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稀有金属材料与工程 2006年第A03期35卷 270-273页

作者： Nongyue He yuanying zhang Jinan zhang State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China Jiangsu Cancer Hospital Nanjing 210007 China Research Institute of Cardiovascular Disease First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (FHCM).A microarray was fabricated to screen mutations in exons 3,5,7,and 8 in cTnI *** were designed for the PCR (polymerase chain reaction) to amplify the target DNA fragments from fresh blood *** order to simplify the PCR process,multiplex PCR technology was investigated in *** concentration of Mg^(2+) played an important role in multiplex PCR process,a properly low concentration of Mg^(2+) submitted a better speciality of PCR *** speciality was also favored when the annealing temperature was reasonably enhanced and 64℃is the optimal annealing temperature for the multiplex PCR *** applying the fabricated gene-chip to detect the target fragments from PCR mixture,the signal intensity sequence is in accordance with that from theoretic estimate.

关键词： hypertrophic cardiomyopathy(HCM) gene-chip multiplex PCR mutation hybridization

Automated screening of primary cell-based aptamers for targeting and therapy of pancreatic cancer

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Chinese Chemical Letters 2024年第2期35卷 300-304页

作者： Zhukang Guo Baijiang Jin Yile Fang Lian Jin Song Li Yan Deng Zhu Chen Hui Chen yuanying zhang Rabia Usman Nongyue He State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Department of Molecular Biology Jiangsu Cancer HospitalNanjing 210009China

Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased *** address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this ***, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular ***, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.

关键词： Aptamer Cell-SELEX Automatic instrument Combination therapy Pancreatic cancer

Effects of Mg on C and N Metabolism of Soybean at Different Nitrogen Supplying Levels

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Journal of Northeast Agricultural University(English Edition) 2006年第1期13卷 31-35页

作者： LANG Man LIU yuanying PENG Xianlong zhang Wenzhao Resources and Environmental Science College Northeast Agricultural University Harbin Heilongjiang 150030 PRC

A pot experiment was conducted to study the effects of magnesium on carbon and nitrogen metabolism of soybean at different nitrogen supplying levels. The results showed that the effects of magnesium at low nitrogen rate on N content, soluble protein, soluble sugar contents were not alike at different growth stage, although nodule dry weights raised, the yield and protein content of seeds decreased, however, the oil content was improved. The application of magnesium at medium and high nitrogen supplying levels promoted the uptake of N effectively, increased the soluble protein and soluble sugar contents, but the nodule dry weights of application magnesium at medium nitrogen supplying level decreased and the yield increased only a little despite the improved quality. Application of magnesium at high nitrogen supplying level raised nodule dry weights and soybean yield significantly, the quality of seeds was also improved.

关键词： soybean magnesium(Mg) nitrogen supplying carbon and nitrogen metabolism

Totalhipreplacementforcongenitaldislocationofthehipinelderlypatients

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吉林大学学报(医学版) 1994年第1期 41-44页

作者： Duan Desheng zhang yuanying,Jiang Hongzhi,Gao Zhongli,Wedge J H(Department of Orthopaedic Surgery, Third Teaching Hospital)

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关键词： hip dislocation congenital/SU aged

Collinear holographic data storage technologies

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Opto-Electronic Advances 2020年第3期3卷 25-32页

作者： Xiao Lin Jinpeng Liu Jianying Hao Kun Wang yuanying zhang Hui Li Hideyoshi Horimai Xiaodi Tan College of Photonic and Electronic Engineering Fujian Normal UniversityFuzhou 350117China School of Optics and Photonics Beijing Institute of TechnologyBeijing 100081China HolyMine Corporation 2032-2-301 OokaNumazuShizuoka 410-0022Japan

In the era of information explosion,the demand of data storage is increased *** data storage technology is one of the most promising next-generation data storage technologies due to its high storage density,fast data transfer rate,long data life time and less energy *** holographic data storage technology is the typical solution of the holographic data storage technology which owns a more compact,compatible and practical *** paper gives a brief review of holographic data storage,introduces collinear holographic data storage technology and discusses phase modulation technology being used in the holographic data storage system to achieve higher storage density and higher data transfer rate.

关键词： holographic data storage collinear holography high density phase modulation

Linear polarization holography

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Opto-Electronic Science 2022年第2期1卷 10-29页

作者： Jinyu Wang Xiaodi Tan Peiliang Qi Chenhao Wu Lu Huang Xianmiao Xu Zhiyun Huang Lili Zhu yuanying zhang Xiao Lin Jinliang Zang Kazuo Kuroda College of Photonic and Electronic Engineering Fujian Normal UniversityFuzhou 350108China Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal UniversityFuzhou 350108China Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education Fujian Normal UniversityFuzhou 350108China Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application Fujian Normal UniversityFuzhou 350108China National Institute of Metrology Chaoyang DistrictBeijing 100029China Institute of Industrial Science The University of TokyoMeguro-kuTokyo 153-8505Japan

Polarization holography is a newly researched field,that has gained traction with the development of tensor *** primarily focuses on the interaction between polarization waves and photosensitive *** extraordinary capabil-ities in modulating the amplitude,phase,and polarization of light have resulted in several new applications,such as holo-graphic storage technology,multichannel polarization multiplexing,vector beams,and optical functional *** this paper,fundamental research on polarization holography with linear polarized wave,a component of the theory of polariz-ation holography,has been ***,the effect of various polarization changes on the linear and nonlinear po-larization characteristics of reconstructed wave under continuous exposure and during holographic recording and recon-struction have been focused *** polarization modulation realized using these polarization characteristics exhibits unusual functionalities,rendering polarization holography as an attractive research topic in many fields of *** paper aims to provide readers with new insights and broaden the application of polarization holography in more sci-entific and technological research fields.

关键词： polarization holography tensor theory polarization modulation linear and nonlinear polarization characteristics

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基于遗传编程的吸附床传热状态预测

工程热物理学报 2023年第6期44卷 1720-1725页

作者：张元英冯黛丽冯妍卉张欣欣北京科技大学能源与环境工程学院北京100083 北京科技大学冶金工业节能减排北京市重点实验室北京100083

脱硝吸附塔是烟气处理系统中的重要环节。塔内的热交换受到多方因素的非线性影响,给准确快速的评估吸附剂换热状态带来巨大挑战。为解决此问题,使用数值模拟与均一介质方法,对一种插管式吸附塔换热进行了模拟与多元遗传编程回归,获得了... 详细信息

脱硝吸附塔是烟气处理系统中的重要环节。塔内的热交换受到多方因素的非线性影响,给准确快速的评估吸附剂换热状态带来巨大挑战。为解决此问题,使用数值模拟与均一介质方法,对一种插管式吸附塔换热进行了模拟与多元遗传编程回归,获得了白盒表达式并验证模型准确性。分析了因变量、遗传编程参数等设定对回归的影响。最终结果表明使用遗传编程的回归效果优异,在工业生产与探究变量物理关系中,具有强大的应用潜力。

关键词：吸附床换热遗传编程误差分析机器学习符号回归

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保偏光纤耦合型铌酸锂晶体工频强电场传感器

仪器仪表学报 2021年第6期42卷 272-278页

作者：张元英张家洪李英娜雷鸿毅昆明理工大学信息工程与自动化学院昆明650500 云南省计算机应用技术重点实验室昆明650500

随着我国特高压技术的发展,工频强电场的测量变得越来越重要。为了适应工频强电场时域测量的需要,基于Pockels效应,设计并研制了一种保偏光纤耦合型铌酸锂晶体工频强电场传感器。通过保偏光纤准直器耦合进行光输入和光输出,避免了起偏器... 详细信息

随着我国特高压技术的发展,工频强电场的测量变得越来越重要。为了适应工频强电场时域测量的需要,基于Pockels效应,设计并研制了一种保偏光纤耦合型铌酸锂晶体工频强电场传感器。通过保偏光纤准直器耦合进行光输入和光输出,避免了起偏器、1/4波片等分离光学元件的使用,将传感器的光耦合效率提高到了66.7%,并且通过将输入保偏光纤准直器前端1/2拍长的保偏光纤扭转90°,可将传感器的静态工作点设置为π/2,从而使传感器工作在近线性区。使用工频强电场对传感器进行测试得出:传感器的线性可测范围为10.5~527 kV/m,线性相关系数为0.9986,线性动态范围为34 dB。相比于现有的铌酸锂晶体强电场传感器,研制的传感器具有结构简单紧凑、耦合效率高等优点,为工频强电场的时域测量提供了一种新的方案。

关键词：强电场测量 Pockels效应保偏光纤耦合工频强电场传感器静态工作点

番茄Lehsp23.8启动子的热诱导表达调控序列鉴定

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生物工程学报 2009年第6期25卷 826-831页

作者：伊淑莹翟静徐华张媛英泰山医学院基础医学部泰安271000 泰安市中心医院高压氧科泰安271000

番茄线粒体小分子热激蛋白(Lehsp23.8)启动子是典型的热诱导启动子。为了研究热激条件下该启动子的调控序列,本研究将不同长度的Lehsp23.8启动子序列与gus基因融合,构建5′缺失植物表达载体。然后用农杆菌介导法转化烟草,PCR及Southern ... 详细信息

番茄线粒体小分子热激蛋白(Lehsp23.8)启动子是典型的热诱导启动子。为了研究热激条件下该启动子的调控序列,本研究将不同长度的Lehsp23.8启动子序列与gus基因融合,构建5′缺失植物表达载体。然后用农杆菌介导法转化烟草,PCR及Southern blotting结果表明融合基因已经整合到烟草基因组中。GUS组织化学染色结果表明:不同长度Lehsp23.8启动子转基因植株热激处理后,在幼苗根、茎、叶以及花和果实中均表现出GUS活性,只是染色强弱有差异。叶片中GUS荧光活性测定结果表明:在热激处理条件下,565bp的Lehsp23.8启动子介导的GUS表达最强;而255bp的Lehsp23.8启动子介导的GUS表达最弱。说明Lehsp23.8启动子中255bp的序列即能满足该启动子的热激表达,-565bp～-255bp之间存在明显的增强子元件,而-871bp～-565bp之间的片段具有一定的抑制作用。

关键词：番茄线粒体小分子热激蛋白启动子缺失 GUS

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论《武林怡老会诗集》的尊礼与怡情

铜仁学院学报 2019年第6期21卷 110-118页

作者：张媛颖上海大学文学院

《武林怡老会诗集》是明代万历年间张瀚等人唱和诗歌的结集。该集不但呈现了明代官员致仕后的唱和活动,还体现了明人观念中的尊礼思想,展现了唱和者丰富的情感世界。尊礼方面主要体现为:诗集编排上的遵从"四序"、画像编排上的以齿为序... 详细信息

《武林怡老会诗集》是明代万历年间张瀚等人唱和诗歌的结集。该集不但呈现了明代官员致仕后的唱和活动,还体现了明人观念中的尊礼思想,展现了唱和者丰富的情感世界。尊礼方面主要体现为:诗集编排上的遵从"四序"、画像编排上的以齿为序、主会形式上的迭为主宾和雅集活动时的有礼有节。这与张瀚本人对礼的坚守、诸老对前代结社的效仿及教化乡邦的观念有关。怡情方面,怡老会诗歌绘耄耋之容、祈寿考之愿,兴桑榆之叹、行及时之乐,情契释道、希冀立言不朽。和前代怡老会相比,其所怡之情更趋平和、更同质化。在情礼关系上,怡老会既崇礼、又怡情,以礼节情,从而使其诗歌呈现出尚雅的特点。明代怡老社团完整保存下社集的不多,《武林怡老会诗集》的文献和文化价值不容忽视。

关键词：《武林怡老会诗集》尊礼怡情尚雅