Province,China,2017-2020

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China CDC weekly 2021年第48期3卷 1031-1035页

作者： Zhongfu liu Xuefeng Tang yufen liu Linglin Zhang Yihui Yang Yongli Zheng Weixin liu Chunnong Jike Hong Mai Heying Fan Gang Yu Mengjie Han George F.Gao National Center for AIDS/STD Control and Prevention China CDCBeijingChina Sichuan Provincial Center for Disease Control and Prevention ChengduSichuanChina Chengdu Public Health Clinical Center ChengduSichuanChina Sichuan Provincial Maternity and Child Health Care Hospital ChengduSichuanChina Liangshan Prefecture Center for Disease Control and Prevention XichangSichuanChina The First People’s Hospital of Liangshan Prefecture XichangSichuanChina Liangshan Prefecture Maternal&Child Health and Family Planning Service Center XichangSichuanChina Chinese Center for Disease Control and Prevention BeijingChina

BACKGROUND Liangshan,officially the Liangshan Yi Autonomous Prefecture,is an autonomous prefecture occupying much of the southern portion of Sichuan *** has been a remote and deeply impoverished area and also has the largest population of ethnic Yi people in China(1).For many years,due to geographical environment conditions,traffic conditions,and other historic restrictions,the human immunodeficiency virus(HIV)epidemic in Liangshan Prefecture has become the focus of HIV prevention and control in China since the first acquired immunodeficiency syndrome(AIDS)case was reported in this area in 1995(2-3).

关键词： Province. Prefecture prevention

Direct Synthesis of α-Aminophosphonates from Amines,Alcohols,and White Phosphorus

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CCS Chemistry 2024年第1期6卷 91-99页

作者： Mengpei Bai Yinwei Cao Junwei Huang Yan liu Guo Tang yufen Zhao Department of Chemistry and Key Laboratory for Chemical Biology of Fujian Province College of Chemistry and Chemical EngineeringXiamen UniversityXiamenFujian 361005

Developing practical strategies for the synthesis of organophosphorus compounds(OPCs)from white phosphorus(P_(4))without the use of Cl_(2) and PCl_(3) remains a significant *** first multicomponent oxidative α-phosphonylation of amines with P_(4) and alcohols has been *** the use of copper(Ⅱ)as the catalyst and air as the safe oxidant,structurally sophisticated α-aminophosphonates have been prepared in high ***,this method is also suitable for selective construction of P-O-P *** reaction is characterized by a complete conversion of P_(4).The activation of P_(4) with transition metals often leads to formation of complexes[M_(x)P_(y)]_(n) associated with the deactivation of transition *** breakthrough showcases the potential of transition-metal-catalyzed reactions in elemental phosphorus chemistry.

关键词： white phosphorus P-C bonds dialkyl phosphites aminophosphonates pyrophosphates

Study on goose TLR4、15 and 21 induced by LPS

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Study on goose TLR4、15 and 21 induced by LPS

Macrocyclic trichothecenes from Myrothecium verrucaria PA 57 and their cytotoxic activity

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Xing Bojian Xuxiao Bian ruofei liu yufen Harbin Normal University

Toll-like receptors(TLRs) are a class of important pattern recognition molecules, which play an important role in natural immunity and also mediate adaptive immune response. In this study, the extracellular domain sequences of TLR4, 15 and 21 genes of Northeast domestic Goose(Anser Cygnoides) were cloned by RT-PCR and analyzed by bioinformatics. At the same time, some extracellular domain sequences were inserted into prokaryotic expression vector pET32 a and optimized expression in *** BL21(DE3). The fusion protein was detected by SDS-PAGE and were used to immunize rabbits to prepare polyclonal antibodies. The level of antibody was detected by ELISA and the recombinant protein was identified by Western Blot. Secondly, the inflammation model of Northeast domestic goose induced by LPS was established. Real-time PCR and immunohistochemical techniques were used to detect the expression of TLR4, 15 and 21 in goose tissues. The results showed that TLR4, 15 and 21 part had 8, 9 and 9 leucine enrichment regions, the size of recombinant protein was 40 ku, 43 Ku and 42 ku, respectively, and the titer of antibody was above 1:20000. Under LPS induction, TLR4, 15 and 21 were the highest expression in bursa of Fabricius, followed by thymus and spleen. IL-1β, IL-6, IFN-α and IFN-γ were up-regulated in immune organs. It provided a theoretical basis for the study of natural immune regulation in poultry, and also provided a basis for the development of natural adjuvants.

关键词： Anser Cygnoides Toll-like receptors Prokaryotic expression tissue distribution

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Chinese Journal of Natural Medicines 2024年第9期22卷 854-863页

作者： MO Jisong TAN yufen AI Wenjing LI Yunyun YUAN Yiyun JIANG Yueping XU Kangping TAN Guishan WANG Wenxuan LI Jing liu Shao Department of Pharmacy National Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South UniversityChangsha 410008China Xiangya School of Pharmaceutical Sciences Central South UniversityChangsha 410008China

Four novel macrocyclic trichothecenes,termed mytoxins D-G(1-4),along with four known analogs(5-8),were isolated from the ethyl acetate extract of fermented rice inoculated with the fungus Myrothecium verrucaria *** compound features a tricyclic 12,13-epoxytrichothec-9-ene(EPT)***,mytoxin G(4)represents the first instance of a macrocyclic trichothecene incorporating a glucosyl unit within the trichothecene *** structures of the newly identified compounds were elucidated through comprehensive spectroscopic analysis combined with quantum chemical *** isolated compounds demonstrated cytotoxic activity against the CAL27 and HCT116 cell lines,which are models for human oral squamous cell carcinoma and colorectal cancer,***,mytoxin D(1)and mytoxin F(3)exhibited pronounced cytotoxic effects against both cancer cell lines,with IC_(50)values ranging from 3 to 6 nmol·L^(-1).Moreover,compounds 1 and 3 were found to induce apoptosis in HCT116 cells by activating caspase-3.

关键词： Macrocyclic trichothecenes Myrothecium verrucaria Cytotoxicity Secondary metabolite Soil fungi