检索结果-南通市图书馆

Microarray Expression Profiling of Postharvest Ponkan Mandarin (Citrus reticulata) Fruit under Cold Storage Reveals Regulatory Gene Candidates and Implications on Soluble Sugars Metabolism

引用

Journal of Integrative Plant Biology 2011年第5期53卷 358-374页

作者： Andan Zhu Wenyun Li Junli Ye Xiaohua Sun yuduan ding Yunjiang Cheng Xiuxin Deng Key Laboratory of Horticulture Plant Biology of Ministry of Education and National Key Laboratory of Crop Genetic Improvement HuazhongAgricultural University Wuhan 430070 China

Low temperature storage is widely applied to maintain citrus postharvest fruit quality. In this study, the transcriptional and metabolic changes in the pulp tissue of Citrus reticulata Blanco cv. ＂Ponkan＂ were studied for three successive months under cold storage by Affymetrix Citrus GeneChip and gas chromatography, respectively. As many as 2 161 differentially expressed transcripts were identified based on the bayesian hierarchical model. The statistical analysis of gene ontology revealed that defense/stress-related genes were induced quickly, while autophagy-related genes were overrepresented in the late sampling stages, suggesting that the functional shift may coincide with the subsequent steps of chilling development. We further classified the potential regulatory components and concluded that ethylene may play the crucial role in chilling development in this non-climacteric fruit. To cope with complex events, 53 upregulated transcription factors represented regulatory candidates. Within these, the AP2-EREBP, C2H2 and AS2 gene family were overrepresented. Cold storage also causes alterations in various metabolic pathways; a keen interest is paid in deciphering expression changes of soluble sugar genes as increased evidence that soluble sugars act as both osmolytes and metabolite signal molecules. Our results will likely facilitate further studies in this field with promising genetic candidates during chilling.

关键词： MANDARIN orange SUGARS PLANT metabolism FRUIT -- Quality PLANT genetics DNA microarrays PLANT gene expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

3RISPR-P 2.0： An Improved CRISPR-Cas9 Too For Genome Editing in Plants

引用

Molecular Plant 2017年第3期10卷 530-532页

作者： Hao Liu yuduan ding Yanqing Zhou Wenqi Jin Kabin Xie Ling-Ling Chen National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P. R. China Agricultural Bioinformatics Key Laboratory of Hubei Province College ofInformatics Huazhong Agricultural University Wuhan 430070 P. R China College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 P. R. China

The clustered regularly interspaced short palindromic repeat （CRISPR） - CRISPR-associated protein 9 （CRISPR-Cas9） system has emerged as a versatile molecular tool for genome editing in various organisms in recent years （Tsai and Joung, 2016）. In this system, the endonuclease of Cas9 is directed to DNA targets by a synthetic guide RNA （sgRNA）.

关键词： An Improved CRISPR-Cas9 Genome Editing various applications

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rice Information GateWay： A Comprehensive Bioinformatics Platform for Indica Rice Genomes

引用

Molecular Plant 2018年第3期11卷 505-507页

作者： Jia-Ming,Song Yang,Lei Cheng-Cheng,Shu yuduan,ding Feng,Xing Hao,Liu Jia,Wang Weibo,Xie Jianwei,Zhang Ling-Ling,Chen National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P. R. China Agricultural Bioinformatics Key Laboratory of Hubei Province College of Informatics Huazhong Agricultural University Wuhan 430070 China [∗]Corresponding author [∗∗]Corresponding author

Dear Editor,Oryza sativa subsp, indica and japonica are two subspecies of Asian cultivated rice, among which indica rice is much more widely grown and genetically diverse. Over the past years, the Rice Annotation Project Database （RAP-DB）（Ohyanagi et al., 2006） and Michigan State University Rice Genome Annotation Project （MSU-RGAP）（Ouyang et al., 2007） are two popular databases that have been developed to manage rice genomic and transcriptomic data based on the unified reference genome of japonica cultivar Nipponbare （International Rice Genome Sequencing Project, 2005）. Beijing Genomics Institute Rice Information System （BGI-RIS）（Zhao et ai., 2004） is an available resource for indica rice cultivar 93-11;

关键词：

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrated transcriptomic and metabolomic analyses of a wax deficient citrus mutant exhibiting jasmonic acid-mediated defense against fungal pathogens

引用

Horticulture Research 2018年第1期5卷 404-417页

作者： Yizhong He Jingwen Han Runsheng Liu yuduan ding Jinqiu Wang Li Sun Xiaoming Yang Yunliu Zeng Weiwei Wen Juan Xu Hongming Zhang Xiang Yan Zhaoxing Chen Zuliang Gu Hong Chen Huanqing Tang Xiuxin Deng Yunjiang Cheng Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhan430070China Institute of Citrus Science Research of Ganzhou Ganzhou341000Jiangxi ProvinceChina Research Center of Navel Orange Planting Technology of Anyuan County Ganzhou341000Jiangxi ProvinceChina

Naturally,resistant crop germplasms are important resources for managing the issues of agricultural product safety and environment *** found a spontaneous mutant of‘Newhall’navel orange(Citrus sinensis Osbeck)(MT)with broad-spectrum protections against fungal pathogens in the orchard,postharvest-storage,and artificial inoculation *** understand the defense mechanism of MT fruit,we constructed a genome-scale metabolic network that integrated metabolome and transcriptome *** coordinated transcriptomic and metabolic data were enriched in two sub-networks,showing the decrease in very long chain fatty acid(by 41.53%)and cuticular wax synthesis(by 81.34%),and increase in the synthesis of jasmonic acid(JA)(by 95.23%)and JA-induced metabolites such as 5-dimethylnobietin(by 28.37%)in ***,cytological and biochemical analyses confirmed that the response to fungal infection in MT was independent of wax deficiency and was correlated with the levels of jasmonates,and the expression of plant defensin gene *** of exogenous application of MeJA and JA inhibitors such as propyl gallate proved that JA-mediated defense contributes to the strong tolerance against pathogens in *** results indicated that jasmonate biosynthesis and signaling are stimulated by the fatty acid redirection of MT,and participate in the tolerance of pathogenic fungi.

关键词： synthesis orange deficient

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

脱落酸处理对苹果成熟、乙烯合成及其信号转导基因表达的影响

引用

食品科学 2021年第17期42卷 225-232页

作者：戚英伟刘悬烁丁毓端姜永华张玉洁刘佳蒋紫洮任小林西北农林科技大学园艺学院陕西杨凌712100 广东省农业科学院蚕业与农产品加工研究所农业农村部功能食品重点实验室广东省农产品加工重点实验室广东广州510610 广东省农业科学院果树研究所农业农村部南亚热带果树生物学与遗传资源利用重点实验室广东省热带亚热带果树研究重点实验室广东广州510640 临潼区林业工作站陕西西安710600

为研究脱落酸(abscisic acid,ABA)对苹果果实采后成熟、乙烯合成及其信号转导基因表达的影响,本实验以‘Granny Smith’苹果果实为实验材料,将其进行外源ABA处理后置于20℃贮藏(以无菌水处理为对照),测定贮藏过程中的硬度、可滴定酸质... 详细信息

为研究脱落酸(abscisic acid,ABA)对苹果果实采后成熟、乙烯合成及其信号转导基因表达的影响,本实验以‘Granny Smith’苹果果实为实验材料,将其进行外源ABA处理后置于20℃贮藏(以无菌水处理为对照),测定贮藏过程中的硬度、可滴定酸质量分数、纤维素酶活力、果胶甲酯酶活力、叶绿素含量、内源ABA含量、乙烯产生速率及乙烯生物合成基因(ACO1、ACO_(2)、ACS1、ACS3)、苹果乙烯信号转导基因(ERS1、ERS2、ETR2、ERF3、ERF4、ERF5)的表达量。结果表明,与对照组相比,ABA处理能降低苹果果实贮藏过程中的硬度和可滴定酸质量分数,提高果实中的纤维素酶和果胶甲酯酶活性,促进叶绿素a、叶绿素b和总叶绿素含量降低。定量聚合酶链式反应分析结果表明,与对照组相比,ABA处理能够显著提高苹果乙烯生物合成基因的表达量(P<0.05)。同时,参与苹果乙烯信号转导的ERS2、ETR2和ERF3、ERF4、ERF5表达量随着果实采后成熟明显增加,并且ABA处理组果实中的表达量总体显著高于对照组(P<0.05)。综上,外源ABA处理能够促进苹果内源ABA的合成,提高苹果乙烯生物合基因和信号转导基因的表达,促进苹果内源乙烯合成和乙烯信号的转导,加快苹果果实的采后成熟进程。

关键词：苹果乙烯脱落酸基因表达果实成熟

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

‘翠香’猕猴桃黑头病常温贮藏期间生理特性研究

引用

西北农业学报 2022年第4期31卷 479-487页

作者：陈丽娟杨亚明戚英伟杜薇殷伟杰任小林丁毓端西北农林科技大学园艺学院陕西杨凌712100 广东省农业科学院蚕桑食品研究所/农业部功能食品重点实验室/广东省农产品加工重点实验室广州510610

通过了解黑头病对‘翠香’猕猴桃常温贮藏期间的影响,为‘翠香’黑头病果实的贮藏管理提供依据。以感病和健康的‘翠香’猕猴桃果实为材料,测定猕猴桃常温贮藏期间的生理特性变化。结果显示,黑头病造成采后‘翠香’果实软化迅速,可溶性... 详细信息

通过了解黑头病对‘翠香’猕猴桃常温贮藏期间的影响,为‘翠香’黑头病果实的贮藏管理提供依据。以感病和健康的‘翠香’猕猴桃果实为材料,测定猕猴桃常温贮藏期间的生理特性变化。结果显示,黑头病造成采后‘翠香’果实软化迅速,可溶性固形物升高,乙烯释放提前,缩短贮藏周期,感病果果肉中水溶性钙含量显著低于健康果。钙、镁、钾含量及其相关比值与‘翠香’黑头病有一定的相关性,还需要进一步研究。

关键词：黑头病 ‘翠香’ 猕猴桃生理特性

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

苹果液泡铁离子转运蛋白基因家族的鉴定与表达分析

引用

园艺学报 2021年第2期48卷 205-218页

作者：杨亚明丁毓端陈丽娟田雪婷殷伟杰彭红慧杜薇梁丽平任小林西北农林科技大学园艺学院陕西杨凌712100

液泡铁离子转运蛋白(vacuolar iron transporter,VIT)参与铁的储存和运输,对植物光合作用、固氮、呼吸、DNA和激素合成有重要作用。本研究中鉴定了苹果全基因组的9个VIT基因,通过系统发育分析其进化关系,表明苹果VIT蛋白与拟南芥进化关... 详细信息

液泡铁离子转运蛋白(vacuolar iron transporter,VIT)参与铁的储存和运输,对植物光合作用、固氮、呼吸、DNA和激素合成有重要作用。本研究中鉴定了苹果全基因组的9个VIT基因,通过系统发育分析其进化关系,表明苹果VIT蛋白与拟南芥进化关系较近。染色体定位显示9个VIT基因分布在7条染色体上,VIT基因的启动子区域富含光反应、植物激素调控和启动子相关顺式作用元件。表达谱分析显示VIT基因在花和果实中的表达量相对较高。此外,对MdVIT家族进行蛋白理化性质、String互作网络和基因结构等分析,表明苹果VIT家族扩增的主要因素是串联重复和片段复制,与苹果VIT蛋白互作的主要蛋白是阳离子共转运蛋白(XP_008372221.1和XP_008383418.1)。qRT-PCR分析结果表明:在2000μmol·L^(-1)FeSO_(4)·7H_(2)O(高铁)处理的‘M26’苹果砧木苗中,苹果VIT家族基因在不同组织的表达中存在差异,6 h后,MdVIT4在茎中的相对表达量最高,是对照的237倍。24 h后,MdVIT1和MdVIT2在叶片中的相对表达量与对照相比呈现上调表达,分别是对照的6.6倍和12.0倍。综上,基因MdVIT1、MdVIT2和MdVIT4可能与植物耐铁胁迫有密切关系,为苹果逆境胁迫机制研究提供参考。

关键词：苹果全基因组液泡铁离子转运蛋白 VIT基因基因表达

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

课程思政视角下以案例库建设为抓手提升“采后生理”课程授课教师教学能力的研究

引用

农产品加工 2020年第23期 108-110页

作者：刘翠华任小林丁毓端吕燕荣何玲西北农林科技大学园艺学院陕西杨凌712100

在“大思政”与教育信息化环境下,结合西北农林科技大学园艺专业“采后生理”教学实践中的情况,挖掘具有思政内涵的案例,并建设了相应的案例库。系统总结提升教师与学生思政水平的途径与方法,以达到专业思政的目的。

关键词：采后生理课程思政案例教学法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plasma perturbations in the coexisting environment of VLF transmitter emission, lightning strokes and seismic activity

引用

Science China(Technological Sciences) 2018年第5期61卷 678-686页

作者： TAO Dan LIU WenLong MA yuduan School of Space and Environment Beihang University

We analyzed plasma perturbations occurring in the coexisting environment of powerful VLF transmitter emission, intense lightning strokes and strong seismic activity during pregnant period. The results suggest that anomalous electron bursts with energy dispersion in the range of ~100–350 keV, forming the "wisp" signature, are due to cyclotron resonance of electrons with monochromatic waves from the powerful NWC VLF transmitters during nighttime. The intense broad band VLF emissions (up-going 0+ whistlers) are observed while the DEMETER satellite goes through the region of intense thunderstorm activities at mid-latitudes. However, the effects of intense lightning activity and pregnant earthquake have little impact on this kind of stable energy-dispersed electron structures, despite the fact that they are presumably two primary reasons for the particle precipitation in the ionosphere. The case studied here provides us a valuable opportunity to address the various sources triggering the anomalous plasma perturbations in the ionosphere.

关键词： plasma perturbation electron precipitation cyclotron resonance VLF transmitter lightning seismic activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differences between onset times of bursty bulk flows (BBFs) of two Cluster satellites in the magnetotail

引用

Science China(Technological Sciences) 2012年第5期55卷 1178-1183页

作者： CAO JinBin WANG ZhiQiang MA yuduan CAI CunLin Space Science Institute School of AstronauticsBeihang UniversityBeijing 100191China Key Laboratory for Space Weather/CSSAR Beijing 100190China Graduate University Chinese Academy of SciencesBeijing 100080China

This paper, using the dataset of BBFs （bursty bulk flows） observed by two Cluster satellites C1 and C4, studies the difference between onset times of BBFs observed by C1 and C4. It is found that the onset time differences of most of BBFs observed by Cl and C4 are smaller than 60 s. The average onset time difference of BBFs of CI and C4 is 68.5 s. The probabilities of onset time difference of BBFs of C1 and C4 larger than 30, 60, 90 and 120 s are respectively 55%, 35%, 27% and 23%. The largest onset time difference of BBFs of C1 and C4 decreases with the increase of earthward component of maximum velocities of BBFs. The onset time difference of BBFs of C1 and C4 results from the velocity inhomogeneity inside the flow channel of BBF, which may be produced in propagation path and/or in source region of BBFs. Such a wide range of onset time difference of BBFs suggests that the velocity inhomogeneity inside the flow channel of BBF is various. These results are very important to the current study of substorm research based on THEMIS data because they indicate that it is impossible to determine the onset time of BBF with a single satellite.

关键词： bursty bulk flows (BBFs) onset time difference substorm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：