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Neural Regeneration Research 2011年第29期6卷 2279-2283页

作者： Jianhua Lu yuanxiang tao Xue Yang Weifeng Tu Hao Chen Jiaxiang Xiong Chungui Hu Department of Anesthesiology Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA Guangzhou 510010 Guangdong Province China Department of Anesthesiology and Critical Care Medicine Johns Hopkins University School of Medicine Baltimore Maryland 21205 USA Department of Physiology Third Military Medical University Chongqing 400038 China

Spinal dorsal horn N-Methyl-D-aspartic acid receptor 2B （NR2B） overexpression plays an important role in the production and maintenance of neuropathic pain. Because small interfering RNA （siRNA） can inhibit NR2B expression, siRNA may provide a novel approach to treat neuropathic pain and possibly nerve injury. However, an efficient and safe vector for NR2B siRNA has not been discovered. This study shows that a water soluble lipopolymer （WSLP） comprised of low molecular weight polyethyleneimine （PEI） and cholesterol can deliver siRNA targeting NR2B for the treatment of neuropathic pain. Results show that intrathecal injection of WSLP/siRNA complexes for 3 days inhibit NR2B gene expression with reductions in mRNA and protein levels by 59% and 54%, respectively, compared with control rats （P 〈 0.01）. Injection of WSLP complexed with scrambled siRNA, or PEI with siRNA did not show this inhibitory effect. Moreover, injection of WSLP/siRNA complexes significantly relieved neuropathic pain at 3, 7, 12, and 21 days, while injection of WSLP with scrambled siRNA or PEI with siRNA did not. These results demonstrate that WSLP can efficiently deliver siRNA targeting NR2B in vivo and relieve neuropathic pain.

关键词： water soluble lipopolymer N-Methyl-D-aspartic acid receptor 2B small interfering RNA peripheral nerve injury neuropathic pain

KDM4A通过下调BMP9促进乳腺癌细胞MDA-MB-231的迁移和侵袭

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中国癌症杂志 2024年第2期34卷 176-184页

作者：陈远香余涛杨诗雨曾涛魏兰张彦重庆医科大学检验医学院临床检验诊断学教育部重点实验室重庆400016

背景与目的:外源性骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)能抑制人乳腺癌的恶性进展,但其在乳腺癌中常异常低表达。本研究拟探索表观遗传修饰的组蛋白赖氨酸特异性去甲基化酶4A(lysine specific demethylase 4A,KDM4A)在... 详细信息

背景与目的:外源性骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)能抑制人乳腺癌的恶性进展,但其在乳腺癌中常异常低表达。本研究拟探索表观遗传修饰的组蛋白赖氨酸特异性去甲基化酶4A(lysine specific demethylase 4A,KDM4A)在乳腺癌中的表达及作用,探究KDM4A与BMP9之间的关系及其可能的调节机制。方法:通过生物信息学方法分析KDM4A在乳腺癌中的表达及其与BMP9之间的关系,采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)和蛋白质印迹法(Western blot)进行验证;采用染色质免疫沉淀(chromatin immunoprecipitation,ChIP)验证KDM4A对BMP9的调控作用,采用RNA稳定性实验及CHX蛋白稳定性实验验证KDM4A对BMP9表达的影响。采用RNA干扰技术及敲低BMP9的腺病毒构建转染KDM4A小干扰RNA(siKDM4A)或感染siBMP9腺病毒(Ad-siBMP9)的外源性重组MDA-MB-231细胞,采用划痕愈合实验、transwell实验分别检测细胞迁移及侵袭能力。结果:生物信息学分析结果表明,KDM4A在乳腺癌中的表达明显高于正常组织,KDM4A与BMP9在乳腺癌中的表达呈负相关关系。RTFQ-PCR及Western blot结果显示,KDM4A在不同乳腺癌细胞系中均高表达,敲低KDM4A可显著上调BMP9。ChIP实验证实,KDM4A可显著富集于BMP9基因启动子区域,降低其组蛋白赖氨酸36号位而不是4号位甲基化水平,从而沉默BMP9表达。RNA稳定性实验及CHX蛋白稳定性实验证实,KDM4A对BMP9的mRNA表达无明显影响,但可影响其蛋白降解。敲低KDM4A后,乳腺癌细胞MDA-MB-231的迁移及侵袭能力均受到明显抑制,而这种作用可被敲低BMP9所部分逆转。结论:KDM4A在乳腺癌组织及乳腺癌细胞MDA-MB-231中高表达,并可通过下调BMP9基因启动子区域组蛋白甲基化水平沉默其表达,以及在蛋白水平而不是mRNA水平影响BMP9稳定性,促进乳腺癌的迁移和侵袭。

关键词：乳腺癌赖氨酸特异性去甲基化酶4A 组蛋白去甲基化骨形态发生蛋白9

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术后持续性疼痛的研究进展

中华麻醉学杂志 2019年第6期39卷 655-659页

作者：田雪陶元祥北京大学人民医院麻醉科 100044 罗格斯大学新泽西州立医学院麻醉科美国纽瓦克07103

术后患者均会出现不同程度的疼痛,多数仅维持数天至几周.然而也有相当一部分患者在伤口痊愈之后仍存在持续性疼痛.虽然目前尚无能被广泛接受的术后持续性疼痛的定义,但是大多数研究者把术后开始出现并持续2~3个月后仍存在的疼痛定义为... 详细信息

术后患者均会出现不同程度的疼痛,多数仅维持数天至几周.然而也有相当一部分患者在伤口痊愈之后仍存在持续性疼痛.虽然目前尚无能被广泛接受的术后持续性疼痛的定义,但是大多数研究者把术后开始出现并持续2~3个月后仍存在的疼痛定义为术后持续性疼痛或术后慢性痛[1].术后持续性疼痛可降低患者术后生活质量[2],显著增加医疗支出和社会负担[3].

关键词：持续性疼痛术后患者术后生活质量社会负担医疗支出慢性痛

ULTRASTRUCTURAL LOCALIZATION OF SUBSTANCE PRECEPTOR AND ITS SYNAPTIC RELATIONSHIP WITH THE PRIMARY AFFERENTS IN THE SUPERFICIAL DORSAL HORN OF THE RAT SPINAL CORD

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解剖学报 1998年第3期 6-9页

作者： tao yuanxiang,Li Yunqing *,Zhao Zhiqi (Shanghai Brain Research Institute,Chinese Academy of Sciences,Shanghai\ 200031,China * Department of Anatomy,The Fourth Military Medical University,Xi'an\ 710032,China)

ＵＬＴＲＡＳＴＲＵＣＴＵＲＡＬＬＯＣＡＬＩＺＡＴＩＯＮＯＦＳＵＢＳＴＡＮＣＥＰＲＥＣＥＰＴＯＲＡＮＤＩＴＳＳＹＮＡＰＴＩＣＲＥＬＡＴＩＯＮＳＨＩＰＷＩＴＨＴＨＥＰＲＩＭＡＲＹＡＦＦＥＲＥＮＴＳＩＮＴＨＥＳＵＰＥＲＦＩ... 详细信息

ＵＬＴＲＡＳＴＲＵＣＴＵＲＡＬＬＯＣＡＬＩＺＡＴＩＯＮＯＦＳＵＢＳＴＡＮＣＥＰＲＥＣＥＰＴＯＲＡＮＤＩＴＳＳＹＮＡＰＴＩＣＲＥＬＡＴＩＯＮＳＨＩＰＷＩＴＨＴＨＥＰＲＩＭＡＲＹＡＦＦＥＲＥＮＴＳＩＮＴＨＥＳＵＰＥＲＦＩＣＩＡＬＤＯＲＳＡＬＨＯＲＮＯＦＴ...

关键词： Substance P receptor Primary afferent Ultrastructure Dorsal horn Spinal cord Rat

腹腔镜下右半结肠癌根治术后胃瘫综合征29例临床分析

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临床外科杂志 2024年第2期32卷 196-198页

作者：李阳刘涛曾立鹏胡元祥武汉脑科医院/长江航运总医院胃肠外科 430010 武汉协和医院肿瘤医院消化肿瘤外科

目的探讨腹腔镜右半结肠癌术后胃瘫综合征(PSG)发生的影响因素。方法 2012年12月~2022年6月我院和武汉协和医院肿瘤医院收治右半结肠癌病人1070例,均行腹腔镜右半结肠完整结肠系膜切除。依据是否发生术后胃瘫分为胃瘫组和无胃瘫组。采... 详细信息

目的探讨腹腔镜右半结肠癌术后胃瘫综合征(PSG)发生的影响因素。方法 2012年12月~2022年6月我院和武汉协和医院肿瘤医院收治右半结肠癌病人1070例,均行腹腔镜右半结肠完整结肠系膜切除。依据是否发生术后胃瘫分为胃瘫组和无胃瘫组。采用单因素分析及多因素Logistic回归分析影响右半结肠切除术后发生PSG的危险因素。结果 1070例病人中,发生胃瘫29例。单因素分析结果显示,年龄、围手术期血糖水平、手术切除范围、手术路径与PGS发生相关(P<0.05)。多因素Logistic分析显示,年龄、围手术期血糖水平高、尾侧入路+联合入路及手术切除范围大是PGS发生的独立影响因素(P<0.05)。结论年龄、围手术期血糖水平高、尾侧入路+联合入路及手术切除范围大是PGS发生的影响因素。

关键词：右半结肠癌术后胃瘫综合征手术方式治疗

Metabolomics mechanism of traditional soy sauce associated with fermentation time

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Food Science and Human Wellness 2022年第2期11卷 297-304页

作者： Li Zhu Siyu He Ying Lu Jianhong Gan Ningping tao Xichang Wang Zaoli Jiang yuanxiang Hong Changhua Xu College of Food Science and Technology Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai 201306China Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai) Ministry of AgricultureShanghai 201306China Department of Pharmacology Yale UniversityNew HavenCT06520US Xiamen Gulong Food Co. Ltd.Xiamen 361000China National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai) Shanghai 201306China

Given that fermentation time has significant impact on quality and flavor of soy sauce,a global understanding of the metabolic mechanism as fermentation time prolonged is essential for producing satisfactory and consistent quality of traditional soy ***,the metabolic compounds changes of soy sauce associated with fermentation time up to 10 years were comprehensively investigated by using Chinese traditional fermented soy sauce(CTFSS)as a *** showed that formaldehyde nitrogen,total soluble nitrogen(TSN),non-salt soluble solids,amino acids,free 5’-nucleotides and volatile compounds in CTFSS changed obviously during ***,glutamic acid and aspartic acid were prominent in *** decrease in content of hypoxanthine(Hx)was found from 1 M(1-month soy sauce)to7 M(7-months soy sauce).Furthermore,a significant opposite tendency for changes between some volatile compounds and amino acids was indicated that there was a transformation between these two ***,a better understanding to the influence of fermentation time on soy sauce had been *** the formation and conversion of amino acids and sugars might be mainly responsible for flavor formation in CTFSS,the ratio of these two reactions rate led the metabolism to be divided into three steps,degradation,conversion and balance.

关键词： Soy sauce Fermentation Amino acids Volatile compounds Flavor Metabolomics

Three-dimensional printing technology for treatment of intracranial aneurysm

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Chinese Neurosurgical Journal 2016年第3期2卷 -页

作者： Yin Kang LiangHong Yu tao Xu ShuFa Zheng PeiSen Yao Man Liu yuanxiang Lin Department of Neurosurgery The First Affiliated Hospital Fujian Medical University Fuzhou 350005 Fujian Province China Bio-Manufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084China Medprin Regenerative Medical Technologies Company Limited Guangzhou 510000 Guangdong Province China

The development of three-dimensional (3D) printing technology provides a new method for surgical treatment, but currently there are few reports on its application in the treatment of aneurysm. The aim of the present study was to explore the materials and methods of fabricating 3D printed individual aneurysm model and its value in the treatment of intracranial aneurysm. Twenty-four patients with intracranial aneurysm diagnosed by CTA who had undergone operation in our hospital were analyzed retrospectively. CTA Data collected at the time of surgery was used for reconstruction. Soft Mimics 17.0 was used to reconstruct the thin layer CTA scan data into 3D image and the final data was sent to the 3D printer for fabricating the model. We compared the proposed 3D printed model-based preoperative plan and the actual approach used in the surgery based on CTA data to evaluate the value of the 3D printed model in preoperative planning, and picked out the materials which were more suitable for the clinic. Twenty-four aneurysm models with high degree of reality were fabricated successfully with 3D-printing technology. The patients’ blood vessels, skulls and aneurysms were printed into the reality model at a ratio of 1:1. It is reported that the soft material-based, 3D printed vascular and aneurysm model more closely resembled the characteristics of the real blood vessels, thus provides a better simulation compared to the plaster-based model. Compared with the original operation plan, 3D printed model could be used for pre-operative aneurysm clip selection, and provide more intuitive information in selection of operational approach. 3D printed model can be used as an operational physical model to design operative schemes, choose the best operative paths and select suitable aneurysm clips by its high simulation degree and individualized characteristics. The model is helpful for surgical planning, especially for the preoperative plan of treating refractory mult

关键词： 3D printed model Intracranial aneurysm Operation design

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NiO纳米碗状阵列的低压电阻开关特性

中国科学：物理学、力学、天文学 2023年第4期53卷 89-98页

作者：殷元祥谢雨莎陈涛张涛陈鹏邱晓燕西南大学物理科学与技术学院重庆400715 西南大学微纳结构光电子学重庆市重点实验室重庆400715

我们利用单层胶体球自组装模板结合电化学沉积和磁控溅射方法在覆盖Pt导电层的白云母衬底上制备了NiO纳米碗状阵列(Nano-bowl-like NiO Array,nb-NiO).每个NiO纳米碗高约450 nm,碗口直径约450 nm,碗深约300 nm,在Pt导电层上密排形成二... 详细信息

我们利用单层胶体球自组装模板结合电化学沉积和磁控溅射方法在覆盖Pt导电层的白云母衬底上制备了NiO纳米碗状阵列(Nano-bowl-like NiO Array,nb-NiO).每个NiO纳米碗高约450 nm,碗口直径约450 nm,碗深约300 nm,在Pt导电层上密排形成二维阵列.在nb-NiO阵列上覆盖厚约20 nm的HfO_(2)薄膜,然后制备Ag或Pt点电极形成Ag(Pt)/HfO_(2)/nb-NiO/Pt堆栈器件.器件在小于±0.4 V的翻转电压下即可在高低电阻态之间周期性可逆跳变,表现出良好的电阻开关特性:器件在高低电阻态之间翻转的时间10^(3).断电后,器件稳定在高(低)电阻态的时间>104s.纳米碗状阵列结构使得NiO薄膜厚度在150–450 nm内周期性连续变化;由于氧空位导电细丝更易在厚度较小的纳米碗底部导通,因此碗状阵列结构使得原本空间随机分布的氧空位导电细丝通道具有了局域有序性,进而提升了器件电阻开关性能的稳定性.HfO_(2)薄膜层降低了器件漏电流,提高了器件的高/低电阻值比.Ag电极与氧离子的氧化还原反应加速了氧空位导电细丝的导通和断裂,从而降低了翻转电压.

关键词： NiO纳米碗状阵列电阻开关特性低电压

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大鼠脊髓背角神经元内SP受体与Fos共存

解剖学报 1997年第4期28卷 346-349,I002页

作者：陶元祥李云庆赵志奇重本隆一金子武嗣水野升中国科学院上海脑研究所第四军医大学解剖学教研室日本京都大学医学部高级脑形态学研究室

用免疫细胞化学技术对大鼠脊髓背角内Ｆｏｓ阳性神经元和ＳＰ受体阳性神经元的分布及相互间关系进行了研究。一侧后肢跖部皮下注射２％福尔马林后，同侧脊髓背角内可见有大量的Ｆｏｓ阳性神经元，而对侧背角内未见或偶见少量Ｆｏｓ阳性... 详细信息

用免疫细胞化学技术对大鼠脊髓背角内Ｆｏｓ阳性神经元和ＳＰ受体阳性神经元的分布及相互间关系进行了研究。一侧后肢跖部皮下注射２％福尔马林后，同侧脊髓背角内可见有大量的Ｆｏｓ阳性神经元，而对侧背角内未见或偶见少量Ｆｏｓ阳性神经元。Ｆｏｓ阳性神经元主要集中分布于背角Ⅰ层和Ⅱ层背侧的内侧部。ＳＰ受体阳性的胞体和树突最密集分布区为背角的Ⅰ层，少量分布于Ⅱ层的背侧部和Ⅲ～Ⅳ层。Ⅱ层的腹侧部仅见来自Ⅰ层、Ⅱ层的背侧部和背角深层的阳性树突。双标记免疫反应结果显示，背角内部分Ｆｏｓ阳性神经元呈ＳＰ受体阳性，主要分布于Ⅰ层内侧半。上述结果提示，背角浅层内ＳＰ受体可能涉及伤害性刺激诱发的ｃ－ｆｏｓ表达。

关键词： SP受体 Fos共存脊髓背角免疫细胞化学神经元

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基于汽车RVC通信协议的调节器电路设计

微处理机 2023年第4期44卷 26-29页

作者：陶鑫罗源翔揣荣岩沈阳工业大学信息科学与工程学院沈阳110870 江苏奥尼克电气股份有限公司徐州221132

汽车用电设备随着汽车电子技术的快速发展不断丰富多样化,为实现更为稳定的汽车供电系统,保证汽车电子设备运行的高效与可靠性,基于对汽车供电系统工作原理的了解,对电压调节器进行集成化改进设计。改进后的电路具有带隙基准源、状态检... 详细信息

汽车用电设备随着汽车电子技术的快速发展不断丰富多样化,为实现更为稳定的汽车供电系统,保证汽车电子设备运行的高效与可靠性,基于对汽车供电系统工作原理的了解,对电压调节器进行集成化改进设计。改进后的电路具有带隙基准源、状态检测、比较器、过流及过热保护等模块。芯片通过接收由车载ECU传给调节器的RVC信号实现功率控制功能,并通过RVC信号控制电压调节器功能模块,使电压调节器的输出电压稳定在13.85~14.15V之间。经实际性能测试,验证电路功能参数符合预期。

关键词：电压调节器带隙基准源比较器