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Effect of ciliary neurotrophic factor on bcl-2 and c-jun gene and protein expression in cultured retinal nerve cells

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Neural Regeneration Research 2010年第9期5卷 662-667页

作者： Xiang Zhang Xiang Lei yueyue liu Genlin Li Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Sciences Key LaboratoryBeijing 100730 China

BACKGROUND：In various retinal neurodegenerative animal models,ciliary neurotrophic factor （CNTF） exhibits prominent neuroprotective effects on retinal nerve ***-2 is an anti-apoptotic protein.c-Jun is upregulated and phosphorylated in the activated c-Jun N-terminal kinase pathway,which subsequently mediates ***,the effect of CNTF on Bcl-2 and c-Jun expression in retinal nerve cells remains ***：To determine the dynamic changes in retinal nerve cell apoptosis,as well as bcl-2 and c-jun gene and protein expression,following a single dose of CNTF in a short period of ***,TIME AND SETTING：A single-blind,randomized,controlled,in vitro experiment was performed at the Central Laboratory of Beijing Tongren Hospital from May 2008 to April ***：Neonatal bovine retinal nerve cells （Chinese Holstein）,recombinant human CNTF （PeproTech,Rocky Hill,NJ,USA）,rabbit polyclonal anti-Bcl-2 and c-Jun antibodies （Abeam,Cambridge,UK）,fluorescein isothiocyanate-conjugated annexin V/propidium iodide kit （BioVision,Mountain View,CA,USA）,real time polymerase chain reaction instrument （ABI,Foster City,CA,USA）,and flow cytometer （BD FACSCalibur,Franklin Lakes,NJ,USA）.METHODS：Neonatal bovine retinal cells from passage 2 were cultured for 3 days and incubated with,or without,50 ng/mL CNTF （control）.MAIN OUTCOME MEASURES：Cell apoptosis was detected via Annexin V-FITC/PI double-staining and flow ***-2 and c-jun mRNA and protein expression were detected by quantitative real time polymerase chain reaction and western blot ***：The proportion of late-stage apoptotic cells was significantly decreased at 2,4,and 6 days after CNTF treatment compared with the control group （P 〈 0.01）.CNTF did not alter bcl-2 mRNA expression at the three time points,but significantly increased Bcl-2 protein expression at 2 and 4 days （P 〈 0.01）.c-jun mRNA expression was significantly decreased 4 days after CNTF treatment （P〈 0.01）.In addition,c-Jun pro

关键词： ciliary neurotrophic factor c-jun bcl-2 apoptosis nerve cells retina neural factor neural regeneration

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Alternative splicing of inner-ear-expressed genes

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Frontiers of Medicine 2016年第3期10卷 250-257页

作者： Yanfei Wang yueyue liu Hongyun Nie Xin Ma Zhigang Xu Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology School of Life Sciences Shandong University Jinan 250100 China School of Control Science and Engineering Shandong University Jinan 250061 China

Alternative splicing plays a fundamental role in the development and physiological function of the inner ear. Inner-ear-specific gene splicing is necessary to establish the identity and maintain the function of the inner ear. For example, exon 68 of Cadherin 23 （Cdh23） gene is subject to inner-ear-specific alternative splicing, and as a result, Cdh23（＋ 68） is only expressed in inner ear hair cells. Alternative splicing along the tonotopic axis of the cochlea contributes to frequency tuning, particularly in lower vertebrates, such as chickens and turtles. Differential splicing of Kcnmal, which encodes for the a subunit of the Ca2＋-activated K~ channel （BK channel）, has been suggested to affect the channel gating properties and is important for frequency tuning. Consequently, deficits in alternative splicing have been shown to cause hearing loss, as we can observe in Bronx Waltzer Cov） mice and Sfswap mutant mice. Despite the advances in this field, the regulation of alternative splicing in the inner ear remains elusive. Further investigation is also needed to clarify the mechanism of hearing loss caused by alternative splicing deficits.

关键词： alternative splicing inner ear hearing loss hair cells

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Degradation of tiamulin by a packed bed dielectric barrier plasma combined with TiO_(2)catalyst

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Plasma Science and Technology 2022年第9期24卷 117-126页

作者： Kun YANG Hongwei SHEN yueyue liu Yang liu Pingji GE Dezheng YANG School of Science Shihezi UniversityShihezi 832003People’s Republic of China Key Laboratory of Environmental Monitoring and Pollutant Control of Xinjiang Bingtuan Shihezi UniversityShihezi 832003People’s Republic of China Key Lab of Materials Modification Dalian University of TechnologyMinistry of EducationDalian 116024People’s Republic of China

Recently,a plasma catalyst was employed to efflciently degrade antibiotic residues in the *** this study,the plasma generated in a packed bed dielectric barrier reactor combined with TiO_(2)catalyst is used to degrade the antibiotic tiamulin(TIA)loaded on the surface of simulated soil *** effects of applied voltage,composition of the working gas,gas flow rate and presence or absence of catalyst on the degradation effect were *** was found that plasma and catalyst can produce a synergistic effect under optimal conditions(applied voltage 25 k V,oxygen ratio 1%,gas flow rate 0.6 l min^(-1),treatment time 5 min).The degradation efflciency of the plasma combined with catalyst can reach 78.6%,which is 18.4%higher than that of plasma without *** the applied voltage is 30 k V,the gas flow rate is 1 l min^(-1),the oxygen ratio is 1%and the plasma combined with TiO_(2)catalyst treats the sample for 5 min the degradation efflciency of TIA reached 97%.It can be concluded that a higher applied voltage and longer processing times not only lead to more degradation but also result in a lower energy *** the oxygen ratio and gas flow rate could improve the degradation *** relative distribution and identity of the major TIA degradation product generated was determined by high-performance liquid chromatography–mass spectrometry *** mechanism of TIA removal by plasma and TiO_(2)catalyst was analyzed,and the possible degradation path is discussed.

关键词： packed bed dielectric barrier discharge plasma catalyst tiamulin(TIA)antibiotics degradation degradation mechanism

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Polyphenols and pectin enriched golden kiwifruit (Actinidia chinensis) alleviates high fructose-induced glucolipid disorders and hepatic oxidative damage in rats:in association with improvement of fatty acids metabolism

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Food Science and Human Wellness 2023年第5期12卷 1872-1884页

作者： Aamina Alim Ting Li Tanzeela Nisar Zeshan Ali Daoyuan Ren yueyue liu Xingbin Yang Shaanxi Engineering Laboratory for Food Green Processing and Safety Control and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural ProductsCollege of Food Engineering and Nutritional ScienceShaanxi Normal UniversityXi'an 710119China Faculty of Rehabilitation and Allied Health Sciences Riphah International UniversityLahore 54000Pakistan Department of Life sciences Barani InstitutionSahiwal 57000Pakistan

This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-instigated dyslipidemia, hepatic steatosis, oxidative stress, insulin resistance, and fatty acid metabolism disorders in rats. In general, the above abnormalities were improved after 10 weeks intervention of ACF, ACP, and ACFP. Especially, ACFP considerably ameliorated HF-induced abnormal changes in body weight gain, serum TC, TG, LDL-C and HDL-C levels, as well as serum and hepatic SFAs, MUFAs and PUFAs contents. ACFP also alleviated HF-induced hyperglycemia and hyperinsulinemia, stabilized HF-caused increase in hepatic MDA and serum ALT, AST levels, and restored HF-declined hepatic T-SOD and GSH-Px activities. Besides, histopathology of the liver further endorsed the protective effects of ACFP on hepatocellular injury. Moreover, ACFP increased HF-dropped acetic, propionic and butyric acid levels. Overall, ACFP employs more efficacious protective effects against HF-induced metabolic disorders and liver damage than ACF and ACP. This study delivers a scientific foundation for developing kiwifruit(counting peel)-based dietary supplements for those with glucolipid-metabolic disorders and liver damage.

关键词： Actinidia chinensis peel Lipid homeostasis Oxidative stress Insulin resistance Hepatocellular injury Fatty acid metabolism

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Robust Control Strategy of Gradient Magnetic Drive for Microrobots Based on Extended State Observer

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Cyborg and Bionic Systems 2022年第1期2022卷 210-220页

作者： Jiawei Lu yueyue liu Wentao Huang Kaitao Bi Yixin Zhu Qigao Fan College of Internet of Things Engineering Jiangnan UniversityWuxi 214000China

Microrobots have great application pot ential in the biomedical field,to realize the precision and eficiency of microrobots in vivo is research focus in this *** are accompanied by various disturbances in complex *** disturbances will affect the motion control of microrobots,resulting in the inability of the micromanipulation tasks to be completed *** this end,a robust motion control method is proposed for precise path tracking of microrobots in this *** extended state observer(ESO)is used to estimate the total disturbances and uncertainties of the system.A path tracking controller is designed by combining sliding mode control(SMC)and disturbances compensation,which is used to eliminate the total disturbances of the system and realize the fast and accurate path tracking of ***,the path tracking experiments are implemented in the gradient magnetic field drive *** experimental results show that the mean absolute error of the path tracking for microrobots in a simulated vascular structure is less than 14μm,and the root mean square error is less than 17 um by using the robust control method proposed in this *** with the traditional PID control method,it can better suppress external disturbances and uncertainties of the system and improve the path tracking accuracy of microrobots *** shows stronger anti interference ability and robustness.

关键词： Observer system Strategy

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作为现代性建构手段的现代文学早期英译——以《天下月刊》的《雷雨》译介为例

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四川大学学报（哲学社会科学版） 2023年第2期 177-185,200页

作者：刘月悦中国社会科学院大学文学院北京102488

20世纪20至40年代,在新文学主将们以外国文学的译入作为实现中国文学、思想乃至社会现代化的手段的同时,也有一些中国知识分子,尝试着把刚刚诞生不久的中国现代文学译介到西方。这种实践包含着现代性的努力和期许,却往往为研究者所忽略... 详细信息

20世纪20至40年代,在新文学主将们以外国文学的译入作为实现中国文学、思想乃至社会现代化的手段的同时,也有一些中国知识分子,尝试着把刚刚诞生不久的中国现代文学译介到西方。这种实践包含着现代性的努力和期许,却往往为研究者所忽略。1936至1937年,英文期刊《天下月刊》连载姚莘农翻译的《雷雨》,这是曹禺这部重要剧作的首个英文译本。《天下月刊》从其“阐释现代中国”的办刊理念出发,试图以《雷雨》作为中国现代文学的优秀代表,与世界文学“对话”,从而帮助中国获取现代国际共同体中的成员资格。这种以翻译作为现代性建构手段的诉求,既体现在了《天下月刊》对《雷雨》的评价和定位上,也决定了作为译者和《天下月刊》编辑的姚莘农在翻译中采用了颇受争议的直译策略。《天下月刊》同仁与五四作家们形成了一种“接力”,在创作与翻译的不同领域,共同为中国文学和文化的现代性转型努力。

关键词：《天下月刊》《雷雨》外译现代性

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交游、交融、交流——论中国现代诗的两个早期英译选集

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学术月刊 2023年第1期55卷 164-172页

作者：刘月悦中国社会科学院大学文学院北京102488

哈罗德·阿克顿与陈世骧合作编译的《中国现代诗选》和罗伯特·白英编译的《当代中国诗选》,是最早的两部中国现代新诗英译选集。他们的外国编译者在旅居中国的过程中,认识到西方世界对现代中国尤其是现代中国文学知之甚少,希望通过诗... 详细信息

哈罗德·阿克顿与陈世骧合作编译的《中国现代诗选》和罗伯特·白英编译的《当代中国诗选》,是最早的两部中国现代新诗英译选集。他们的外国编译者在旅居中国的过程中,认识到西方世界对现代中国尤其是现代中国文学知之甚少,希望通过诗歌翻译增进西方对中国的了解,从而开始了对中国现代诗的译介。两本诗集的译源选择,既受到译者在中国交游的影响,也呈现出中西方文学观念的交融与碰撞。两个集子在欧美产生的影响和在中国引起的争议,体现出了译者与批评者之间在传播价值与学术价值之间的不同取舍。本文希望通过两个个案的对比研究,在“同”与“异”之间,还原中国现代诗歌早期英译的历史现场,总结中国文学海外传播的经验和路径。

关键词：《中国现代诗选》《当代中国诗选》英译现代诗

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论谍战英雄传奇的文体创新

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中国文学批评 2023年第3期 146-153,192页

作者：刘月悦中国社会科学院大学文学院北京102488

21世纪以来,谍战题材的小说创作蔚为大观。这类小说鲜明地继承了中国古典小说中英雄传奇一脉的文体特点并加以创新,可称之为“谍战英雄传奇”。在人物塑造上,它在继承古典英雄传奇“异能”书写和“家国”情怀的同时,着力塑造“孤胆英雄... 详细信息

21世纪以来,谍战题材的小说创作蔚为大观。这类小说鲜明地继承了中国古典小说中英雄传奇一脉的文体特点并加以创新,可称之为“谍战英雄传奇”。在人物塑造上,它在继承古典英雄传奇“异能”书写和“家国”情怀的同时,着力塑造“孤胆英雄”式的人物形象,注重凸显“谍”的职业特性,呈现出“尚智”的美学风格。在情节构造上,它融合了公案小说、侠情小说以及西方悬疑小说的诸多资源,又有多方力量的引入、爱情元素与政治元素的嫁接等创新。不过,这一小说类型也逐渐出现程式化的倾向,面对挑战,一方面需要进行思想深度的开掘,另一方面也函待新鲜血液的涌入。

关键词：英雄传奇谍战人物塑造情节构造程式化

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“它是现实世界无限伸长的影子”

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南方文坛 2023年第3期 103-107页

作者：刘月悦中国社会科学院大学文学院

2006年以来,刘亮程以持续的长篇小说创作(《虚土》(2006)、《凿空》(2010)、《捎话》(2018))建立起一个诡异奇崛的“刘亮程宇宙”(2)。立足于新疆这一偏居一隅又广袤无垠的土地,刘亮程从黄土梁出发,不断地丰富着叙事的时间和空间,这片... 详细信息

2006年以来,刘亮程以持续的长篇小说创作(《虚土》(2006)、《凿空》(2010)、《捎话》(2018))建立起一个诡异奇崛的“刘亮程宇宙”(2)。立足于新疆这一偏居一隅又广袤无垠的土地,刘亮程从黄土梁出发,不断地丰富着叙事的时间和空间,这片土地上的历史、传说,都以非常具有刘亮程特色的诗性语言呈现出来。于是,这位原本以乡土散文蜚声于文坛的“扛着铁锨在田野上闲荡的农民”(3),逐渐成长为手持卷帙讲述异域故事的说书人,而2022年的长篇小说《本巴》,则是“刘亮程宇宙”的又一次生长和拓展。

关键词：长篇小说创作刘亮程诗性语言《凿空》偏居一隅《虚土》黄土梁奇崛

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磁性微型机器人神经自适应轨迹跟踪控制

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机器人 2023年第6期45卷 683-690页

作者：刘跃跃王浩羽张加易樊启高江南大学物联网工程学院江苏无锡214122

针对复杂环境和不确定的动态模型问题,提出了一种自适应神经网络控制算法,并且基于李雅普诺夫理论保证了闭环系统的稳定性。相较而言,基于模型的控制策略需要精准地知道微机器人的动力学以及周围环境参数,而本文提出的基于径向基函数神... 详细信息

针对复杂环境和不确定的动态模型问题,提出了一种自适应神经网络控制算法,并且基于李雅普诺夫理论保证了闭环系统的稳定性。相较而言,基于模型的控制策略需要精准地知道微机器人的动力学以及周围环境参数,而本文提出的基于径向基函数神经网络的状态反馈控制策略可以有效地根据状态与期望轨迹在线估计系统模型的不确定项。最后,在所开发的磁场驱动的微型机器人系统上进行了2项实验并进行了对比,以验证所提出的控制器的有效性。结果表明,曲线与直线的轨迹跟踪均方根误差分别达到6.2204像素与6.4279像素,明显优于传统的PID(比例-积分-微分)算法。

关键词：磁性微机器人自适应神经网络参数不确定李雅普诺夫函数轨迹跟踪

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