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Comparing the intestinal bacterial communies of sympatric wintering Hooded Crane (Grus monacha) and Domestic Goose(Anser anser domesticus)

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Avian Research 2020年第2期11卷 106-114页

作者： Rong Fu Xingjia Xiang yuanqiu dong Lei Cheng Lizhi Zhou School of Resources and Environmental Engineering Anhui UniversityHefei 230601China Anhui Province Key Laboratory of Wetland Ecological Protection and Restoration(Anhui University) Hefei 230601China

Background:Gut microbiota play crucial roles in host *** birds and domestic poultry often occupy sympatric habitats,which facilitate the mutual transmission of intestinal ***,the distinct intestinal microbial communities between sympatric wild birds and poultry remain *** present,the risk of interspecies transmission of pathogenic bacteria between wild and domestic host birds is also a research ***:This study compared the intestinal bacterial communities of the overwintering Hooded Crane(Grus monacha)and the Domestic Goose(Anser anser domesticus)at Shengjin Lake,China,using Illumina high-throughput sequencing technology(Mi-Seq platform).Results:Our results revealed that Firmicutes,Actinobacteria,Proteobacteria,Bacteroidetes and Chloroflexi were the dominant bacterial phyla in both *** gut bacterial community composition differed significantly between sympatric Hooded Cranes and Domestic ***,the hosts exhibited little variation in gut bacterial *** relative abundance of Firmicutes was significantly higher in the guts of the Hooded Cranes,while the relative abundances of Actinobacteria,Proteobacteria,Bacteroidete and Chloroflexi were significantly higher in guts of Domestic ***,a total of 132 potential pathogenic operational taxonomic units(OTUs)were detected in guts of Hooded Cranes and Domestic Geese,and 13 pathogenic OTUs(9.8%)were found in both host *** bacterial community composition and diversity differed significantly between ***:The results showed that the gut bacterial community composition differs significantly between sympatric Hooded Cranes and Domestic *** addition,potential pathogens were detected in the guts of both Hooded Cranes and Domestic Geese,with 13 pathogenic OTUs overlapping between the two hosts,suggesting that more attention should be paid to wild birds and poultry that might increase the risk of disease transmission in conspecific

关键词： High-throughput sequencing Hooded Crane Intestinal microbiota Poultry Pathogen

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安徽升金湖越冬白头鹤(Grus monacha)肠道菌群组成的时间变化

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湖泊科学 2017年第3期29卷 670-677页

作者：赵广红周立志董元秋宋昀微安徽大学资源与环境工程学院合肥230601 安徽升金湖国家级自然保护区管理局池州247200

肠道微生物对于维持动物消化道内环境的稳定具有重要意义,而肠道微生物菌群结构也受寄主食物成份等外界环境因素的影响.研究动物肠道微生物菌群结构可以更好地了解动物生活状况.以升金湖越冬白头鹤的粪便作为研究对象,以微生物16S rRNA... 详细信息

肠道微生物对于维持动物消化道内环境的稳定具有重要意义,而肠道微生物菌群结构也受寄主食物成份等外界环境因素的影响.研究动物肠道微生物菌群结构可以更好地了解动物生活状况.以升金湖越冬白头鹤的粪便作为研究对象,以微生物16S rRNA的V3~V4区作为标记基因,进行高通量测序,分析升金湖越冬不同时期白头鹤肠道菌群结构的差异性.研究结果表明:升金湖越冬期不同时期白头鹤粪便样品中微生物多样性指数如香农威纳多样性指数和辛普森指数无显著性差异.但是越冬期不同阶段的粪便样品中菌群组成存在差异,主要包括:在门分类水平,越冬前期拟杆菌门含量较高,厚壁菌门含量较中后期低;越冬中期放线菌门含量较高;越冬后期变形菌门含量较高.在属分类水平,相对于其他两个时期,越冬前期普氏菌属(Prevotella)含量较高,越冬中期杆菌属(Lysinibacillus)含量较高,越冬后期梭菌属(Clostridium)含量较高,并且普氏菌属含量从越冬前期到中期、再到后期有明显的下降趋势,而梭菌属含量从越冬前期到中期、再到后期有明显的升高趋势.这些结果表明不同越冬时期白头鹤肠道菌群组成不同,越冬期不同阶段的食物变化可能作为一个影响因素.

关键词：白头鹤肠道菌群组成越冬期 16S rRNA 升金湖

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Ligand binding and structural characterization of the C-terminal domain of Marine α-Amylase AmyP indicate a novel starch binding domain

Ligand binding and structural characterization of the C-term...

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The Ninth National Symposium on Enzyme Engineering

作者： Jigang Yu Hui Peng yuanqiu dong Ying Wang Xiaoming Tu Yazhong Xiao Xuecheng Zhang School of Life Sciences Anhui University Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis School of Life Sciences University of Science andTechnology of China

Starch binding domains（SBDs）are important for the functions ofα-amylases and potential in the application in biotechnology and *** is a newly identified marine microbialα-amylase,which was attributed to a new subfamily of glycoside hydrolysis enzyme family 13 and exhibited some unique properties *** degradation to rice raw *** sequence analysis indicated that at the C-terminus of AmyP might be an *** titration calorimetry（ITC）and nuclear magnetic resonance（NMR）chemical shift perturbation assays demonstrated the capability of the C-terminal domain of AmyP to bind to soluble starch and its analogueβ-cyclodextrin（β-CD）,supporting its SBD *** characterization by circular dichroism（CD）,intrinsic fluorescence,and NMR showed that free form AmyP-SBD adopted a flexible state composed of interconverting well folded conformations,which converged to form a unique tertiary structure when binding to the *** results may help elucidate the function mechanism of AmyP and facilitate exploring its application potential.

关键词： α-Amylase AmyP binding induced folding starch binding domain structure.

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Effect of Senecio scandens ethanol extract on gut microbiota composition in mice

Effect of Senecio scandens ethanol extract on gut microbiota...

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第十六届中国实验动物科学年会

作者： Gang Yao Hui Zhang Guoyong Luo Zuhua Wang Hai Zhao Jian Zhang yuanqiu dong Yuan Gao Shuguang Wu Institute of Laboratory Animal Science Guizhou University of Traditional Chinese Medicine College of Pharmaceutical Sciences Guizhou University of Traditional Chinese Medicine

The gut microbiota inhabits the animal intestinal tract,and dysbiosis of the gut microbiota may result in *** scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese *** of *** are reported to show strong antimicrobial activity,and quercetin significantly decreases some species in the caecal ***,the bactericidal effects of the extracts on the gut microbiota remain ***,we supplied ethanol extract of ***,which might possibly be used as an alternative for chemical antibiotics,to mice to investigate the state of the intestinal *** studies included control group,low-,moderate-,and high-dose ethanol extract groups,and cefixime capsule *** ethanol extract groups did not present reduced diversity or differences in the gut microbiota *** were significant differences between the ethanol extract and cefixime capsule groups in terms of the gut *** control and ethanol extract groups contained similar bacteria,which suggested that the ethanol extract has no inhibitory effect on the gut microbiota in *** and Lactobacillus acidophilus were significantly increased in the high-dose *** secretory immunoglobulin A and mucin 2 concentrations increased as the dose of ethanol extract *** functional prediction differences between the control and ethanol extract groups decreased with increasing extract doses,which indicated that the low-dose and high-dose extract treatments might regulate different pathways and functions of the gut *** results also highlighted the prevention of bacterial drug resistance in the ethanol extract groups.

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Resonance mechanism of flapping wing based on fluid structure interaction simulation

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Chinese Journal of Aeronautics 2024年第5期37卷 243-262页

作者： Yueyang GUO Wenqing YANG Yuanbo dong dong XUE National Key Laboratory of Science and Technology on Aerodynamic Design and Research School of AeronauticsNorthwestern Polytechnical UniversityXi'an 710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518063China Yangtze River Delta Research Institute of Northwestern Polytechnical University Taicang 215400China

Certain insect species have been observed to exploit the resonance mechanism of their *** order to achieve resonance and optimize aerodynamic performance,the conventional approach is to set the flapping frequency of flexible wings based on the Traditional Structural Modal(TSM)***,there exists controversy among researchers regarding the relationship between frequency and aerodynamic *** that the structural response of wings can be influenced by the surrounding air vibrations,an analysis known as Acoustic Structure Interaction Modal(ASIM)is introduced to calculate the resonant *** this study,Fluid Structure Interaction(FSI)simulations are employed to investigate the aerodynamic performance of flapping wings at modal frequencies derived from both TSM and ASIM *** performance is evaluated for various mass ratios and frequency ratios,and the findings indicate that the deformation and changes in vortex structure exhibit similarities at mass ratios that yield the highest aerodynamic ***,the flapping frequency associated with the maximum time-averaged vertical force coefficient at each mass ratio closely aligns with the ASIM frequency,as does the frequency corresponding to maximum ***,the ASIM analysis can provide an effective means for predicting the optimal flapping frequency for flexible ***,it enables the prediction that flexible wings with varying mass ratios will exhibit similar deformation and vortex structure *** paper offers a fresh perspective on the ongoing debate concerning the resonance mechanism of Flexible Flapping Wings(FFWs)and proposes an effective methodology for predicting their aerodynamic performance.

关键词： Flexible Flapping Wing(FFW) Acoustic Structure Interaction Modal(ASIM) Fluid Structure Interaction(FSI) Resonance mechanism Aerodynamic performance

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Copper slag assisted coke reduction of phosphogypsum for sulphur dioxide preparation

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Chinese Journal of Chemical Engineering 2024年第1期65卷 43-53页

作者： dong Ma Qinhui Wang State Key Laboratory of Clean Energy Utilization Institute for Thermal Power EngineeringZhejiang UniversityHangzhou 310027China

The reduction of phosphogypsum(PG)to lime slag and SO_(2)using coke can effectively alleviate the environmental problems caused by ***,the PG decomposition temperature remains high and the product yield remains *** adding additives,the decomposition temperature can be further reduced and PG decomposition rate and product yield can be ***,the use of current additives such as Fe_(2)O_(3)and SiO_(2)brings the problem of increasing economic ***,it is proposed to use solid waste copper slag(CS)as a new additive to reduce PG to prepare SO2,which can reduce the cost and meet the environmental benefits at the same *** effects of proportion,temperature and thermostatic time on PG decomposition are investigated by experimental and kinetic analysis combined with FactSage thermodynamic calculations to optimize the roasting ***,the reaction mechanism is *** is found that adding CS to the coke and PG system can increase the rate of PG decomposition and SO_(2)yield while lowering the PG decomposition *** example,when the CS/PG mass ratio increases from 0 to 1,PG decomposition rate increases from 83.38%to 99.35%,SO_(2)yield increases from 78.62%to 96.81%,and PG decomposition temperature decreases from 992.4℃to 949.6℃.The optimal reaction parameters are CS/PG mass ratio of 1,Coke/PG mass ratio of 0.06 at 1100℃for 20 min with 99.35%PG decomposition rate and 96.81%SO_(2) *** process proceeds according to the following reactions:2CaSO_(4)+ 0.7C + 0.8Fe_(2)SiO_(4)→0.8Ca_(2)SiO_(4)+ 0.2Ca_(2)Fe_(2)O_(5)+ 0.4Fe_(3)O_(4)+2SO_(2)+ 0.7CO_(2)Finally,a process for decomposing PG with coke and CS is proposed.

关键词： Phosphogypsum Sulfur dioxide Copper slag Fluidized-bed Reduction Waste treatment

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Finite-time Prescribed Performance Time-Varying Formation Control for Second-Order Multi-Agent Systems With Non-Strict Feedback Based on a Neural Network Observer

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IEEE/CAA Journal of Automatica Sinica 2024年第4期11卷 1039-1050页

作者： Chi Ma Dianbiao dong the School of Mechanical Engineering Northwestern Polytechnical UniversityXi’an 710072China

This paper studies the problem of time-varying formation control with finite-time prescribed performance for nonstrict feedback second-order multi-agent systems with unmeasured states and unknown *** eliminate nonlinearities,neural networks are applied to approximate the inherent dynamics of the *** addition,due to the limitations of the actual working conditions,each follower agent can only obtain the locally measurable partial state information of the leader *** address this problem,a neural network state observer based on the leader state information is ***,a finite-time prescribed performance adaptive output feedback control strategy is proposed by restricting the sliding mode surface to a prescribed region,which ensures that the closed-loop system has practical finite-time stability and that formation errors of the multi-agent systems converge to the prescribed performance bound in finite ***,a numerical simulation is provided to demonstrate the practicality and effectiveness of the developed algorithm.

关键词： Finite-time control multi-agent systems neural network prescribed performance control time-varying formation control

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Resilient control of flexible hypersonic vehicles against unmatched distributed faults

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Chinese Journal of Aeronautics 2024年第5期37卷 60-70页

作者： dong ZHAO Wenjing REN School of Artificial Intelligence Anhui UniversityHefei 230601China Key Laboratory of System Control and Information Processing Ministry of EducationShanghai Jiao Tong UniversityShanghai 200240China School of Electrical Engineering and Automation Hefei University of TechnologyHefei 230009China

This paper studies a robust fault compensation and vibration suppression problem of flexible hypersonic *** controlled plant is represented by a cascade system composed of a nonlinear Ordinary Differential Equation(ODE)and an Euler-Bernoulli Beam Equation(EBBE),in which the vibration dynamics is coupled with the rigid dynamics and suffers from distributed faults.A state differential transformation is introduced to transfer distributed faults to an EBBE boundary and a longitudinal dynamics is refined by utilizing T-S fuzzy IF-THEN rules.A novel T-S fuzzy based fault-tolerant control algorithm is developed and related stability conditions are *** robust exponential stability and well-posedness are proved by using the modified l_(0)-semigroup based Lyapunov direct approach.A simulation study on the longitudinal dynamics of flexible hypersonic vehicles effectively verifies the validity of the developed theoretical results.

关键词： Flexible hypersonic vehicles ODE-EBBE cascade Robust exponential stability Unmatched distributed fault compensation Vibration suppression

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Modeling urban scale human mobility through big data analysis and machinelearning

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Building Simulation 2024年第1期17卷 3-21页

作者： Yapan Liu Bing dong Department of Mechanical&Aerospace Engineering Syracuse University263 Link HallSyracuseNY 13244USA

In the United States,the buildings sector consumes about 76%of electricity use and 40% of all primary energy use and associated greenhouse gas *** behavior has drawn increasing research interests due to its impacts on the building energy ***,occupant behavior study at urban scale remains a challenge,and very limited studies have been *** an effort to couple big data analysis with human mobility modeling,this study has explored urban scale human mobility utilizing three months Global Positioning System(GPS)data of 93,o00 users at Phoenix Metropolitan *** research extracted stay points from raw data,and identified users'home,work,and other locations by Density-Based Spatial Clustering ***,daily mobility patterns were constructed using different types of *** propose a novel approach to predict urban scale daily human mobility patterns with 12-hour prediction horizon,using Long Short-Term Memory(LSTM)neural network *** shows the developed models achieved around 85%average accuracy and about 86%mean *** developed models can be further applied to analyze urban scale occupant behavior,building energy demand and flexibility,and contributed to urban planning.

关键词： urban human mobility big data analysis urban scale occupant behavior recurrent neural networks

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Potential reduction in carbon fixation capacity under climate change in a Pinus koraiensis forest

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Forest Ecosystems 2024年第2期11卷 152-161页

作者： dong Kook Woo Department of Civil Engineering Keimyung UniversityDaegu42601Republic of Korea

There has been an increasing recognition of the crucial role of forests, responsible for sequestering atmospheric CO_(2), as a moral imperative for mitigating the pace of climate change. The complexity of evaluating climate change impacts on forest carbon and water dynamics lies in the diverse acclimations of forests to changing environments. In this study, we assessed two of the most common acclimation traits, namely leaf area index and the maximum rate of carboxylation(V_(cmax)), to explore the potential acclimation pathways of Pinus koraiensis under climate change. We used a mechanistic and process-based ecohydrological model applied to a P. koraiensis forest in Mt. Taehwa, South Korea. We conducted numerical investigations into the impacts of(i) Shared Socioeconomic Pathways 2–4.5(SSP2-4.5) and 5–8.5(SSP5-8.5),(ii) elevated atmospheric CO_(2) and temperature, and(iii) acclimations of leaf area index and V_(cmax)on the carbon and water dynamics of P. koraiensis. We found that there was a reduction in net primary productivity(NPP) under the SSP2-4.5 scenario, but not under SSP5-8.5, compared to the baseline, due to an imbalance between increases in atmospheric CO_(2) and temperature. A decrease in leaf area index and an increase in V_(cmax)of P. koraiensis were expected if acclimations were made to reduce its leaf temperature. Under such acclimation pathways, it would be expected that the well-known CO_(2) fertilizer effects on NPP would be attenuated.

关键词： Climate change NEP Pinus koraiensis Warming Acclimation

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