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Elite,transformable haploid inducers in maize

The Crop Journal 2024年第1期12卷 314-319页

作者： Brent Delzer Dawei Liang David Szwerdszarf Isadora Rodriguez Gonzalo Mardones Sivamani Elumalai Francine Johnson Samson Nalapalli Rachel Egger Erin Burch Kerry Meier Juan Wei Xiujuan Zhang Huaping Gui Huaibing Jin Huan Guo Kun Yu yubo liu Becky Breitinger Ana Poets Jason Nichols Wan Shi David Skibbe Qiudeng Que Timothy Kelliher Seeds Development Syngenta SeedsJanesvilleWI 53546USA Seeds Research Syngenta Biotechnology China Co.Ltd.Beijing 102206China Centro de Investigación de Cultivos Arica Syngenta R&DArica 1000000Chile Syngenta S.A. Proyecto la SoledadGraneros 2880000Chile Seeds Research Syngenta Crop Protection LLCRTPNC 27709USA Seeds Development Syngenta SeedsSlaterIA 50244USA

The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent selection.For HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors.Leveraging marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid identification.We show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors.Finally,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers.The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.

关键词： Zea mays L Doubled haploids Transformation Genome editing QTL

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Machine learning noise exposure detection of rail transit drivers using heart rate variability

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Transportation Safety and Environment 2024年第2期6卷 122-132页

作者： Zhiqiang Sun Haiyue liu yubo Jiao Chenyang Zhang Fang Xu Chaozhe Jiang Xiaozhuo Yu Gang Wu School of Transportation and Logistics Southwest Jiaotong UniversityChengdu 611756China Lanzhou Rail Transit Co. Ltd.Lanzhou 730000China Sichuan Tourism University Chengdu 610100China PagerDuty Inc.905 King StWTorontoON M6K 3G9Canada

Previous studies have found that drivers’physiological conditions can deteriorate under noise conditions,which poses a potential hazard when driving.As a result,it is crucial to identify the status of drivers when exposed to different noises.However,such explo-rations are rarely discussed with short-term physiological indicators,especially for rail transit drivers.In this study,an experiment involving 42 railway transit drivers was conducted with a driving simulator to assess the impact of noise on drivers’physiological responses.Considering the individuals’heterogeneity,this study introduced drivers’noise annoyance to measure their self-noise-adaption.The variances of drivers’heart rate variability(HRV)along with different noise adaptions are explored when exposed to different noise conditions.Several machine learning approaches(support vector machine,K-nearest neighbour and random forest)were then used to classify their physiological status under different noise conditions according to the HRV and drivers’self-noise adaptions.Results indicate that the volume of traffic noise negatively affects drivers’performance in their routines.Drivers with different noise adaptions but exposed to a fixed noise were found with discrepant HRV,demonstrating that noise adaption is highly associated with drivers’physiological status under noises.It is also found that noise adaption inclusion could raise the accuracy of classifications.Overall,the random forests classifier performed the best in identifying the physiological status when exposed to noise conditions for drivers with different noise adaptions.

关键词： noise exposure detection noise adaption heart rate variability(HRV) machining learning simulator experiment

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A review of lithium extraction from natural resources

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International Journal of Minerals,Metallurgy and Materials 2023年第2期30卷 209-224页

作者： yubo liu Baozhong Ma Yingwei Lü Chengyan Wang Yongqiang Chen State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Beijing Key Laboratory of Green Recovery and Extraction of Rare and Precious Metals University of Science and Technology BeijingBeijing 100083China

Lithium is considered to be the most important energy metal of the 21st century.Because of the development trend of global electrification,the consumption of lithium has increased significantly over the last decade,and it is foreseeable that its demand will continue to increase for a long time.Limited by the total amount of lithium on the market,lithium extraction from natural resources is still the first choice for the rapid development of emerging industries.This paper reviews the recent technological developments in the extraction of lithium from natural resources.Existing methods are summarized by the main resources,such as spodumene,lepidolite,and brine.The advantages and disadvantages of each method are compared.Finally,reasonable suggestions are proposed for the development of lithium extraction from natural resources based on the understanding of existing methods.This review provides a reference for the research,development,optimization,and industrial application of future processes.

关键词： lithium extraction spodumene lepidolite brine

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Natural ventilation driven by a restricted heat source elevated to different levels

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Building Simulation 2022年第2期15卷 281-289页

作者： Changqing Yang Wenhao Luo Angui Li Xiaopan Gao Lunfei Che Lingmin Qiao Teng Gao yubo liu School of Building Services Science and Engineering Xi'an University of Architecture and TechnologyXi'anShaanxi 710055China China Railway Siyuan Survey and Design Group Co. Ltd.WuhanHubei 430063China Artificial Environment Control Technology Innovation Center of Xi'an University of Architecture and Technology-Yantai YantaiShandong 264006China

The thermal buoyancy generated by the difference in air density in a building can drive hot-pressed natural ventilation,which is an energy-efficient means of ventilation used to obtain higher air quality.Therefore,the effect of a single-point heat source with limited sides at different heights on stratified flow was studied in a naturally ventilated room in this paper.Based on the classical plume diffusion law of an independent point heat source and the mirroring principle,a calculation model of the thermal stratification height with a restricted source elevated to different levels was derived and validated.The quantitative effects of the heat source height from the floor,the effective opening area and other factors on the natural ventilation of hot pressure were analyzed.A threshold X_(T)for the separation between a point source and a sidewall was defined to estimate whether the thermal plume was independent or restricted by a sidewall.And a method for calculating the threshold xt was obtained.This research can provide a reference basis for designing natural ventilation for buildings with a restricted heat source at different levels to achieve a desired indoor environment.

关键词： stratification height natural ventilation thermal plume restricted heat source different levels

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Prediction of specific spoilage organisms in smoked chicken legs with modified atmosphere packaging at 4 ℃ using multivariate statistical analysis

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Food Science and Human Wellness 2024年

作者： Qiang Wang yubo Zhao Yumeng Sui Qian Chen Zhiming Dong Qian liu Baohua Kong Ligang Qin College of Animal Science and Technology Northeast Agricultural University College of Food Science Northeast Agricultural University

The present study monitored bacterial succession， physicochemical properties， and volatile organic compounds (VOCs) changes in smoked chicken legs with modified atmosphere packaging (MAP， 60% CO2and 40% N2) during a 25-day storage period at 4 ℃. After 15 days of storage， Serratia proteamaculans and Pseudomonas fragi became the predominant bacteria. Furthermore， physicochemical properties changed significantly， as evidenced by an increase in thiobarbituric acid reactive substances and b* (yellowness) value， and a decrease in hardness. A total of 65 VOCs were identified during storage. Correlation between bacterial succession and quality indicators (including VOCs and physicochemical properties) allowed the identification of 26 core dominant bacteria， including S. proteamaculans， Psychrobacter alimentarius， Pseudomonas putide， and Pseudomonas poae， which were positively related to spoilage VOCs (e.g.， 1-octen-3-ol， 1-pentanol， and 3-methyl-1-butanol) and could be defined as specific spoilage organisms (SSOs). The results of this study provide a systematic approach to predict SSOs in smoked chicken legs during storage， which can also provide a basis for product safety.

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自信与尊严:马克思劳动幸福观的当代阐释

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道德与文明 2024年第2期 84-93页

作者：刘禹伯吉林大学东北亚学院吉林长春130012

幸福是人类的永恒追求和社会发展的不竭动力,古往今来哲学家们一直探讨幸福是什么,马克思在前人思想精华基础上,指明了幸福是生活在现实社会中的全部人的幸福。马克思从现实的、能动的人的层面发掘了导致人们不幸的诱因,即在劳动过程中... 详细信息

幸福是人类的永恒追求和社会发展的不竭动力,古往今来哲学家们一直探讨幸福是什么,马克思在前人思想精华基础上,指明了幸福是生活在现实社会中的全部人的幸福。马克思从现实的、能动的人的层面发掘了导致人们不幸的诱因,即在劳动过程中,创造者与享受者的背离、脱轨,使劳动者丧失了信心与尊严。因此,只有以劳动为手段,使劳动成果重归劳动者本身,树立劳动者的自信与尊严,才能实现真正的幸福。在马克思的劳动幸福观中,自由劳动作为内在本质,是人类实现幸福的根本途径,自信与尊严作为其重要表征,在个人与社会的发展进程中得以彰显。与此同时,马克思劳动幸福观为生活在新时代的人们追求幸福指明方向,充分展现了马克思劳动幸福观的现实价值。

关键词：幸福自信尊严马克思劳动幸福观

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Installing hydrogen bonds as a general strategy to control viscosity sensitivity of molecular rotor fluorophores Special Collection:Aggregation-Induced Processes and Functions

Aggregate

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Aggregate 2024年第1期5卷 367-376页

作者： Baoxing Shen Lihua liu yubo Huang Jichun Wu Huan Feng Yu liu He Huang Xin Zhang Department of Chemistry Research Center for Industries of the FutureWestlake UniversityHangzhouChina Westlake Laboratory of Life Sciences and Biomedicine HangzhouChina School of Food Science and Pharmaceutical Engineering Nanjing Normal UniversityNanjingChina CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

Molecular rotor-based fluorophores(RBFs)activate fluorescence upon increase of micro-viscosity,thus bearing a broad application promise in many fields.However,it remains a challenge to control how fluorescence of RBFs responds to viscosity changes.Herein,we demonstrate that the formation and regulation of intramolecular hydrogen bonds in the excited state of RBFs could modulate their rotational barrier,leading to a rational control of how their fluorescence can be activated by micro-viscosity.Based on this strategy,a series of RBFs were developed based on 4-hydroxybenzylidene-imidazolinone(HBI)that span a wide range of viscosity sensitivity.Combined with the AggTag method that we previously reported,the varying viscosity sensitivity and emission spectra of these probes enabled a dualcolor imaging strategy that detects both protein oligomers and aggregates during the multistep aggregation process of proteins in live cells.In summary,our work indicates that installing intracellular excited state hydrogen bonds to RBFs allows for a rational control of rotational barrier,thus allow for a fine tune of their viscosity sensitivity.Beyond RBFs,we envision similar strategies can be applied to control the fluorogenic behavior of a large group of fluorophores whose emission is dependent on excited state rotational motion,including aggregation-induced emission fluorophores.

关键词： fluorescent probe intramolecular hydrogen bond protein aggregation rotor-based fluorophores viscosity sensitivity

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Mapping of Purple Gene in Spears of Asparagus(Asparagus officinalis L.)

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Agricultural Biotechnology 2024年第1期13卷 5-8页

作者： Tao liu Wenlai REN Yuqin LIANG yubo WANG Wei liu Xing WANG Yanpo CAO School of Landscape and Ecological Engineering Hebei University of EngineeringHandan 056000China Wen-an County Agriculture and Rural Bureau Langfang 065800China Institute of Cash Crops Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China

[Objectives]This study was conducted to find out regulatory genes related to purple in spears of asparagus(Asparagus officinalis L.).[Methods]The stable asparagus inbred line JX1513-5(the base of the spear is purple)and JLV1718-7(the base of the spear is green)were used as parents to study the genetic law of purple/green traits in their offspring.[Results]The results showed that the purple in the basal part of asparagus spear was controlled by a pair of alleles,and purple was dominant over green.The F 2 segregation population was resequenced by the bulk segregation analysis(BSA)method,and the purple trait in the basal part of asparagus spear was located in the interval of 24.51-25.08 Mb on Chr07 chromosome,which included 47 genes.According to the annotation information,three candidate genes were screened out:LOC109849403,LOC109849430 and LOC109849442.The candidate genes were verified by real-time fluorescence quantitative PCR(qRT-PCR),and finally LOC109849442 was obtained as the candidate gene for controlling the purple/green trait in the basal part of asparagus spear.[Conclusions]This study lays a foundation for the breeding of new asparagus varieties and molecular marker-assisted breeding.

关键词： Asparagus Anthocyanins Gene mapping Purple

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Zn掺杂提升AgSnSbSe3热电性能的研究

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华中科技大学学报(自然科学版) 2024年第6期52卷 124-129页

作者：刘海强罗裕波范淑媛华中科技大学材料科学与工程学院材料成型与模具技术国家重点实验室湖北师范大学物理与电子科学学院

通过高温熔炼的方法制备掺Zn的Ag Sn Sb Se3，分析了Ag Sn Sb Se3掺Zn后得到的Ag Sn Sb1-xZnxSe3（x=0.01,0.02,0.03,0.04）的相组成、形貌、元素组成和热电性能的关系，并利用COMSOL Multiphysic仿真模拟了热电优值（ZT值）最高的Ag Sn... 详细信息

通过高温熔炼的方法制备掺Zn的Ag Sn Sb Se3，分析了Ag Sn Sb Se3掺Zn后得到的Ag Sn Sb1-xZnxSe3（x=0.01,0.02,0.03,0.04）的相组成、形貌、元素组成和热电性能的关系，并利用COMSOL Multiphysic仿真模拟了热电优值（ZT值）最高的Ag Sn Sb0.98Zn0.02Se3的发电性能．研究表明：所有样品均为高对称的岩盐Na Cl结构，Zn掺杂可在样品中形成纳米第二相，一定量的Zn掺杂可协同优化Ag Sn Sb Se3的电声输运性能，样品Ag Sn Sb0.98Zn0.02Se3获得最高热电优值0.86@823 K．当温差为460 K时，获得的最大发电功率为11.372 m W，发电效率为5%，对Ag Sn Sb Se3掺杂外来原子可提升其热电性能．

关键词：掺杂高温熔炼热电材料电声输运性能热电性能

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矿物组分识别与智能解释在不同岩性之间的信息共享与迁移学习

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地学前缘 2024年第4期31卷 95-111页

作者：刘烨韩雨伯朱文瑞西安石油大学计算机学院陕西西安710065

在地球科学领域,岩石微观观测数据的采集过程繁琐且效率低下,这不仅增加了研究成本,降低了可靠性,同时也限制了数据的开源共享。此外,由于岩性的多样性和观测手段的差异,单一数据集的规模通常较小,这对于依赖大规模数据集的深度学习框... 详细信息

在地球科学领域,岩石微观观测数据的采集过程繁琐且效率低下,这不仅增加了研究成本,降低了可靠性,同时也限制了数据的开源共享。此外,由于岩性的多样性和观测手段的差异,单一数据集的规模通常较小,这对于依赖大规模数据集的深度学习框架而言是一大挑战。为此,本研究探索迁移学习如何促进不同岩性间的信息共享,并通过此机制提高矿物组分识别与智能解释任务的模型性能。通过采集不同区域、岩性、矿物组分和偏光模式下的铸体薄片样本,本文深入研究了深度学习模型在不同观测对象和手段下的迁移学习机制,并聚焦于探索地质信息的深层表征。研究成果不但揭示了迁移学习在促进地质学领域信息共享与模型性能提升中的关键作用,还为自动化和智能化地质认识融合奠定了基础。实验结果显示,通过迁移学习,本文模型在智能解释任务中的准确率显著提高,从53.3%提高至98.73%,而在矿物组分识别任务中,准确率也实现了近10%的提升。这些成果证明了迁移学习在地质学领域内解决实际问题和提高模型泛化能力、性能和稳定性方面的巨大潜力。

关键词：迁移学习薄片矿物组分识别薄片图像智能解释地质认识融合

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