检索结果-南通市图书馆

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

水生生物外泌体功能的研究进展

生物学杂志 2024年第2期41卷 97-102页

作者：蔡承建蒋家超罗萍王立章张媛媛谷秀君中国矿业大学环境与测绘学院徐州221116

外泌体是一种由多种细胞分泌的30~150 nm的小囊泡,在组织细胞生理和病理情况下皆可持续分泌,存在于多种体液当中,通过转运蛋白质、脂质、核酸等方式影响或改变受体细胞的行为,在细胞间通讯、免疫调节等方面发挥重要作用。以水生生物外... 详细信息

外泌体是一种由多种细胞分泌的30~150 nm的小囊泡,在组织细胞生理和病理情况下皆可持续分泌,存在于多种体液当中,通过转运蛋白质、脂质、核酸等方式影响或改变受体细胞的行为,在细胞间通讯、免疫调节等方面发挥重要作用。以水生生物外泌体为切入点,对外泌体的组成特征以及不同种类水生生物的外泌体功能进行综述,并对影响生物外泌体分泌的环境因素进行分析,探讨外泌体释放量与环境因素的关系,旨在为水生生物外泌体和环境保护提供新的研究思路。

关键词：外泌体水生生物功能环境保护

SIN3B promotes integrin αV subunit gene transcription and cell migration of hepatocellular carcinoma

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2019年第5期11卷 421-432页

作者： Qianqian cai yuanyuan Liu ping Zhu Chunlang kang Heyang Xu Bing Qi Rong Wang Yiwei Dong Xing Zhong Wu Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Fudan University. Key Lab of Glycoconjugate Research Ministry of Public Health Shanghai 200032 China Zhejiang Provincial People's Hospital Hangzhou 310053 China

Paired amphipathic helix protein (SIN3B) is a transcription corepressor for many genes. Here we show a different regulation mechanism of integrin aV gene expression by SIN3B in human hepatocellular carcinoma (HCC). We first observed a close relationship between Integrin aV and SIN3B expressions in HCC patients and tumor cell lines with different metastatic potentials. Overexpression of SIN3B significantly accelerated the cell migration rate of SMMC-7721, but failed when integrin αV expression was silenced. Interestingly, SIN3B stimulated integrin aV subunit promoter activity only in the presence of sulfatide. Importantly, SIN3B was identified in the complex with sulfatide by mass spectrometry. Fat blot assay indicated that SIN3B specifically interacted with sulfatide. Molecular modeling suggested that sulfatide induced the conformational change of SIN3B from compacted α-helices to a relaxed β-sheet in PAH2 domain. The data of immunoprecipitation and ChIP assay indicated that altered SIN3B lost the binding affinity with MAD1 and HDAC2, which reduced the recruitment of HDAC2 on integrin aV gene promoter and prevented the deacetylation of the histone 3. In conclusion, this study demonstrated that SIN3B promoted the transcriptional activation of the integrin αV subunit gene promoter by reducing interaction with HDAC2.

关键词： paired amphipathic helix protein ITGAV sulfatide HCC histone deacetylation

Neurotoxicity due to dimethylamine borane poisoning via skin absorption:a case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Emergency Medicine 2022年第6期13卷 500-503页

作者： Guang-cai Yu Ya-qian Li Tian-zi Jian Bao-tian kan Si-qi Cui ping Han Xiang-dong Jian Department of Poisoning and Occupational Diseases Emergency MedicineQilu Hospital of Shandong UniversityCheeloo College of MedicineShandong UniversityJinan 250012China Shandong University of Traditional Chinese Medicine Jinan 250012China Department of Geriatric Medicine Qilu Hospital of Shandong UniversitySchool of NursingCheeloo College of MedicineShandong UniversityJinan 250012China School of Public Health Cheeloo College of MedicineShandong UniversityJinan 250012China

Dear editor,Dimethylamine borane(DMAB,CAS no.74-94-2),also called boron-dimethylamine complex,is a white crystalline *** has an amine odour under mild conditions.[1]As an excellent reducing agent,DMAB is widely used in the semiconductor industry,pharmaceutical manufacturing,[2]oligomer and polymer production,and hydrogen storage.[3]Acute DMAB exposure may cause eye or respiratory irritation,throat discomfort,vomiting,diarrhoea,and pulmonary oedema.[1,4]Isolated reports indicated that DMAB exposure causes acute cerebellar oedema and peripheral neuropathy.[1,5,6]Herein,we report a case of DMAB poisoning via dermal absorption in which the central and peripheral nervous systems were the primary sites of toxic *** highlight the use of brain magnetic resonance imaging(MRI)and electromyography(EMG)for evaluation after exposure.

关键词： conditions. absorption boron

Schiff-base silver nanocomplexes formation on natural biopolymer coated mesoporous silica contributed to the improved curative effect on infectious microbes

在线全文

学校读者我要写书评

暂无评论

Nano Research 2021年第8期14卷 2735-2748页

作者： Ling cai Yanqiang Huang yuanyuan Duan Qiao Liu Qilan Xu Jia Jia Jianming Wang Qian Tong Peipei Luo Yujie Wen Luming Peng Qian Wu Xudong Hang Huijun Jiang ping Zhu Yanmei Yang Boshen Zhou Liping Zeng Hongkai Bi Jin Chen Center for Global Health The Key Laboratory of Modern ToxicologyMinistry of EducationSchool of Public HealthNanjing Medical UniversityNanjing 211166China Department of Pathogen Biology Jiangsu Key Laboratory of Pathogen BiologyNanjing Medical UniversityNanjing 211166China Research Center for the Prevention and Treatment of Drug Resistant Microbial Infecting Youjiang Medical University for Nationalities Baise 533000China The Laboratory Center for Basic Medical Sciences Nanjing Medical UniversityNanjing 211166China Key Laboratory of Mesoscopic Chemistry of MOE and Collaborative Innovation Center of Chemistry for Life Sciences School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210023China School of Pharmacy Nanjing Medical UniversityNanjing 211166China Department of Gastroenterology Sir Run Run HospitalNanjing Medical UniversityNanjing 211166China Key Laboratory of Antibody Technique of National Health Commission Nanjing Medical UniversityNanjing 211166China

Infectious microbes that spread easily in healthcare facilities remain as the severe threat for the public health,especially among immunocompromised *** the intricate problem of dramatic increase in resistance to common biocides,the development of safe and efficient biocide formulated agents to alleviate drug resistance is highly *** this study,Schiff-base ligands were successfully formed on natural biopolymer of epsilon-poly-L-lysine(ε-PL)decorated aldehyde functionalized mesoporous silica SBA-15(CHO-SBA-15)for the selective coordination of silver ions,which was affirmed by various physicochemical *** the identified broad-spectrum antibacterial activities,the as-prepared Schiff-base silver nanocomplex(CHO-SBA-15/ε-PL/Ag,CLA-1)exhibited an improved inhibitory effect on infectious pathogen growth typified by Escherichia coli and Staphylococcus aureus in comparison with two control silver complexes without Schiff-base conjugates,SBA-15/ε-PL/Ag and CHO-SBA-15/Ag,*** addition,CLA-1 remarkably inhibited the growth of Mycobacterium tuberculosis due to the excellent antimicrobial activity of silver ***,CLA-1 kills Candida albicans cells,inhibits biofilm formation,and eliminates preformed biofilms,with no development of resistance during continuous serial *** antifungal activity is connected to disruption of bacterial cell membranes and increased levels of intracellular reactive oxygen *** mouse models of multidrug-resistant *** infection,CLA-1 exhibited efficient in vivo fungicidal efficacy superior to two antifungal drugs,amphotericin B and ***,CLA-1 treatment induces negligible toxicity against normal tissues with ***,this study reveals the pivotal role of the molecular design of Schiff-base silver nanocomplex formation on biopolymer surface-functionalized silica mesopores as a green and efficient nanoplatform to tackle infectious microbes.

关键词： biopolymer drug delivery mesoporous silica silver nanoparticles antimicrobial drug resistance

Aptamer-Anchored Rubrene-Loaded Organic Nanoprobes for Cancer Cell Targeting and Imaging

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CCS Chemistry 2019年第3期1卷 251-260页

作者： yuanyuan Li Chuansheng Li Tao Jiang Lihua Zhou Pengfei Zhang Ryan T.K.Kwok Jacky Wing Yip Lam ping Gong Lintao cai Ben Zhong Tang Department of Chemistry The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced StudyDepartment of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyClear Water BayKowloonChina HKUST Shenzhen Research Institute Shenzhen 518057China Guangdong Key Laboratory of Nanomedicine CAS Key Laboratory of Health InformaticsShenzhen Bioactive Materials Engineering Lab for MedicineInstitute of Biomedicine and Biotechnology.Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Department of Pharmaceutical Sciences Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Dongguan Key Laboratory of Drug Design and Formulation Technology Key Laboratory for NanomedicineGuangdong Medical UniversityDongguan 523808China Center for Aggregation-Induced Emission NSFC Center for Luminescence from Molecular AggregatesSCUT-HKUST Joint Research LaboratoryState Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhou 510640China

Fluorescence imaging has become an indispensable technique in cancer research because it can reveal informative molecular,cellular,anatomical,and func-tional *** of advanced fluores-cent probes with superior sensitivity and biological selectivity for fluorescence imaging is thus *** move forward in this direction,we developed an easy self-assembly method for fabricating apta-mer-anchored rubrene-loaded organic fluorescent *** aptamer-modified organic nanop-robes integrated the best features of the organic light-emitting materials and the aptamers,thus endowing them with excellent cell-targeting capabil-ity,high stability,and good *** using this general method,a variety of biocompatible and highly bright organic fluorescent nanoprobes based on novel organic light-emitting materials with specific recognition could be easily constructed for real-time biosensing and long-term biomedical imaging.

关键词： aptamer organic nanoprobes fluores-cence cancer imaging biomedicine

改进的还原偶氮光度法同时快速测定水中硝基苯和苯胺

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国环境监测 2015年第3期31卷 150-154页

作者：邢书才田衎鞠平环境保护部标准样品研究所国家环境保护污染物计量和标准样品研究重点实验室北京100029

对测定方法进行了改进，建立了用还原．偶氮分光光度法同时快速测定水中硝基苯和苯胺的分析方法。根据实验原理，对样品前处理装置进行了改进，实验步骤得到了简化，避免了样品溶液过滤转移过程造成样品损失。使样品前处理实验效率提高... 详细信息

对测定方法进行了改进，建立了用还原．偶氮分光光度法同时快速测定水中硝基苯和苯胺的分析方法。根据实验原理，对样品前处理装置进行了改进，实验步骤得到了简化，避免了样品溶液过滤转移过程造成样品损失。使样品前处理实验效率提高了90％以上；改进后的测定方法不仅可快速测定样品中硝基苯，还可同时测定样品中苯胺；达到了用一种分析方法同时测定样品中两种污染物的目的。方法精密度测定结果，相对标准偏差（n=6，RSD）为1．9％～3．2％；对硝基苯、苯胺混合标准样品以及实际废水样品进行了测定，并与原测定方法及苯胺国标测定方法进行了比对实验：测定结果具有一致性。加标实验回收率为96％～101％，表明方法准确可靠，可用于水和废水中硝基苯或硝基苯和苯胺的同时测定。

关键词：还原偶氮光度法硝基苯苯胺同时测定

A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2022年第4期3卷 37-53页

作者： Congcong Jiang Miaomiao Lei Yu Guo Guangqi Gao Lijie Shi Yanlong Jin Yu cai Axel Himmelbach Shenghui Zhou Qiang He Xuefeng Yao Jinhong kan Georg Haberer Fengying Duan Lihui Li Jun Liu Jing Zhang Manuel Spannagl Chunming Liu Nils Stein Zongyun Feng Martin Mascher ping Yang Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijingChina College of Agronomy Sichuan Agricultural UniversityChengduChina Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) SeelandGermany Institute of Botany Chinese Academy of SciencesBeijingChina Plant Genome and Systems Biology(PGSB) Helmholtz Center MunichGerman Research Center for Environmental HealthNeuherbergGermany

Barley is a diploid species with a genome smaller than those of other members of the Triticeae tribe,making it an attractive model for genetic studies in Triticeae *** recent development of barley genomics has created a need for a high-throughput platform to identify genetically uniform mutants for gene function *** this study,we report an ethyl methanesulfonate(EMS)-mutagenized population consisting of 8525M_(3) lines in the barley landrace“Hatiexi”(HTX),which we complement with a high-quality de novo assembly of a reference genome for this *** mutation rate within the population ranged from 1.51 to 4.09 mutations per megabase,depending on the treatment dosage of EMS and the mutation discrimination platform used for genotype *** implemented a three-dimensional DNA pooling strategy combined with multiplexed amplicon sequencing to create a highly efficient and cost-effective TILLING(targeting induced locus lesion in genomes)platform in *** were successfully identified from 72 mixed amplicons within a DNA pool containing 64 individual mutants and from 56 mixed amplicons within a pool containing 144 *** discovered abundant allelic mutants for dozens of genes,including the barley Green Revolution contributor gene Brassinosteroid insensitive 1(BRI1).As a proof of concept,we rapidly determined the causal gene responsible for a chlorotic mutant by following the MutMap strategy,demonstrating the value of this resource to support forward and reverse genetic studies in barley.

关键词： barley mutagenesis TILLING amplicon-seq genetics

Equilibrium electron beam parameters of the High Energy Photon Source

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

辐射探测技术与方法(英文) 2023年第2期7卷 279-287页

作者： Haisheng Xu cai Meng Yuemei Peng Saike Tian Na Wang Xiaohao Cui Chongchong Du Zhe Duan yuanyuan Guo ping He Xiyang Huang Daheng Ji Hongfei Ji Yi Jiao Jingyi Li Nan Li Xiaoyu Li Xiaohan Lu Pengfei Liang Weimin Pan Huamin Qu Bin Wang Jiuqing Wang yuanyuan Wei Jinyu Wan Gang Xu Fang Yan Chenghui Yu Sen Yue Xiang Zhang Yaliang Zhao Key Laboratory of Particle Acceleration Physics and Technology Institute of High Energy PhysicsChinese Academy of Sciences19B Yuquan RoadShijingshan DistrictBeijing 100049China University of the Chinese Academy of Sciences No.19(A)Yuquan RoadShijingshan DistrictBeijing 100049China

Purpose The physics design of the High Energy Photon Source(HEPS)was finished after many times of ***,the typical equilibrium electron beam parameters corresponding to the proposed two baseline operation modes in the baseline design of HEPS are *** To compute the equilibrium parameters of the electron beam,the lattice parameters,RF parameters,and the parameters of the insertion devices(IDs)were determined ***,it is more precise to use the full-current electron beam parameters in the estimations of the performance of the synchrotron ***,not only the single-particle dynamics but also the current-dependent collective effects need to be considered in the computations of the full-current,equilibrium parameters of the electron *** analytic computations and multi-particle tracking simulations were carried *** The full-current,equilibrium parameters of the electron beams in the HEPS storage ring are presented in this ***,the main beam parameters in the injector(the booster and the LINAC),corresponding to the two baseline operation modes of the storage ring,are also *** The typical electron beam parameters corresponding to the two baseline operation modes are given in detail in this paper.

关键词： High Energy Photon Source Electron beam parameters

基于拟三元等温图对宁东煤灰高温熔融流动特性的研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

石油学报（石油加工） 2023年第1期39卷 134-141页

作者：阚浩勇李平陈财牛艳青宁夏大学化学化工学院省部共建煤炭高效利用与绿色化工国家重点实验室宁夏银川750021 西安交通大学能源与动力工程学院动力工程多相流国家重点实验室陕西西安710049

在气流床气化炉的实际运行中,经常需要寻求最优的进料煤调配方案以调控煤灰的熔融流动特性。借助SiO_(2)-Al_(2)O_(3)-AAEM(CaO+MgO+Na_(2)O+K_(2)O)拟三元等温图,分析了不同组成的宁东煤灰熔融特性,并结合X-射线衍射(XRD)探究了灰在熔... 详细信息

在气流床气化炉的实际运行中,经常需要寻求最优的进料煤调配方案以调控煤灰的熔融流动特性。借助SiO_(2)-Al_(2)O_(3)-AAEM(CaO+MgO+Na_(2)O+K_(2)O)拟三元等温图,分析了不同组成的宁东煤灰熔融特性,并结合X-射线衍射(XRD)探究了灰在熔化过程中的矿物质转化规律。发现熔融温度在拟三元等温图中呈现U型分布。当SiO_(2)、Al_(2)O_(3)和AAEM质量分数分别为37.5%、25.0%和37.5%时,灰的熔融温度达到最低,流动温度比宁东煤灰降低40℃。对灰熔化过程中矿物质转化分析发现,熔化过程中石英与氧化钙会发生矿物质转化反应生成低熔点的钙黄长石和长石,对其熔化具有明显的促进作用;对灰熔化后形成液态熔渣的黏-温特性曲线分析发现,该比例下灰样品同时具有最低的临界黏性温度(T_(CV)=1107℃)和最低的黏性流动活化能(E_(a)=282.86 kJ/mol),可有效降低气化炉的操作温度。研究表明,拟三元等温图对于气化原料配煤方案可起到一定的指导作用。

关键词：宁东煤灰熔融特性流动特性矿物质拟三元等温图