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Ubiquitination in osteosarcoma:unveiling the impact on cell biology and therapeutic strategies

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Cancer Biology & Medicine 2024年第10期21卷 880-897页

作者： Jianlin Shen yue lai Yanjiao Wu Xuan Lin Cheng Zhang Huan Liu Department of Orthopedics Affiliated Hospital of Putian UniversityPutian 351100China Central Laboratory Affiliated Hospital of Putian UniversityPutian 351100China Department of Orthopedics Affiliated Hospital of Guangdong Medical UniversityZhanjiang 524000China Shunde Hospital Southern Medical University(The First People's Hospital of Shunde)Foshan 528000China Department of Environmental and Biological Engineering Putian UniversityPutian 351100China Department of Trauma Center Zhongda HospitalSoutheast UniversityNanjing 210000China Department of Orthopedics The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical UniversityLuzhou 646000China

Ubiquitination,a multifaceted post-translational modification,regulates protein function,degradation,and gene *** pivotal role of ubiquitination in the pathogenesis and progression of cancer,including colorectal,breast,and liver cancer,is ***,an aggressive bone tumor predominantly affecting adolescents,also exhibits dysregulation of the ubiquitination system,encompassing both ubiquitination and deubiquitination *** dysregulation is now recognized as a key driver of osteosarcoma development,progression,and *** review highlights recent progress in elucidating how ubiquitination modulates tumor behavior across signaling *** then focus on the mechanisms by which ubiquitination influences osteosarcoma cell ***,we discuss the potential for targeting the ubiquitin-proteasome system in osteosarcoma *** unraveling the impact of ubiquitination on osteosarcoma cell physiology,we aim to facilitate the development of novel strategies for prognosis,staging,treatment,and overcoming chemoresistance.

关键词： Ubiquitination osteosarcoma cancer development therapeutic target

Gastric ectopic pancreas combined with synchronous multiple early gastric cancer: A rare case report

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World Journal of Clinical Cases 2023年第7期11卷 1569-1575页

作者： Zhen-Ya Zhao yue-Xing lai Ping Xu Department of Gastroenterology Songjiang Hospital Affiliated to Shanghai Jiaotong University School of Medicine(Preparatory Stage)Shanghai 201600China

BACKGROUND A large percentage of patients with ectopic pancreas are *** present,the symptoms are typically *** lesions are predominantly located in the stomach and benign in *** multiple early gastric cancer(SMEGC)(two or more simultaneous malignant lesions with early gastric cancer)is relatively rare and particularly easy to overlook during endoscopic *** prognosis of SMEGC is generally *** report a rare case of ectopic pancreas with concomitant *** SUMMARY A 74-year-old woman presented with paroxysmal upper abdominal *** initial investigations,she tested positive for Helicobacter pylori(***).She underwent esophagogastroduodenoscopy which revealed a 1.5 cm×2 cm major lesion at the greater curvature and a 1 cm minor lesion at the lesser curvature of the *** endoscopic ultrasound,the major lesion showed hypoechoic changes,uneven internal echoes and unclear boundaries between some areas and the muscularis *** submucosal dissection was performed to excise the minor lesion.A laparoscopic resection was chosen for the major *** histopathological examination,the major lesion contained high grade intraepithelial neoplasia with a small focus of cancer.A separate underlying ectopic pancreas was found under this *** minor lesion contained high grade intraepithelial *** this case,the patient was diagnosed with SMEGC with concomitant ectopic pancreas in the *** Patients with atrophy,***,and other risk factors should be carefully investigated to avoid missing other lesions including SMEGC and ectopic pancreas.

关键词： Synchronous multiple early gastric cancer Ectopic pancreas Pathology Case report

Prussian Blue Nanoparticles with High Oxidase-like Activity for Simultaneous Detection of Ascorbic Acid and Alkaline Phosphatase in Real Samples

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Chemical Research in Chinese Universities 2024年第6期40卷 1023-1032页

作者： TANG Zeng lai yue LU Hao XIA Yi JIANG Xue XIONG Xiaoli XIAO Ting College of Chemistry and Material Science Key Laboratory of the Evaluation and Monitoring of Southwest Land ResourcesMinistry of EducationSichuan Normal UniversityChengdu 610068P.R.China

Prussian blue nanoparticles (PB NPs) have exhibited favorable anti-inflammatory effects in animal models,and have been proved to mimic three antioxidant enzymes: peroxidase,catalase,and superoxide *** the oxidase-like activity of PB NPs has not been *** this work,PB NPs with outstanding oxidase-like activity were demonstrated by virtue of the colorimetric substrate o-phenylenediamine (OPD).Interestingly,based on the oxidase-like activity,PB NPs can also catalytically oxidize vitamin B1 (VB1) to strongly fluorescent product in the physiological environment without the addition of other *** that VB1 has no toxicity and carcinogenicity comparing with OPD,a novel PB NPs-VB1 fluorescent sensor was further developed for simple,nontoxic,and rapid detection of ascorbic acid (AA) in foods and alkaline phosphatase (ALP) in human serum samples by taking advantage of the reduction of PB NPs to Prussian White by AA or enzymatic hydrolysate of ALP and ascorbic acid 2-phosphate (AA2P);leading to the decrease of fluorescence *** extremely low detection limits of 44 nmol/L for AA and 72 mU/L for ALP as well as the excellent applicability in real samples were both ***,we envision that such a novel fluorescent probe would be potentially applied in the food analysis and clinic diagnosis fields.

关键词： Prussian blue nanoparticles Oxidase-like activity Fluorescence probe Ascorbic acid Alkaline phosphatase

Dynamic Bonds Mediate π-π Interaction via Phase Locking Effect for Enhanced Heat Resistant Thermoplastic Polyurethane

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Chinese Journal of Polymer Science 2021年第2期39卷 154-163,I0005页

作者： yue lai Xiao Kuang Wen-Hong Yang Yu Wang Ping Zhu Jing-Pu Li Xia Dong Du-Jin Wang CAS Key Laboratory of Engineering Plastics CAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China The George W.Woodruff School of Mechanical Engineering Georgia Institute of TechnologyAtlantaGeorgia 30332United States College of Materials Science and Engineering Shenzhen UniversityShenzhen 518060China

Stimulus-responsive polymers containing dynamic bonds enable fascinating properties of self-healing,recycling and reprocessing due to enhanced relaxation of polymer chain/network with labile ***,we study the structure and properties of a new type of thermoplastic polyurethanes(TPUs)with trapped dynamic covalent bonds in the hard-phase domain and report the frustrated relaxation of TPUs containing weak dynamic bond andπ-πinteraction in hard *** detected by rheometry,the aromatic TPUs with alkyl disulfide in the hard segments possess the maximum network relaxation time in contrast to those without dynamic bonds and alicyclic *** situ FTIR and small-angle scattering results reveal that the alkyl disulfide facilitates stronger intermolecular interaction and more stable micro-phase morphology inπ-πinteraction based aromatic *** dynamics simulation for pure hard segments of model molecules verify that the presence of disulfide bonds leads to strongerπ-πstacking of aromatic rings due to both enhanced assembling thermodynamics and *** enhancedπ-πpacking and micro-phase structure in TPUs further kinetically immobilize the dynamic *** kinetically interlocking between the weak dynamic bonds and strong molecular interaction in hard segments leads to much slower network relaxation of *** work provides a new insight in tuning the network relaxation and heat resistance as well as molecular self-assembly in stimulus-responsive dynamic polymers by both molecular design and micro-phase control toward the functional applications of advanced materials.

关键词： Thermoplastic polyurethane Disulfide bond Stimuli-responsive rearrangement π-πStacking Phase locked effect

15-PGDH is reduced and induces apoptosis and cell cycle arrest in gastric carcinoma

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World Journal of Gastroenterology 2012年第10期18卷 1028-1037页

作者： Li-Hong Lou Da-Dao Jing yue-Xing lai Ying-Ying Lu Ji-Kun Li Kai Wu International Medical Care Center Shanghai First People＇s Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200080 China Department of Gastroenterology Shanghai First People＇s Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200080 China Department of Surgery Shanghai First People＇s Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200080 China

AIM: To investigate the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) in human gastric cancer and it's mechanism in apoptosis and cell cycle arrest. METHODS: Expression of 15-PGDH mRNA and protein was examined by immunohistochemistry, immunocytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting in tissue from human gastric cancer, gastric precancerous state (gastric polyps and atrophic gastritis), normal stomach, and gastric cancer cell lines. The relationship between gastric cancer, gastric precancerous state and 15-PGDH expression was determined. The association between expression of 15-PGDH and various clinicopathological parameters in gastric cancer was evaluated. Human gastric cancer cell line SGC-7901 was transfected with 15-PGDH expression plasmids. The effect of 15-PGDH on the cell cycle was examined by flow cytometry. The effect of 15-PGDH on apoptosis was examined by transmission electron microscopy, flow cytometry and transferasemediated nick end labeling (TUNEL) assay. Expression of cell cycle (p21, p27, p16 and p53) and apoptosis (Survivin, BCL-2, BCL-XL, BAK and BAX) genes was analyzed by RT-PCR. RESULTS: Expression of 15-PGDH mRNA and protein in human gastric cancer tissues was significantly lower than in normal gastric tissues (P < 0.01). Expression in human gastric cancer cell lines MKN-28 and MKN-45 was reduced, and absent in SGC-7901 cells (P < 0.05). Reduction of 15-PGDH expression was also found in precancerous tissues, such as gastric polyps and atrophic gastritis (P < 0.01). There was a significant difference in expression of 15-PGDH among various gastric cancer pathological types (P < 0.05), with or without distant metastasis (P < 0.05) and different TNM stage (P < 0.01). Flow cytometry demonstrated a significant increase in apoptotic cells in SGC-7901 cells transfected with pcDNA3/15-PGDH plasmid for 24 and 48 h (P < 0.01), and an increased fraction of sub-G1 phase after transfection (P <

关键词： Gastric carcinoma 15-hydroxyprostaglandin dehydrogenase Apoptosis Cell cycle arrest Tumor growth

Cytogenetic characteristics of B cell chronic lymphocytic leukemia in 275 Chinese patients by fluorescence in situ hybridization： a multicenter study

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Chinese Medical Journal 2011年第16期124卷 2417-2422页

作者： lai yue-yun HUANG Xiao-jun Peking University Institute of Hematology Peking UniversityPeople's Hospital Beijing Key Laboratory of Hematopoietic StemCell Transplantation Beijing 100044 China

Background Under conventional cytogenetic （CC） analysis, only 30%-50% of B cell chronic lymphocytic leukemia （B-CLL） cases show clonal aberrations. Using fluorescence in situ hybridization （FISH）, the percentage of patients with abnormalities rises to almost 80%, among them, the most frequent abnormalities were 13q14, 1 lq22, p53 deletions and trisomy 12. The aim of this study was to explore the incidence of cytogenetic changes in Chinese patients with B-CLL. Methods We used FISH methods to detect the cytogenetic features in 275 cases of B-CLL from 48 hospitals. The correlation between FISH abnormalities and clinical characteristics such as age, gender, white blood cell count, peripheral hemoglobin （Hb） level, peripheral platelet count （PLT）, lactate dehydrogenase （LDH） level, Rai stage, Binet stage, and overall survival was analyzed, and the relationship between them and overall survival was also analyzed to evaluate their prognostic implications. Results Of the 275 patients, genetic aberrations were found in 77.8% using FISH. The frequencies of abnormalities were as follows： 13q deletion （56.4%）, trisomy 12 （34.5%）, p53 deletion （33.5%） and 11q22 deletion （30.5%）. It was obvious that the patients with p53 deletion had lower level of Hb （P=0.001） and PLT （P=-0.003） when compared to patients without p53 deletion. Significant differences were obtained in the distribution of p53 deletion according to Rai and Binet classification systems （P=0.016 and 0.008 respectively）. Significant differences were also observed when the overall survival was correlated with p53 deletion （P=-0.043）, Rai stage （P=0.006）, Binet stage （P=0.013）, Hb level （P=-0.004） and PLT level （P=-0.010）. Conclusions Chinese CLL patients have the similar frequencies of del（13q）, trisomy 12, del（11q） and a higher frequency of del（17p） when compared to literatures. Del（17p） is associated with advanced stage and low levels of Hb and PLT. Patients with p53 deletion, or advanced stage probably have poor surv

关键词： cytogenetics chronic lymphocytic leukemia in situ hybridization, fluorescence

Effect of Acupuncture Different Points on Unstable Bladder

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Journal of Acupuncture and Tuina Science 2003年第3期1卷 22-24页

作者： CHEN yue-lai Shanghai Acupuncture-moxibustion-Tuina Medical Center Yueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of TCM China Shanghai Research Institute of Acupuncture and Meridian China

Purpose To observe the effect of acupuncturing different points on unstable *** Fifty-one patients of unstable bladder were divided randomly into Shu-point group, Mu-point group, He-point group, observe the change of symptom score and *** Puncturing these points can improve the symptom score and urodynamics, but the different points can get different effect, and the effect of Shu-point and He-point is superior to that of *** The different points of bladder have different adjusting function and that function relates with nerves locating in points. Key words Urodynamics - Acupuncture Therapy - Bladder Disease - Syndrome Founhdation Item: Clinical Study and Neural Receptor Mechanism of Lumbosacral acupoints Regulating Unstable Bladder (02CK02), supported by the Education Committee of Shanghai City.Translator: CHEN yue-lai

关键词： Urodynamics Acupuncture Therapy Bladder Disease Syndrome

Correction of distorted X-ray absorption spectra collected with capillary sample cell

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Nuclear Science and Techniques 2023年第7期34卷 179-188页

作者： Hao Wang yue-Cheng lai Jia-Jun Zhong Yun-Peng Liu Lei Yao Li-Xiong Qian Xue-Qing Xing Zhong-Jun Chen Guang Mo Zhong-Hua Wu Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China School of Materials Science and Engineering Northeastern UniversityShenyang 110819China

In certain exceptional cases,capillary samples must be used to measure X-ray absorption spectra(XAS).However,the inho-mogeneous thickness of capillary samples causes XAS *** study discusses the distortion and correction of the XAS curve caused by the inhomogeneous thickness of capillary *** relationship between the distorted XAS curveμ′d_(eq)(measured values)and the real absorption coefficientμ_(s)d_(eq)(true values)of the sample was *** distortion was slight and negligible when the vertical size(2h)of the X-ray beam spot was smaller than 60%of the capillary tube’s inner diameter(2R_(in)).When h/R_(in)>1,X-ray leakage is inevitable and should be avoided during *** X-ray leakage caused by an X-ray beam spot size larger than the inner diameter of the capillary tube leads to serious compressed distortion of the XAS *** h/R_(in)<1,the distorted XAS data were well *** errors and their influence on the corrected XAS are also *** and corrections for distortions verify the feasibility and effectiveness of the corrected method.

关键词： XAS Capillary Compression distortion Correction Python

The effect of sensation of transmission along meridian acupuncture for chronic nonbacterial prostatitis

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World Journal of Acupuncture-Moxibustion 2019年第2期29卷 113-118页

作者： Shu-ren MING Yu-lin FU Wen-guang HOU yue-lai CHEN yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine Shanghai 200437 China

Objective:To investigate the short-term efficacy difference between sensation of transmission along meridian acupuncture and non-sensation of transmission along meridian acupuncture in the treatment of chronic nonbacterial ***:A randomized,controlled,and single-blind clinical study was performed to collect 63 patients with chronic nonbacterial prostatitis who met the inclusion criteria and were randomly assigned to a transmission sensation group(31 patients)and a non-transmission sensation group(32 patients).The two groups of patients took the same acupuncture *** they were lying down on their backs,Guānyuán(关元 CV4),Zhōngjí(中极 CV3)and Dàhè(大赫 K112)were *** they were lying down on their stomachs,Shènshū(肾俞 BL 23),Cìliáo(次髎 BL32)and Huìyáng(会阳 BL35)were *** them,the transmission sensation group was treated with transmission sensation along meridian to apply deep needling to the lower abdomen and lumbosacral acupuncture points with lifting,thrusting,and twirling method,so that the needling sensation was transmitted to the bladder,perineum and *** non-transmission sensation group was treated with non-transmission sensation along meridian,and the acupuncture points were treated with conventional acupuncture until the patient felt soreness and distending *** the arrival of *** groups of patients were treated with electro-acupuncture during acupuncture,and the sparse-dense wave(sparse wave 4 Hz,dense wave 20 Hz),and the needle retention continued for 20 *** patients were treated for 3 times with once every other day,and the treatment lasted for one *** CV4,CV3 and KI12 were used in the first and third treatments,and the BL23,BL32 and BL35 were used in the second *** National Institute of Health chronic prostatitis syndrome index(NIH-CPSI)and Hamilton Anxiety Rating Scale(HAMA)were observed,and the clinical efficacies of the patient were ***:After treatment,the NIH-CPSI scores(18.29±1.25,

关键词： Acupuncture Transmission sensation along meridian Chronic nonbacterial prostatitis