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A micro amperometric immunosensor for detection of human immunoglobulin

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Science in China(Series F) 2006年第3期49卷 397-408页

作者： XU yuanyuan XIA Shanhong bian Chao CHEN Shaofeng State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of SciencesBeijing 100080 China

A novel amperometric immunosensor based on the micro electromechanical systems （MEMS） technology, using protein A and self-assembled monolayers （SAMs） for the orientation-controlled immobilization of antibodies, has been developed. Using MEMS technology, an ＂Au, Pt, Pt＂ three-microelectrode system enclosed in a SU-8 micro pool was fabricated. Employing SAMs, a monolayer of protein A was immobilized on the cysteamine modified Au electrode to achieve the orientation-controlled immobilization of the human immunoglobulin （HIgG） antibody. The immunosensor aimed at low unit cost, small dimension, high level of integration and the prospect of a biosensor system-on-a-chip. Cyclic voltammetry and chronoamperometry were conducted to characterize the immunosensor. Compared with the traditional immunosensor using bulky gold electrode or screen-printed electrode and the procedure directly binding protein A to electrode for immobilization of antibodies, it had attractive advantages, such as miniaturization, compatibility with CMOS technology, fast response （30 s）, broad linear range （50-400 pg/L） and low detection limit （10 pg/L） for HIgG. In addition, this immunosensor was easy to be designed into micro array and to realize the simultaneously multi-parameter detection.

关键词： amperometric immunosensor micro electromechanical systems (MEMS) self-assembled monolay-ers (SAMs) protein A orientation-controlled immobilization biosensor system-on-a-chip.

A Micro Amperometric Immunosensor Based on MEMS

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稀有金属材料与工程 2006年第A03期35卷 339-341页

作者： Chao bian yuanyuan Xu Hongguang Sun Shaofeng Chen Shanhong Xia State Key Lab of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100080 China

A micro amperometric immunosensor with the sensitive area of only 1mm^2 was fabricated on silicon using the technique of Micro-Electro-Mechanical Systems (MEMS).A double exposure of SU-8 photoresist process was developed to create both the sensitive pool and reaction *** was immobilized via cross-linking with glutaraldehyde on the sensitive area of the electrode surface,which was electropolymerized with polypyrrole *** immunosensor was characterized by detection of human immunoglobulin G (HIgG).The immunosensor displayed a good linear response to HIgG concentrations between 5ng/ml and 255ng/ml and demonstrated a fast response time of 3 minutes.

关键词： enzyme amperometric immunosensor MEMS polypyrrole IgG

Potato protease inhibitors,a functional food material with antioxidant and anticancer potential

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Food Science and Human Wellness 2023年第5期12卷 1762-1771页

作者： Yaotong Liu yuanyuan bian Yan Bai Siqi Yu Yuxuan Tian Jie Li Suhong Li Tuoping Li College of Food Science Shenyang Agricultural UniversityShenyang 110866China College of Grain Science and Technology Shenyang Normal UniversityShenyang 110034China College of Pharmaceutical Science Liaoning UniversityShenyang 110036China

Potato protease inhibitors(PPIs),as the main component of potato protein isolate,have good safety,nutrition and great market *** antioxidant and anticancer properties of PPIs were evaluated with cellbased biological *** results showed that when the concentration of PPIs was 5 mg/mL,the peroxyl radical scavenging value was(2119±204)mg VCE/100 g,and the cellular antioxidant activity values were(45.83±3.5)(no PBS wash)and(33.25±4.4)μmol QE/100 g(PBS wash).Cells pretreated with PPIs could significantly prevent the oxidative damage induced by H_(2)O_(2),inhibit the morphological changes of cells and maintain the ***,PPIs had selective anti-proliferative effects on GIST882 cells(IC50=(10.53±3.87)mg/mL)and demonstrated potent inhibition of the growth,migration and invasion of cancer *** findings provide a scientific basis for PPIs as promising candidates for functional foods to aid in the prevention of oxidative damage and cancer.

关键词： Potato protease inhibitors Antioxidant activity Cytoprotection Anticancer activity

Pterostilbene antagonizes homocysteine-induced oxidative stress,apoptosis and lipid deposition in vascular endothelial cells

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Food Science and Human Wellness 2023年第5期12卷 1683-1692页

作者： Qiao Jiang Li Wang Xu Si yuanyuan bian Weijia Zhang Huijun Cui Hailong Gui Ye Zhang Bin Li Dehong Tan College of Food Science Shenyang Agricultural UniversityShenyang 110866China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China

Hyperhomocysteinemia(HHcy)causes oxidative stress, induces apoptosis, and leads to damage to the vascular endothelium is the starting point of atherosclerosis. Pterostilbene(Pte)has been reported to have antioxidant and anti-apoptotic effects under various pathological conditions. The purpose of this study was to explore whether Pte can inhibit the oxidative stress and apoptosis of vascular endothelium induced by homocysteine(Hcy)and to explain the possible mechanism by which it occurs. The results showed that 20 μmol/L Pte significantly reduced the accumulation of reactive oxygen species, malondialdehyde, and lipids in cells induced by Hcy and promoted the activities of superoxide dismutase and catalase. The Hoechst 33342/PI staining assay showed that Pte antagonized Hcy-induced apoptosis. Pte inhibited Hcy-induced Akt dephosphorylation, increased p53, and decreased the Bcl-2/Bax ratio and caspase-9/caspase-3 activation in a dose-dependent manner. LY294002 pretreatment partially reversed the protective effect of Pte by blocking the PI3K/Akt pathway. Moreover, Pte reduced lipid deposition in human umbilical vein endothelial cells(HUVECs). This study proposes that Pte can inhibit Hcy-induced oxidative stress and apoptosis of HUVECs, and the PI3K/Akt/p53 signaling pathway of apoptosis was revealed. These results suggest that Pte exhibits significant potential for dealing with HHcy-induced vascular endothelial injury, such as atherosclerosis.

关键词： Pterostilbene Hyperhomocysteinemia Methionine Polyphenols Atherosclerosis

Sexual spores in edible mushroom: bioactive components, discharge mechanisms and effects on fruiting bodies quality

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Food Science and Human Wellness 2023年第6期12卷 2111-2123页

作者： Yunting Li Yao Feng Yuling Shang Heran Xu Rongrong Xia Zhenshan Hou Song Pan Li Li yuanyuan bian Jiayi Zhu Zijian Wang Guang Xin College of Food Science Shenyang Agricultural UniversityShenyang 110866China Shenyang Agricultural University Library Shenyang 110866China

Edible mushroom sexual spores have been gaining more interest due to their bioactive components and *** discharge(SD)is an important factor affecting the quality of edible *** this review,the bioactive nutrients of sexual spores of edible mushrooms were summarized,the SD mechanism was described,and the relationship between postharvest SD and the quality of edible mushrooms was *** contain various bioactive nutrients that are benefi cial to the human *** mushrooms can actively discharge spores in a process affected by light,relative humidity,and *** storage,the physiological metabolism of spore-bearing gill tissue is vigorous,promoting the release of postharvest spores and changing the nutritional value of fruiting *** flavor of the fruiting bodies also varied signifi cantly during *** mushroom sexual spores have the potential to become new raw materials for functional food and medical *** on the effect of the mechanism of SD on the quality of edible mushrooms and the development of SD regulation technology may be a new trend in the quality control of edible mushrooms,which will promote the development of the edible mushroom industry.

关键词： Edible mushroom Sexual spores Bioactive component Discharge mechanism Postharvest quality

基于公园城市建设导向的街道绿视率评价方法探究

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中国园林 2021年第S2期37卷 11-15页

作者：卞媛媛上海市园林设计研究总院有限公司上海200031

随着"公园城市"这一理念在成都市的提出,全国的规划师不断努力探索其发展路径与具体的评价标准。以建设公园城市为目标,从国土空间规划至生态环境建设和设施服务运营等方面均有待提升。针对街道评价指标的缺失,通过绿视率与街道活力指... 详细信息

随着"公园城市"这一理念在成都市的提出,全国的规划师不断努力探索其发展路径与具体的评价标准。以建设公园城市为目标,从国土空间规划至生态环境建设和设施服务运营等方面均有待提升。针对街道评价指标的缺失,通过绿视率与街道活力指标的量化,客观评价街道生态环境及设施服务水平。首先,通过街道实景照片及兴趣点(POI)数据的获取,计算得出杨浦区街道绿视率水平及城市街道活力度。其次,基于分析结果,客观评价街道现状特征并明确存在问题。最后,针对性地提出相应优化策略,为城市决策者提供更好的技术支持。

关键词：风景园林绿视率街道兴趣点城市活力

Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期 308-316页

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong yuanyuan Shi Bin Hong Xiaoying bian Wen Liu State Key Laboratory of Chemical Biology Shanghai Institute of Organic Chemistry University of Chinese Academy of SciencesChinese Academy of Sciences State Key Laboratory of Microbial Technology Shandong University National Health Commission (NHC) Key Laboratory of Biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science & Peking Union Medical College

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM) proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual members. Many of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding reactions. In the biosynthetic pathway of chuangxinmycin(CXM), a unique indole alkaloid antibiotic with potent anti-infective activity, functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process, which, however, remained to be reconstituted biochemically. We here report the dissection of this enzymatic process, which requires the incorporation of Cxm8, a Cbl-dependent radical SAM protein, with Cxm9, a DUF5825family protein that shares no homology to any proteins of known functions. Cxm8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner, achieving CXM and a recently identified, C3-dimethylated congener. Detailed biochemical characterization,isotope labeling, structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase activity. This is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

关键词：

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新城区景观风貌规划探析

中外建筑 2021年第10期 100-102页

作者：卞媛媛上海市园林设计研究总院有限公司

景观风貌规划具有进一步突出空间特色、彰显区域特征的重要作用。为细化、分解和落实总体规划及控制性详细规划明确的相关引导性要求,本文以上海市虹桥主城前湾地区为研究范围,探析如何依据宏观定位、现状条件、历史资源等因素,确定与... 详细信息

景观风貌规划具有进一步突出空间特色、彰显区域特征的重要作用。为细化、分解和落实总体规划及控制性详细规划明确的相关引导性要求,本文以上海市虹桥主城前湾地区为研究范围,探析如何依据宏观定位、现状条件、历史资源等因素,确定与之适配的风貌引导要点与管控要求,从而切实有助于塑造并提升虹桥主城前湾地区的整体形象。

关键词：风貌规划公共空间色彩岸线地标

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无痛分娩改善母婴结局的效果分析

系统医学 2022年第14期7卷 166-169页

作者：卞媛媛睢宁县妇幼保健院妇产科江苏徐州221200

目的分析在产妇分娩过程中采取无痛分娩技术对母婴结局的影响。方法选择2021年1-12月睢宁县妇幼保健院收治的初产妇60名为研究对象,采取随机数表法分为两组,各30名;对照组采取常规分娩,观察组采取无痛分娩。对比两组产程时间、临床指... 详细信息

目的分析在产妇分娩过程中采取无痛分娩技术对母婴结局的影响。方法选择2021年1-12月睢宁县妇幼保健院收治的初产妇60名为研究对象,采取随机数表法分为两组,各30名;对照组采取常规分娩,观察组采取无痛分娩。对比两组产程时间、临床指标以及母婴结局。结果观察组第一、二、三及总产程时间分别为(310.00±100.10)、(51.20±34.40)、(9.11±1.39)、(370.33±102.17)min,均短于对照组的(572.83±147.33)、(85.57±49.34)、(10.40±2.73)、(667.80±166.44)min,差异有统计学意义(t=8.082、3.130、2.306、8.343,P<0.05)。观察组产妇各临床指标(VAS评分、产时出血量、泌乳时间、下床时间、住院时间、分娩后5 min Apgar评分)均优于对照组,差异有统计学意义(t=17.759、11.688、3.947、7.799、8.862、2.441、14.146,P<0.05)。观察组不良结局发生率为6.67%,低于对照组的26.67%,差异有统计学意义(χ^(2)=4.320,P<0.05)。结论在产妇分娩过程中,采取无痛分娩技术可有效缩短产妇产程及术后恢复时间,并有效改善产妇疼痛程度、各项临床指标以及母婴结局,值得推广。

关键词：无痛分娩技术母婴结局产程时间疼痛程度 Apgar评分

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