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Clinical observation of pediatric-type follicular lymphomas in adult:Two case reports

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World Journal of Clinical Cases 2021年第31期9卷 9542-9548页

作者： Yao liu Hui Xing yue-ping liu Department of Pathology The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Pediatric-type follicular lymphoma(PTFL)is a unique pathological type in the 4th edition of hematopoiesis and lymphoid tissue tumor classification revised by World Health *** is unique in clinical practice and seldom seen in *** mainly occurs in the head and neck lymph *** of the cases are short of fever,night sweat,weight loss,and other B symptoms which substitute for lymphadenopathy as the main *** can be disposed of surgical resection and it can achieve long-term tumor-free survival,and it has an excellent *** SUMMARY Two cases of PTFL were reported and their clinicopathological features,differential diagnosis,therapy and prognosis were *** showed graybrown tough texture in general *** histological manifestations of PTFL were similar to that of adult-follicular lymphoma(FL).Under low power microscope,the structure of lymph nodes was destroyed in different degree,the follicles were closely arranged,expanded and irregular,and the mantle zone became thin or *** addition,the“starry sky phenomenon”could be *** high magnification,the follicles were mainly composed of single medium-sized central cells,and some of them mainly consisted of centroblastic cells to characterize scattered chromatin and inconspicuous *** showed the tumor cells expressed CD20,PAX5,CD79a and CD10,BCL6,FOXP-1,which were limited in germinal center;Ki-67 was highly expressed in germinal ***21 and CD23 showed nodular and expanded follicular dendritic *** gene rearrangement was positive for IGH and *** two patients underwent surgical resection with no *** discharge,the two patients with a close review for 18 mo and 5 mo respectively and showed no evidence of *** PTFL in adult is generally supposed to be extremely *** displayed characteristic morphological,immunophenotypic,and molecular biological changes which are a ki

关键词： Pediatric-type follicular lymphoma Adult Clinical pathology Diagnosis Immunophenotype Case report

Root-fed Salicylic Acid in Grape Involves the Response Caused by Aboveground High Temperature

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Journal of Integrative Plant Biology 2008年第6期50卷 761-767页

作者： Hong-Tao liu yue-ping liu Wei-Dong Huang College of Food Science &Nutntional Engineenng China Agncultural University Beijing 100083 China Department of Biotechnology Beijing Agricultural College Beijing 102206 China

In order to investigate the transportation and distribution of salicylic acid （SA） from root to aboveground tissues in response to high temperature, the roots of grape plant were fed with 14C-SA before high temperature treatment. Radioactivity results showed that progressive increase in SA transportation from root to aboveground as compared with the control varied exactly with the heat treatment time. Radioactivity results of leaves at different stem heights indicated that the increase in SA amount at the top and middle leaves during the early period was most significant in comparison with the bottom leaves. The up-transportation of SA from root to aboveground tissues was dependent on xylem rather than phloem. Autoradiographs of whole grape plants strongly approved the conclusions drawn above. Root-derived SA was believed to be a fundamental source in response to aboveground high temperature.

关键词： aboveground grape high temperature root salicylic acid

Rapid and quantitative detection of hepatitis B virus

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World Journal of Gastroenterology 2015年第42期21卷 11954-11963页

作者： yue-ping liu Chun-Yan Yao Department of Laboratory Medicine Southwest Hospital the Third Military Medical University

Despite availability of a universal vaccine, hepatitis B virus(HBV) infection has a huge impact on public health worldwide. Accurate and timely diagnosis ofHBV infection is needed. Rapid developments have been made in the diagnostic and monitoring methods for HBV infection, including serological and molecular assays. In clinical practice, qualitative hepatitis B surface antigen(HBs Ag) testing has long served as a diagnostic marker for individuals infected with HBV. More recently, HBs Ag level has been used to predict treatment outcome when determined early during treatment or at baseline. However, identification of HBV DNA positive cases that do not have detectable HBs Ag has encouraged the application of molecular tests. Hence, combination of quantitative detection of HBV DNA and HBs Ag can be used to discriminate patients during the course of HBV infection and to monitor therapy. This article reviews the most commonly used quantitative methods for HBs Ag and HBV DNA.

关键词： Hepatitis B virus Biosensor Polymerase chain react

Effects of Temperature Acclimation Pretreatment on the Ultrastructure of Mesophyll Cells in Young Grape Plants （Vitis vinifera L. cv. Jingxiu） Under Cross-Temperature Stresses

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Journal of Integrative Plant Biology 2005年第8期47卷 959-970页

作者： Jun-Huan ZHANG Wei-Dong HUANG yue-ping liu Qiu-Hong PAN College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

Leaves from annual young grape plants （Vitis vinifera L. cv. Jingxiu） were used as experimental materials. The ultrastructural characteristics of mesophyll cells in chilling-treated plants after heat acclimation （HA） and in heat-treated plants after cold acclimation （CA） were observed and compared using transmission electron microscopy. The results showed that slight injury appeared in the ultrastructure of mesophyll cells after either HA （38℃ for 10 h） or CA （8℃ for 2.5 d）, but the tolerance to subsequent extreme temperature stress was remarkably improved by HA or CA pretreatment. The increases in membrane permeability and malondialdehyde concentration under chilling （0℃） or heat （45℃） stress were markedly inhibited by HA or CA pretreatment. The mesophyll cells of plants not pretreated with HA were markedly damaged following chilling stress. The chloroplasts appeared irregular in shape, the arrangement of the stroma lamellae was disordered, and no starch granules were present. The cristae of the mitochondria were disrupted and became empty. The nucleus became irregular in shape and the nuclear membrane was digested. In contrast, the mesophyll cells of HA-pretreated plants maintained an intact ultrastructure under chilling stress. The mesophyll cells of control plants were also severely damaged under heat stress. The chloroplast became round in shape, the stroma lamellae became swollen, and the contents of vacuoles formed clumps. In the case of mitochondria of control plants subjected to heat stress, the outer envelope was digested and the cristae were disrupted and became many small vesicles. Compared with cellular organelles in control plants, those in CA plant cells always maintained an integrated state during whole heat stress, except for the chloroplasts, which became round in shape after 10 h heat stress. From these data, we suggest that the stability of mesophyll cells under chilling stress can be increased by HA pretreatment. Similarly, CA pretreatment can p

关键词： chilling stress cold acclimation cross-adaptation grape (Vitis vinifera L. cv. Jingxiu) plants heat acclimation heat stress ultrastructure.

Research on software development of air temperature prediction in coal face

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Journal of Coal Science & Engineering(China) 2011年第3期17卷 294-297页

作者： QIN yue-ping liu Hong-bo WANG Ke liu Jiang-yue State Key Laboratory of Coal Resources and Safe Mining China University of Mining and Technology （Beijing） Beijing 100083 China

With ever-increasing depth of coal mine and the continuous improvement of mechanization, heat damage has become one of the major disasters in coal mine exploitation. Established the temperature prediction models suitable for different kinds of tunnels through analysis of the heat of shafts, roadways and working faces. The average annual air temperature prediction equation from the inlets of shafts to the working faces was derived. The formula was deduced using combine method of iteration and direct calculation. The method can improve the precision of air temperature prediction, so we could establish the whole pathway air temperature prediction model with high precision. Emphasizing on the effects of leakage air to air temperature of working face and using the ideology of the finite difference method and considering the differential equation of inlet and outlet at different stages, this method can significantly improve the accuracy of temperature prediction. Program development uses Visual Basic 6.0 Language, and the Origin software was used to fit the relevant data. The predicted results shows that the air temperature generally tends to rapidly increase in the air inlet, then changes slowly on working face, and finally increases sharply in air outlet in the condition of goaf air leakage. The condition is in general consistent with the air temperature change tendency of working face with U-type ventilation system. The software can provide reliable scientific basis for reasonable ventilation, cooling measures and management of coal mine thermal hazards.

关键词： finite difference method coal face air temperature prediction prediction methods

Undifferentiated embryonal sarcoma of liver in an old female:Case report and review of the literature

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World Journal of Gastroenterology 2008年第47期14卷 7267-7270页

作者： Li Ma yue-ping liu Cui-Zhi Geng Zhi-Hui Tian Guo-Xiang Wu Xiao-Ling Wang Department of Surgery the ForthHospital of Heibei Medical Univercity No. 12 Jiangkang RoadShijiazhuang 050011 Hebei Province China Departmentof Pathology the Forth Hospital of Heibei Medical UnivercityNo. 12 Jiangkang Road Shijiazhuang 050011 Hebei ProvinceChina Department of Computerized TomographicScan the Forth Hospital of Heibei Medical Univercity No. 12Jiangkang Road Shijiazhuang 050011 Hebei Province China

The clinical characteristics of undifferentiated(embryonal) sarcoma of the liver(UESL) were investigated and the best treatment modalities were *** histology and immuno-histochemistry demonstrated the cellular features of this peculiar *** tumor size was 12 cm × 9 cm × 8 cm in the right liver *** patient underwent surgical resection of the *** postoperative recovery was uneventful and she died eight months after *** tumor showed mixed spindle and polygonal cells within the myxoid *** tumor cells contained eosinophilic hyaline globules that were positive for resistant *** showed positive *** and spindle cells were positively stained with alpha-1-antichymotrypsin(AACT) and *** case indicates that UESL is not obviously differentiated in old-aged adults.

关键词： Undifferentiated (embryonal) sarcoma Old-aged adult Immunohistochemistry Liver tumor

Pre-clinical study of human umbilical cord mesenchymal stem cell transplantation for the treatment of traumatic brain injury: safety evaluation from immunogenic and oncogenic perspectives

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Neural Regeneration Research 2022年第2期17卷 354-361页

作者： Gang Wang Hua-Ling Wu yue-ping liu De-Qi Yan Zi-Lin Yuan Li Chen Qian Yang Yu-Song Gao Bo Diao Basic Medical Laboratory General Hospital of the Central Theater CommandWuhanHubei ProvinceChina Hubei Key Laboratory of Central Nervous System Tumor and Intervention WuhanHubei ProvinceChina Department of Clinical Laboratory The Third People’s Hospital of YongzhouYongzhouHunan ProvinceChina Department of Neurosurgery 990th Hospital of Joint Logistic Support Troops of PLAZhumadianHenan ProvinceChina

Stem cell therapy is a promising strategy for the treatment of traumatic brain injury(TBI). However, animal experiments are needed to evaluate safety;in particular, to examine the immunogenicity and tumorigenicity of human umbilical cord mesenchymal stem cells(hu MSCs) before clinical application. In this study, hu MSCs were harvested from human amniotic membrane and umbilical cord vascular tissue. A rat model of TBI was established using the controlled cortical impact method. Starting from the third day after injury, the rats were injected with 10 μL of 5 × 10^(6)/m L hu MSCs by cerebral stereotaxis or with 500 μL of 1 × 10^(6)/m L hu MSCs via the tail vein for 3 successive days. hu MSC transplantation decreased the serum levels of proinflammatory cytokines in rats with TBI and increased the serum levels of anti-inflammatory cytokines, thereby exhibiting good immunoregulatory function. The transplanted hu MSCs were distributed in the liver, lung and brain injury sites. No abnormal proliferation or tumorigenesis was found in these organs up to 12 months after transplantation. The transplanted hu MSCs negligibly proliferated in vivo, and apoptosis was gradually observed at later stages. These findings suggest that hu MSC transplantation for the treatment of traumatic brain injury displays good safety. In addition, hu MSCs exhibit good immunoregulatory function, which can help prevent and reduce secondary brain injury caused by the rapid release of inflammatory factors after TBI. This study was approved by the Ethics Committee of Wuhan General Hospital of PLA(approval No. 20160054) on November 1, 2016.

关键词： cell transplantation immune regulation inflammation mesenchymal stem cells safety evaluation immunogenicity traumatic brain injury tumorigenesis

Time to stop debating about the everlasting story:Some issues concerning serum total calcium and ionized calcium

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Chinese Medical Journal 2020年第10期133卷 1251-1252页

作者： Ming Li Chao Wang En-Wu Zhou yue-ping liu Department of Clinical Laboratory The 991st Hospital of Joint Logistic Support Troops of People's Liberation ArmyXiangyangHubei 441003China.

To the Editor:We appreciate the interesting and attentive article titled"Predictive accuracy of serum total calcium for both critically high and critically low ionized calcium in critical illness,"written by Hu et al.[1]The use of serum total calcium and its corrected value in predicting critical ionized calcium concentration in critical illness is ***,the authors retrospectively searched the Medical Information for Intensive Care III database to determine whether the level of total calcium,either corrected for albumin or not,could predict critically high and low ionized *** authors concluded that both uncorrected and corrected total calcium levels are unreliable indexes for predicting the critical values of ionized calcium in critical *** is fully believed that this article will provide a profound presentation of and supplement to the everlasting story of calcium state estimation by total *** completely understand the story between total calcium and ionized calcium and comprehensively interpret the results regarding calcium in this manuscript,some issues should first be prioritized.

关键词： corrected critically critical

Diagnostic indexes of a rapid immunoglobulin G/immunoglobulin M combined antibody test for severe acute respiratory syndrome coronavirus 2

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Chinese Medical Journal 2021年第4期134卷 475-477页

作者： Ying liu yue-ping liu Bo Diao Jin-Ya Ding Meng-Xue Yuan Fei-Fei Ren yue Wang Qian-Chuan Huang Department of Laboratory Medicine General Hospital of the Central Theater CommandWuhanHubei 430015China Department of Medical Laboratory Center General Hospital of the Central Theater CommandWuhanHubei 430015China.

Detection of antibodies against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a supplemental option for reverse transcription polymerase chain reaction(RT-PCR)detection,regardless of whether it is immunoglobulin M(IgM),immunoglobulin G(IgG)or combined ***,there are inadequate data on the sensitivity and specificity of IgM/IgG detection,which is of paramount importance for the decision of whether,when,and how to use IgM/IgG antibody *** aim of this study was to evaluate the sensitivity,specificity,accuracy,positive predictive value(PPV),and negative predictive value(NPV)of IgM/IgG antibody detection,especially the anti-interference ability and kappa coefficient compared with the SARS-CoV-2 RT-PCR,which was defined as a standard diagnosis in this study.

关键词： specificity immunoglobulin respiratory