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Study on the green extraction of corncob xylan by deep eutectic solvent

Grain & Oil Science and Technology 2024年第1期7卷 50-59页

作者： Bingyu Jiao Le Wang Haitao Gui Zifu Ni Rong Du yuansen hu School of Biological Engineering National Engineering Research Center of Wheat and Corn Further ProcessingZhengzhou 450001China

Corn as one of the world's major food crops,its by-product corn cob is also rich in ***,the unreasonable utilization of corn cob often causes the environmental pollution,waste of resources and other *** one of the most abundant polymers in nature,xylan is widely used in food,medicine,materials and other *** cob is rich in xylan,which is an ideal raw material for extracting ***,the intractable lignin is covalently linked to xylan,which increases the difficulty of xylan *** has been reported that the deep eutectic solvent(DES)could preferentially dissolve lignin in biomass,thereby dissolving the ***,the xylan in the extract was separated by ethanol precipitation *** xylan precipitate was obtained after centrifugation,while the supernatant was *** components of the supernatant after ethanol precipitation were separated by the rotary *** ethanol,water and DES were collected for the subsequent extraction of corn cob *** this study,a novel way was provided for the green production of corn cob *** DES was used to extract xylan from corn cob which was used as the raw *** effects of solid-liquid ratio,reaction time,reaction temperature and water content of DES on the extraction rate of corn cob xylan were investigated by the single factor ***,the orthogonal test was designed to optimize the xylan extraction *** structure of corn cob xylan was analyzed and *** results showed that the optimum extraction conditions of corn cob xylan were as follows:the ratio of corn cob to DES was 1:15(g:mL),the extraction time was 3 h,the extraction temperature was 60℃,and the water content of DES was 70%.Under these conditions,the extraction rate of xylan was 16.46%.The extracted corn cob xylan was distinctive triple helix of polysaccharide,which was similar to the structure of commercially available *** was effectively and workably extracted from corn

关键词： Corncob Deep eutectic solvent Xylan Process optimization Extraction

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莱姆病螺旋体端粒解离酶的表达纯化及其晶体衍射分析

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西北农林科技大学学报（自然科学版） 2024年第2期52卷 1-11页

作者：胡元森王远吕扬勇黄亮张帅兵李娜河南工业大学生物工程学院河南郑州450001

【目的】对莱姆病螺旋体(Borrelia burgdorferi)端粒解离酶(telomere resolvase,ResT)进行原核可溶性表达纯化,并筛选高衍射度的ResT-DNA复合物晶体,为探究ResT-DNA复合物的结构和功能奠定基础。【方法】合成pET28 b-ResT表达质粒,构建... 详细信息

【目的】对莱姆病螺旋体(Borrelia burgdorferi)端粒解离酶(telomere resolvase,ResT)进行原核可溶性表达纯化,并筛选高衍射度的ResT-DNA复合物晶体,为探究ResT-DNA复合物的结构和功能奠定基础。【方法】合成pET28 b-ResT表达质粒,构建原核表达载体pSUMO-ResT,分析ResT可溶性蛋白在BL21(DE3)、BL21(DE3)pLysS、BL21 Gold(DE3)pLysS、BL21 Codon Plus(DE3)、Rosetta(DE3)和Rosetta(DE3)pLysS中的表达量,获得最优的ResT原核表达菌株。基于AlphaFold预测的ResT蛋白模型,利用定点突变及ResT可溶性蛋白表达分析,获得高效可溶性表达ResT突变体。经Ni-NTA亲和层析、HiTrap Heparin HP亲和层析及分子筛层析对ResT突变体ResT(W94H)进行蛋白纯化。利用EMAS检测ResT(W94H)与DNA的结合能力。使用蛋白晶体接种(seeding)和交叉接种(cross-seeding)方法,利用多种商业化结晶试剂盒筛选并优化ResT-DNA复合物的结晶条件,对复合物晶体进行X射线衍射分析。【结果】BL21 Gold(DE3)pLysS菌株是ResT最佳的高效可溶性表达菌株,W94H突变体比野生型具有更强的可溶性表达。获得了高纯度(95%)且高质量浓度(15 mg/mL)的ResT(W94H),其能够与底物DNA形成稳定的ResT-DNA复合物。在18℃下,ResT-DNA最佳结晶条件是:PEG3350150 g/L、二甲苯二酸钠0.1 mol/L(pH 6.6)、NaCl 0.15 mol/L,ResT-DNA晶体分辨率最高为3.52,晶体空间群为P4。【结论】得到了高纯度ResT(W94H)蛋白,获得了ResT-DNA复合物晶体。

关键词：莱姆病螺旋体端粒解离酶原核表达和纯化蛋白结晶 X-射线衍射

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转录因子Afndt6对黄曲霉毒力和侵染力的影响

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河南工业大学学报（自然科学版） 2024年第3期45卷 9-16页

作者：兰海儿梁柳柯魏闪雷阳张帅兵胡元森吕扬勇河南工业大学生物工程学院河南郑州450001

为探究转录因子Afndt6对黄曲霉生长发育、毒素生物合成及侵染粮食籽粒能力的影响,通过同源重组构建Afndt6基因缺失的黄曲霉菌株,采用体视显微镜和切片观察手段研究Afndt6基因缺失对黄曲霉生长发育和孢子产生的影响,采用薄层色谱层析测... 详细信息

为探究转录因子Afndt6对黄曲霉生长发育、毒素生物合成及侵染粮食籽粒能力的影响,通过同源重组构建Afndt6基因缺失的黄曲霉菌株,采用体视显微镜和切片观察手段研究Afndt6基因缺失对黄曲霉生长发育和孢子产生的影响,采用薄层色谱层析测定黄曲霉毒素生物合成量,以花生和玉米籽粒为基质评价基因缺失菌株的侵染力。结果表明:与对照菌株相比,ΔAfndt6菌株的生长速度、分生孢子的产量没有显著变化,但菌落分生孢子颜色变为白色;基因缺失菌株对细胞壁胁迫试剂刚果红和氧化胁迫压力不敏感,200μg/mL刚果红处理后对照菌株和ΔAfndt6菌株的生长抑制率分别为31.10%和25.81%,15 mmol/L H_(2)O_(2)处理后对照菌株和ΔAfndt6菌株的生长抑制率分别为47.94%和17.05%;ΔAfndt6菌株的黄曲霉毒素产量显著升高,对花生、玉米种子的侵染能力增强。Afndt6基因编码的转录因子能够影响黄曲霉生长发育,负调控黄曲霉毒素的生物合成,可为储粮过程中防控黄曲霉及黄曲霉毒素污染提供参考。

关键词： Afndt6 黄曲霉黄曲霉毒素侵染力

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Effects of hexanal fumigation on fungal spoilage and grain quality of stored wheat

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Grain & Oil Science and Technology 2021年第1期4卷 10-17页

作者： Shuaibing Zhang Minghui Zheng huanchen Zhai Ping'an Ma Yangyong Lyu yuansen hu Jingping Cai College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

The prevention of fungal spoilage is an essential consideration in wheat *** studies have revealed that volatile organic compounds(VOCs),possibly with natural fungicidal properties,could be produced from stored wheat *** this study,the antifungal effect of hexanal,a main component of VOCs from stored wheat,against spoilage fungi on agar plate and in high-moisture wheat grains were investigated via the gas fumigation *** the impact of hexanal fumigation on grain quality was evaluated through analysis of the malondialdehyde content,fatty acid values,germination percentages and vigor of 16%and 18%moisture wheat grains fumigated with 1.66,2.49,and 3.31 mmol/L hexanal *** results of in vitro antifungal experiments on agar plates revealed that the minimum inhibitory concentration and minimum fungicidal(fatal)concentration of hexanal against the five fungi were 4–14 folds and 4–7 folds lower than those of propionic acid,*** fungal spoilage of high-moisture wheat grains inoculated with pure fungal spores and with naturally occurring fungi could be completely inhibited by 1.66 mmol/L hexanal *** 5-week storage of high-moisture wheat grains fumigated with 1.66,2.49,and 3.31 mmol/L hexanal vapor,the malondialdehyde content in high-moisture wheat grains did not change significantly in all samples,and fatty acid values were slightly higher in 18%moisture wheat than in 16%moisture *** germination percentages and vigor of wheat samples decreased with increased hexanal vapor concentrations and moisture *** results indicated that hexanal fumigation could be used as an alternative chemical control method to prevent the fungal spoilage of postharvest wheat.

关键词： Hexanal Chemical control Spoilage fungi Wheat storage Grain quality

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Insights into the mechanism of Sub3 inhibiting Fusarium moniliforme infection in maize

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Grain & Oil Science and Technology 2022年第1期5卷 22-34页

作者： Haojie Yang Wei Zhang Pingping Tian Bangbang Li Shan Wei Shuaibing Zhang Na Li Yangyong Lyu yuansen hu College of Biological Engineering Henan University of TechnologyZhengzhou 450001China Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat Zhengzhou 450001China

Fusarium moniliforme(***) and its secondary metabolite fumonisin pose a severe threat to food safety,and searching for effective antimicrobial agents is a focus of current *** this study,the secondary structure of Sub3 was analyzed by circular dichroism,meanwhile,the inhibition rate of Sub3 against spores,mycelia of *** and infected maize was *** explore the possible inhibition mechanisms,morphological and structural changes of spores treated with Sub3 at0,1/2 MIC(minimum inhibitory concentration) and MIC were observed by scanning electron microscopy and transmission electron microscopy;the cell wall integrity,membrane integrity,reactive oxygen species,mitochondrial membrane potential,ATP synthase activity,redox reactions,and the nuclear damage of *** were also *** results showed that Sub3 was mostly in the state of random in deionized water,while mainly showed the β-sheet structure in the hydrophobic environment of 50% Trifluoroethanol(TFE) solution,indicating that Sub3 might generate partial structure deformation when acting on the cell membrane;and its MIC on *** spores was 0.2 g/*** the 1/2 MIC and MIC,the inhibition rates of Sub3 against *** infected maize were 34.3% and75.6%,*** results of inhibition mechanisms revealed that the defective pathogenicity of *** caused by Sub3 was attributed to damages on both the cell wall and the cell membrane,which might upset balance of intracellular redox system and mitochondrial energy metabolism and trigger nucleus damage,ultimately leading to cell ***,Sub3 could diminished ATP synthase enzyme activity in a dose-dependent *** results provided direct evidence for inhibition of *** infection of maize by Sub3,and useful knowledge applicable for food preservation.

关键词： Sub3 Antifungal activity Inhibition mechanism F.moniliforme Maize

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Survey of Intracellular Protein Extraction Methods from Pichia pastoris

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World Journal of Engineering and Technology 2015年第4期3卷 1-6页

作者： Yuli Wang yuansen hu Yang Lei Yangyong Lv Le Wang Hongwei Wei Bioengineering College Henan University of Technology Zhengzhou China

The broken efficiency of cell wall and the release amount of Pichia pastoris intracellular protein under different cell breaking conditions were investigated in this paper. The results showed that broken efficiency using hot alkali combined with high-pressure homogenizing method was higher than that of enzyme hydrolysis, hot alkali treatment and high-pressure homogenation, respectively. Suspended medium had little effect on the broken efficiency of yeast cell, but had significant effect on the protein release yield. The results indicated that optimal condition for intracellular proteins extraction was 30% (wet weight, w/v) of yeast cells suspend in 50 mM phosphate buffer (pH 10.0), water bathed at 60?C for 2 hours, homogenized twice at 100 MPa pressure. The broken efficiency of Pichia pastoris cell could reach 87.6% and the protein yield was 35.48 g per 100 g cells.

关键词： Pichia pastoris High Temperature and Alkali Treatment High Pressure Homogeniza-Tion Cell Wall Breaking Protein Extraction

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Anti-fungal properties of Puroindoline A against Aspergillus glaucus

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Grain & Oil Science and Technology 2020年第1期3卷 1-8页

作者： Wenjing Yuan Pingping Tian Ang Lyu Yangyong Lyu Wei Zhang Shan Wei yuansen hu College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

Aspergillus glaucus can grow in low moisture grain,and is one of the main fungi responsible for agricultural product *** A(PINA)is a tryptophan-rich alkaline adiponectin that can effectively inhibit numerous plant bacteria and ***,the mechanism of PINA against *** remains ***,we found that recombinant PINA(rPINA)could inhibit *** mycelia growth on salt Czapek dox agar(SCDA)medium and spore germination on Czapek dox(CD)*** electron microscopy revealed that incomplete morphological characteristics of both *** spores and mycelia occurred following rPINA *** scanning confocal microscopy(LSCM)showed that rPINA could enter the interior of *** cytometry and propidium iodide(PI)staining illustrated membranes of spores were severely damaged,especially after treatment with 0.9 mg/mL rPINA for 12 h,and spores with intact membranes were reduced by 30.7%.Additionally,rPINA reduced the mitochondrial membrane potential(MMP)by 5,5′,6,6′-tetrachlorr-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide(JC-1)staining,and caused DNA damage by 4′,6-diamidino-2-phenylindole(DAPI)*** results indicated that rPINA may damage cell membranes and DNA structure and reduceMMP,thereby inhibiting the growth of *** antifungal mechanism has been demonstrated in this study,and results show that rPINA has application potential in preventing postharvest loss in the agricultural industry.

关键词： Puroindoline A Aspergillus glaucus Membrane integrity Mitochondrial membrane potential DNA damage

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Synthesis of High Ion Conductivity Cubic Garnet Li7La3Zr2O12 Solid Electrolyte by Controlling the 8a Oxygen Vacancy

Synthesis of High Ion Conductivity Cubic Garnet Li7La3Zr2O12...

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2020中国汽车工程学会年会暨展览会

作者： Liu Min hu yuansen Shi Jianpeng Cao Yuanpu Gong Yu Department of Technology Centre of Dongfeng Motor Group Co.Ltd.

We present a novel strategy to synthesize pure cubic garnet LiLaZrO solid electrolyte at a relatively low temperature(700℃) by two *** the process of obtaining LiLaZrO,the 8 a oxygen vacancy in pyrochlore of LaZrO was used to occupy the "Li1" 8 a site which the information of tetragonal phase LiLaZrO requires lithium *** lithium ion conductivity of the obtained cubic garnet LiLaZrO as high as 1.7×10 S cm at room temperature is consistent with the order of record conductivity in the literatures.

关键词： cubic garnet solid electrolyte oxygen vacancy ion conductivity

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Consolidated bioprocess of corn stover to polysaccharide using Chaetomium globosum CGMCC 6882

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Grain & Oil Science and Technology 2019年第1期2卷 11-14页

作者： Lin Peng Tao Ning Wenbo Lu Peizhang Chen Haifeng Li Yanjie Yi Zichao Wang yuansen hu National Engineering Laboratory for Cereal Fermentation Technology Jiangnan UniversityWuxi 214122China College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

Polysaccharide produced from medicinal endophytic fungus not only has applications in foods,but also exhibits multiple biological *** this work,an endophytic fungus Chaetomium globosum CGMCC 6882 could use corn stover to produce a polysaccharide(GCP-SC)by consolidated bioprocess and the titer of GCP-SC reached 3.2 g/*** transcriptional levels of genes related to cellulose degradation(cbh,cdh,glu and egl)in *** CGMCC 6882 were 4.38,3.85,3.13,and 2.17 folds compared to the control group when corn stover was used as the sole carbon ***,GCP-SC showed a time-and dose-dependent manner of inhibitory effect on A549 cells and the inhibitory rate reached 93.3%.This work provides meaningful data on agricultural residues utilization and facilitation of future relative resource conversion studies.

关键词： Corn stover Chaetomium globosum CGMCC 6882 Polysaccharide

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一种新颖的光通信方式-光频多路复用载波通信

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光通信技术 1989年第3期13卷 9-14页

作者：原荣胡远森机械电子工业部第三十四研究所

本文根据某些文献报导,首先将光频多路复用(OFDM)载波通信与波分复用(WDM)和相干光通信作了简单的比较,然后对其工作原理和解复用的关键器件——光纤法布里-珀罗(OFFP)调谐滤波器作了说明,最后以两个实验网络阐明其实现过程,指出所具有... 详细信息

本文根据某些文献报导,首先将光频多路复用(OFDM)载波通信与波分复用(WDM)和相干光通信作了简单的比较,然后对其工作原理和解复用的关键器件——光纤法布里-珀罗(OFFP)调谐滤波器作了说明,最后以两个实验网络阐明其实现过程,指出所具有的通信潜力和广泛的应用前景。

关键词：光通信光频复用载波通信光滤波

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