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Journal of Genetics and Genomics 2024年第1期51卷 61-74页

作者： Cheng Ji yue ou Wangjianfei Yu Jiaxin Lv Fanmiao Zhang Huashan Li Zeyun Gu Jiayuan Li Zhaomin Zhong Han Wang Center for Circadian Clocks Soochow UniversitySuzhouJiangsu 215123China School of Biology and Basic Medical Sciences Suzhou Medical CollegeSoochow UniversitySuzhouJiangsu 215123China

Thyroid-stimulating hormone(TSH)is important for the thyroid gland,development,growth,and *** in TSH production or the thyrotrope cells within the pituitary gland cause congenital hypothyroidism(CH),resulting in growth retardation and neurocognitive *** human TSH is known to display rhythmicity,the molecular mechanisms underlying the circadian regulation of TSH and the effects of TSH-thyroid hormone(TH)signaling on the circadian clock remain *** we show that TSH,thyroxine(T4),triiodothyronine(T3),and tshba display rhythmicity in both larval and adult zebrafish and tshba is regulated directly by the circadian clock via both E′-box and *** tshba^(−/−)mutants manifest congenital hypothyroidism,with the characteristics of low levels of T_(4)and T_(3)and growth *** or overexpression of tshba alters the rhythmicity of locomotor activities and expression of core circadian clock genes and hypothalamic-pituitary-thyroid(HPT)axis-related ***,TSH-TH signaling regulates clock2/npas2 via the thyroid response element(TRE)in its promoter,and transcriptome analysis reveals extensive functions of Tshba in ***,our results demonstrate that zebrafish tshba is a direct target of the circadian clock and in turn plays critical roles in circadian regulation along with other functions.

关键词： Thyroid-stimulating hormone subunitβa (tshba) Thyroid hormone Circadian rhythm TALEN Zebrafish

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司法解释进入行政执法的理论逻辑

行政法学研究 2024年第1期 168-176页

作者：陈越瓯吉林大学法学院吉林长春130012

相较于在司法系统内部的权威性地位,司法解释对于行政执法的效力尚不明确。针对我国现行法律解释体制以法律解释权力的配置为中心的现状,在司法解释能否进入行政执法的问题上,理论上存在基于法律解释工作安排的权力分工障碍、基于专业... 详细信息

相较于在司法系统内部的权威性地位,司法解释对于行政执法的效力尚不明确。针对我国现行法律解释体制以法律解释权力的配置为中心的现状,在司法解释能否进入行政执法的问题上,理论上存在基于法律解释工作安排的权力分工障碍、基于专业知识储备的权力能力障碍和基于权力配置原则的权力性质障碍。通过对实践中行政机关自发援引司法解释现象的经验分析,只有权力性质障碍构成司法解释进入行政执法的真正阻碍。确定司法解释外部效力之范围和强度的前提,是在功能主义的国家权力配置原则下重构司法解释权和行政解释权之间的关系。依据法律解释体制提供统一法律指引的功能目标和社会主义法律传统对国家机构间关系的定位,在现行法律解释体制下,司法解释权和行政解释权之间应当形成一种对等结构,在这种权力结构中,司法解释具有一定程度的外部效力。司法解释虽然不构成行政执法的直接依据,但对行政机关具有可反驳的规范性拘束力。

关键词：司法解释行政执法法律解释权功能主义国家机构

面向生成式人工智能的档案数智化服务应用场景探索

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档案与建设 2024年第9期 83-91页

作者：刘婧欧月华中师范大学档案馆湖北武汉430079 华中师范大学信息管理学院湖北武汉430079

档案数智化服务是数智时代兴起的一种新型档案信息服务形态,兼具数字化和智能化的双重特性,是档案事业创新发展的趋势。生成式人工智能为档案数智化服务探索带来了新机遇。文章首先调查并分析了档案数智化服务的现状;其次,从法律政策、... 详细信息

档案数智化服务是数智时代兴起的一种新型档案信息服务形态,兼具数字化和智能化的双重特性,是档案事业创新发展的趋势。生成式人工智能为档案数智化服务探索带来了新机遇。文章首先调查并分析了档案数智化服务的现状;其次,从法律政策、标准指南、技术需求、风险防控四个角度分析了生成式人工智能应用于档案数智化服务的可行性;最后,构建档案数据的高效整理与检索、档案利用的流程优化与重塑、档案内容的深度挖掘与分析、档案服务的空间延伸与开放四个服务应用场景。

关键词：档案服务数智化服务生成式人工智能应用场景

面向管道焊缝渗透检测机器人的管道定位与规划方法

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科学技术与工程 2024年第17期24卷 7168-7176页

作者：李彰欧越陈建文徐阳樊继壮赵杰中核武汉核电运行技术股份有限公司武汉430200 核动力运行研究所武汉430074 中国核工业集团核工业特种机器人工程技术研究中心武汉430200 哈尔滨工业大学机电学院哈尔滨150000

为了实现核电设施管路的自动化焊缝检测作业,在降低人工作业安全风险的同时提高工作效率,提出了一种集喷涂、喷擦、刷涂、擦洗、环境感知和场景建模的机器人自主作业软件系统及相关算法。分析作业机器人的工作环境和功能需求,进行机械... 详细信息

为了实现核电设施管路的自动化焊缝检测作业,在降低人工作业安全风险的同时提高工作效率,提出了一种集喷涂、喷擦、刷涂、擦洗、环境感知和场景建模的机器人自主作业软件系统及相关算法。分析作业机器人的工作环境和功能需求,进行机械臂和感知模块的选型,制定三维建模和参数辨识方案,搭建焊缝检测作业系统;提出了一种基于机器人感知到的视觉和深度信息进行场景建模的算法,该算法可以输出检测关到作业面的位置、三维模型以及作业区域范围;提出了一种基于三维重构模型和作业区域范围进行作业轨迹规划的算法,并搭建了仿真平台验证运动轨迹的正确性;开展了管道定位与三维重构实验以及轨迹规划实验,实验的结果证明,提出的管道定位方法能够实现精确的点云数据采集和高精度的管道参数辨识,机械臂能够实现准确性高、连续性好的运动,满足管道焊缝检测作业的要求。

关键词：焊缝检测三维点云表面建模运动规划

High-efficiency inspecting method for mobile robots based on task planning for heat transfer tubes in a steam generator

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机械工程前沿:英文版 2023年第2期18卷 161-175页

作者： Biying XU Xuehe ZHANG yue ou Kuan ZHANG Zhenming XING Hegao CAI Jie ZHAO Jizhuang FAN State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 150001China

Many heat transfer tubes are distributed on the tube plates of a steam generator that requires periodic inspection by *** inspection robots are usually involved in issues:Robots with manipulators need complicated installation due to their fixed base;tube mobile robots suffer from low running efficiency because of their structural *** there are thousands of tubes to be checked,task planning is essential to guarantee the precise,orderly,and efficient inspection *** in-service robots check the task tubes using row-by-row and column-bycolumn *** leads to unnecessary inspections,resulting in a long shutdown and affecting the regular operation of a nuclear power ***,this paper introduces the structure and control system of a dexterous robot and proposes a task planning *** method proceeds into three steps:task allocation,base position search,and sequence *** allocate the task regions,this method calculates the tool work matrix and proposes a criterion to evaluate a *** then all tasks contained in the sub-region are considered globally to search the base ***,we apply an improved ant colony algorithm for base sequence planning and determine the inspection orders according to the planned *** validated the optimized algorithm by conducting task planning experiments using our robot on a tube *** results show that the proposed method can accomplish full task coverage with few repetitive or redundant inspections and it increases the efficiency by 33.31% compared to the traditional planning algorithms.

关键词： steam generator transfer tubes mobile robot dexterous structure task planning efficient inspection

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兰科植物与共生真菌互作关系的研究进展

分子植物育种 2024年第8期22卷 2778-2784页

作者：谢泰祥郑清冬王艺兰琳英徐艳芳欧悦姚亚合艾叶福建农林大学园林学院兰科植物保护与利用国家林业与草原局重点实验室福州350002

兰科植物的种子细小,是所有被子植物中最小种子类型之一,只具备一种不被胚乳包围且小而不发达的胚,在其生命周期的整个过程中,特别是在营养资源匮乏的早期阶段,对共生真菌的依赖性非常强。共生真菌与兰科植物形成菌根联合共生体为种子... 详细信息

兰科植物的种子细小,是所有被子植物中最小种子类型之一,只具备一种不被胚乳包围且小而不发达的胚,在其生命周期的整个过程中,特别是在营养资源匮乏的早期阶段,对共生真菌的依赖性非常强。共生真菌与兰科植物形成菌根联合共生体为种子萌发、原球茎生长发育、幼苗分化以及成年植株生殖生长提供所需的营养物质。本研究对兰科植物共生真菌的研究历程与概况、菌根互作关系、多样性与专一性、促生作用以及应用前景等方面进行了综述,并在此基础上进行展望,以期为兰科植物野生资源保育与开发、人工栽培生产以及生防菌与菌肥研发等研究提供参考。

关键词：兰科植物共生真菌多样性与专一性促生作用

Knockdown of HE4 suppresses tumor growth and invasiveness in lung adenocarcinoma through regulation of EGFR signaling

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Oncology Research 2024年第6期32卷 1119-1128页

作者： yue ZHANG WENYU YANG XIAOWANG HAN yue QIAO HAITAO WANG TING CHEN TIANYING LI WEN-BIN ou Department of Biopharmaceutics College of Life Sciences and MedicineZhejiang Provincial Key Laboratory of Silkworm Bioreactor and BiomedicineZhejiang Sci-Tech UniversityHangzhou310018China Department of Thoracic Surgery Zhejiang Provincial People’s HospitalHangzhou310014China

It has been shown that the high expression of human epididymis protein 4(HE4)in most lung cancers is related to the poor prognosis of patients,but the mechanism of pathological transformation of HE4 in lung cancer is still *** current study is expected to clarify the function and mechanism of HE4 in the occurrence and metastasis of lung adenocarcinoma(LUAD).Immunoblotting evaluated HE4 expression in lung cancer cell lines and biopsies,and through analysis of The Cancer Genome Atlas(TCGA)*** HE4 overexpression was demonstrated in LUAD,but not in lung squamous cell carcinoma(LUSC),indicating that HE4 can serve as a biomarker to distinguish between LUAD and ***4 knockdown significantly inhibited cell growth,colony formation,wound healing,and invasion,and blocked the G1-phase of the cell cycle in LUAD cell lines through inactivation of the EGFR signaling downstream including PI3K/AKT/mTOR and RAF/MAPK *** first-line EGFR inhibitor gefitinib and HE4 shRNA had no synergistic inhibitory effect on the growth of lung adenocarcinoma cells,while the third-line EGFR inhibitor osimertinib showed additive anti-proliferative ***,we provided evidence that HE4 regulated EGFR expression by transcription regulation and protein interaction in *** findings suggest that HE4 positively modulates the EGFR signaling pathway to promote growth and invasiveness in LUAD and highlight that targeting HE4 could be a novel strategy for LUAD treatment.

关键词： Lung adenocarcinoma Human epididymis protein 4 Epidermal growth factor receptor Biomarker Targeted therapies

Effect of culturing conditions on the expression of key enzymes in the proteolytic system of Lactobacillus bulgaricus

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第4期16卷 317-326页

作者： Jun-cai Hou Fei LIU Da-xi REN Wei-wei HAN yue-ou DU Key Laboratory of Dairy Science Ministry of Education Northeast Agricultural University College of Food Science Northeast Agricultural University College of Animal Sciences Zhejiang University

The proteolytic system of Lactobacillus bulgaricus breaks down milk proteins into peptides and amino acids, which are essential for the growth of the bacteria. The aim of this study was to determine the expressions of seven key genes in the proteolytic system under different culturing conditions （different phases, initial pH values, temperatures, and nitrogen sources） using real-time polymerase chain reaction （RT-PCR）. The transcriptions of the seven genes were reduced by 30-fold on average in the stationary phase compared with the exponential growth phase The transcriptions of the seven genes were reduced by 62.5-, 15.0-, and 59.0-fold in the strains KLDS 08006, KLDS 08007, and KLDS 08012, respectively, indicating that the expressions of the seven genes were significantly different among strains. In addition, the expressions of the seven genes were repressed in the MRS medium containing casein peptone. The effect of peptone supply on PepX transcription was the weakest compared with the other six genes, and the impact on OppD transcription was the strongest. Moreover, the expressions of the seven genes were significantly different among different strains （P〈0.05）. All these results indicated that the culturing conditions affected the expression of the proteolytic system genes in Lactobacillus bulgaricus at the transcription level.

关键词： Gene expression Proteolytic system Lactobacillus bulgaricus Proteinase

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玉米黄粉可降解性塑料的制备和性质

农业工程学报 2009年第5期25卷 284-287页

作者：陈野王冠禹杜悦食品营养与安全省部共建教育部重点实验室天津科技大学食品工程与生物技术学院天津300457

玉米黄粉是湿法玉米淀粉加工的副产物。为充分利用这种副产物生产可降解塑料,将其与增塑剂甘油混合后,经单螺杆挤压机制成母粒,母粒再被热压成型为试验片,并对试验片的性质进行了分析,结果表明:含有20%甘油的试验片具有良好的机械性质;... 详细信息

玉米黄粉是湿法玉米淀粉加工的副产物。为充分利用这种副产物生产可降解塑料,将其与增塑剂甘油混合后,经单螺杆挤压机制成母粒,母粒再被热压成型为试验片,并对试验片的性质进行了分析,结果表明:含有20%甘油的试验片具有良好的机械性质;以Halsey方程为基础,建立的模型能预测试验片拉伸强度与相对湿度关系,经验证模型与实际测量值具有良好的拟合性;应用扫描电子显微镜观察试验片内部结构的结果表明它具有均匀的结构;热重分析的结果表明可降解试验片在170℃以下时能保持热稳定性。

关键词：挤压拉伸强度平衡含水率空气湿度机械特性玉米黄粉

High-density lipoprotein regulates angiogenesis by affecting autophagy via miRNA-181a-5p

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Science China(Life Sciences) 2024年第2期67卷 286-300页

作者： Bi-Ang Kang Hua-Ming Li Ya-Ting Chen Meng-Jie Deng Yan Li yue-Ming Peng Jian-Jun Gao Zhi-Wei Mo Jia-Guo Zhou Zhi-Jun ou Jing-Song ou Division of Cardiac Surgery Cardiovascular Diseases InstituteThe First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China National-Guangdong Joint Engineering Laboratory for Diagnosis and Treatment of Vascular Diseases Guangzhou 510080China NHC key Laboratory of Assisted Circulation(Sun Yat-sen University) Guangzhou 510080China Guangdong Provincial Engineering and Technology Center for Diagnosis and Treatment of Vascular Diseases Guangzhou 510080China Division of Vascular Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Department of Pharmacology Cardiac and Cerebral Vascular Research CenterZhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Division of Hypertension and Vascular Diseases Department of CardiologyCardiovascular Diseases InstituteThe First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Guangdong Provincial Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China

We previously demonstrated that normal high-density lipoprotein(nHDL)can promote angiogenesis,whereas HDL from patients with coronary artery disease(d HDL)is dysfunctional and impairs *** plays a critical role in angiogenesis,and HDL regulates ***,it is unclear whether n HDL and d HDL regulate angiogenesis by affecting *** cells(ECs)were treated with n HDL and d HDL with or without an autophagy ***,endothelial nitric oxide synthase(e NOS)expression,miRNA expression,nitric oxide(NO)production,superoxide anion(O2^(·-))generation,EC migration,and tube formation were evaluated.n HDL suppressed the expression of miR-181a-5p,which promotes autophagy and the expression of e NOS,resulting in NO production and the inhibition of O2^(·-)generation,and ultimately increasing in EC migration and tube formation.d HDL showed opposite effects compared to n HDL and ultimately inhibited EC migration and tube *** found that autophagy-related protein 5(ATG5)was a direct target of ***5 silencing or miR-181a-5p mimic inhibited n HDL-induced autophagy,e NOS expression,NO production,EC migration,tube formation,and enhanced O2^(·-)generation,whereas overexpression of ATG5 or miR-181a-5p inhibitor reversed the above effects of d ***5 expression and angiogenesis were decreased in the ischemic lower limbs of hypercholesterolemic low-density lipoprotein receptor null(LDLr^(-/-))mice when compared to C57BL/6 ***5 overexpression improved angiogenesis in ischemic hypercholesterolemic LDLr^(-/-)*** together,nHDL was able to stimulate autophagy by suppressing miR-181a-5p,subsequently increasing e NOS expression,which generated NO and promoted *** contrast,d HDL inhibited angiogenesis,at least partially,by increasing miR-181a-5p expression,which decreased autophagy and e NOS expression,resulting in a decrease in NO production and an increase in O2^(·-)*** findings reveal a nov

关键词： high-density lipoprotein angiogenesis autophagy ATG5 miRNA endothelial nitric oxide synthase