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经鼻持续气道正压通气与丙种球蛋白在小儿重症肺炎合并呼吸衰竭中的应用研究

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系统医学 2024年第13期9卷 97-100页

作者：鲁彦凤王艳丽车媛媛曹县人民医院呼吸与危重症四病区山东菏泽274400 曹县人民医院儿科山东菏泽274400

目的探讨重症肺炎(Severecase Pneumonia,SP)合并呼吸衰竭(Respiratory Failure,RF)患儿采用静脉注射丙种球蛋白(Intravenous Immunoglobulin,IVIG)与经鼻持续气道正压通气(Nasal Continuous Positive Airway Pressure Ventilation,NCPAP)联合治疗的效果。方法目的选取2022年8月—2023年7月期间曹县人民医院收治的SP合并RF患儿88例,根据治疗方法不同分组,对照组44例患儿采用NCPAP治疗,在此基础上研究组44例联合IVIG治疗。针对两组疗效、症状及体征消退时间、炎症因子与不良反应进行评价。结果研究组治疗总有效率(95.45%)比对照组的79.55%高,差异有统计学意义(χ^(2)=5.091,P0.05)。结论SP合并RF患儿采用NCPAP联合IVIG治疗效果确切,可以促进症状与体征快速消退,抑制炎症反应。

关键词：经鼻持续气道正压通气丙种球蛋白小儿重症肺炎呼吸衰竭

Thermal-annealing-regulated plasmonic enhanced fluorescence platform enables accurate detection of antigen/antibody against infectious diseases

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Nano Research 2023年第2期16卷 3215-3223页

作者： Ying Yue Jingjie Nan yuanyuan che Hongqin Xu Weihong Sun Feiran Zhang Lei Wang Wei Xu Junqi Niu Shoujun Zhu Junhu Zhang Bai Yang State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012China Joint Laboratory of Opto-Functional Theranostics in Medicine and chemistry The First Hospital of Jilin UniversityChangchun 130021China Department of Laboratory Medicine The First Hospital of Jilin UniversityChangchun 130021China Department of Hepatology Center of Infectious Diseases and Pathogen BiologyThe First Hospital of Jilin UniversityChangchun 130021China

Plasmonic enhanced fluorescence(PEF)technology is a powerful strategy to improve the sensitivity of immunofluorescence microarrays(IFMA),however,current approaches to constructing PEF platforms are either expensive/time-consuming or reliant on specialized ***,we develop a completely alternative approach relying on a two-step protocol that includes the self-assembly of gold nanoparticles(GNPs)at the water–oil interface and subsequent annealing-assisted regulation of gold *** optimized thermal-annealing GNPs(TA-GNP)platform generates adequate hot spots,and thus produces high-density electromagnetic coupling,eventually enabling 240-fold fluorescence enhancement of probed dyes in the near-infrared *** clinical detection of human samples,TA-GNP provides super-high sensitivity and low detection limits for both hepatitis B surface antigen and SARS-CoV-2 binding antibody,coupled with a much-improved detection dynamic range up to six orders of *** fast detection,high sensitivity,and low detection limit,TA-GNP could not only substantially improve the outcomes of IFMA-based precision medicine but also find applications in fields of proteomic research and clinical pathology.

关键词： plasmonic enhanced fluorescence electromagnetic coupling SARS-CoV-2 antibodies hepatitis B surface antigen immunofluorescence microarrays

基于虚拟试验场技术的汽车悬架缓冲块虚拟调校

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汽车工程师 2024年第1期 37-42页

作者：蔡剑吴泽勋车媛媛贾宝光李太梅王德远重庆睿蓝汽车研究院有限公司重庆401122

基于虚拟试验场(VPG)技术,搭建整车动力学模型及试验场路面模型,针对某车型开展了车身疲劳耐久仿真,针对仿真中发现的车身耐久风险点多且损伤值大的问题进行分析,发现悬架缓冲块载荷过大是车身损伤偏大的直接原因,基于VPG技术对缓冲块... 详细信息

基于虚拟试验场(VPG)技术,搭建整车动力学模型及试验场路面模型,针对某车型开展了车身疲劳耐久仿真,针对仿真中发现的车身耐久风险点多且损伤值大的问题进行分析,发现悬架缓冲块载荷过大是车身损伤偏大的直接原因,基于VPG技术对缓冲块进行了虚拟调校,通过增大缓冲块上跳行程减少了缓冲块处于压缩极限的频次,进而降低了车身疲劳损伤。道路耐久试验验证结果表明,优化方案增强了车身耐久性能,并提升了整车舒适性。

关键词：悬架缓冲块虚拟试验场车身耐久虚拟调校试验场验证

Novel variants of SYNGAP1 associated epileptic encephalopathy: two cases report and literature review

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Acta Epileptologica 2023年第1期6卷 56-63页

作者： Xingying Zeng Yong chen Xiongying Yu yuanyuan che Hui chen Zhaoshi Yi Jie Qin Jianmin Zhong Molecular Diagnostic Laboratory Children’s Hospital of Jiangxi ProvinceNanchang 330006China Department of Neurology Children’s Hospital of Jiangxi ProvinceNanchang 330006China

Background SYNGAP1 is a signifcant genetic risk factor for global developmental delay,autism spectrum disorder,and epileptic *** novo loss-of-function variants in this gene cause a neurodevelopmental disorder,for example,early-onset and drug-refractory *** report two children with global developmental delay and epileptic encephalopathy,which are caused by SYNGAP1 gene novel mutations,and drug treatment is *** presentation We report a boy and a girl presented with global developmental delay when they were young babies;as they grew up,cognitive impairment and social-communication disorder became more and more prominent;unfortunately,the patients developed into various seizure types,including eyelid myoclonia,myoclonic and absences when the boy was 1 year 8 mouths old and the girl was 3 years *** two patients were found two previously unknown mutations by high throughput sequencing[c.3271_c.3272insT;(p.L1091L fs^(*)62),c.2515A>T(p.K839^(*))]in exon 15 of the SYNGAP in the *** sequencing confrmed the heterozygous nature,and neither of their parents carried the same *** girl treated with valproic acid and prednisone became seizure-free,and valproic acid and levetiracetam combined with clonazepam were infuential in the *** The global developmental delay and epileptic encephalopathy of the children were probably due to the pathogenic mutation of the SYNGAP1 gene,and prednisone and clonazepam may be efective in achieving seizure-free.

关键词： SYNGAP1 Epilepsy Prednisone Clonazepam Case report

Comparative Experiment of Ground Cucumber Varieties for Open Field Cultivation

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Agricultural Biotechnology 2018年第3期7卷 30-31页

作者： Meiguang BI yuanyuan che Lizhi YU Yantai Research Institute of China Agricultural University Yantai 264670 China

Seven ground cucumber varieties were cultivated in open field for the comparative experiment. The results showed that Texuan cucumber and Yantai ground cucumber had good commodity characters,good taste and high yield,and their output value exceeded 16 000 yuan/666. 7 m2,so they are more suitable for open cultivation in Yantai region. The next ones are Meiyu Diguawang and Aweishi ground cucumber.

关键词： Open field cultivation Ground cucumber Variety Comparative experiment

Photosynthetic co-culture system of algae and human umbilical vein endothelial cells: The effect on alleviating hypoxia and hypoxia/reoxygenation injury

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BIOSURFACE AND BIOTRIBOLOGY 2024年第2期10卷 76-88页

作者： Lin, Donghu chen, yuanyuan Tao, Xinyu che, Xin Li, Shiyu cheng, Shiyu Qu, Shuxin Southwest Jiaotong Univ Inst Biomed Engn Coll Med Chengdu Sichuan Peoples R China Southwest Jiaotong Univ Sch Mat Sci & Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China Southwest Jiaotong Univ Inst Biomed Engn Coll Med Chengdu 610031 Sichuan Peoples R China Southwest Jiaotong Univ Sch Life Sci & Engn Chengdu Sichuan Peoples R China

It is a developed photosynthetic co-culture system to alleviate the hypoxia and hypoxia/reoxygenation (H/R)-injured human umbilical vein endothelial cells (HUVECs). The algae, Chlorella vulgaris, were encapsulated to slow their growth while not affecting the photosynthetic oxygen-producing capacity by Layer-by-layer (LbL) using gelatin and sodium alginate as the positive and negative charges materials, respectively. Then, the photosynthetic co-culture system of HUVECs and self-oxygenating alginate hydrogel (Algae-gel) was constructed in which the optimal ratios between algae and HUVECs were 5:1 and 20:1 for a 2D or 3D co-cultured manner, respectively. It indicated that the 3D co-cultured manner of HUVECs needed more O2 by the production of algae than it did in a 2D co-cultured manner. The co-cultured Algae-gel could alleviate hypoxia and the oxidative stress injury of hypoxia and hypoxia/reoxygenation (H/R)-treated HUVECs in the proliferation, intracellular ROS and cellular migratory ability. In addition, the Algae-gel could downregulate the expression of hypoxia-inducible factors 1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) of hypoxia and H/R-injured HUVECs due to the improvement of hypoxia and H/R injury. This photosynthetic co-culture system could offer a promising approach for repairing hypoxia and H/R-injured cells or tissue by providing safe and stable O2.

关键词： biomaterials biomedical application bionic design regenerative medicine self-assembly tissue engineering OXYGEN BIOMATERIALS PHYSIOLOGY

Tissue Culture and Rapid Propagation of Pedicels of Hemerocallis hybrida

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Agricultural Biotechnology 2012年第3期1卷 20-22页

作者： Guanglin PENG Xiuchen XIN yuanyuan che Zhifen ZHANG Shuyi SHI Yantai Institute China Agricultural University

[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS ＋ 2.0 - 3.0 mg/L 6-BA ＋ 0.2 - 0.3 mg/L NAA, MS ＋ 1.0 - 2.0 mg/L 6-BA ＋ 0.1 - 0.2 mg/L NAA, MS and 1/2MS ＋ 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.

关键词： Hemerocallis hybrida Pedicel in vitro culture Rapid propagation

Five-year surveillance of antimicrobial resistance in Pseudomonas aeruginosa isolated in a tertiary hospital in China

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Five-year surveillance of antimicrobial resistance in Pseudo...

Effects of increased human tumor necrosis factor-like molecule 1A expression in peripheral blood of children with acute Guillain-Barre syndrome on interferon-gamma secretion

第三届中国临床微生物学大会暨微生物学与免疫学论坛

作者： Jiancheng Xu Dandan Gao yuanyuan che Qi Zhou Department of Clinical Laboratory The First Bethune Hospital of Jilin University Department of Pediatrics The First Bethune Hospital of Jilin University

Objective This study was to investigate the antimicrobial resistance of Pseudomonas aeruginosa isolated in 5 consecutive years in a tertiary hospital in *** Disk diffusion test was used to study the antimicrobial *** data were analyzed by WHONET 5 software according to Clinical and Laboratory Standards Institute（CLSI）.Results Most of 1932 strains of *** were collected from sputum 940（48.7%）,secretions and pus 315（16.3%）,urine 299（15.5%） during the past 5 *** resistance rates of *** for piperacillin,ticarcillin/clavulanic acid,ampicillin/sulbactam,trimethoprim/sulfamethoxazole,cefotaxime,ceftriaxone and gentamicin were almost more than 50%.The resistance rates for imipenem,meropenem,piperacillin/tazobactam,cefoperazone/sulbactam,ceftazidime,cefepime,amikacin ciprofloxacin,levofloxacin and gatifloxacin were shown a relative low,however,they were almost more than 30%with a substantial increase during 5 years in the tertiary *** antimicrobial resistance of *** had increased in recent 5 *** The change of the antimicrobial resistance should be investigated in order to direct rational drug usage in the tertiary hospital and prevent bacterial strain of drug resistance from being transmitted.

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Neural Regeneration Research 2009年第10期4卷 763-768页

作者： Libin Yang Shulei Li Yan Tan Shufen Xu Xiumei Duan Yanqiu Fang Lihua Liu yuanyuan che Lei Liu Liwei Zhou Department of Pediatrics First Hospital Jilin University Changchun 130021 Jilin Province China Department of Histology and Embryology Bethune Medical School Jilin University Changchun 130021 Jilin Province China Central Laboratory First Hospital Jilin University Changchun 130021 Jilin Province China

BACKGROUND： Human tumor necrosis factor-like molecule 1A （hTL1A） is a strong T helper cell type 1 （Thl） co-stimulator. Guillain-Barre syndrome （GBS） is an autoimmune disorder of the nervous system, which is mediated by Thl cells. OBJECTIVE： To determine hTL1A expression in peripheral blood T lymphocytes of acute GBS children and the effects of hTL1A on secretion of interferon-γ. DESIGN, TIME AND SETTING： A randomized, controlled, neuroimmunological in vitro study was performed at the Central Laboratory of First Hospital of Jilin University, China from November 2005 to November 2007. MATERIALS： Venous blood samples were obtained from 6 healthy donors, aged 6-12 years （all routine blood examination items were normal）, and 6 additional children with acute GBS, aged 6-12 years. The GBS children fell ill within 1 week and were not treated with hormones or immunoglobulin Purified recombinant human soluble tumor necrosis factor-like molecule 1A （rhsTL1A, 1 mg/mL, relative molecular mass 22 000, 6× His tag, soluble form） was supplied by the Central Laboratory of First Hospital of Jilin University, China. METHODS： Peripheral blood mononuclear cells were isolated from healthy donors using the standard Ficoll gradient centrifugation and were incubated in 96-well culture plates. The cells were assigned to the following groups： control （2 μg/mL phytohemagglutinin）, 2μg/mL phytohemagglutinin ＋ 25, 100 and 400 ng/mL rhsTL1A. T cell proliferation was quantified using the tritiated thymidine （3H-TdR） method. Serum interferon-γ levels in acute GBS children were detected by enzyme-linked immunosorbent assay （ELISA）. The ratio of hTL1A-positive T cells to CD3-positive T cells in peripheral blood of acute GBS children was determined using flow cytometry. Following in vitro pre-activation of peripheral blood mononuclear cells by 2 μg/mL phytohemagglutinin, the peripheral blood mononuclear cells were treated with 400 ng/mL exogenous rhsTLIA. Finally, peripheral blood mononuclear cell-secr

关键词： human tumor necrosis factor-like molecule 1 A cell proliferation Guillain-Barre syndrome interferon-γ